AcbaC.19027.a

transcriptional regulator, AraC family

CENTER ID: AcbaC.19027.a
ORGANISM: Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AcbaC.19027.a.B1.GE39045 full length 1 336
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AcbaC.19027.a.B1.PW38002 full length 1 336

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|470.1345.peg.609
GenBank: AKA30409.1
PGAT: acinetobacter:ABUW_0639
RefSeq: WP_000085721.1
UniProt: A0A059ZLB2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSRDTISIHF VNAALTGVKR LGMDVETLLS HVGIEAELLR QPKARISPEQ YTRFIKMLWM VTQDEHVGFD VQPRRLGTFA IMCQLIIHAK TLGQALELSS QFYKLFGDEW SVTLERDKHE ARLVPLIPKT LDPDHFITES MLMIWHGLAS WLIERRLPLE RVHFSYPRPA HADEYDALFF APVMQFDAPR TEITFAADYL DLPIRQDEKT LEEFLKAAPA QLLVKFKNTN SLTSRIREVL KSQIGEEMPT LNDVASMLYL SPQTLRRRLA AEGKSYQGVK DALRRDAAIH LLLNPELTLE DVAQQVGFSE TSTFHRAFKK WTGVTPGLYR QLHGYH
NT Sequence
ATGAGTCGCG ATACGATTAG CATCCACTTC GTGAATGCGG CTTTGACAGG CGTAAAGCGC CTTGGCATGG ATGTCGAGAC GTTACTTTCA CATGTGGGTA TCGAAGCGGA ATTATTACGT CAACCTAAAG CTCGAATCTC ACCAGAACAA TACACTCGTT TTATAAAAAT GTTGTGGATG GTCACACAGG ATGAACACGT TGGATTTGAC GTACAACCGC GTCGTCTGGG CACTTTTGCC ATTATGTGTC AGTTGATTAT TCACGCAAAA ACATTGGGCC AAGCCCTTGA ACTGTCATCT CAATTCTACA AATTATTTGG CGATGAATGG TCTGTAACTC TAGAGCGTGA CAAACATGAA GCACGTCTAG TTCCCCTCAT TCCAAAAACT TTAGATCCAG ACCATTTCAT TACTGAAAGC ATGCTCATGA TTTGGCACGG TCTAGCATCA TGGTTGATTG AACGTCGTTT GCCATTAGAA CGTGTTCATT TTAGCTATCC ACGTCCTGCA CATGCCGATG AATATGATGC ATTATTTTTT GCACCAGTTA TGCAGTTTGA TGCACCACGT ACTGAAATTA CCTTTGCAGC TGATTATCTT GATTTGCCAA TCCGCCAAGA TGAGAAAACG CTAGAAGAAT TCTTAAAAGC AGCTCCTGCT CAGCTTTTAG TAAAATTCAA AAACACCAAC TCGCTAACTT CTCGTATTCG TGAAGTGCTC AAAAGCCAAA TTGGTGAAGA AATGCCAACG CTTAATGACG TGGCATCAAT GTTGTATTTA TCTCCGCAAA CCTTACGCCG CCGCTTGGCT GCCGAAGGTA AAAGCTACCA AGGCGTAAAA GATGCCCTAC GCCGAGATGC TGCGATTCAC TTGTTACTCA ACCCTGAGTT AACACTTGAA GATGTTGCCC AACAAGTTGG CTTTAGTGAA ACCAGTACTT TCCACCGTGC ATTTAAAAAG TGGACGGGTG TAACCCCTGG CCTATATCGC CAGCTACATG GTTATCACTA A
Details for AcbaC.19027.a.B1.GE39045
HARVESTED ON: 3/31/2015
SEQUENCED ON: 3/27/2015
EXPECTED MW: 40kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMS RDTISIHFVN AALTGVKRLG MDVETLLSHV GIEAELLRQP KARISPEQYT RFIKMLWMVT QDEHVGFDVQ PRRLGTFAIM CQLIIHAKTL GQALELSSQF YKLFGDEWSV TLERDKHEAR LVPLIPKTLD PDHFITESML MIWHGLASWL IERRLPLERV HFSYPRPAHA DEYDALFFAP VMQFDAPRTE ITFAADYLDL PIRQDEKTLE EFLKAAPAQL LVKFKNTNSL TSRIREVLKS QIGEEMPTLN DVASMLYLSP QTLRRRLAAE GKSYQGVKDA LRRDAAIHLL LNPELTLEDV AQQVGFSETS TFHRAFKKWT GVTPGL
Validated NT Sequence
atataggcca ggggttacac ccgtccactt tttaaatgca cggtggaaag tactggtttc actaaagcca acttgttggg caacatcttc aagtgttaac tcagggttga gtaacaagtg aatcgcagca tctcggcgta gggcatcttt tacgccttgg tagcttttac cttcggcagc caagcggcgg cgtaaggttt gcggagataa atacaacatt gatgccacgt cattaagcgt tggcatttct tcaccaattt ggcttttgag cacttcacga atacgagaag ttagcgagtt ggtgtttttg aattttacta aaagctgagc aggagctgct tttaagaatt cttctagcgt tttctcatct tggcggattg gcaaatcaag ataatcagct gcaaaggtaa tttcagtacg tggtgcatca aactgcataa ctggtgcaaa aaataatgca tcatattcat cggcatgtgc aggacgtgga tagctaaaat gaacacgttc taatggcaaa cgacgttcaa tcaaccatga tgctagaccg tgccaaatca tgagcatgct ttcagtaatg aaatggtctg gatctaaagt ttttggaatg aggggaacta gacgtgcttc atgtttgtca cgctctagag ttacagacca ttcatcgcca aataatttgt agaattgaga tgacagttca agggcttggc ccaatgtttt tgcgtgaata atcaactgac acataatggc aaaagtgccc agacgacgcg gttgtacgtc aaatccaacg tgttcatcct gtgtgaccat ccacaacatt tttataaaac gagtgtattg ttctggtgag attcgagctt taggttgacg taataattcc gcttcgatac ccacatgtga aagtaacgtc tcgacatcca tgccaaggcg ctttacgcct gtcaaagccg cattcacgaa gtggatgcta atcgtatcgc gactcatatg gtggtggtgg tggtgagcca t
Expected Protein Sequence
MAHHHHHHMS RDTISIHFVN AALTGVKRLG MDVETLLSHV GIEAELLRQP KARISPEQYT RFIKMLWMVT QDEHVGFDVQ PRRLGTFAIM CQLIIHAKTL GQALELSSQF YKLFGDEWSV TLERDKHEAR LVPLIPKTLD PDHFITESML MIWHGLASWL IERRLPLERV HFSYPRPAHA DEYDALFFAP VMQFDAPRTE ITFAADYLDL PIRQDEKTLE EFLKAAPAQL LVKFKNTNSL TSRIREVLKS QIGEEMPTLN DVASMLYLSP QTLRRRLAAE GKSYQGVKDA LRRDAAIHLL LNPELTLEDV AQQVGFSETS TFHRAFKKWT GVTPGLYRQL HGYH
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagtc gcgatacgat tagcatccac ttcgtgaatg cggctttgac aggcgtaaag cgccttggca tggatgtcga gacgttactt tcacatgtgg gtatcgaagc ggaattatta cgtcaaccta aagctcgaat ctcaccagaa caatacactc gttttataaa aatgttgtgg atggtcacac aggatgaaca cgttggattt gacgtacaac cgcgtcgtct gggcactttt gccattatgt gtcagttgat tattcacgca aaaacattgg gccaagccct tgaactgtca tctcaattct acaaattatt tggcgatgaa tggtctgtaa ctctagagcg tgacaaacat gaagcacgtc tagttcccct cattccaaaa actttagatc cagaccattt cattactgaa agcatgctca tgatttggca cggtctagca tcatggttga ttgaacgtcg tttgccatta gaacgtgttc attttagcta tccacgtcct gcacatgccg atgaatatga tgcattattt tttgcaccag ttatgcagtt tgatgcacca cgtactgaaa ttacctttgc agctgattat cttgatttgc caatccgcca agatgagaaa acgctagaag aattcttaaa agcagctcct gctcagcttt tagtaaaatt caaaaacacc aactcgctaa cttctcgtat tcgtgaagtg ctcaaaagcc aaattggtga agaaatgcca acgcttaatg acgtggcatc aatgttgtat ttatctccgc aaaccttacg ccgccgcttg gctgccgaag gtaaaagcta ccaaggcgta aaagatgccc tacgccgaga tgctgcgatt cacttgttac tcaaccctga gttaacactt gaagatgttg cccaacaagt tggctttagt gaaaccagta ctttccaccg tgcatttaaa aagtggacgg gtgtaacccc tggcctatat cgccagctac atggttatca ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for AcbaC.19027.a.B1.PW38002
PURIFICATION DATe: 12/21/2016
CONCENTRATION: 17mg/ml
OBSERVED MW: 35kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 8.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 11
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS RDTISIHFVN AALTGVKRLG MDVETLLSHV GIEAELLRQP KARISPEQYT RFIKMLWMVT QDEHVGFDVQ PRRLGTFAIM CQLIIHAKTL GQALELSSQF YKLFGDEWSV TLERDKHEAR LVPLIPKTLD PDHFITESML MIWHGLASWL IERRLPLERV HFSYPRPAHA DEYDALFFAP VMQFDAPRTE ITFAADYLDL PIRQDEKTLE EFLKAAPAQL LVKFKNTNSL TSRIREVLKS QIGEEMPTLN DVASMLYLSP QTLRRRLAAE GKSYQGVKDA LRRDAAIHLL LNPELTLEDV AQQVGFSETS TFHRAFKKWT GVTPGL
Validated NT Sequence
atataggcca ggggttacac ccgtccactt tttaaatgca cggtggaaag tactggtttc actaaagcca acttgttggg caacatcttc aagtgttaac tcagggttga gtaacaagtg aatcgcagca tctcggcgta gggcatcttt tacgccttgg tagcttttac cttcggcagc caagcggcgg cgtaaggttt gcggagataa atacaacatt gatgccacgt cattaagcgt tggcatttct tcaccaattt ggcttttgag cacttcacga atacgagaag ttagcgagtt ggtgtttttg aattttacta aaagctgagc aggagctgct tttaagaatt cttctagcgt tttctcatct tggcggattg gcaaatcaag ataatcagct gcaaaggtaa tttcagtacg tggtgcatca aactgcataa ctggtgcaaa aaataatgca tcatattcat cggcatgtgc aggacgtgga tagctaaaat gaacacgttc taatggcaaa cgacgttcaa tcaaccatga tgctagaccg tgccaaatca tgagcatgct ttcagtaatg aaatggtctg gatctaaagt ttttggaatg aggggaacta gacgtgcttc atgtttgtca cgctctagag ttacagacca ttcatcgcca aataatttgt agaattgaga tgacagttca agggcttggc ccaatgtttt tgcgtgaata atcaactgac acataatggc aaaagtgccc agacgacgcg gttgtacgtc aaatccaacg tgttcatcct gtgtgaccat ccacaacatt tttataaaac gagtgtattg ttctggtgag attcgagctt taggttgacg taataattcc gcttcgatac ccacatgtga aagtaacgtc tcgacatcca tgccaaggcg ctttacgcct gtcaaagccg cattcacgaa gtggatgcta atcgtatcgc gactcatatg gtggtggtgg tggtgagcca t
Expressed Protein Sequence
MAHHHHHHMS RDTISIHFVN AALTGVKRLG MDVETLLSHV GIEAELLRQP KARISPEQYT RFIKMLWMVT QDEHVGFDVQ PRRLGTFAIM CQLIIHAKTL GQALELSSQF YKLFGDEWSV TLERDKHEAR LVPLIPKTLD PDHFITESML MIWHGLASWL IERRLPLERV HFSYPRPAHA DEYDALFFAP VMQFDAPRTE ITFAADYLDL PIRQDEKTLE EFLKAAPAQL LVKFKNTNSL TSRIREVLKS QIGEEMPTLN DVASMLYLSP QTLRRRLAAE GKSYQGVKDA LRRDAAIHLL LNPELTLEDV AQQVGFSETS TFHRAFKKWT GVTPGLYRQL HGYH
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGTC GCGATACGAT TAGCATCCAC TTCGTGAATG CGGCTTTGAC AGGCGTAAAG CGCCTTGGCA TGGATGTCGA GACGTTACTT TCACATGTGG GTATCGAAGC GGAATTATTA CGTCAACCTA AAGCTCGAAT CTCACCAGAA CAATACACTC GTTTTATAAA AATGTTGTGG ATGGTCACAC AGGATGAACA CGTTGGATTT GACGTACAAC CGCGTCGTCT GGGCACTTTT GCCATTATGT GTCAGTTGAT TATTCACGCA AAAACATTGG GCCAAGCCCT TGAACTGTCA TCTCAATTCT ACAAATTATT TGGCGATGAA TGGTCTGTAA CTCTAGAGCG TGACAAACAT GAAGCACGTC TAGTTCCCCT CATTCCAAAA ACTTTAGATC CAGACCATTT CATTACTGAA AGCATGCTCA TGATTTGGCA CGGTCTAGCA TCATGGTTGA TTGAACGTCG TTTGCCATTA GAACGTGTTC ATTTTAGCTA TCCACGTCCT GCACATGCCG ATGAATATGA TGCATTATTT TTTGCACCAG TTATGCAGTT TGATGCACCA CGTACTGAAA TTACCTTTGC AGCTGATTAT CTTGATTTGC CAATCCGCCA AGATGAGAAA ACGCTAGAAG AATTCTTAAA AGCAGCTCCT GCTCAGCTTT TAGTAAAATT CAAAAACACC AACTCGCTAA CTTCTCGTAT TCGTGAAGTG CTCAAAAGCC AAATTGGTGA AGAAATGCCA ACGCTTAATG ACGTGGCATC AATGTTGTAT TTATCTCCGC AAACCTTACG CCGCCGCTTG GCTGCCGAAG GTAAAAGCTA CCAAGGCGTA AAAGATGCCC TACGCCGAGA TGCTGCGATT CACTTGTTAC TCAACCCTGA GTTAACACTT GAAGATGTTG CCCAACAAGT TGGCTTTAGT GAAACCAGTA CTTTCCACCG TGCATTTAAA AAGTGGACGG GTGTAACCCC TGGCCTATAT CGCCAGCTAC ATGGTTATCA CTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT