SSGCID: Target Page

AcbaC.19005.a

hypothetical protein

CENTER ID:	AcbaC.19005.a
ORGANISM:	Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AcbaC.19005.a.B2.GE38957	soluble domain		152	429

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AcbaC.19005.a.B2.PW37759	soluble domain		152	429

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|470.1345.peg.92
GenBank:	AKA29891.1
PGAT:	acinetobacter:ABUW_0096
RefSeq:	WP_000561449.1
UniProt:	A0A0D5YDR9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

LIAPRQGLFA SLLIISFALH TFLLVIATTH QLNENRASQG QLMTSQLVAD SLSELEPANT VSLALIANRY ATNPSVASIR ILDANKQVLA TSGMSKTRQG EIFVRDALQN EKKVGSIEIT LIQPSIGEIL RTQWLAILAS LFLHVLLWLA YRAIARPTRS EYLARINEEN RLKHEIQELT QALALEKQNT VTLVAQAQQQ AKAKPIVRSQ PEKSLESTDQ NTLALNIQFY DPKQLLSSVN QSVSVPYFKL CQLFLNKSIE LCTKHYHLKA NDIDVVDEFH AEGATLAIST SHPHAVECLL MVGTVFQLLS DVLYKRYRED KRFALQTRSA VCNAVEAMQI DAKEAAQRLA QHLHAKESAL YLDNEQLKAI QDSYQLVAMP NPSNVMTRHA FMINGMNAEC AELAQNIRTE ILMGKKSIPQ NDSPSSAAS

NT Sequence

TTGATTGCGC CTAGACAAGG GCTATTTGCT AGCCTACTGA TCATTAGTTT TGCATTGCAT ACCTTTTTAT TGGTGATTGC AACGACACAC CAGCTCAATG AAAACCGTGC GAGTCAAGGT CAGCTCATGA CCAGTCAACT CGTTGCAGAC AGCTTATCTG AGCTTGAACC TGCCAATACG GTCTCTTTAG CTCTGATTGC CAATCGCTAT GCGACCAATC CAAGTGTGGC ATCTATCCGT ATTCTTGACG CCAATAAACA AGTTTTGGCA ACAAGCGGCA TGTCAAAAAC TCGCCAAGGT GAAATTTTTG TTCGTGATGC GCTTCAAAAC GAGAAAAAAG TCGGTTCTAT TGAAATCACA CTAATTCAAC CAAGTATTGG GGAAATTCTT CGTACCCAAT GGCTTGCGAT TTTAGCTTCA CTGTTTTTAC ATGTTTTATT ATGGCTGGCT TACCGCGCCA TTGCTCGCCC AACGCGTAGC GAATACTTAG CCCGTATTAA CGAAGAAAAC CGTTTAAAAC ATGAGATTCA AGAGTTAACT CAAGCTCTAG CACTGGAAAA ACAAAATACG GTAACCTTGG TAGCTCAAGC TCAGCAGCAA GCTAAAGCTA AACCAATTGT TCGCTCACAA CCAGAAAAAA GCTTAGAGTC TACAGACCAA AATACCTTGG CACTTAATAT TCAATTTTAT GACCCTAAAC AGTTATTAAG TAGTGTTAAC CAGTCTGTTT CTGTACCGTA CTTTAAACTT TGTCAGCTCT TTTTAAATAA GAGTATTGAG CTATGTACAA AGCATTATCA TTTGAAAGCG AATGATATTG ATGTCGTAGA CGAGTTTCAT GCAGAGGGCG CAACACTTGC CATTTCGACT TCACATCCAC ACGCTGTAGA ATGCCTCCTT ATGGTAGGTA CAGTTTTCCA ATTGCTTTCG GACGTTTTAT ATAAGCGTTA CCGTGAAGAT AAACGCTTTG CGCTACAAAC TCGCAGTGCC GTGTGTAATG CCGTAGAAGC AATGCAAATT GATGCCAAAG AGGCTGCTCA ACGTTTAGCG CAACATCTTC ATGCGAAAGA ATCTGCACTC TACCTTGATA ATGAACAGCT TAAAGCAATT CAAGACAGCT ATCAGCTTGT TGCAATGCCT AACCCAAGTA ATGTCATGAC TCGTCATGCA TTTATGATCA ATGGTATGAA TGCTGAATGT GCAGAGCTTG CACAAAATAT TCGAACTGAA ATTTTGATGG GTAAAAAATC AATTCCTCAA AATGACAGCC CGAGTAGTGC AGCATCTTAA

Details for AcbaC.19005.a.B2.GE38957

HARVESTED ON:	3/4/2015
SEQUENCED ON:	3/4/2015
EXPECTED MW:	32kDa
OBSERVED MW:	32kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMR AIARPTRSEY LARINEENRL KHEIQELTQA LALEKQNTVT LVAQAQQQAK AKPIVRSQPE KSLESTDQNT LALNIQFYDP KQLLSSVNQS VSVPYFKLCQ LFLNKSIELC TKHYHLKAND IDVVDEFHAE GATLAISTSH PHAVECLLMV GTVFQLLSDV LYKRYREDKR FALQTRSAVC NAVEAMQIDA KEAAQRLAQH LHAKESALYL DNEQLKAIQD SYQLVAMPNP SNVMTRHAFM INGMNAECAE LAQNIRTEIL MGKKSIPQND SPSSAAS

Validated NT Sequence

atcttactca cttaagatgc tgcactactc gggctgtcat tttgaggaat tgatttttta cccatcaaaa tttcagttcg aatattttgt gcaagctctg cacattcagc attcatacca ttgatcataa atgcatgacg agtcatgaca ttacttgggt taggcattgc aacaagctga tagctgtctt gaattgcttt aagctgttca ttatcaaggt agagtgcaga ttctttcgca tgaagatgtt gcgctaaacg ttgagcagcc tctttggcat caatttgcat tgcttctacg gcattacaca cggcactgcg agtttgtagc gcaaagcgtt tatcttcacg gtaacgctta tataaaacgt ccgaaagcaa ttggaaaact gtacctacca taaggaggca ttctacagcg tgtggatgtg aagtcgaaat ggcaagtgtt gcgccctctg catgaaactc gtctacgaca tcaatatcat tcgctttcaa atgataatgc tttgtacata gctcaatact cttatttaaa aagagctgac aaagtttaaa gtacggtaca gaaacagact ggttaacact acttaataac tgtttagggt cataaaattg aatattaagt gccaaggtat tttggtctgt agactctaag cttttttctg gttgtgagcg aacaattggt ttagctttag cttgctgctg agcttgagct accaaggtta ccgtattttg tttttccagt gctagagctt gagttaactc ttgaatctca tgttttaaac ggttttcttc gttaatacgg gctaagtatt cgctacgcgt tgggcgagca atggcgcgca tatggtggtg gtggtggtga gccat

Expected Protein Sequence

MAHHHHHHRA IARPTRSEYL ARINEENRLK HEIQELTQAL ALEKQNTVTL VAQAQQQAKA KPIVRSQPEK SLESTDQNTL ALNIQFYDPK QLLSSVNQSV SVPYFKLCQL FLNKSIELCT KHYHLKANDI DVVDEFHAEG ATLAISTSHP HAVECLLMVG TVFQLLSDVL YKRYREDKRF ALQTRSAVCN AVEAMQIDAK EAAQRLAQHL HAKESALYLD NEQLKAIQDS YQLVAMPNPS NVMTRHAFMI NGMNAECAEL AQNIRTEILM GKKSIPQNDS PSSAAS

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcgcgcca ttgctcgccc aacgcgtagc gaatacttag cccgtattaa cgaagaaaac cgtttaaaac atgagattca agagttaact caagctctag cactggaaaa acaaaatacg gtaaccttgg tagctcaagc tcagcagcaa gctaaagcta aaccaattgt tcgctcacaa ccagaaaaaa gcttagagtc tacagaccaa aataccttgg cacttaatat tcaattttat gaccctaaac agttattaag tagtgttaac cagtctgttt ctgtaccgta ctttaaactt tgtcagctct ttttaaataa gagtattgag ctatgtacaa agcattatca tttgaaagcg aatgatattg atgtcgtaga cgagtttcat gcagagggcg caacacttgc catttcgact tcacatccac acgctgtaga atgcctcctt atggtaggta cagttttcca attgctttcg gacgttttat ataagcgtta ccgtgaagat aaacgctttg cgctacaaac tcgcagtgcc gtgtgtaatg ccgtagaagc aatgcaaatt gatgccaaag aggctgctca acgtttagcg caacatcttc atgcgaaaga atctgcactc taccttgata atgaacagct taaagcaatt caagacagct atcagcttgt tgcaatgcct aacccaagta atgtcatgac tcgtcatgca tttatgatca atggtatgaa tgctgaatgt gcagagcttg cacaaaatat tcgaactgaa attttgatgg gtaaaaaatc aattcctcaa aatgacagcc cgagtagtgc agcatcttga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at

Details for AcbaC.19005.a.B2.PW37759

PURIFICATION DATe:	8/3/2015
CONCENTRATION:	23mg/ml
OBSERVED MW:	34kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	9
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATCGCGCCA TTGCTCGCCC AACGCGTAGC GAATACTTAG CCCGTATTAA CGAAGAAAAC CGTTTAAAAC ATGAGATTCA AGAGTTAACT CAAGCTCTAG CACTGGAAAA ACAAAATACG GTAACCTTGG TAGCTCAAGC TCAGCAGCAA GCTAAAGCTA AACCAATTGT TCGCTCACAA CCAGAAAAAA GCTTAGAGTC TACAGACCAA AATACCTTGG CACTTAATAT TCAATTTTAT GACCCTAAAC AGTTATTAAG TAGTGTTAAC CAGTCTGTTT CTGTACCGTA CTTTAAACTT TGTCAGCTCT TTTTAAATAA GAGTATTGAG CTATGTACAA AGCATTATCA TTTGAAAGCG AATGATATTG ATGTCGTAGA CGAGTTTCAT GCAGAGGGCG CAACACTTGC CATTTCGACT TCACATCCAC ACGCTGTAGA ATGCCTCCTT ATGGTAGGTA CAGTTTTCCA ATTGCTTTCG GACGTTTTAT ATAAGCGTTA CCGTGAAGAT AAACGCTTTG CGCTACAAAC TCGCAGTGCC GTGTGTAATG CCGTAGAAGC AATGCAAATT GATGCCAAAG AGGCTGCTCA ACGTTTAGCG CAACATCTTC ATGCGAAAGA ATCTGCACTC TACCTTGATA ATGAACAGCT TAAAGCAATT CAAGACAGCT ATCAGCTTGT TGCAATGCCT AACCCAAGTA ATGTCATGAC TCGTCATGCA TTTATGATCA ATGGTATGAA TGCTGAATGT GCAGAGCTTG CACAAAATAT TCGAACTGAA ATTTTGATGG GTAAAAAATC AATTCCTCAA AATGACAGCC CGAGTAGTGC AGCATCTTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT