AcbaC.19028.a

hypothetical protein

CENTER ID: AcbaC.19028.a
ORGANISM: Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AcbaC.19028.a.B2.GE39046 DUF490 1149 1501
AcbaC.19028.a.B3.GE39047 UniRef90_A0A014FTU2 187 674
AcbaC.19028.a.B4.GE39048 middle chunk 675 1148
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|470.1345.peg.1546
GenBank: AKA31329.1
PGAT: acinetobacter:ABUW_1591
RefSeq: WP_000852508.1
UniProt: A0A0D5YH88

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAEVEQQPTS APSSPKKRRI LRSFLLTILI ILLLLVASII IMMSTDRGSR FLLDRVLQAQ QVIKYEYEGG NLLRGIILKN IIVQLKEVDV TLDRADVRLG WRSLILEKEV HLSNADVRNL VIINKAPPSD KPFEFKPIKL PFVLRVDVGD VDHLEIKNSG SVVNFHDVHL NDALWSETKL KFENSSMDMG YLSVHNATGN MDFSGKYPLN ATADLRIPSL KSLNIQNIKV AARGSLDTLQ AGVATTTPDL LTGWVVLHPV RHEVPMQGAL LLKNYHLPLL VEQKLFAKNG VIKFQGDIKQ LNLALETDLK GENLPEGQYN ALMNTDLVHQ LNITDFNGQV MKGAVNLKGL VNWKDHVTWD IKGRLDHVNP KDKAIPQVVQ DFLPPSLDAA VSSTGSLEKG TEVFANVDFD RYESWKLKLN QAPEKNKKPQ PMFMNVAWAN IDRAMPYIGW LSSDSGQVDL TLRDGQQDIK VSTKVYQHEQ TLLPAGQYLA TLNVKDNILN VPSFSFAAQK GSLTGQAKVL LPSEKRQLAW NALLNAKDFN PQSIHTAAPV NLLNGSIKAS GFAKPNQQII QLEKIDLKGR LAQAGQEMVA LAGKSTAALL FNDVKAGGGF KGFAVNYDGS LKALNQANGL LKFSIAGTPE FIRISQLQHD GVAGKIYATG ALNLKDRIAW DINSSLVRFK PQYFASSVKG EISGNVKTQG IWSDKLKRID IQQLNLAGYL NNKPVRGKGN LSILMDSNQK GFLPQQFEAN NLFLVYAQNQ LQATGNAQNL KIKLNAPALY DLYPGLSGRA YGDLSVQSQP RLKATANIAV DNFAFNTLVS IKKLRIQGEL PTSETTPTQL TAKLDNLRSG SRQIQSAEVN LTGTRKAHLL KVQGNNNVSK FYVQLAGGFN AKNDWLGQIQ KGSFDSRRIR LAQNQNAPVV FSSARSELYV GQHCWQSTNS QLCLDQPVRV SKAQGNISFV TQNLDLGDFA AFMPEGLAMT GQLNGYAKAS WVNGGHPKLD ARLVTRKGEL GLAAEDPQDP PTTLAYDELS VIAKSISEGL LFRVDVKTPD IGTGYANVII NPYQSSMPMH GEVAFNDVQL KVLKPFIQDV RSMSGTLALA GKVNGTLTQP QFTGEMRLKN GAISMISLPV NLTNVQVYSS IHQDMATIDG AFNSGQGVGL LKGSFDWKNA PRLQLNLKGD NLLVRQAPLI TAIANPNLTL DMYPFDKRLS LKGSVDVPRA RISMPETTAP VVNTSSDVRI VRQGQDPLAI LRAAKPWDIR ADISVNIGNQ VIFQGFNSNI PLVGRLNLTQ RGYETAMRAM GAIGVSQKVK IEAYGQSLDL NRAIARFNGP LANPTLDIDA NKNVQGSMVG VRVTGTASSP NIQVYNDAGL SEQEALNALV TGRINEGASG LSNAEGFKSD VNNTIAAAGI SMGLGGTRAL TNQIGRTFGL SGLALDAQGT GDDTQVSLTG YITPDLFIRY GVGVFTPVNK LTLRYQMNRR LYLEASQSLE RAIDLFYNWR F
NT Sequence
ATGGCGGAAG TAGAACAGCA GCCCACTTCG GCACCTAGCT CTCCTAAGAA GCGACGTATT TTGCGTAGCT TCTTGCTGAC AATCTTGATC ATACTTTTAT TACTTGTTGC TTCCATCATT ATTATGATGT CGACTGACCG TGGAAGTCGT TTTTTATTAG ATCGTGTTTT ACAAGCTCAG CAAGTCATTA AATACGAGTA TGAGGGGGGT AATTTACTCA GGGGAATTAT TCTTAAAAAT ATTATTGTTC AGTTAAAAGA AGTTGACGTT ACTTTAGATA GAGCTGATGT CCGTTTAGGG TGGCGTTCTT TAATATTAGA AAAAGAAGTG CATTTGAGCA ATGCAGATGT CCGTAACTTA GTTATTATTA ACAAAGCTCC GCCATCGGAT AAGCCATTTG AGTTTAAACC CATTAAATTA CCATTTGTGC TTCGTGTAGA TGTTGGAGAT GTTGATCATT TAGAAATTAA AAATTCAGGT TCTGTCGTAA ATTTCCATGA TGTTCATCTA AATGATGCTT TGTGGTCAGA GACAAAACTG AAATTTGAAA ATTCAAGTAT GGATATGGGG TATCTTTCTG TTCATAACGC AACAGGAAAT ATGGACTTTA GCGGCAAATA CCCACTGAAT GCGACTGCAG ATTTAAGAAT TCCTTCTTTA AAAAGTTTAA ACATCCAAAA TATTAAGGTG GCAGCGCGGG GTAGTTTAGA TACTTTGCAA GCCGGTGTAG CAACGACAAC ACCAGATTTA TTAACAGGTT GGGTTGTTTT ACATCCTGTT CGTCATGAAG TGCCTATGCA AGGTGCATTG TTACTCAAAA ATTATCACTT GCCTTTACTG GTTGAGCAAA AATTATTTGC TAAAAACGGT GTAATTAAAT TTCAGGGTGA TATTAAGCAA CTCAACTTAG CACTTGAGAC AGATTTAAAA GGGGAGAATT TACCTGAAGG CCAGTACAAC GCCTTAATGA ATACTGATCT GGTTCATCAG TTGAATATTA CTGATTTTAA CGGGCAGGTC ATGAAGGGTG CAGTTAATCT TAAAGGATTA GTGAACTGGA AAGATCATGT CACTTGGGAC ATCAAAGGTC GCCTAGATCA TGTTAATCCT AAAGACAAAG CTATTCCACA GGTCGTTCAG GATTTCTTGC CGCCAAGTCT AGATGCAGCC GTTTCTTCAA CTGGCTCTTT GGAAAAAGGT ACGGAAGTAT TTGCCAATGT AGACTTTGAC CGCTATGAAT CTTGGAAACT TAAACTAAAC CAAGCACCTG AAAAGAATAA GAAGCCACAA CCTATGTTTA TGAATGTGGC TTGGGCAAAT ATAGATCGTG CTATGCCATA TATAGGTTGG TTAAGTAGTG ATAGTGGTCA AGTTGATTTG ACTTTGCGAG ACGGTCAGCA AGATATTAAA GTTTCGACAA AGGTATATCA GCATGAACAG ACCTTATTAC CGGCTGGACA ATATTTAGCA ACTTTGAATG TTAAAGATAA TATTCTTAAT GTTCCTAGTT TTAGTTTTGC GGCTCAAAAG GGTAGCCTCA CAGGTCAGGC CAAAGTCTTA TTACCAAGTG AAAAACGTCA GCTGGCATGG AATGCATTAT TAAATGCAAA AGACTTTAAT CCACAAAGTA TACATACAGC GGCGCCTGTT AACTTGCTCA ACGGATCAAT TAAAGCCAGC GGTTTTGCAA AGCCAAATCA ACAAATTATT CAGCTTGAAA AAATTGATTT AAAAGGTCGC TTGGCTCAAG CAGGTCAAGA AATGGTTGCG CTAGCTGGAA AAAGTACAGC AGCTTTATTA TTTAATGATG TTAAAGCAGG TGGCGGTTTT AAAGGCTTTG CTGTCAATTA TGACGGATCT TTAAAAGCAC TTAATCAAGC CAATGGACTA TTAAAGTTTT CTATTGCAGG TACCCCAGAA TTTATCCGAA TTAGTCAGCT ACAACATGAT GGTGTTGCTG GGAAAATCTA TGCGACTGGG GCATTAAATT TAAAAGACCG TATTGCTTGG GATATTAATA GCTCGTTAGT ACGCTTTAAA CCTCAATATT TTGCCTCTAG TGTAAAAGGC GAAATTTCAG GAAATGTAAA AACACAAGGC ATCTGGTCTG ACAAGTTAAA GCGTATTGAT ATTCAACAAT TGAACCTTGC GGGTTATTTG AATAATAAGC CAGTCAGAGG TAAAGGTAAC TTATCCATAC TTATGGATTC GAACCAAAAA GGCTTTTTAC CTCAGCAATT TGAAGCAAAT AATCTATTTT TAGTTTATGC GCAAAACCAG TTGCAGGCGA CTGGTAATGC ACAGAATCTA AAAATTAAGC TGAATGCACC TGCGCTTTAT GATTTGTATC CGGGTTTAAG TGGGCGTGCA TATGGAGACT TGAGTGTCCA ATCTCAACCG CGTTTGAAAG CTACGGCGAA TATTGCGGTT GATAATTTTG CATTTAATAC ATTAGTCAGT ATTAAAAAAC TTCGTATCCA GGGTGAATTG CCTACTTCCG AAACAACACC AACTCAGTTA ACAGCAAAAT TAGACAATTT ACGTAGTGGT AGCCGTCAAA TTCAGTCTGC TGAGGTGAAT TTAACAGGAA CAAGAAAAGC ACATTTATTA AAAGTGCAAG GAAATAATAA TGTTTCTAAA TTCTATGTTC AGTTAGCAGG TGGATTTAAT GCGAAGAATG ATTGGTTAGG GCAAATTCAA AAAGGAAGTT TTGATTCACG CCGAATTCGT CTAGCACAAA ATCAAAATGC ACCAGTTGTT TTCTCATCGG CACGTTCTGA ATTATATGTT GGGCAACATT GCTGGCAAAG TACAAATAGC CAGTTGTGTC TTGACCAGCC TGTGCGTGTG AGTAAAGCAC AAGGTAATAT TTCATTTGTT ACTCAAAATC TAGATTTAGG TGATTTTGCT GCGTTCATGC CGGAAGGTTT GGCTATGACA GGCCAACTTA ATGGTTATGC TAAAGCTTCT TGGGTAAATG GTGGGCATCC TAAGCTTGAT GCACGCTTAG TAACCAGAAA AGGTGAACTG GGTCTAGCAG CGGAAGACCC GCAAGATCCA CCGACTACTT TGGCTTATGA TGAACTGAGT GTAATTGCAA AAAGTATTTC AGAAGGCTTA TTGTTCCGTG TCGATGTAAA AACACCTGAT ATTGGTACAG GATATGCGAA TGTTATTATT AATCCATACC AATCATCTAT GCCAATGCAT GGTGAGGTAG CTTTTAACGA TGTACAGTTA AAAGTGTTAA AACCATTCAT TCAAGATGTA CGTTCAATGA GTGGTACCTT AGCCTTAGCT GGCAAAGTGA ATGGTACTTT GACCCAGCCA CAGTTTACGG GCGAAATGCG TTTGAAAAAC GGGGCGATCA GTATGATTTC CCTGCCGGTT AATTTAACCA ATGTACAGGT GTATTCATCT ATTCATCAGG ACATGGCAAC AATTGATGGT GCATTCAATA GTGGACAAGG TGTAGGGTTA CTTAAAGGTA GTTTTGACTG GAAAAATGCA CCGCGTCTAC AGTTAAACTT AAAAGGTGAT AACTTACTTG TACGTCAAGC TCCATTAATT ACGGCCATTG CAAATCCGAA TTTGACACTT GATATGTATC CTTTTGATAA GCGTTTAAGC TTGAAAGGAT CAGTAGATGT TCCTCGTGCA CGTATTTCAA TGCCTGAAAC AACTGCACCA GTGGTTAACA CATCTTCAGA TGTTCGTATT GTTCGCCAAG GTCAGGACCC ACTTGCAATT TTACGTGCGG CAAAACCGTG GGATATCCGT GCAGATATTT CGGTAAATAT TGGCAATCAA GTCATCTTCC AAGGCTTTAA CAGTAATATT CCTTTAGTGG GGCGTTTGAA TTTAACCCAA CGCGGTTATG AAACAGCAAT GCGAGCAATG GGTGCTATTG GTGTAAGTCA AAAAGTGAAA ATTGAGGCCT ATGGTCAAAG CCTAGATTTA AACCGTGCAA TTGCCCGTTT TAATGGCCCA TTGGCAAACC CGACGTTAGA TATTGATGCG AATAAAAATG TTCAGGGTAG TATGGTTGGG GTTCGTGTCA CTGGTACTGC TTCATCACCA AACATTCAAG TTTATAATGA TGCAGGCCTT TCTGAACAAG AGGCATTAAA TGCACTCGTG ACAGGGCGTA TTAATGAAGG TGCCAGTGGT TTAAGTAATG CAGAAGGCTT TAAATCTGAT GTGAACAATA CAATTGCTGC TGCCGGTATT AGTATGGGCT TGGGTGGTAC TCGTGCTTTA ACGAATCAAA TTGGTCGTAC TTTTGGTTTG AGTGGTCTTG CATTAGATGC TCAAGGTACA GGAGATGATA CTCAAGTAAG TTTAACTGGT TACATTACAC CTGATTTATT TATTCGTTAT GGTGTGGGTG TGTTTACTCC AGTCAATAAA CTGACGTTGC GTTATCAAAT GAACCGCCGT TTGTATTTGG AAGCAAGTCA ATCTTTAGAA CGAGCAATAG ATTTATTCTA CAATTGGCGA TTCTAA
Details for AcbaC.19028.a.B2.GE39046
HARVESTED ON: 3/31/2015
SEQUENCED ON: 3/27/2015
EXPECTED MW: 39kDa
OBSERVED MW: 39kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMD GAFNSGQGVG LLKGSFDWKN APRLQLNLKG DNLLVRQAPL ITAIANPNLT LDMYPFDKRL SLKGSVDVPR ARISMPETTA PVVNTSSDVR IVRQGQDPLA ILRAAKPWDI RADISVNIGN QVIFQGFNSN IPLVGRLNLT QRGYETAMRA MGAIGVSQKV KIEAYGQSLD LNRAIARFNG PLANPTLDID ANKNVQGSMV GVRVTGTASS PNIQVYNDAG LSEQEALNAL VTGRINEGAS GLSNAEGFKS DVNNTIAAAG ISMGLGGTRA LTNQIGRTFG LSGLALDAQG TGDDTQVSLT GYITPDLFIR YGVGVFTPVN KLTLRYQMNR RLYLEASQSL ERAID
Validated NT Sequence
aatctattgc tcgttctaaa gattgacttg cttccaaata caaacggcgg ttcatttgat aacgcaacgt cagtttattg actggagtaa acacacccac accataacga ataaataaat caggtgtaat gtaaccagtt aaacttactt gagtatcatc tcctgtacct tgagcatcta atgcaagacc actcaaacca aaagtacgac caatttgatt cgttaaagca cgagtaccac ccaagcccat actaataccg gcagcagcaa ttgtattgtt cacatcagat ttaaagcctt ctgcattact taaaccactg gcaccttcat taatacgccc tgtcacgagt gcatttaatg cctcttgttc agaaaggcct gcatcattat aaacttgaat gtttggtgat gaagcagtac cagtgacacg aaccccaacc atactaccct gaacattttt attcgcatca atatctaacg tcgggtttgc caatgggcca ttaaaacggg caattgcacg gtttaaatct aggctttgac cataggcctc aattttcact ttttgactta caccaatagc acccattgct cgcattgctg tttcataacc gcgttgggtt aaattcaaac gccccactaa aggaatatta ctgttaaagc cttggaagat gacttgattg ccaatattta ccgaaatatc tgcacggata tcccacggtt ttgccgcacg taaaattgca agtgggtcct gaccttggcg aacaatacga acatctgaag atgtgttaac cactggtgca gttgtttcag gcattgaaat acgtgcacga ggaacatcta ctgatccttt caagcttaaa cgcttatcaa aaggatacat atcaagtgtc aaattcggat ttgcaatggc cgtaattaat ggagcttgac gtacaagtaa gttatcacct tttaagttta actgtagacg cggtgcattt ttccagtcaa aactaccttt aagtaaccct acaccttgtc cactattgaa tgcaccatcc atatggtggt ggtggtggtg agccat
Expected Protein Sequence
MAHHHHHHDG AFNSGQGVGL LKGSFDWKNA PRLQLNLKGD NLLVRQAPLI TAIANPNLTL DMYPFDKRLS LKGSVDVPRA RISMPETTAP VVNTSSDVRI VRQGQDPLAI LRAAKPWDIR ADISVNIGNQ VIFQGFNSNI PLVGRLNLTQ RGYETAMRAM GAIGVSQKVK IEAYGQSLDL NRAIARFNGP LANPTLDIDA NKNVQGSMVG VRVTGTASSP NIQVYNDAGL SEQEALNALV TGRINEGASG LSNAEGFKSD VNNTIAAAGI SMGLGGTRAL TNQIGRTFGL SGLALDAQGT GDDTQVSLTG YITPDLFIRY GVGVFTPVNK LTLRYQMNRR LYLEASQSLE RAIDLFYNWR F
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgatggtg cattcaatag tggacaaggt gtagggttac ttaaaggtag ttttgactgg aaaaatgcac cgcgtctaca gttaaactta aaaggtgata acttacttgt acgtcaagct ccattaatta cggccattgc aaatccgaat ttgacacttg atatgtatcc ttttgataag cgtttaagct tgaaaggatc agtagatgtt cctcgtgcac gtatttcaat gcctgaaaca actgcaccag tggttaacac atcttcagat gttcgtattg ttcgccaagg tcaggaccca cttgcaattt tacgtgcggc aaaaccgtgg gatatccgtg cagatatttc ggtaaatatt ggcaatcaag tcatcttcca aggctttaac agtaatattc ctttagtggg gcgtttgaat ttaacccaac gcggttatga aacagcaatg cgagcaatgg gtgctattgg tgtaagtcaa aaagtgaaaa ttgaggccta tggtcaaagc ctagatttaa accgtgcaat tgcccgtttt aatggcccat tggcaaaccc gacgttagat attgatgcga ataaaaatgt tcagggtagt atggttgggg ttcgtgtcac tggtactgct tcatcaccaa acattcaagt ttataatgat gcaggccttt ctgaacaaga ggcattaaat gcactcgtga cagggcgtat taatgaaggt gccagtggtt taagtaatgc agaaggcttt aaatctgatg tgaacaatac aattgctgct gccggtatta gtatgggctt gggtggtact cgtgctttaa cgaatcaaat tggtcgtact tttggtttga gtggtcttgc attagatgct caaggtacag gagatgatac tcaagtaagt ttaactggtt acattacacc tgatttattt attcgttatg gtgtgggtgt gtttactcca gtcaataaac tgacgttgcg ttatcaaatg aaccgccgtt tgtatttgga agcaagtcaa tctttagaac gagcaataga tttattctac aattggcgat tctgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for AcbaC.19028.a.B3.GE39047
HARVESTED ON: 3/31/2015
SEQUENCED ON: 3/27/2015
EXPECTED MW: 54kDa
OBSERVED MW: 54kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMD MGYLSVHNAT GNMDFSGKYP LNATADLRIP SLKSLNIQNI KVAARGSLDT LQAGVATTTP DLLTGWVVLH PVRHEVPMQG ALLLKNYHLP LLVEQKLFAK NGVIKFQGDI KQLNLALETD LKGENLPEGQ YNALMNTDLV HQLNITDFNG QVMKGAVNLK GLVNWKDHVT WDIKGRLDHV NPKDKAIPQV VQDFLPPSLD AAVSSTGSLE KGTEVFANVD FDRYESWKLK LNQAPEKNKK PQPMFMNVAW ANIDRAMPYI GWLSSDSGQV DLTLRDGQQD IKVSTKVYQH EQTLLPAGQY LATLNVKDNI LNVPSFSFAA QKGSLTGQAK VLLPSEKRQL AWNALLNAKD FNPQSIHTAA PVNLLNGSIK ASGFAKPNQQ IIQLEKIDLK GRLAQAGQEM VALAGKSTAA LLFNDVKAGG GFKGFAVNYD GSLKALNQAN GLLKFSIAGT PEFIRISQLQ HDGVAGKIYA TGALNLKD
Validated NT Sequence
acggtctttt aaatttaatg ccccagtcgc atagattttc ccagcaacac catcatgttg tagctgacta attcggataa attctggggt acctgcaata gaaaacttta atagtccatt ggcttgatta agtgctttta aagatccgtc ataattgaca gcaaagcctt taaaaccgcc acctgcttta acatcattaa ataataaagc tgctgtactt tttccagcta gcgcaaccat ttcttgacct gcttgagcca agcgaccttt taaatcaatt ttttcaagct gaataatttg ttgatttggc tttgcaaaac cgctggcttt aattgatccg ttgagcaagt taacaggcgc cgctgtatgt atactttgtg gattaaagtc ttttgcattt aataatgcat tccatgccag ctgacgtttt tcacttggta ataagacttt ggcctgacct gtgaggctac ccttttgagc cgcaaaacta aaactaggaa cattaagaat attatcttta acattcaaag ttgctaaata ttgtccagcc ggtaataagg tctgttcatg ctgatatacc tttgtcgaaa ctttaatatc ttgctgaccg tctcgcaaag tcaaatcaac ttgaccacta tcactactta accaacctat atatggcata gcacgatcta tatttgccca agccacattc ataaacatag gttgtggctt cttattcttt tcaggtgctt ggtttagttt aagtttccaa gattcatagc ggtcaaagtc tacattggca aatacttccg tacctttttc caaagagcca gttgaagaaa cggctgcatc tagacttggc ggcaagaaat cctgaacgac ctgtggaata gctttgtctt taggattaac atgatctagg cgacctttga tgtcccaagt gacatgatct ttccagttca ctaatccttt aagattaact gcacccttca tgacctgccc gttaaaatca gtaatattca actgatgaac cagatcagta ttcattaagg cgttgtactg gccttcaggt aaattctccc cttttaaatc tgtctcaagt gctaagttga gttgcttaat atcaccctga aatttaatta caccgttttt agcaaataat ttttgctcaa ccagtaaagg caagtgataa tttttgagta acaatgcacc ttgcataggc acttcatgac gaacaggatg taaaacaacc caacctgtta ataaatctgg tgttgtcgtt gctacaccgg cttgcaaagt atctaaacta ccccgcgctg ccaccttaat attttggatg tttaaacttt ttaaagaagg aattcttaaa tctgcagtcg cattcagtgg gtatttgccg ctaaagtcca tatttcctgt tgcgttatga acagaaagat accccatatc catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHMD MGYLSVHNAT GNMDFSGKYP LNATADLRIP SLKSLNIQNI KVAARGSLDT LQAGVATTTP DLLTGWVVLH PVRHEVPMQG ALLLKNYHLP LLVEQKLFAK NGVIKFQGDI KQLNLALETD LKGENLPEGQ YNALMNTDLV HQLNITDFNG QVMKGAVNLK GLVNWKDHVT WDIKGRLDHV NPKDKAIPQV VQDFLPPSLD AAVSSTGSLE KGTEVFANVD FDRYESWKLK LNQAPEKNKK PQPMFMNVAW ANIDRAMPYI GWLSSDSGQV DLTLRDGQQD IKVSTKVYQH EQTLLPAGQY LATLNVKDNI LNVPSFSFAA QKGSLTGQAK VLLPSEKRQL AWNALLNAKD FNPQSIHTAA PVNLLNGSIK ASGFAKPNQQ IIQLEKIDLK GRLAQAGQEM VALAGKSTAA LLFNDVKAGG GFKGFAVNYD GSLKALNQAN GLLKFSIAGT PEFIRISQLQ HDGVAGKIYA TGALNLKDRI AWDINS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggata tggggtatct ttctgttcat aacgcaacag gaaatatgga ctttagcggc aaatacccac tgaatgcgac tgcagattta agaattcctt ctttaaaaag tttaaacatc caaaatatta aggtggcagc gcggggtagt ttagatactt tgcaagccgg tgtagcaacg acaacaccag atttattaac aggttgggtt gttttacatc ctgttcgtca tgaagtgcct atgcaaggtg cattgttact caaaaattat cacttgcctt tactggttga gcaaaaatta tttgctaaaa acggtgtaat taaatttcag ggtgatatta agcaactcaa cttagcactt gagacagatt taaaagggga gaatttacct gaaggccagt acaacgcctt aatgaatact gatctggttc atcagttgaa tattactgat tttaacgggc aggtcatgaa gggtgcagtt aatcttaaag gattagtgaa ctggaaagat catgtcactt gggacatcaa aggtcgccta gatcatgtta atcctaaaga caaagctatt ccacaggtcg ttcaggattt cttgccgcca agtctagatg cagccgtttc ttcaactggc tctttggaaa aaggtacgga agtatttgcc aatgtagact ttgaccgcta tgaatcttgg aaacttaaac taaaccaagc acctgaaaag aataagaagc cacaacctat gtttatgaat gtggcttggg caaatataga tcgtgctatg ccatatatag gttggttaag tagtgatagt ggtcaagttg atttgacttt gcgagacggt cagcaagata ttaaagtttc gacaaaggta tatcagcatg aacagacctt attaccggct ggacaatatt tagcaacttt gaatgttaaa gataatattc ttaatgttcc tagttttagt tttgcggctc aaaagggtag cctcacaggt caggccaaag tcttattacc aagtgaaaaa cgtcagctgg catggaatgc attattaaat gcaaaagact ttaatccaca aagtatacat acagcggcgc ctgttaactt gctcaacgga tcaattaaag ccagcggttt tgcaaagcca aatcaacaaa ttattcagct tgaaaaaatt gatttaaaag gtcgcttggc tcaagcaggt caagaaatgg ttgcgctagc tggaaaaagt acagcagctt tattatttaa tgatgttaaa gcaggtggcg gttttaaagg ctttgctgtc aattatgacg gatctttaaa agcacttaat caagccaatg gactattaaa gttttctatt gcaggtaccc cagaatttat ccgaattagt cagctacaac atgatggtgt tgctgggaaa atctatgcga ctggggcatt aaatttaaaa gaccgtattg cttgggatat taatagctga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for AcbaC.19028.a.B4.GE39048
HARVESTED ON: 3/31/2015
SEQUENCED ON: 3/27/2015
EXPECTED MW: 53kDa
OBSERVED MW: 53kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMS LVRFKPQYFA SSVKGEISGN VKTQGIWSDK LKRIDIQQLN LAGYLNNKPV RGKGNLSILM DSNQKGFLPQ QFEANNLFLV YAQNQLQATG NAQNLKIKLN APALYDLYPG LSGRAYGDLS VQSQPRLKAT ANIAVDNFAF NTLVSIKKLR IQGELPTSET TPTQLTAKLD NLRSGSRQIQ SAEVNLTGTR KAHLLKVQGN NNVSKFYVQL AGGFNAKNDW LGQIQKGSFD SRRIRLAQNQ NAPVVFSSAR SELYVGQHCW QSTNSQLCLD QPVRVSKAQG NISFVTQNLD LGDFAAFMPE GLAMTGQLNG YAKASWVNGG HPKLDARLVT RKGELGLAAE DPQDPPTTLA YDELSVIAKS ISEGLLFRVD VKTPDIGTGY ANVIINPYQS SMPMHGEVAF NDVQLKVLKP FIQDVRSMSG TLALAGKVNG TLTQPQFTGE MRLKNGAISM ISLPVNLTNV QVYSS
Validated NT Sequence
atagatgaat acacctgtac attggttaaa ttaaccggca gggaaatcat actgatcgcc ccgtttttca aacgcatttc gcccgtaaac tgtggctggg tcaaagtacc attcactttg ccagctaagg ctaaggtacc actcattgaa cgtacatctt gaatgaatgg ttttaacact tttaactgta catcgttaaa agctacctca ccatgcattg gcatagatga ttggtatgga ttaataataa cattcgcata tcctgtacca atatcaggtg tttttacatc gacacggaac aataagcctt ctgaaatact ttttgcaatt acactcagtt catcataagc caaagtagtc ggtggatctt gcgggtcttc cgctgctaga cccagttcac cttttctggt tactaagcgt gcatcaagct taggatgccc accatttacc caagaagctt tagcataacc attaagttgg cctgtcatag ccaaaccttc cggcatgaac gcagcaaaat cacctaaatc tagattttga gtaacaaatg aaatattacc ttgtgcttta ctcacacgca caggctggtc aagacacaac tggctatttg tactttgcca gcaatgttgc ccaacatata attcagaacg tgccgatgag aaaacaactg gtgcattttg attttgtgct agacgaattc ggcgtgaatc aaaacttcct ttttgaattt gccctaacca atcattcttc gcattaaatc cacctgctaa ctgaacatag aatttagaaa cattattatt tccttgcact tttaataaat gtgcttttct tgttcctgtt aaattcacct cagcagactg aatttgacgg ctaccactac gtaaattgtc taattttgct gttaactgag ttggtgttgt ttcggaagta ggcaattcac cctggatacg aagtttttta atactgacta atgtattaaa tgcaaaatta tcaaccgcaa tattcgccgt agctttcaaa cgcggttgag attggacact caagtctcca tatgcacgcc cacttaaacc cggatacaaa tcataaagcg caggtgcatt cagcttaatt tttagattct gtgcattacc agtcgcctgc aactggtttt gcgcataaac taaaaataga ttatttgctt caaattgctg aggtaaaaag cctttttggt tcgaatccat aagtatggat aagttacctt tacctctgac tggcttatta ttcaaataac ccgcaaggtt caattgttga atatcaatac gctttaactt gtcagaccag atgccttgtg tttttacatt tcctgaaatt tcgcctttta cactagaggc aaaatattga ggtttaaagc gtactaacga catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHSL VRFKPQYFAS SVKGEISGNV KTQGIWSDKL KRIDIQQLNL AGYLNNKPVR GKGNLSILMD SNQKGFLPQQ FEANNLFLVY AQNQLQATGN AQNLKIKLNA PALYDLYPGL SGRAYGDLSV QSQPRLKATA NIAVDNFAFN TLVSIKKLRI QGELPTSETT PTQLTAKLDN LRSGSRQIQS AEVNLTGTRK AHLLKVQGNN NVSKFYVQLA GGFNAKNDWL GQIQKGSFDS RRIRLAQNQN APVVFSSARS ELYVGQHCWQ STNSQLCLDQ PVRVSKAQGN ISFVTQNLDL GDFAAFMPEG LAMTGQLNGY AKASWVNGGH PKLDARLVTR KGELGLAAED PQDPPTTLAY DELSVIAKSI SEGLLFRVDV KTPDIGTGYA NVIINPYQSS MPMHGEVAFN DVQLKVLKPF IQDVRSMSGT LALAGKVNGT LTQPQFTGEM RLKNGAISMI SLPVNLTNVQ VYSSIHQDMA TI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattcgttag tacgctttaa acctcaatat tttgcctcta gtgtaaaagg cgaaatttca ggaaatgtaa aaacacaagg catctggtct gacaagttaa agcgtattga tattcaacaa ttgaaccttg cgggttattt gaataataag ccagtcagag gtaaaggtaa cttatccata cttatggatt cgaaccaaaa aggcttttta cctcagcaat ttgaagcaaa taatctattt ttagtttatg cgcaaaacca gttgcaggcg actggtaatg cacagaatct aaaaattaag ctgaatgcac ctgcgcttta tgatttgtat ccgggtttaa gtgggcgtgc atatggagac ttgagtgtcc aatctcaacc gcgtttgaaa gctacggcga atattgcggt tgataatttt gcatttaata cattagtcag tattaaaaaa cttcgtatcc agggtgaatt gcctacttcc gaaacaacac caactcagtt aacagcaaaa ttagacaatt tacgtagtgg tagccgtcaa attcagtctg ctgaggtgaa tttaacagga acaagaaaag cacatttatt aaaagtgcaa ggaaataata atgtttctaa attctatgtt cagttagcag gtggatttaa tgcgaagaat gattggttag ggcaaattca aaaaggaagt tttgattcac gccgaattcg tctagcacaa aatcaaaatg caccagttgt tttctcatcg gcacgttctg aattatatgt tgggcaacat tgctggcaaa gtacaaatag ccagttgtgt cttgaccagc ctgtgcgtgt gagtaaagca caaggtaata tttcatttgt tactcaaaat ctagatttag gtgattttgc tgcgttcatg ccggaaggtt tggctatgac aggccaactt aatggttatg ctaaagcttc ttgggtaaat ggtgggcatc ctaagcttga tgcacgctta gtaaccagaa aaggtgaact gggtctagca gcggaagacc cgcaagatcc accgactact ttggcttatg atgaactgag tgtaattgca aaaagtattt cagaaggctt attgttccgt gtcgatgtaa aaacacctga tattggtaca ggatatgcga atgttattat taatccatac caatcatcta tgccaatgca tggtgaggta gcttttaacg atgtacagtt aaaagtgtta aaaccattca ttcaagatgt acgttcaatg agtggtacct tagccttagc tggcaaagtg aatggtactt tgacccagcc acagtttacg ggcgaaatgc gtttgaaaaa cggggcgatc agtatgattt ccctgccggt taatttaacc aatgtacagg tgtattcatc tattcatcag gacatggcaa caatttgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat