SSGCID: Target Page

AcbaC.19026.a

transcriptional regulator, HTH_3 family

CENTER ID:	AcbaC.19026.a
ORGANISM:	Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AcbaC.19026.a.B1.GE39044	full length		1	381

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|470.1345.peg.3253
GenBank:	AKA33015.1
PGAT:	acinetobacter:ABUW_3319
RefSeq:	WP_000482796.1
UniProt:	A0A090BE98

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MFNMGRFDLA RQRRGLTKRD LAHLLGVTDR TVSNWYSNQE IDERILEKAA EILDFPLSFF YGNDIEKIHA ESVSFRALTK MTARKRDMAI SQTILAEMIS DWIDLKFELP LPNVPDLHEL RSDFSSSSID ELDEVSEDEV NYYLKYSYPE ACADTVRKAW GLGEQPIPNL IALLESKGIR VFSLTDEAQD VDACCRWTSG RPFIFLNTSR TAERCRFDLA HELGHLVMHK HGIIEGIHVE QEANAFASAF LMPRRSLLAD PLKIPSLKGI LSKKEIWQVS AAALTYRYNK LGIITDWNAT SIYKQLAQRG RNNEPNPIAH ETSLLLSKIF QALAQENFDL SEMTNELGLN LDEINRLTFN LVSKYQNLEA LRRRSQLIVL N

NT Sequence

ATGTTCAATA TGGGTCGTTT CGATCTCGCT CGTCAACGCC GTGGCTTGAC TAAGAGAGAT TTGGCTCATC TATTAGGCGT AACCGATAGA ACTGTTTCAA ATTGGTATAG TAACCAAGAA ATTGATGAGA GAATTTTAGA AAAGGCTGCT GAGATTCTTG ATTTTCCTCT CAGCTTTTTT TATGGAAATG ATATTGAGAA AATTCATGCT GAGTCTGTAA GTTTTAGAGC TCTTACTAAA ATGACAGCTA GAAAAAGAGA TATGGCAATT AGCCAAACTA TTTTAGCTGA AATGATCAGT GATTGGATTG ATTTGAAATT TGAATTACCT CTTCCTAACG TTCCTGATTT ACATGAACTA AGAAGTGATT TTTCGTCCTC TTCTATTGAT GAATTAGATG AAGTCAGTGA AGATGAGGTT AATTATTATT TAAAATATTC TTATCCTGAA GCATGCGCAG ATACAGTAAG AAAAGCGTGG GGATTAGGAG AACAGCCCAT CCCAAACTTA ATTGCACTTT TAGAATCTAA GGGCATTAGA GTTTTTTCTC TTACAGATGA AGCACAAGAT GTTGATGCAT GTTGTAGATG GACAAGTGGT CGGCCTTTTA TTTTTTTAAA TACCTCTCGA ACTGCTGAAA GATGTCGATT CGACTTAGCT CATGAGCTTG GTCATTTAGT GATGCATAAG CACGGAATAA TTGAAGGTAT TCATGTTGAA CAAGAAGCTA ACGCTTTTGC TTCCGCATTT TTAATGCCAC GTAGAAGCTT ACTAGCAGAT CCTTTAAAAA TACCTTCATT AAAGGGTATT TTATCCAAGA AAGAAATATG GCAAGTTTCT GCTGCTGCTT TGACTTATCG TTACAATAAG CTCGGTATTA TTACTGATTG GAACGCGACT AGTATATATA AACAATTGGC TCAGAGAGGA CGAAATAACG AGCCAAACCC TATTGCTCAT GAAACGTCTC TTTTACTTAG TAAAATTTTT CAAGCTTTAG CTCAGGAAAA TTTTGATTTA TCAGAAATGA CTAATGAGTT AGGTCTTAAT CTAGATGAAA TTAATAGGCT TACTTTTAAT CTTGTATCTA AATATCAAAA TTTAGAAGCA CTACGTCGTC GAAGTCAGTT AATAGTTTTG AACTGA

Details for AcbaC.19026.a.B1.GE39044

HARVESTED ON:	3/31/2015
SEQUENCED ON:	3/27/2015
EXPECTED MW:	45kDa
OBSERVED MW:	45kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMF NMGRFDLARQ RRGLTKRDLA HLLGVTDRTV SNWYSNQEID ERILEKAAEI LDFPLSFFYG NDIEKIHAES VSFRALTKMT ARKRDMAISQ TILAEMISDW IDLKFELPLP NVPDLHELRS DFSSSSIDEL DEVSEDEVNY YLKYSYPEAC ADTVRKAWGL GEQPIPNLIA LLESKGIRVF SLTDEAQDVD ACCRWTSGRP FIFLNTSRTA ERCRFDLAHE LGHLVMHKHG IIEGIHVEQE ANAFASAFLM PRRSLLADPL KIPSLKGILS KKEIWQVSAA ALTYRYNKLG IITDWNATSI YKQLAQRGRN NEPNPIAHET SLLLSKIFQA LAQENFDLSE MTNELGLNLD EINRLTFNLV SKYQNLEALR RX

Validated NT Sequence

ttnnacgacg tagtgcttct aaattttgat atttagatac aagattaaaa gtaagcctat taatttcatc tagattaaga cctaactcat tagtcatttc tgataaatca aaattttcct gagctaaagc ttgaaaaatt ttactaagta aaagagacgt ttcatgagca atagggtttg gctcgttatt tcgtcctctc tgagccaatt gtttatatat actagtcgcg ttccaatcag taataatacc gagcttattg taacgataag tcaaagcagc agcagaaact tgccatattt ctttcttgga taaaataccc tttaatgaag gtatttttaa aggatctgct agtaagcttc tacgtggcat taaaaatgcg gaagcaaaag cgttagcttc ttgttcaaca tgaatacctt caattattcc gtgcttatgc atcactaaat gaccaagctc atgagctaag tcgaatcgac atctttcagc agttcgagag gtatttaaaa aaataaaagg ccgaccactt gtccatctac aacatgcatc aacatcttgt gcttcatctg taagagaaaa aactctaatg cccttagatt ctaaaagtgc aattaagttt gggatgggct gttctcctaa tccccacgct tttcttactg tatctgcgca tgcttcagga taagaatatt ttaaataata attaacctca tcttcactga cttcatctaa ttcatcaata gaagaggacg aaaaatcact tcttagttca tgtaaatcag gaacgttagg aagaggtaat tcaaatttca aatcaatcca atcactgatc atttcagcta aaatagtttg gctaattgcc atatctcttt ttctagctgt cattttagta agagctctaa aacttacaga ctcagcatga attttctcaa tatcatttcc ataaaaaaag ctgagaggaa aatcaagaat ctcagcagcc ttttctaaaa ttctctcatc aatttcttgg ttactatacc aatttgaaac agttctatcg gttacgccta atagatgagc caaatctctc ttagtcaagc cacggcgttg acgagcgaga tcgaaacgac ccatattgaa catatggtgg tggtggtggt gagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttca atatgggtcg tttcgatctc gctcgtcaac gccgtggctt gactaagaga gatttggctc atctattagg cgtaaccgat agaactgttt caaattggta tagtaaccaa gaaattgatg agagaatttt agaaaaggct gctgagattc ttgattttcc tctcagcttt ttttatggaa atgatattga gaaaattcat gctgagtctg taagttttag agctcttact aaaatgacag ctagaaaaag agatatggca attagccaaa ctattttagc tgaaatgatc agtgattgga ttgatttgaa atttgaatta cctcttccta acgttcctga tttacatgaa ctaagaagtg atttttcgtc ctcttctatt gatgaattag atgaagtcag tgaagatgag gttaattatt atttaaaata ttcttatcct gaagcatgcg cagatacagt aagaaaagcg tggggattag gagaacagcc catcccaaac ttaattgcac ttttagaatc taagggcatt agagtttttt ctcttacaga tgaagcacaa gatgttgatg catgttgtag atggacaagt ggtcggcctt ttattttttt aaatacctct cgaactgctg aaagatgtcg attcgactta gctcatgagc ttggtcattt agtgatgcat aagcacggaa taattgaagg tattcatgtt gaacaagaag ctaacgcttt tgcttccgca tttttaatgc cacgtagaag cttactagca gatcctttaa aaataccttc attaaagggt attttatcca agaaagaaat atggcaagtt tctgctgctg ctttgactta tcgttacaat aagctcggta ttattactga ttggaacgcg actagtatat ataaacaatt ggctcagaga ggacgaaata acgagccaaa ccctattgct catgaaacgt ctcttttact tagtaaaatt tttcaagctt tagctcagga aaattttgat ttatcagaaa tgactaatga gttaggtctt aatctagatg aaattaatag gcttactttt aatcttgtat ctaaatatca aaatttagaa gcactacgtc gtcgaagtca gttaatagtt ttgaactgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t