AccaA.19900.a

Glucokinase

CENTER ID:	AccaA.19900.a
ORGANISM:	Acanthamoeba castellanii
ASSOCIATED DISEASE:	Amoebic keratitis and encephalomyelitis
CURRENT STATUS:	in PDB
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AccaA.19900.a.D11.PD38387	obsolete		1	352

Structures

8DTC

DEPOSITED: 7/25/2022

DETERMINATION: XRay

Diffraction Image

CLONE: AccaA.19900.a.Q2.GE42882

PROTEIN: AccaA.19900.a.Q2.PS38640

Molecular models

METHOD	RESULTS
comparative modelling	Robetta_10690

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	AmoebaDB:ACA1_177380
UniProt:	L8GT25

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTSAPPLPIS TLVDIVEEAR PALLQWKDRF KESLKYFVGV DIGGTNTRVA LDTGSSDESY VQIAKFRASS SKHIVEGLQQ VARQVADILG VPASGACLAG AGRISDDGLS LDITNYPGTP ADRTLTSDQL PTYLFPADAT HFINDLESTC YGLKSLNESQ QLSSFFKPLW STSDTVTMER ANYLVLAVGT GLGIALLTSL GRGSRNIPLQ VMPMEFGHAL YSPATDPSKK DEEDRLAAYL SKTLYSGKHA IEYEDIVSGR GVLAVYQWIT AEHKEAAKYE SAEEVSTAAF REDPCPFATK ALLIHYRFLM RVAKNLCVGL QAKGMFLAGD NQVVNNPFFE KHLPEMRAEF LDHPKPDWID MVELYTQTES FNINLHGALY YARTDQR

NT Sequence

ATGACCAGCG CTCCGCCACT TCCCATCTCC ACGCTCGTTG ACATTGTCGA GGAGGCTCGG CCGGCCCTCC TTCAGTGGAA GGATCGATTC AAGGAGAGCC TGAAGTACTT CGTGGGTGTC GATATTGGTG GCACCAACAC GCGCGTGGCG CTCGACACCG GCTCCTCCGA CGAGTCCTAC GTCCAGATCG CCAAGTTCAG GGCGTCGAGC AGCAAGCACA TCGTTGAGGG ACTCCAGCAG GTCGCCCGTC AGGTGGCCGA CATCCTCGGC GTCCCTGCCT CGGGCGCCTG TCTTGCCGGC GCCGGCCGCA TCAGCGACGA TGGCCTCTCG CTTGATATCA CCAACTACCC CGGTACCCCT GCCGACCGCA CGCTGACCTC GGATCAGCTC CCGACCTACC TGTTCCCCGC TGATGCGACA CACTTCATCA ATGACCTCGA AAGCACGTGC TACGGCCTCA AGTCCCTTAA CGAGAGCCAG CAGCTCTCCT CCTTCTTCAA GCCCCTCTGG TCCACCTCGG ACACCGTCAC CATGGAGCGC GCCAACTATT TGGTGTTGGC CGTGGGCACT GGTCTGGGCA TCGCCCTCCT GACCTCGCTC GGCCGCGGCT CGCGCAACAT CCCGCTGCAG GTGATGCCCA TGGAGTTCGG CCACGCCCTC TACTCGCCGG CCACCGACCC CTCCAAGAAG GACGAGGAGG ACCGGCTCGC GGCCTACCTC TCGAAGACTC TCTACAGCGG CAAGCACGCC ATCGAGTACG AGGACATCGT ATCGGGCCGC GGCGTCCTGG CCGTCTACCA GTGGATCACC GCCGAGCACA AGGAGGCTGC CAAGTATGAG TCGGCCGAGG AGGTCAGCAC GGCCGCCTTC CGCGAGGACC CGTGCCCCTT CGCCACCAAG GCGCTGCTCA TCCACTACAG GTTCCTGATG CGTGTGGCCA AGAACCTCTG CGTGGGGCTG CAGGCCAAGG GCATGTTCCT TGCCGGTGAC AACCAGGTGG TAAACAACCC CTTCTTCGAG AAGCACCTGC CGGAGATGAG GGCCGAGTTT TTGGATCACC CCAAGCCCGA CTGGATCGAC ATGGTGGAGC TCTACACCCA GACGGAGAGC TTCAACATCA ACCTCCACGG CGCCCTCTAC TACGCGCGTA CCGACCAGCG CTGA

Details for AccaA.19900.a.D11.PD38387

PURIFICATION DATe:	8/19/2019
CONCENTRATION:	20mg/ml
OBSERVED MW:	50kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25mM tris pH 8.0, 200mM NaCl, 1mM TCEP
EXPRESSION HOST:	BL21 DE3
VIAL COUNT (approx.):	31
VIAL VOLUME:	100µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGSMTS APPLPISTLV DIVEEARPAL LQWKDRFKES LKYFVGVDIG GTNTRVALDT GSSDESYVQI AKFRASSSKH IVEGLQQVAR QVADILGVPA SGACLAGAGR ISDDGLSLDI TNYPGTPADR TLTSDQLPTY LFPADATHFI NDLESTCYGL KSLNESQQLS SFFKPLWSTS DTVMPMEFGH ALYSPATDPS KKDEEDRLAA YLSKTLYSGK HAIEYEDIVS GRGVLAVYQW ITAEHKEAAK YESAEEVSTA AFREDPCPFA TKALLIHYRF LMRVAKNLCV GLQAKGMFLA GDNQVVNNPF FEKHLPEMRA EFLDHPKPDW IDMVELYTQT ESFNINLHGA LYYARTDQR

Full NT Sequence (Expression Vector + Insert)

TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AATTAATTCT TAGAAAAACT CATCGAGCAT CAAATGAAAC TGCAATTTAT TCATATCAGG ATTATCAATA CCATATTTTT GAAAAAGCCG TTTCTGTAAT GAAGGAGAAA ACTCACCGAG GCAGTTCCAT AGGATGGCAA GATCCTGGTA TCGGTCTGCG ATTCCGACTC GTCCAACATC AATACAACCT ATTAATTTCC CCTCGTCAAA AATAAGGTTA TCAAGTGAGA AATCACCATG AGTGACGACT GAATCCGGTG AGAATGGCAA AAGTTTATGC ATTTCTTTCC AGACTTGTTC AACAGGCCAG CCATTACGCT CGTCATCAAA ATCACTCGCA TCAACCAAAC CGTTATTCAT TCGTGATTGC GCCTGAGCGA GACGAAATAC GCGATCGCTG TTAAAAGGAC AATTACAAAC AGGAATCGAA TGCAACCGGC GCAGGAACAC TGCCAGCGCA TCAACAATAT TTTCACCTGA ATCAGGATAT TCTTCTAATA CCTGGAATGC TGTTTTCCCG GGGATCGCAG TGGTGAGTAA CCATGCATCA TCAGGAGTAC GGATAAAATG CTTGATGGTC GGAAGAGGCA TAAATTCCGT CAGCCAGTTT AGTCTGACCA TCTCATCTGT AACATCATTG GCAACGCTAC CTTTGCCATG TTTCAGAAAC AACTCTGGCG CATCGGGCTT CCCATACAAT CGATAGATTG TCGCACCTGA TTGCCCGACA TTATCGCGAG CCCATTTATA CCCATATAAA TCAGCATCCA TGTTGGAATT TAATCGCGGC CTAGAGCAAG ACGTTTCCCG TTGAATATGG CTCATAACAC CCCTTGTATT ACTGTTTATG TAAGCAGACA GTTTTATTGT TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGAGATC CCGGTGCCTA ATGAGTGAGC TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA GCATTTGCAT GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT GCTCCACGCC CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGAGAC ATCAAGAAAT AACGCCGGAA CATTAGTGCA GGCAGCTTCC ACAGCAATGG CATCCTGGTC ATCCAGCGGA TAGTTAATGA TCAGCCCACT GACGCGTTGC GCGAGAAGAT TGTGCACCGC CGCTTTACAG GCTTCGACGC CGCTTCGTTC TACCATCGAC ACCACCACGC TGGCACCCAG TTGATCGGCG CGAGATTTAA TCGCCGCGAC AATTTGCGAC GGCGCGTGCA GGGCCAGACT GGAGGTGGCA ACGCCAATCA GCAACGACTG TTTGCCCGCC AGTTGTTGTG CCACGCGGTT GGGAATGTAA TTCAGCTCCG CCATCGCCGC TTCCACTTTT TCCCGCGTTT TCGCAGAAAC GTGGCTGGCC TGGTTCACCA CGCGGGAAAC GGTCTGATAA GAGACACCGG CATACTCTGC GACATCGTAT AACGTTACTG GTTTCACATT CACCACCCTG AATTGACTCT CTTCCGGGCG CTATCATGCC ATACCGCGAA AGGTTTTGCG CCATTCGATG GTGTCCGGGA TCTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AATTAATACG ACTCACTATA GGGGAATTGT GAGCGGATAA CAATTCCCCT CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACC ATGGGCCACC ACCACCACCA CCATAGCGGC GAAGTAAAAC CGGAAGTGAA GCCGGAGACC CACATCAACC TGAAGGTTAG CGACGGTAGC AGCGAAATCT TCTTCAAGAT TAAGAAGACC ACCCCGCTGC GTCGCCTGAT GGAAGCATTC GCTAAACGCC AGGGCAAGGA AATGGATTCC CTGCGCTTTC TGTACGACGG TATCCGTATT CAGGCAGACC AGACTCCGGA AGATCTGGAC ATGGAAGATA ACGACATTAT CGAAGCACAC CGTGAACAAA TCGGTGGATC CATGACCAGC GCGCCGCCGC TGCCGATCAG CACCCTGGTG GACATTGTTG AGGAAGCGCG TCCGGCGCTG CTGCAGTGGA AGGACCGTTT CAAAGAGAGC CTGAAGTATT TTGTGGGCGT TGATATCGGT GGCACCAACA CCCGTGTGGC GCTGGACACC GGTAGCAGCG ATGAGAGCTA CGTTCAGATC GCGAAATTCC GTGCGAGCAG CAGCAAGCAC ATTGTGGAAG GTCTGCAGCA AGTGGCGCGT CAAGTTGCGG ACATTCTGGG TGTTCCGGCG AGCGGTGCGT GCCTGGCGGG TGCGGGTCGT ATCAGCGATG ATGGCCTGAG CCTGGATATT ACCAACTATC CGGGTACCCC GGCGGACCGT ACCCTGACCA GCGATCAACT GCCGACCTAC CTGTTCCCGG CGGATGCGAC CCACTTTATC AACGATCTGG AGAGCACCTG CTATGGCCTG AAAAGCCTGA ACGAAAGCCA GCAACTGAGC AGCTTCTTTA AGCCGCTGTG GAGCACCAGC GACACCGTGA TGCCGATGGA GTTTGGTCAT GCGCTGTACA GCCCGGCGAC CGATCCGAGC AAGAAAGACG AGGAAGATCG TCTGGCGGCG TACCTGAGCA AAACCCTGTA TAGCGGCAAG CACGCGATCG AGTACGAAGA CATTGTGAGC GGTCGTGGCG TGCTGGCGGT TTATCAATGG ATCACCGCGG AGCACAAAGA AGCGGCGAAG TACGAGAGCG CGGAGGAAGT TAGCACCGCG GCGTTCCGTG AAGATCCGTG CCCGTTTGCG ACCAAAGCGC TGCTGATTCA CTATCGTTTC CTGATGCGTG TGGCGAAAAA CCTGTGCGTT GGTCTGCAGG CGAAGGGCAT GTTTCTGGCG GGTGACAACC AAGTGGTTAA CAACCCGTTC TTTGAAAAAC ACCTGCCGGA GATGCGTGCG GAGTTCCTGG ATCACCCGAA GCCGGACTGG ATCGATATGG TTGAGCTGTA CACCCAGACC GAGAGCTTCA ACATTAACCT GCACGGCGCG CTGTACTATG CGCGTACCGA TCAACGTTGA TAAGCTTGCG GCCGCACTCG AGCACCACCA CCACCACCAC TGAGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGAT