AcbyA.19027.a

Putative transcriptional regulator (AraC family) (AdiY)

CENTER ID: AcbyA.19027.a
ORGANISM: Acinetobacter baylyi ATCC 33305 / BD413 / ADP1
ASSOCIATED DISEASE:
CURRENT STATUS: cloned
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AcbyA.19027.a.B4.GE42195 arabinose-binding domain 19 213
AcbyA.19027.a.B3.GE42194 HTH-type 243 348
AcbyA.19027.a.B2.GE42193 ordered domain 18 348
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
UniProt: Q6F8C0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQVNIKETDG LNVNRDTISI HFVNAALTGV KRLGMDVETL LSHVGIEAEL LRQPKARISP EQYTRFIKML WMVTQDEHVG FDIQPRRLGT FAIMCQLIIH AKTLGQALEL SSQFYKLFGD EWSVTLERDK HEARLVPLIP KTLDPDHFIT ESMLMIWHGL ASWLIERRLP LERVHFSYPR PAHADEYDAL FFAPVMQFDA PRTEITFAAD YLDLPLRQDE QTLEEFLKAA PAQLLIKFKN TNSLTSRIRE VLKSQIGEEM PTLNDVASML YLSPQTLRRR LAAEGKSYQG VKDALRRDAA IHLLLNPDLT LEDVAQQVGF SETSTFHRAF KKWTGVTPGL YRQLHGYH
NT Sequence
ctgcaagtga acataaaaga gactgacgga ttaaacgtaa accgcgatac gattagtatt catttcgtca acgcggcttt aactggcgtc aaacgccttg gaatggatgt cgaaacctta ctgtctcatg ttggaattga ggcagaacta ctacgtcaac ctaaagctcg tatttcacca gaacaatata ctcgttttat taaaatgctg tggatggtga ctcaggatga gcacgtcggc tttgacatac agccacgtcg cctgggtaca tttgccatta tgtgccaatt aattattcat gccaaaacac ttggacaagc attagaactg tcttcacaat tttacaagct gtttggtgat gagtggtctg tgaccttaga acgtgacaag catgaagctc gtttggtccc acttattcct aaaacacttg atccagatca ctttattaca gaaagcatgc tcatgatctg gcatggattg gcgtcatggc tgattgaacg tcgcttgcct ctggaacgtg tacactttag ttacccacgt cctgcacatg cagatgagta tgatgcttta ttttttgcac ccgttatgca atttgatgca ccacgtaccg agatcacgtt cgctgcggat tatttagatc tgcctcttcg ccaggatgaa cagacactag aagaattttt aaaggccgca cctgcacagt tattaatcaa atttaaaaat accaactcgc tgacctcacg tattcgtgaa gttttaaaaa gccagattgg tgaagaaatg ccgaccctca atgatgttgc atctatgctg tatctgtcac cacaaacgct acgtcgtcgc cttgctgccg aaggcaagag ctatcaaggc gttaaagatg cattacgtcg tgatgccgca attcacctgt tgctcaaccc agatttgacg cttgaagatg ttgcacagca ggtcggcttt agtgaaacca gtactttcca tcgtgcattt aaaaaatgga ctggcgtcac accgggttta tatcgtcaat tacacggtta tcattaa
Details for AcbyA.19027.a.B4.GE42195
HARVESTED ON: data unavailable
SEQUENCED ON: 3/1/2018
EXPECTED MW: 24kDa
OBSERVED MW: 19kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMS IHFVNAALTG VKRLGMDVET LLSHVGIEAE LLRQPKARIS PEQYTRFIKM LWMVTQDEHV GFDIQPRRLG TFAIMCQLII HAKTLGQALE LSSQFYKLFG DEWSVTLERD KHEARLVPLI PKTLDPDHFI TESMLMIWHG LASWLIERRL PLERVHFSYP RPAHADEYDA LFFAPVMQFD APRTEITFAA DYLD
Validated NT Sequence
atcttactca cttaatctaa ataatccgca gcgaacgtga tctcggtacg tggtgcatca aattgcataa cgggtgcaaa aaataaagca tcatactcat ctgcatgtgc aggacgtggg taactaaagt gtacacgttc cagaggcaag cgacgttcaa tcagccatga cgccaatcca tgccagatca tgagcatgct ttctgtaata aagtgatctg gatcaagtgt tttaggaata agtgggacca aacgagcttc atgcttgtca cgttctaagg tcacagacca ctcatcacca aacagcttgt aaaattgtga agacagttct aatgcttgtc caagtgtttt ggcatgaata attaattggc acataatggc aaatgtaccc aggcgacgtg gctgtatgtc aaagccgacg tgctcatcct gagtcaccat ccacagcatt ttaataaaac gagtatattg ttctggtgaa atacgagctt taggttgacg tagtagttct gcctcaattc caacatgaga cagtaaggtt tcgacatcca ttccaaggcg tttgacgcca gttaaagccg cgttgacgaa atgaatactc atatggtggt ggtggtggtg agccat
Expected Protein Sequence
MAHHHHHHSI HFVNAALTGV KRLGMDVETL LSHVGIEAEL LRQPKARISP EQYTRFIKML WMVTQDEHVG FDIQPRRLGT FAIMCQLIIH AKTLGQALEL SSQFYKLFGD EWSVTLERDK HEARLVPLIP KTLDPDHFIT ESMLMIWHGL ASWLIERRLP LERVHFSYPR PAHADEYDAL FFAPVMQFDA PRTEITFAAD YLD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catagtattc atttcgtcaa cgcggcttta actggcgtca aacgccttgg aatggatgtc gaaaccttac tgtctcatgt tggaattgag gcagaactac tacgtcaacc taaagctcgt atttcaccag aacaatatac tcgttttatt aaaatgctgt ggatggtgac tcaggatgag cacgtcggct ttgacataca gccacgtcgc ctgggtacat ttgccattat gtgccaatta attattcatg ccaaaacact tggacaagca ttagaactgt cttcacaatt ttacaagctg tttggtgatg agtggtctgt gaccttagaa cgtgacaagc atgaagctcg tttggtccca cttattccta aaacacttga tccagatcac tttattacag aaagcatgct catgatctgg catggattgg cgtcatggct gattgaacgt cgcttgcctc tggaacgtgt acactttagt tacccacgtc ctgcacatgc agatgagtat gatgctttat tttttgcacc cgttatgcaa tttgatgcac cacgtaccga gatcacgttc gctgcggatt atttagattg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for AcbyA.19027.a.B3.GE42194
HARVESTED ON: data unavailable
SEQUENCED ON: 3/1/2018
EXPECTED MW: 13kDa
OBSERVED MW: 25kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMS LTSRIREVLK SQIGEEMPTL NDVASMLYLS PQTLRRRLAA EGKSYQGVKD ALRRDAAIHL LLNPDLTLED VAQQVGFSET STFHRAFKKW TGVTPGLYRQ LHGYH
Validated NT Sequence
atcttactca cttaatgata accgtgtaat tgacgatata aacccggtgt gacgccagtc cattttttaa atgcacgatg gaaagtactg gtttcactaa agccgacctg ctgtgcaaca tcttcaagcg tcaaatctgg gttgagcaac aggtgaattg cggcatcacg acgtaatgca tctttaacgc cttgatagct cttgccttcg gcagcaaggc gacgacgtag cgtttgtggt gacagataca gcatagatgc aacatcattg agggtcggca tttcttcacc aatctggctt tttaaaactt cacgaatacg tgaggtcagc gacatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHSL TSRIREVLKS QIGEEMPTLN DVASMLYLSP QTLRRRLAAE GKSYQGVKDA LRRDAAIHLL LNPDLTLEDV AQQVGFSETS TFHRAFKKWT GVTPGLYRQL HGYH
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattcgctga cctcacgtat tcgtgaagtt ttaaaaagcc agattggtga agaaatgccg accctcaatg atgttgcatc tatgctgtat ctgtcaccac aaacgctacg tcgtcgcctt gctgccgaag gcaagagcta tcaaggcgtt aaagatgcat tacgtcgtga tgccgcaatt cacctgttgc tcaacccaga tttgacgctt gaagatgttg cacagcaggt cggctttagt gaaaccagta ctttccatcg tgcatttaaa aaatggactg gcgtcacacc gggtttatat cgtcaattac acggttatca ttgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for AcbyA.19027.a.B2.GE42193
HARVESTED ON: data unavailable
SEQUENCED ON: 3/1/2018
EXPECTED MW: 39kDa
OBSERVED MW: 19kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMI SIHFVNAALT GVKRLGMDVE TLLSHVGIEA ELLRQPKARI SPEQYTRFIK MLWMVTQDEH VGFDIQPRRL GTFAIMCQLI IHAKTLGQAL ELSSQFYKLF GDEWSVTLER DKHEARLVPL IPKTLDPDHF ITESMLMIWH GLASWLIERR LPLERVHFSY PRPAHADEYD ALFFAPVMQF DAPRTEITFA ADYLDLPLRQ DEQTLEEFLK AAPAQLLIKF KNTNSLTSRI REVLKSQIGE EMPTLNDVAS MLYLSPQTLR RRLAAEGKSY QGVKDALRRD AAIHLLLNPD LTLEDVAQQV GFSETSTFHR AFKKWTGVTP GLXXXLHGYH
Validated NT Sequence
ttaatgataa ccgtgtaatn gnnnntataa acccggtgtg acgccagtcc attttttaaa tgcacgatgg aaagtactgg tttcactaaa gccgacctgc tgtgcaacat cttcaagcgt caaatctggg ttgagcaaca ggtgaattgc ggcatcacga cgtaatgcat ctttaacgcc ttgatagctc ttgccttcgg cagcaaggcg acgacgtagc gtttgtggtg acagatacag catagatgca acatcattga gggtcggcat ttcttcacca atctggcttt ttaaaacttc acgaatacgt gaggtcagcg agttggtatt tttaaatttg attaataact gtgcaggtgc ggcctttaaa aattcttcta gtgtctgttc atcctggcga agaggcagat ctaaataatc cgcagcgaac gtgatctcgg tacgtggtgc atcaaattgc ataacgggtg caaaaaataa agcatcatac tcatctgcat gtgcaggacg tgggtaacta aagtgtacac gttccagagg caagcgacgt tcaatcagcc atgacgccaa tccatgccag atcatgagca tgctttctgt aataaagtga tctggatcaa gtgttttagg aataagtggg accaaacgag cttcatgctt gtcacgttct aaggtcacag accactcatc accaaacagc ttgtaaaatt gtgaagacag ttctaatgct tgtccaagtg ttttggcatg aataattaat tggcacataa tggcaaatgt acccaggcga cgtggctgta tgtcaaagcc gacgtgctca tcctgagtca ccatccacag cattttaata aaacgagtat attgttctgg tgaaatacga gctttaggtt gacgtagtag ttctgcctca attccaacat gagacagtaa ggtttcgaca tccattccaa ggcgtttgac gccagttaaa gccgcgttga cgaaatgaat actaatcata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHIS IHFVNAALTG VKRLGMDVET LLSHVGIEAE LLRQPKARIS PEQYTRFIKM LWMVTQDEHV GFDIQPRRLG TFAIMCQLII HAKTLGQALE LSSQFYKLFG DEWSVTLERD KHEARLVPLI PKTLDPDHFI TESMLMIWHG LASWLIERRL PLERVHFSYP RPAHADEYDA LFFAPVMQFD APRTEITFAA DYLDLPLRQD EQTLEEFLKA APAQLLIKFK NTNSLTSRIR EVLKSQIGEE MPTLNDVASM LYLSPQTLRR RLAAEGKSYQ GVKDALRRDA AIHLLLNPDL TLEDVAQQVG FSETSTFHRA FKKWTGVTPG LYRQLHGYH
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catattagta ttcatttcgt caacgcggct ttaactggcg tcaaacgcct tggaatggat gtcgaaacct tactgtctca tgttggaatt gaggcagaac tactacgtca acctaaagct cgtatttcac cagaacaata tactcgtttt attaaaatgc tgtggatggt gactcaggat gagcacgtcg gctttgacat acagccacgt cgcctgggta catttgccat tatgtgccaa ttaattattc atgccaaaac acttggacaa gcattagaac tgtcttcaca attttacaag ctgtttggtg atgagtggtc tgtgacctta gaacgtgaca agcatgaagc tcgtttggtc ccacttattc ctaaaacact tgatccagat cactttatta cagaaagcat gctcatgatc tggcatggat tggcgtcatg gctgattgaa cgtcgcttgc ctctggaacg tgtacacttt agttacccac gtcctgcaca tgcagatgag tatgatgctt tattttttgc acccgttatg caatttgatg caccacgtac cgagatcacg ttcgctgcgg attatttaga tctgcctctt cgccaggatg aacagacact agaagaattt ttaaaggccg cacctgcaca gttattaatc aaatttaaaa ataccaactc gctgacctca cgtattcgtg aagttttaaa aagccagatt ggtgaagaaa tgccgaccct caatgatgtt gcatctatgc tgtatctgtc accacaaacg ctacgtcgtc gccttgctgc cgaaggcaag agctatcaag gcgttaaaga tgcattacgt cgtgatgccg caattcacct gttgctcaac ccagatttga cgcttgaaga tgttgcacag caggtcggct ttagtgaaac cagtactttc catcgtgcat ttaaaaaatg gactggcgtc acaccgggtt tatatcgtca attacacggt tatcattgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t