AccaA.00032.a
S-adenosyl-L-homocysteine hydrolase
| CENTER ID: | AccaA.00032.a |
| ORGANISM: | Acanthamoeba castellanii |
| ASSOCIATED DISEASE: | Amoebic keratitis and encephalomyelitis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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You may order multiple materials at a time at no cost to you, as this contract is funded
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| AccaA.00032.a.B1.GE43119 | full length | 1 | 477 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| AccaA.00032.a.B1.PW38644 | full length | 1 | 477 | ||
| AccaA.00032.a.B1.PW38645 | full length | 1 | 477 |
Structures
6UK3
DEPOSITED: 10/4/2019
DETERMINATION: XRay
CLONE: AccaA.00032.a.B1.GE43119
PROTEIN: AccaA.00032.a.B1.PW38645
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| EuPathDB: | AmoebaDB:ACA1_054200 |
| UniProt: | L8H6B5 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQTQTQTQPA GNAFQYEIRD ISLAEFGRKE INLAEHEMPG LMQTREKYGA EQPLKGVRLA GSLHMTIQTA VLIETLQALG ANVRWCSCNI FSTQDHAAAA IVAAGTPVFA WKGETLEEYW ECTWKTLLFP DDMGPQLIVD DGGDATLMVH RGFYAEDNPS ILDDDEGSEE LAIVNKLLKR IQKEKPGYWH KIVPELKGVS EETTTGVHRL YEMMKEGKLL FPALNVNDSV TKSKFDNVYG CRHSLVDAIM RATDVMLSGK VACVLGYGDV GKGSAESLKG QGARVVVTEV DPICALQACM AGYEVVRIED VLDKAEIFVT TTGNCDIIRI EHMEKMRHNA IVCNIGHFDN EIQVKALKEF PGIKRIEIKP QVDQFVFPDG HAIVLLAEGR LVNLGCATGH PSFVMSNSFT NQTLAQISLW KEKYELGVYT LPKKLDEEVA RLHLEKLGAK LTVLTDKQAK YLGIAKDGPY KPDHYRY
NT Sequence
atgcagaccc aaactcagac ccagcccgct ggcaatgcct tccagtacga aatcagggac atcagccttg ctgagttcgg tcgcaaggag atcaacctcg ctgagcacga gatgcccggt ctcatgcaga cgcgcgagaa gtacggcgct gagcagcccc tcaagggagt caggctcgcc ggttcgctcc acatgaccat ccagaccgcc gtcctcatcg agaccctcca ggccctcggt gccaacgtcc gttggtgctc gtgcaacatc ttctcgaccc aggaccacgc cgccgccgcc atcgtggccg ccggtacccc cgtcttcgcc tggaagggtg agaccctcga ggagtactgg gagtgcacct ggaagaccct cctcttcccc gacgacatgg gtccccagct catcgtcgac gatggtggtg atgccaccct catggtccac cgcggtttct acgctgagga caacccctcc attcttgatg acgacgaggg ttctgaggag ctcgccatcg tcaacaagct cctgaagcgc atccagaagg agaagcccgg ttactggcac aagatcgtcc ccgagctcaa gggtgtctcg gaggagacca ccaccggtgt ccaccgtctc tacgagatga tgaaggaggg caagctcctt ttccccgccc tcaacgtcaa cgactccgtg accaagtcca agttcgacaa cgtctacggt tgccgtcact cgctcgtcga cgccatcatg cgcgccaccg acgtgatgct ctccggcaag gtcgcctgcg tgctcggcta cggtgacgtc ggcaagggtt ccgctgagtc cctcaagggc cagggtgctc gcgtcgtcgt gactgaggtc gatcccatct gcgccctcca ggcctgcatg gctggctatg aggtggtccg cattgaggac gtgctcgaca aggccgagat cttcgtcacc accaccggca actgcgatat catccgcatt gagcacatgg agaagatgcg ccacaacgcc atcgtgtgca acatcggtca cttcgacaat gagatccagg tgaaggccct caaggagttc cccggtatca agaggatcga gatcaagcct caggtcgacc agttcgtctt ccccgacggc catgccatcg tcctcctcgc tgagggccgc ctcgtcaact tgggctgcgc caccggccac ccttcgttcg tgatgtcgaa ctcgttcacc aaccagaccc ttgctcagat ctccctctgg aaggagaagt acgagctggg cgtgtacacg ctccccaaga agctcgacga ggaagtcgct cgcctccacc tcgagaagct cggcgccaag ctcaccgtcc tcaccgacaa gcaggccaag tacctcggca tcgccaagga cggaccctac aagcccgacc actaccgcta ctaa
Details for AccaA.00032.a.B1.GE43119
| HARVESTED ON: | 5/17/2019 |
| SEQUENCED ON: | 5/24/2019 |
| EXPECTED MW: | 54kDa |
| OBSERVED MW: | 54kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMQ TQTQTQPAGN AFQYEIRDIS LAEFGRKEIN LAEHEMPGLM QTREKYGAEQ PLKGVRLAGS LHMTIQTAVL IETLQALGAN VRWCSCNIFS TQDHAAAAIV AAGTPVFAWK GETLEEYWEC TWKTLLFPDD MGPQLIVDDG GDATLMVHRG FYAEDNPSIL DDDEGSEELA IVNKLLKRIQ KEKPGYWHKI VPELKGVSEE TTTGVHRLYE MMKXXKLLFP ALNVNDSVTK SKFDNVYGCR HSLVDAIMRA TDVMLSGKVA CVLGYGDVGK GSAESLKGQG ARVVVTEVDP ICALQACMAG YEVVRIEDVL DKAEIFVTTT GNCDIIRIEH MEKMRHNAIV CNIGHFDNEI QVKALKEFPG IKRIEIKPQV DQFVFPDGHA IVLLAEGRLV NLGCATGHPS FVMSNSFTNQ TLAQISLWKE KYELGVYTLP KKLDEEVARL HLEKLGAKLT VLTDKQAKYL GIAKDGPXKP DHYRY
Validated NT Sequence
atggctcacc accaccacca ccatatgcag acccaaactc agacccagcc cgctggcaat gccttccagt acgaaatcag ggacatcagc cttgctgagt tcggtcgcaa ggagatcaac ctcgctgagc acgagatgcc cggtctcatg cagacgcgcg agaagtacgg cgctgagcag cccctcaagg gagtcaggct cgccggttcg ctccacatga ccatccagac cgccgtcctc atcgagaccc tccaggccct cggtgccaac gtccgttggt gctcgtgcaa catcttctcg acccaggacc acgccgccgc cgccatcgtg gccgccggta cccccgtctt cgcctggaag ggtgagaccc tcgaggagta ctgggagtgc acctggaaga ccctcctctt ccccgacgac atgggtcccc agctcatcgt cgacgatggt ggt-gatgcc accctcatgg tccac-cgcg gtttctacgc tgaggacaac ccctccattc ttgatgacga cgagggttct gaggagctcg ccatcgtcaa caagctcctg aagcgcatcc agaaggagaa gcccggttac tggcacaaga tcgtccccga gctcaagggt gtctcggagg agaccaccac cggtgtccac cgtctctacg agatgatgaa ggangncaag ctccttttcc ccgccctcaa cgtcaacgac tccgtgacca agtccaagtt cgacaacgtc tacggttgcc gtcactcgct cgtcgacgcc atcatgcgcg ccaccgacgt gatgctctcc ggcaaggtcg cctgcgtgct cggctacggt gacgtcggca agggttccgc tgagtccctc aagggccagg gtgctcgcgt cgtcgtgact gaggtcgatc ccatctgcgc cctccaggcc tgcatggctg gctatgaggt ggtccgcatt gaggacgtgc tcgacaaggc cgagatcttc gtcaccacca ccggcaactg cgatatcatc cgcattgagc acatggagaa gatgcgccac aacgccatcg tgtgcaacat cggtcacttc gacaatgaga tccaggtgaa ggccctcaag gagttccccg gtatcaagag gatcgagatc aagcctcagg tcgaccagtt cgtcttcccc gacggccatg ccatcgtcct cctcgctgag ggccgcctcg tcaacttggg ctgcgccacc ggccaccctt cgttcgtgat gtcgaactcg ttcaccaacc agacccttgc tcagatctcc ctctggaagg agaagtacga gctgggcgtg tacacgctcc ccaagaagct cgacgaggaa gtcgctcgcc tccacctcga gaagctcggc gccaagctca ccgtcctcac cgacaagcag gccaagtacc tcggcatcgc caaggacgga cccnacaagc ccgaccacta ccgctactaa
Expected Protein Sequence
MAHHHHHHMQ TQTQTQPAGN AFQYEIRDIS LAEFGRKEIN LAEHEMPGLM QTREKYGAEQ PLKGVRLAGS LHMTIQTAVL IETLQALGAN VRWCSCNIFS TQDHAAAAIV AAGTPVFAWK GETLEEYWEC TWKTLLFPDD MGPQLIVDDG GDATLMVHRG FYAEDNPSIL DDDEGSEELA IVNKLLKRIQ KEKPGYWHKI VPELKGVSEE TTTGVHRLYE MMKEGKLLFP ALNVNDSVTK SKFDNVYGCR HSLVDAIMRA TDVMLSGKVA CVLGYGDVGK GSAESLKGQG ARVVVTEVDP ICALQACMAG YEVVRIEDVL DKAEIFVTTT GNCDIIRIEH MEKMRHNAIV CNIGHFDNEI QVKALKEFPG IKRIEIKPQV DQFVFPDGHA IVLLAEGRLV NLGCATGHPS FVMSNSFTNQ TLAQISLWKE KYELGVYTLP KKLDEEVARL HLEKLGAKLT VLTDKQAKYL GIAKDGPYKP DHYRY
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcaga cccaaactca gacccagccc gctggcaatg ccttccagta cgaaatcagg gacatcagcc ttgctgagtt cggtcgcaag gagatcaacc tcgctgagca cgagatgccc ggtctcatgc agacgcgcga gaagtacggc gctgagcagc ccctcaaggg agtcaggctc gccggttcgc tccacatgac catccagacc gccgtcctca tcgagaccct ccaggccctc ggtgccaacg tccgttggtg ctcgtgcaac atcttctcga cccaggacca cgccgccgcc gccatcgtgg ccgccggtac ccccgtcttc gcctggaagg gtgagaccct cgaggagtac tgggagtgca cctggaagac cctcctcttc cccgacgaca tgggtcccca gctcatcgtc gacgatggtg gtgatgccac cctcatggtc caccgcggtt tctacgctga ggacaacccc tccattcttg atgacgacga gggttctgag gagctcgcca tcgtcaacaa gctcctgaag cgcatccaga aggagaagcc cggttactgg cacaagatcg tccccgagct caagggtgtc tcggaggaga ccaccaccgg tgtccaccgt ctctacgaga tgatgaagga gggcaagctc cttttccccg ccctcaacgt caacgactcc gtgaccaagt ccaagttcga caacgtctac ggttgccgtc actcgctcgt cgacgccatc atgcgcgcca ccgacgtgat gctctccggc aaggtcgcct gcgtgctcgg ctacggtgac gtcggcaagg gttccgctga gtccctcaag ggccagggtg ctcgcgtcgt cgtgactgag gtcgatccca tctgcgccct ccaggcctgc atggctggct atgaggtggt ccgcattgag gacgtgctcg acaaggccga gatcttcgtc accaccaccg gcaactgcga tatcatccgc attgagcaca tggagaagat gcgccacaac gccatcgtgt gcaacatcgg tcacttcgac aatgagatcc aggtgaaggc cctcaaggag ttccccggta tcaagaggat cgagatcaag cctcaggtcg accagttcgt cttccccgac ggccatgcca tcgtcctcct cgctgagggc cgcctcgtca acttgggctg cgccaccggc cacccttcgt tcgtgatgtc gaactcgttc accaaccaga cccttgctca gatctccctc tggaaggaga agtacgagct gggcgtgtac acgctcccca agaagctcga cgaggaagtc gctcgcctcc acctcgagaa gctcggcgcc aagctcaccg tcctcaccga caagcaggcc aagtacctcg gcatcgccaa ggacggaccc tacaagcccg accactaccg ctactgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for AccaA.00032.a.B1.PW38644
| PURIFICATION DATe: | 6/18/2019 |
| CONCENTRATION: | 100mg/ml |
| OBSERVED MW: | 52kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 1 |
| VIAL VOLUME: | 55µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMQ TQTQTQPAGN AFQYEIRDIS LAEFGRKEIN LAEHEMPGLM QTREKYGAEQ PLKGVRLAGS LHMTIQTAVL IETLQALGAN VRWCSCNIFS TQDHAAAAIV AAGTPVFAWK GETLEEYWEC TWKTLLFPDD MGPQLIVDDG GDATLMVHRG FYAEDNPSIL DDDEGSEELA IVNKLLKRIQ KEKPGYWHKI VPELKGVSEE TTTGVHRLYE MMKXXKLLFP ALNVNDSVTK SKFDNVYGCR HSLVDAIMRA TDVMLSGKVA CVLGYGDVGK GSAESLKGQG ARVVVTEVDP ICALQACMAG YEVVRIEDVL DKAEIFVTTT GNCDIIRIEH MEKMRHNAIV CNIGHFDNEI QVKALKEFPG IKRIEIKPQV DQFVFPDGHA IVLLAEGRLV NLGCATGHPS FVMSNSFTNQ TLAQISLWKE KYELGVYTLP KKLDEEVARL HLEKLGAKLT VLTDKQAKYL GIAKDGPXKP DHYRY
Validated NT Sequence
atggctcacc accaccacca ccatatgcag acccaaactc agacccagcc cgctggcaat gccttccagt acgaaatcag ggacatcagc cttgctgagt tcggtcgcaa ggagatcaac ctcgctgagc acgagatgcc cggtctcatg cagacgcgcg agaagtacgg cgctgagcag cccctcaagg gagtcaggct cgccggttcg ctccacatga ccatccagac cgccgtcctc atcgagaccc tccaggccct cggtgccaac gtccgttggt gctcgtgcaa catcttctcg acccaggacc acgccgccgc cgccatcgtg gccgccggta cccccgtctt cgcctggaag ggtgagaccc tcgaggagta ctgggagtgc acctggaaga ccctcctctt ccccgacgac atgggtcccc agctcatcgt cgacgatggt ggt-gatgcc accctcatgg tccac-cgcg gtttctacgc tgaggacaac ccctccattc ttgatgacga cgagggttct gaggagctcg ccatcgtcaa caagctcctg aagcgcatcc agaaggagaa gcccggttac tggcacaaga tcgtccccga gctcaagggt gtctcggagg agaccaccac cggtgtccac cgtctctacg agatgatgaa ggangncaag ctccttttcc ccgccctcaa cgtcaacgac tccgtgacca agtccaagtt cgacaacgtc tacggttgcc gtcactcgct cgtcgacgcc atcatgcgcg ccaccgacgt gatgctctcc ggcaaggtcg cctgcgtgct cggctacggt gacgtcggca agggttccgc tgagtccctc aagggccagg gtgctcgcgt cgtcgtgact gaggtcgatc ccatctgcgc cctccaggcc tgcatggctg gctatgaggt ggtccgcatt gaggacgtgc tcgacaaggc cgagatcttc gtcaccacca ccggcaactg cgatatcatc cgcattgagc acatggagaa gatgcgccac aacgccatcg tgtgcaacat cggtcacttc gacaatgaga tccaggtgaa ggccctcaag gagttccccg gtatcaagag gatcgagatc aagcctcagg tcgaccagtt cgtcttcccc gacggccatg ccatcgtcct cctcgctgag ggccgcctcg tcaacttggg ctgcgccacc ggccaccctt cgttcgtgat gtcgaactcg ttcaccaacc agacccttgc tcagatctcc ctctggaagg agaagtacga gctgggcgtg tacacgctcc ccaagaagct cgacgaggaa gtcgctcgcc tccacctcga gaagctcggc gccaagctca ccgtcctcac cgacaagcag gccaagtacc tcggcatcgc caaggacgga cccnacaagc ccgaccacta ccgctactaa
Expressed Protein Sequence
MAHHHHHHMQ TQTQTQPAGN AFQYEIRDIS LAEFGRKEIN LAEHEMPGLM QTREKYGAEQ PLKGVRLAGS LHMTIQTAVL IETLQALGAN VRWCSCNIFS TQDHAAAAIV AAGTPVFAWK GETLEEYWEC TWKTLLFPDD MGPQLIVDDG GDATLMVHRG FYAEDNPSIL DDDEGSEELA IVNKLLKRIQ KEKPGYWHKI VPELKGVSEE TTTGVHRLYE MMKEGKLLFP ALNVNDSVTK SKFDNVYGCR HSLVDAIMRA TDVMLSGKVA CVLGYGDVGK GSAESLKGQG ARVVVTEVDP ICALQACMAG YEVVRIEDVL DKAEIFVTTT GNCDIIRIEH MEKMRHNAIV CNIGHFDNEI QVKALKEFPG IKRIEIKPQV DQFVFPDGHA IVLLAEGRLV NLGCATGHPS FVMSNSFTNQ TLAQISLWKE KYELGVYTLP KKLDEEVARL HLEKLGAKLT VLTDKQAKYL GIAKDGPYKP DHYRY
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCAGA CCCAAACTCA GACCCAGCCC GCTGGCAATG CCTTCCAGTA CGAAATCAGG GACATCAGCC TTGCTGAGTT CGGTCGCAAG GAGATCAACC TCGCTGAGCA CGAGATGCCC GGTCTCATGC AGACGCGCGA GAAGTACGGC GCTGAGCAGC CCCTCAAGGG AGTCAGGCTC GCCGGTTCGC TCCACATGAC CATCCAGACC GCCGTCCTCA TCGAGACCCT CCAGGCCCTC GGTGCCAACG TCCGTTGGTG CTCGTGCAAC ATCTTCTCGA CCCAGGACCA CGCCGCCGCC GCCATCGTGG CCGCCGGTAC CCCCGTCTTC GCCTGGAAGG GTGAGACCCT CGAGGAGTAC TGGGAGTGCA CCTGGAAGAC CCTCCTCTTC CCCGACGACA TGGGTCCCCA GCTCATCGTC GACGATGGTG GTGATGCCAC CCTCATGGTC CACCGCGGTT TCTACGCTGA GGACAACCCC TCCATTCTTG ATGACGACGA GGGTTCTGAG GAGCTCGCCA TCGTCAACAA GCTCCTGAAG CGCATCCAGA AGGAGAAGCC CGGTTACTGG CACAAGATCG TCCCCGAGCT CAAGGGTGTC TCGGAGGAGA CCACCACCGG TGTCCACCGT CTCTACGAGA TGATGAAGGA GGGCAAGCTC CTTTTCCCCG CCCTCAACGT CAACGACTCC GTGACCAAGT CCAAGTTCGA CAACGTCTAC GGTTGCCGTC ACTCGCTCGT CGACGCCATC ATGCGCGCCA CCGACGTGAT GCTCTCCGGC AAGGTCGCCT GCGTGCTCGG CTACGGTGAC GTCGGCAAGG GTTCCGCTGA GTCCCTCAAG GGCCAGGGTG CTCGCGTCGT CGTGACTGAG GTCGATCCCA TCTGCGCCCT CCAGGCCTGC ATGGCTGGCT ATGAGGTGGT CCGCATTGAG GACGTGCTCG ACAAGGCCGA GATCTTCGTC ACCACCACCG GCAACTGCGA TATCATCCGC ATTGAGCACA TGGAGAAGAT GCGCCACAAC GCCATCGTGT GCAACATCGG TCACTTCGAC AATGAGATCC AGGTGAAGGC CCTCAAGGAG TTCCCCGGTA TCAAGAGGAT CGAGATCAAG CCTCAGGTCG ACCAGTTCGT CTTCCCCGAC GGCCATGCCA TCGTCCTCCT CGCTGAGGGC CGCCTCGTCA ACTTGGGCTG CGCCACCGGC CACCCTTCGT TCGTGATGTC GAACTCGTTC ACCAACCAGA CCCTTGCTCA GATCTCCCTC TGGAAGGAGA AGTACGAGCT GGGCGTGTAC ACGCTCCCCA AGAAGCTCGA CGAGGAAGTC GCTCGCCTCC ACCTCGAGAA GCTCGGCGCC AAGCTCACCG TCCTCACCGA CAAGCAGGCC AAGTACCTCG GCATCGCCAA GGACGGACCC TACAAGCCCG ACCACTACCG CTACTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT
Details for AccaA.00032.a.B1.PW38645
| PURIFICATION DATe: | 6/17/2019 |
| CONCENTRATION: | 20.81mg/ml |
| OBSERVED MW: | 52kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 16 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMQ TQTQTQPAGN AFQYEIRDIS LAEFGRKEIN LAEHEMPGLM QTREKYGAEQ PLKGVRLAGS LHMTIQTAVL IETLQALGAN VRWCSCNIFS TQDHAAAAIV AAGTPVFAWK GETLEEYWEC TWKTLLFPDD MGPQLIVDDG GDATLMVHRG FYAEDNPSIL DDDEGSEELA IVNKLLKRIQ KEKPGYWHKI VPELKGVSEE TTTGVHRLYE MMKXXKLLFP ALNVNDSVTK SKFDNVYGCR HSLVDAIMRA TDVMLSGKVA CVLGYGDVGK GSAESLKGQG ARVVVTEVDP ICALQACMAG YEVVRIEDVL DKAEIFVTTT GNCDIIRIEH MEKMRHNAIV CNIGHFDNEI QVKALKEFPG IKRIEIKPQV DQFVFPDGHA IVLLAEGRLV NLGCATGHPS FVMSNSFTNQ TLAQISLWKE KYELGVYTLP KKLDEEVARL HLEKLGAKLT VLTDKQAKYL GIAKDGPXKP DHYRY
Validated NT Sequence
atggctcacc accaccacca ccatatgcag acccaaactc agacccagcc cgctggcaat gccttccagt acgaaatcag ggacatcagc cttgctgagt tcggtcgcaa ggagatcaac ctcgctgagc acgagatgcc cggtctcatg cagacgcgcg agaagtacgg cgctgagcag cccctcaagg gagtcaggct cgccggttcg ctccacatga ccatccagac cgccgtcctc atcgagaccc tccaggccct cggtgccaac gtccgttggt gctcgtgcaa catcttctcg acccaggacc acgccgccgc cgccatcgtg gccgccggta cccccgtctt cgcctggaag ggtgagaccc tcgaggagta ctgggagtgc acctggaaga ccctcctctt ccccgacgac atgggtcccc agctcatcgt cgacgatggt ggt-gatgcc accctcatgg tccac-cgcg gtttctacgc tgaggacaac ccctccattc ttgatgacga cgagggttct gaggagctcg ccatcgtcaa caagctcctg aagcgcatcc agaaggagaa gcccggttac tggcacaaga tcgtccccga gctcaagggt gtctcggagg agaccaccac cggtgtccac cgtctctacg agatgatgaa ggangncaag ctccttttcc ccgccctcaa cgtcaacgac tccgtgacca agtccaagtt cgacaacgtc tacggttgcc gtcactcgct cgtcgacgcc atcatgcgcg ccaccgacgt gatgctctcc ggcaaggtcg cctgcgtgct cggctacggt gacgtcggca agggttccgc tgagtccctc aagggccagg gtgctcgcgt cgtcgtgact gaggtcgatc ccatctgcgc cctccaggcc tgcatggctg gctatgaggt ggtccgcatt gaggacgtgc tcgacaaggc cgagatcttc gtcaccacca ccggcaactg cgatatcatc cgcattgagc acatggagaa gatgcgccac aacgccatcg tgtgcaacat cggtcacttc gacaatgaga tccaggtgaa ggccctcaag gagttccccg gtatcaagag gatcgagatc aagcctcagg tcgaccagtt cgtcttcccc gacggccatg ccatcgtcct cctcgctgag ggccgcctcg tcaacttggg ctgcgccacc ggccaccctt cgttcgtgat gtcgaactcg ttcaccaacc agacccttgc tcagatctcc ctctggaagg agaagtacga gctgggcgtg tacacgctcc ccaagaagct cgacgaggaa gtcgctcgcc tccacctcga gaagctcggc gccaagctca ccgtcctcac cgacaagcag gccaagtacc tcggcatcgc caaggacgga cccnacaagc ccgaccacta ccgctactaa
Expressed Protein Sequence
MAHHHHHHMQ TQTQTQPAGN AFQYEIRDIS LAEFGRKEIN LAEHEMPGLM QTREKYGAEQ PLKGVRLAGS LHMTIQTAVL IETLQALGAN VRWCSCNIFS TQDHAAAAIV AAGTPVFAWK GETLEEYWEC TWKTLLFPDD MGPQLIVDDG GDATLMVHRG FYAEDNPSIL DDDEGSEELA IVNKLLKRIQ KEKPGYWHKI VPELKGVSEE TTTGVHRLYE MMKEGKLLFP ALNVNDSVTK SKFDNVYGCR HSLVDAIMRA TDVMLSGKVA CVLGYGDVGK GSAESLKGQG ARVVVTEVDP ICALQACMAG YEVVRIEDVL DKAEIFVTTT GNCDIIRIEH MEKMRHNAIV CNIGHFDNEI QVKALKEFPG IKRIEIKPQV DQFVFPDGHA IVLLAEGRLV NLGCATGHPS FVMSNSFTNQ TLAQISLWKE KYELGVYTLP KKLDEEVARL HLEKLGAKLT VLTDKQAKYL GIAKDGPYKP DHYRY
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCAGA CCCAAACTCA GACCCAGCCC GCTGGCAATG CCTTCCAGTA CGAAATCAGG GACATCAGCC TTGCTGAGTT CGGTCGCAAG GAGATCAACC TCGCTGAGCA CGAGATGCCC GGTCTCATGC AGACGCGCGA GAAGTACGGC GCTGAGCAGC CCCTCAAGGG AGTCAGGCTC GCCGGTTCGC TCCACATGAC CATCCAGACC GCCGTCCTCA TCGAGACCCT CCAGGCCCTC GGTGCCAACG TCCGTTGGTG CTCGTGCAAC ATCTTCTCGA CCCAGGACCA CGCCGCCGCC GCCATCGTGG CCGCCGGTAC CCCCGTCTTC GCCTGGAAGG GTGAGACCCT CGAGGAGTAC TGGGAGTGCA CCTGGAAGAC CCTCCTCTTC CCCGACGACA TGGGTCCCCA GCTCATCGTC GACGATGGTG GTGATGCCAC CCTCATGGTC CACCGCGGTT TCTACGCTGA GGACAACCCC TCCATTCTTG ATGACGACGA GGGTTCTGAG GAGCTCGCCA TCGTCAACAA GCTCCTGAAG CGCATCCAGA AGGAGAAGCC CGGTTACTGG CACAAGATCG TCCCCGAGCT CAAGGGTGTC TCGGAGGAGA CCACCACCGG TGTCCACCGT CTCTACGAGA TGATGAAGGA GGGCAAGCTC CTTTTCCCCG CCCTCAACGT CAACGACTCC GTGACCAAGT CCAAGTTCGA CAACGTCTAC GGTTGCCGTC ACTCGCTCGT CGACGCCATC ATGCGCGCCA CCGACGTGAT GCTCTCCGGC AAGGTCGCCT GCGTGCTCGG CTACGGTGAC GTCGGCAAGG GTTCCGCTGA GTCCCTCAAG GGCCAGGGTG CTCGCGTCGT CGTGACTGAG GTCGATCCCA TCTGCGCCCT CCAGGCCTGC ATGGCTGGCT ATGAGGTGGT CCGCATTGAG GACGTGCTCG ACAAGGCCGA GATCTTCGTC ACCACCACCG GCAACTGCGA TATCATCCGC ATTGAGCACA TGGAGAAGAT GCGCCACAAC GCCATCGTGT GCAACATCGG TCACTTCGAC AATGAGATCC AGGTGAAGGC CCTCAAGGAG TTCCCCGGTA TCAAGAGGAT CGAGATCAAG CCTCAGGTCG ACCAGTTCGT CTTCCCCGAC GGCCATGCCA TCGTCCTCCT CGCTGAGGGC CGCCTCGTCA ACTTGGGCTG CGCCACCGGC CACCCTTCGT TCGTGATGTC GAACTCGTTC ACCAACCAGA CCCTTGCTCA GATCTCCCTC TGGAAGGAGA AGTACGAGCT GGGCGTGTAC ACGCTCCCCA AGAAGCTCGA CGAGGAAGTC GCTCGCCTCC ACCTCGAGAA GCTCGGCGCC AAGCTCACCG TCCTCACCGA CAAGCAGGCC AAGTACCTCG GCATCGCCAA GGACGGACCC TACAAGCCCG ACCACTACCG CTACTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT