AcbaC.18998.a

NADH dehydrogenase I chain F

CENTER ID: AcbaC.18998.a
ORGANISM: Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AcbaC.18998.a.B1.GE38950 full length 1 443
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|470.1345.peg.3118
GenBank: AKA32880.1
PGAT: acinetobacter:ABUW_3173
RefSeq: WP_001091098.1
UniProt: V5VGJ0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNTEPKPIYG DGNPETHPLT WRLSKQESVR SADDYEALEG YAGFKKALGM KPAEVLEVIK AATVKGRGGA GFPAGIKWSL MAPNDGGPRY LICNADEMEP GTFKDRLLME KLPHQLIEGM LIAGYTLEAT QGYIFIRGEY IEAAQYLNEA LEQIRAKGYL GNNILGSGWN FELHVHTGAG RYICGEETAL INSLEGRRAN PRTKPPFPQV AGAWGRPTIV NNVETYNNLP AIMLRGPEWY IGLSAGKSKD PGTKIYGASG KVKFPGLWEL PFGTTAREVI EEHAGGMRDG LKLKAWLPGG ASTDFLPADT IDLPMDAETI MKAGSRLGTC LLMVVDETQC MVSLTRNLEE FFARESCGWC TPCRDGLPWA VKALKALETG EGKKEDIDHL QELTRKLWIG KTFCAHAPGA MEPLMGALKH FRSEFEAKVT DRPVVQTSNV EQA
NT Sequence
ATGAATACTG AACCAAAACC AATTTATGGC GACGGTAATC CGGAAACTCA TCCACTGACT TGGCGTTTAA GCAAACAAGA ATCAGTACGT AGTGCTGACG ATTACGAAGC ACTTGAAGGT TATGCCGGCT TTAAAAAAGC ATTAGGTATG AAACCTGCTG AAGTGCTTGA AGTCATTAAA GCTGCAACTG TAAAAGGTCG TGGTGGTGCA GGCTTCCCTG CAGGGATCAA ATGGTCTCTT ATGGCGCCAA ATGATGGCGG TCCACGTTAC TTGATCTGTA ACGCCGATGA GATGGAACCA GGTACCTTTA AAGACCGTTT GTTGATGGAA AAACTTCCTC ACCAATTGAT CGAAGGTATG TTGATTGCAG GTTATACCCT AGAAGCAACT CAAGGTTATA TCTTTATTCG TGGTGAATAT ATCGAGGCTG CACAATACCT AAATGAAGCG CTTGAGCAAA TCCGCGCTAA AGGTTACTTA GGTAACAACA TTTTAGGTTC TGGCTGGAAC TTCGAGTTAC ACGTGCACAC CGGTGCAGGT CGTTATATTT GTGGTGAAGA AACTGCATTG ATTAACTCAC TTGAAGGTCG CCGTGCTAAC CCACGTACTA AACCACCATT CCCGCAAGTT GCTGGTGCAT GGGGTCGTCC AACGATTGTG AACAACGTTG AGACTTATAA CAACTTGCCA GCAATCATGC TTCGTGGCCC AGAATGGTAT ATCGGTTTAT CAGCAGGTAA ATCAAAAGAT CCGGGCACAA AGATTTATGG TGCGTCAGGT AAAGTAAAAT TCCCTGGTCT TTGGGAACTA CCATTTGGTA CGACTGCACG TGAAGTAATC GAAGAACATG CTGGTGGTAT GCGTGATGGC TTGAAGCTTA AAGCATGGTT ACCAGGCGGT GCGTCTACTG ACTTTTTACC GGCGGATACC ATTGATTTAC CAATGGATGC TGAAACCATT ATGAAAGCAG GTTCTCGTTT AGGAACATGT TTATTAATGG TGGTAGACGA GACCCAATGT ATGGTGTCGT TGACCCGTAA TCTGGAAGAA TTCTTTGCGC GTGAATCATG TGGCTGGTGT ACACCTTGCC GTGATGGTTT GCCATGGGCT GTTAAAGCGC TTAAAGCACT TGAGACAGGT GAAGGTAAGA AAGAGGATAT CGACCACTTA CAAGAACTTA CTCGTAAATT GTGGATTGGT AAAACTTTCT GTGCCCACGC ACCAGGTGCG ATGGAGCCGC TTATGGGCGC ACTCAAACAT TTCCGTAGCG AGTTTGAAGC GAAAGTGACT GATCGTCCTG TCGTGCAAAC TAGCAACGTA GAACAAGCTT AA
Details for AcbaC.18998.a.B1.GE38950
HARVESTED ON: 3/4/2015
SEQUENCED ON: 3/4/2015
EXPECTED MW: 50kDa
OBSERVED MW: 47kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMN TEPKPIYGDG NPETHPLTWR LSKQESVRSA DDYEALEGYA GFKKALGMKP AEVLEVIKAA TVKGRGGAGF PAGIKWSLMA PNDGGPRYLI CNADEMEPGT FKDRLLMEKL PHQLIEGMLI AGYTLEATQG YIFIRGEYIE AAQYLNEALE QIRAKGYLGN NILGSGWNFE LHVHTGAGRY ICGEETALIN SLEGRRANPR TKPPFPQVAG AWGRPTIVNN VETYNNLPAI MLRGPEWYIG LSAGKSKDPG TKIYGASGKV KFPGLWELPF GTTAREVIEE HAGGMRDGLK LKAWLPGGAS TDFLPADTID LPMDAETIMK AGSRLGTCLL MVVDETQCMV SLTRNLEEFF ARESCGWCTP CRDGLPWAVK ALKALETGEG KKEDIDHLQE LTRKLWIGKT FCAHAPGAME PLMGALKHFR SEFEAKVTDR PVV
Validated NT Sequence
tgcacgacag gacgatcagt cactttcgct tcaaactcgc tacggaaatg tttgagtgcg cccataagcg gctccatcgc acctggtgcg tgggcacaga aagttttacc aatccacaat ttacgagtaa gttcttgtaa gtggtcgata tcctctttct taccttcacc tgtctcaagt gctttaagcg ctttaacagc ccatggcaaa ccatcacggc aaggtgtaca ccagccacat gattcacgcg caaagaattc ttccagatta cgggtcaacg acaccataca ttgggtctcg tctaccacca ttaataaaca tgttcctaaa cgagaacctg ctttcataat ggtttcagca tccattggta aatcaatggt atccgccggt aaaaagtcag tagacgcacc gcctggtaac catgctttaa gcttcaagcc atcacgcata ccaccagcat gttcttcgat tacttcacgt gcagtcgtac caaatggtag ttcccaaaga ccagggaatt ttactttacc tgacgcacca taaatctttg tgcccggatc ttttgattta cctgctgata aaccgatata ccattctggg ccacgaagca tgattgctgg caagttgtta taagtctcaa cgttgttcac aatcgttgga cgaccccatg caccagcaac ttgcgggaat ggtggtttag tacgtgggtt agcacggcga ccttcaagtg agttaatcaa tgcagtttct tcaccacaaa tataacgacc tgcaccggtg tgcacgtgta actcgaagtt ccagccagaa cctaaaatgt tgttacctaa gtaaccttta gcgcggattt gctcaagcgc ttcatttagg tattgtgcag cctcgatata ttcaccacga ataaagatat aaccttgagt tgcttctagg gtataacctg caatcaacat accttcgatc aattggtgag gaagtttttc catcaacaaa cggtctttaa aggtacctgg ttccatctca tcggcgttac agatcaagta acgtggaccg ccatcatttg gcgccataag agaccatttg atccctgcag ggaagcctgc accaccacga ccttttacag ttgcagcttt aatgacttca agcacttcag caggtttcat acctaatgct tttttaaagc cggcataacc ttcaagtgct tcgtaatcgt cagcactacg tactgattct tgtttgctta aacgccaagt cagtggatga gtttccggat taccgtcgcc ataaattggt tttggttcag tattcatatg gtggtggtgg tggtgagcca t
Expected Protein Sequence
MAHHHHHHMN TEPKPIYGDG NPETHPLTWR LSKQESVRSA DDYEALEGYA GFKKALGMKP AEVLEVIKAA TVKGRGGAGF PAGIKWSLMA PNDGGPRYLI CNADEMEPGT FKDRLLMEKL PHQLIEGMLI AGYTLEATQG YIFIRGEYIE AAQYLNEALE QIRAKGYLGN NILGSGWNFE LHVHTGAGRY ICGEETALIN SLEGRRANPR TKPPFPQVAG AWGRPTIVNN VETYNNLPAI MLRGPEWYIG LSAGKSKDPG TKIYGASGKV KFPGLWELPF GTTAREVIEE HAGGMRDGLK LKAWLPGGAS TDFLPADTID LPMDAETIMK AGSRLGTCLL MVVDETQCMV SLTRNLEEFF ARESCGWCTP CRDGLPWAVK ALKALETGEG KKEDIDHLQE LTRKLWIGKT FCAHAPGAME PLMGALKHFR SEFEAKVTDR PVVQTSNVEQ A
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaata ctgaaccaaa accaatttat ggcgacggta atccggaaac tcatccactg acttggcgtt taagcaaaca agaatcagta cgtagtgctg acgattacga agcacttgaa ggttatgccg gctttaaaaa agcattaggt atgaaacctg ctgaagtgct tgaagtcatt aaagctgcaa ctgtaaaagg tcgtggtggt gcaggcttcc ctgcagggat caaatggtct cttatggcgc caaatgatgg cggtccacgt tacttgatct gtaacgccga tgagatggaa ccaggtacct ttaaagaccg tttgttgatg gaaaaacttc ctcaccaatt gatcgaaggt atgttgattg caggttatac cctagaagca actcaaggtt atatctttat tcgtggtgaa tatatcgagg ctgcacaata cctaaatgaa gcgcttgagc aaatccgcgc taaaggttac ttaggtaaca acattttagg ttctggctgg aacttcgagt tacacgtgca caccggtgca ggtcgttata tttgtggtga agaaactgca ttgattaact cacttgaagg tcgccgtgct aacccacgta ctaaaccacc attcccgcaa gttgctggtg catggggtcg tccaacgatt gtgaacaacg ttgagactta taacaacttg ccagcaatca tgcttcgtgg cccagaatgg tatatcggtt tatcagcagg taaatcaaaa gatccgggca caaagattta tggtgcgtca ggtaaagtaa aattccctgg tctttgggaa ctaccatttg gtacgactgc acgtgaagta atcgaagaac atgctggtgg tatgcgtgat ggcttgaagc ttaaagcatg gttaccaggc ggtgcgtcta ctgacttttt accggcggat accattgatt taccaatgga tgctgaaacc attatgaaag caggttctcg tttaggaaca tgtttattaa tggtggtaga cgagacccaa tgtatggtgt cgttgacccg taatctggaa gaattctttg cgcgtgaatc atgtggctgg tgtacacctt gccgtgatgg tttgccatgg gctgttaaag cgcttaaagc acttgagaca ggtgaaggta agaaagagga tatcgaccac ttacaagaac ttactcgtaa attgtggatt ggtaaaactt tctgtgccca cgcaccaggt gcgatggagc cgcttatggg cgcactcaaa catttccgta gcgagtttga agcgaaagtg actgatcgtc ctgtcgtgca aactagcaac gtagaacaag cttgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat