SSGCID: Target Page

AcbaC.19017.a

lipid A biosynthesis acyltransferase

CENTER ID:	AcbaC.19017.a
ORGANISM:	Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS:	cloned
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AcbaC.19017.a.B1.GE39037	full length		1	327

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|470.1345.peg.942
GenBank:	AKA30731.1
PGAT:	acinetobacter:ABUW_0977
UniProt:	A0A0D5YG68

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTRPDSKSMN YQLLKTFSRQ PIQFGRFLAR LLAGLVNTLK ITRTSKSIEL NLRIALPYLT PQQRIAITEK AVRNELTSYF EFLSIWGSSN SKNISRIHRI EGEHFFHEAL AAKKGVVLIV PHFGTWEVMN AWCAQFTSMT ILYKPVKNAD ADRFVREARS REQANLVPTD ESGVRQIFKA LKQGETTVIL PDHTPNVGGD MVNYFGVPLA SSNLSAKLIQ KTKAKALFLY AIRNENDGFT MHIEPMDEKI YEGTADDGTY VIHQAIEQLI YQYPEHYHWS YKRFKANPAL DNIYNIDPTE ALKIVDRLKA EALKTSTQPE PIQTSLM

NT Sequence

ATGACTAGAC CAGATTCAAA AAGCATGAAC TATCAATTAC TCAAAACATT TAGCCGCCAA CCCATTCAAT TTGGGCGTTT TCTTGCTCGT CTGTTAGCAG GACTTGTAAA TACTCTAAAA ATTACTAGAA CATCAAAAAG TATTGAGCTT AACTTAAGAA TTGCGCTTCC GTATTTAACA CCTCAGCAGC GTATAGCGAT TACAGAAAAA GCTGTAAGAA ACGAATTAAC TTCATATTTT GAGTTTTTAA GTATTTGGGG CTCATCAAAT AGTAAAAATA TCTCACGCAT TCACCGTATT GAAGGTGAAC ATTTTTTTCA TGAAGCATTG GCTGCAAAAA AAGGTGTAGT TCTTATTGTT CCACATTTTG GTACGTGGGA AGTCATGAAT GCATGGTGTG CTCAATTCAC ATCAATGACC ATTTTATATA AACCCGTTAA AAATGCTGAT GCAGACCGTT TTGTGCGAGA AGCCCGTAGC CGTGAACAAG CGAATCTCGT CCCTACCGAT GAAAGCGGCG TAAGACAAAT TTTTAAAGCA TTAAAACAAG GTGAAACGAC AGTAATTTTG CCGGATCATA CCCCTAATGT GGGTGGAGAT ATGGTGAACT ATTTTGGTGT TCCACTTGCC TCAAGTAATT TAAGCGCAAA ACTCATCCAG AAAACTAAAG CAAAAGCTCT GTTTCTTTAT GCGATCCGTA ATGAAAACGA TGGCTTTACC ATGCATATCG AGCCAATGGA TGAGAAAATC TATGAGGGTA CTGCGGACGA TGGAACGTAT GTCATCCATC AAGCGATTGA GCAACTCATT TATCAATATC CAGAACATTA TCACTGGAGC TATAAACGTT TTAAAGCGAA CCCTGCTTTA GACAATATCT ATAATATCGA TCCGACAGAA GCACTTAAAA TTGTGGATAG GCTCAAAGCA GAAGCTTTAA AAACCTCTAC TCAACCAGAG CCTATCCAAA CGTCGCTCAT GTAA

Details for AcbaC.19017.a.B1.GE39037

HARVESTED ON:	data unavailable
SEQUENCED ON:	3/27/2015
EXPECTED MW:	38kDa
OBSERVED MW:	16kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	99

Validated AA Sequence

MAHHHHHHMT RPDSKSMNYQ LLKTFSRQPI QFGRFLARLL AGLVNTLKIT RTSKSIELNL RIALPYLTPQ QRIAITEKAV RNELTSYFEF LSIWGSSNSK NISRIHRIEG EHFFHEALAA KKGVVLIVPH FGTWEVMNAW CAQFTSMTIL YKPVKNADAD RFVREARSRE QANLVPTDES GVRQIFKALK QGETTVILPD HTPNVGGDMV NYFGVPLASS NLSAKLIQKT KAKALFLYAI RNENDGFTMH IEPMDEKIYE GTADDGTYVI HQAIEQLIYQ YPEHYHWSYK RFKANPALDN IYNIDPTEAL KIVDRLKAEA LKTSTQPEPI QTSL

Validated NT Sequence

catgagcgac gtttggatag gctctggttg agtagaggtt tttaaagctt ctgctttgag cctatccaca attttaagtg cttctgtcgg atcgatatta tagatattgt ctaaagcagg gttcgcttta aaacgtttat agctccagtg ataatgttct ggatattgat aaatgagttg ctcaatcgct tgatggatga catacgttcc atcgtccgca gtaccctcat agattttctc atccattggc tcgatatgca tggtaaagcc atcgttttca ttacggatcg cataaagaaa cagagctttt gctttagttt tctggatgag ttttgcgctt aaattacttg aggcaagtgg aacaccaaaa tagttcacca tatctccacc cacattaggg gtatgatccg gcaaaattac tgtcgtttca ccttgtttta atgctttaaa aatttgtctt acgccgcttt catcggtagg gacgagattc gcttgttcac ggctacgggc ttctcgcaca aaacggtctg catcagcatt tttaacgggt ttatataaaa tggtcattga tgtgaattga gcacaccatg cattcatgac ttcccacgta ccaaaatgtg gaacaataag aactacacct ttttttgcag ccaatgcttc atgaaaaaaa tgttcacctt caatacggtg aatgcgtgag atatttttac tatttgatga gccccaaata cttaaaaact caaaatatga agttaattcg tttcttacag ctttttctgt aatcgctata cgctgctgag gtgttaaata cggaagcgca attcttaagt taagctcaat actttttgat gttctagtaa tttttagagt atttacaagt cctgctaaca gacgagcaag aaaacgccca aattgaatgg gttggcggct aaatgttttg agtaattgat agttcatgct ttttgaatct ggtctagtca tatggtggtg gtggtggtga gccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacta gaccagattc aaaaagcatg aactatcaat tactcaaaac atttagccgc caacccattc aatttgggcg ttttcttgct cgtctgttag caggacttgt aaatactcta aaaattacta gaacatcaaa aagtattgag cttaacttaa gaattgcgct tccgtattta acacctcagc agcgtatagc gattacagaa aaagctgtaa gaaacgaatt aacttcatat tttgagtttt taagtatttg gggctcatca aatagtaaaa atatctcacg cattcaccgt attgaaggtg aacatttttt tcatgaagca ttggctgcaa aaaaaggtgt agttcttatt gttccacatt ttggtacgtg ggaagtcatg aatgcatggt gtgctcaatt cacatcaatg accattttat ataaacccgt taaaaatgct gatgcagacc gttttgtgcg agaagcccgt agccgtgaac aagcgaatct cgtccctacc gatgaaagcg gcgtaagaca aatttttaaa gcattaaaac aaggtgaaac gacagtaatt ttgccggatc atacccctaa tgtgggtgga gatatggtga actattttgg tgttccactt gcctcaagta atttaagcgc aaaactcatc cagaaaacta aagcaaaagc tctgtttctt tatgcgatcc gtaatgaaaa cgatggcttt accatgcata tcgagccaat ggatgagaaa atctatgagg gtactgcgga cgatggaacg tatgtcatcc atcaagcgat tgagcaactc atttatcaat atccagaaca ttatcactgg agctataaac gttttaaagc gaaccctgct ttagacaata tctataatat cgatccgaca gaagcactta aaattgtgga taggctcaaa gcagaagctt taaaaacctc tactcaacca gagcctatcc aaacgtcgct catgtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat