SSGCID: Target Page

AcbaC.18996.a

DNA polymerase I

CENTER ID:	AcbaC.18996.a
ORGANISM:	Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AcbaC.18996.a.B1.GE38948	full length		1	923

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AcbaC.18996.a.B1.PS02434	full length		1	923

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|470.1345.peg.3204
GenBank:	AKA32966.1
PGAT:	acinetobacter:ABUW_3270
RefSeq:	WP_001134663.1
UniProt:	A0A081H8P8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPPFVLVDGS YFLFRAYHAL PPLTTSTGLH TNAIRGAISA IQKLMRRMQP THMAVIFDTP EPTFRHKLSP IYKGDRPTMP EELSQQIPYL HALIKAQGIP LYSLPGAEAD DIIGTLAKRA VKEGHHVLIS TGDKDMAQLV NDYVKLEDSF KDRVLDTDGV FEKFGVWPNQ IIDYLTLMGD ASDGIMGVPG VGAKTAAKLL TEYGTLDNII ANVDQLKGKL SQNIKDNLDN IKLDHQLASI VCDLPLELDW HELKLTDPNV DALRNLYTEL EFRNQLQSLD HPNNPNSSSY KQQASQTIVQ NEVKPAQTIE TEDQASLTSQ DDQLGTANYH TVLTQEAWDQ LWQRMQNADH FAIDTETTSL DYRIAEMVGF SIAFDAKDAY YVPLAHNYEN APQQLDREQV LAQIKPVLEN EAVKKIGHHL KYDAHIFANH GIELKGWYFD SMLASYVLNA AATRHGMDDV ARVYLSHLTT TFEQIAGKGA KQKTFNQIEI EVAAHYAAED AHVTYRLYEV LSNKLKAHPE LENILYNIEM PVARILSGME EDGIRLDHAF LDKLSVEFAK TMEDLENQAM EIAGETFNIA SPKQVGEILF DKLGIKGGKK TATGQYSTSE SVLEKIEHPL TEIILEHRGL AKLKSTYTDR LIEQSHNDTH RVHTSYHQAL TATGRLSSSD PNLQNIPIRR QIGRQIRKAF IAPEGRVLLA ADYSQIELRL MAHFSQDDAL VHAFQHGQDV HRRTAAEVLG IAIEDVTNDQ RRQAKAVNFG LLYGMSEFGL IRQLGFTREE SQNYIKQYFQ RYPGIYEYMQ RTRQVASEQG FVETILGRRL YTPDIDARNV MVRKAAERAA INAPLQGSAA DIIKLAMIEV DKILPKDQAK LLLQVHDELV FEVDSNIADE LSKQIADVMQ SVLEISVPLV VEVGQGKNWD DAH

NT Sequence

ATGCCACCAT TTGTCTTGGT TGATGGGTCC TATTTTTTAT TTCGTGCATA TCATGCATTA CCACCATTGA CGACCTCTAC AGGGTTACAT ACCAATGCAA TACGTGGAGC AATTTCTGCC ATTCAAAAAT TGATGCGTCG TATGCAACCG ACACATATGG CTGTCATTTT TGATACGCCT GAGCCAACTT TCCGTCATAA GCTTTCACCT ATTTATAAAG GTGACCGCCC GACTATGCCG GAAGAATTGT CTCAACAGAT TCCATATTTA CATGCATTGA TTAAGGCTCA AGGGATTCCT TTATATAGTC TTCCGGGTGC AGAAGCCGAT GACATTATCG GGACTCTCGC TAAACGCGCC GTAAAAGAAG GTCATCATGT GCTTATCTCA ACCGGAGATA AAGACATGGC CCAGCTGGTT AATGACTATG TAAAACTTGA AGATAGCTTT AAAGATCGAG TATTAGACAC CGACGGCGTA TTTGAAAAAT TTGGCGTTTG GCCAAACCAG ATTATCGATT ACTTAACCCT TATGGGTGAT GCATCTGACG GCATTATGGG TGTACCTGGC GTAGGCGCAA AGACGGCTGC CAAATTACTG ACTGAATATG GGACATTGGA TAATATCATT GCCAATGTAG ACCAACTTAA AGGCAAACTG AGCCAAAATA TTAAAGATAA TTTAGACAAT ATTAAGCTTG ATCACCAGTT GGCAAGCATT GTCTGTGACC TTCCTTTAGA ACTCGACTGG CATGAATTAA AACTTACTGA TCCAAATGTA GATGCCTTAC GCAATCTCTA TACAGAGCTT GAGTTTAGAA ACCAGTTACA GTCTCTCGAT CATCCAAACA ATCCAAATAG CTCAAGCTAT AAGCAGCAAG CTTCACAAAC TATTGTCCAA AATGAAGTTA AACCTGCTCA AACGATTGAA ACAGAAGATC AGGCAAGCTT GACTAGTCAA GATGACCAGC TTGGAACTGC AAACTATCAT ACTGTTTTAA CACAAGAAGC ATGGGATCAG CTCTGGCAAC GCATGCAGAA TGCCGATCAT TTTGCTATTG ATACAGAGAC CACAAGTCTT GATTACCGTA TTGCCGAGAT GGTTGGTTTT TCAATCGCAT TTGATGCGAA AGATGCCTAC TATGTTCCTT TAGCACATAA TTACGAAAAT GCGCCACAAC AACTGGACCG TGAACAAGTT TTAGCGCAAA TCAAGCCTGT ACTTGAAAAT GAAGCAGTTA AAAAAATCGG TCATCATCTT AAATATGATG CGCACATTTT TGCTAATCAC GGTATTGAAC TTAAAGGTTG GTATTTCGAT AGCATGCTGG CATCTTATGT ACTGAATGCA GCAGCTACTC GTCATGGCAT GGATGATGTT GCTCGTGTTT ACCTAAGCCA TTTGACCACT ACTTTTGAGC AAATTGCTGG CAAAGGTGCA AAACAGAAGA CTTTTAATCA AATTGAGATT GAAGTTGCAG CTCATTATGC AGCAGAAGAT GCACATGTGA CCTATCGTTT ATATGAAGTA CTTTCAAATA AACTTAAAGC ACATCCTGAA TTGGAAAATA TTTTATATAA CATTGAGATG CCAGTTGCCC GAATCTTATC GGGTATGGAG GAAGATGGAA TCCGTTTAGA TCATGCTTTC TTAGATAAAT TAAGTGTTGA ATTTGCGAAA ACTATGGAAG ACCTTGAAAA TCAGGCCATG GAAATTGCAG GTGAAACTTT TAATATTGCT TCGCCTAAAC AAGTAGGTGA AATTCTATTT GATAAGTTAG GCATTAAAGG TGGTAAAAAA ACTGCAACTG GGCAATACAG CACCAGTGAA AGTGTTCTTG AAAAAATTGA GCATCCCCTA ACAGAAATTA TTTTAGAACA CCGTGGATTA GCAAAATTAA AGAGCACCTA TACTGACCGT TTAATTGAGC AGTCGCATAA TGACACCCAT CGTGTACATA CCAGCTATCA TCAAGCACTA ACGGCAACAG GTCGCCTATC CTCTTCTGAC CCTAACTTAC AAAACATTCC AATTCGTAGA CAGATTGGCC GTCAAATCCG TAAAGCATTT ATTGCACCGG AAGGACGAGT TTTGTTAGCA GCCGATTACT CGCAAATTGA ACTTCGTTTA ATGGCTCATT TTTCTCAAGA TGATGCCTTA GTTCATGCAT TCCAACATGG TCAAGATGTA CACCGTAGAA CAGCGGCAGA AGTGTTAGGT ATTGCAATTG AAGATGTGAC CAATGATCAG CGCCGTCAGG CTAAAGCTGT GAATTTCGGT CTACTTTACG GCATGTCCGA GTTTGGTTTA ATTCGTCAAC TTGGCTTTAC TCGTGAAGAA TCGCAAAACT ATATCAAACA ATACTTCCAA CGCTATCCTG GCATTTACGA ATACATGCAA CGTACTCGCC AAGTAGCATC GGAACAAGGG TTTGTTGAAA CTATTTTAGG TCGCCGTCTT TATACACCGG ATATTGACGC TCGAAATGTG ATGGTACGTA AAGCTGCTGA ACGTGCTGCA ATTAATGCAC CTTTACAAGG TAGTGCTGCT GATATTATTA AGTTAGCAAT GATTGAAGTA GATAAAATCC TGCCAAAAGA TCAAGCAAAA CTATTACTTC AAGTTCATGA TGAATTGGTG TTTGAAGTTG ATTCAAACAT TGCAGATGAA CTTTCAAAAC AAATTGCGGA TGTCATGCAG TCTGTTCTGG AAATTTCTGT ACCGTTAGTC GTAGAAGTAG GCCAAGGTAA AAACTGGGAT GATGCCCATT AA

Details for AcbaC.18996.a.B1.GE38948

HARVESTED ON:	3/4/2015
SEQUENCED ON:	3/4/2015
EXPECTED MW:	105kDa
OBSERVED MW:	105kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMP PFVLVDGSYF LFRAYHALPP LTTSTGLHTN AIRGAISAIQ KLMRRMQPTH MAVIFDTPEP TFRHKLSPIY KGDRPTMPEE LSQQIPYLHA LIKAQGIPLY SLPGAEADDI IGTLAKRAVK EGHHVLISTG DKDMAQLVND YVKLEDSFKD RVLDTDGVFE KFGVWPNQII DYLTLMGDAS DGIMGVPGVG AKTAAKLLTE YGTLDNIIAN VDQLKGKLSQ NIKDNLDNIK LDHQLASIVC DLPLELDWHE LKLTDPNVDA LRNLYTELEF RNQLQSLDHP NNPNSSSYKQ QASQTIVQNE VKPAQTIETE DQASLTSQDD QLGTANYHTV LTQEAWDQLW QRMQNADHFA IDTETTSLDY RIAEMVGFSI AFDAKDAYYV PLAHNYENAP QQLDREQVLA QIKPVLENEA VKKIGHHLKY DAHIFANHGI ELKGWYFDSM LASYVLNAAA TRHGMDDVAR VYLSHLTTTF EQIAGKGAKQ KTFNQIEIEV AAHYAAEDAH VTYRLYEVLS NKLKAHPELE NILYNIEMPV ARILSGMEED GIRLDHAFLD KLSVEFAKTM EDLENQAMEI AGETFNIASP KQVGEILFDK LGIKGGKKTA TGQYSTSESV LEKIEHPLTE IILEHRGLAK LKSTYTDRLI EQSHNDTHRV HTSYHQALTA TGRLSSSDPN LQNIPIRRQI GRQIRKAFIA PEGRVLLAAD YSQIELRLMA HFSQDDALVH AFQHGQDVHR RTAAEVLGIA IEDVTNDQRR QAKAVNFGLL YGMSEFGLIR QLGFTREESQ NYIKQYFQRY PGIYEYMQRT RQVASEQGFV ETILGRRLYT PDIDARNVMV RKAAERAAIN APLQGSAADI IKLAMIEVDK ILPKDQAKLL LQVHDELVFE VDSNIADELS KQIADVMQSV LEISVPLVVE VGQGKNWDDA H

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgccac catttgtctt ggttgatggg tcctattttt tatttcgtgc atatcatgca ttaccaccat tgacgacctc tacagggtta cataccaatg caatacgtgg agcaatttct gccattcaaa aattgatgcg tcgtatgcaa ccgacacata tggctgtcat ttttgatacg cctgagccaa ctttccgtca taagctttca cctatttata aaggtgaccg cccgactatg ccggaagaat tgtctcaaca gattccatat ttacatgcat tgattaaggc tcaagggatt cctttatata gtcttccggg tgcagaagcc gatgacatta tcgggactct cgctaaacgc gccgtaaaag aaggtcatca tgtgcttatc tcaaccggag ataaagacat ggcccagctg gttaatgact atgtaaaact tgaagatagc tttaaagatc gagtattaga caccgacggc gtatttgaaa aatttggcgt ttggccaaac cagattatcg attacttaac ccttatgggt gatgcatctg acggcattat gggtgtacct ggcgtaggcg caaagacggc tgccaaatta ctgactgaat atgggacatt ggataatatc attgccaatg tagaccaact taaaggcaaa ctgagccaaa atattaaaga taatttagac aatattaagc ttgatcacca gttggcaagc attgtctgtg accttccttt agaactcgac tggcatgaat taaaacttac tgatccaaat gtagatgcct tacgcaatct ctatacagag cttgagttta gaaaccagtt acagtctctc gatcatccaa acaatccaaa tagctcaagc tataagcagc aagcttcaca aactattgtc caaaatgaag ttaaacctgc tcaaacgatt gaaacagaag atcaggcaag cttgactagt caagatgacc agcttggaac tgcaaactat catactgttt taacacaaga agcatgggat cagctctggc aacgcatgca gaatgccgat cattttgcta ttgatacaga gaccacaagt cttgattacc gtattgccga gatggttggt ttttcaatcg catttgatgc gaaagatgcc tactatgttc ctttagcaca taattacgaa aatgcgccac aacaactgga ccgtgaacaa gttttagcgc aaatcaagcc tgtacttgaa aatgaagcag ttaaaaaaat cggtcatcat cttaaatatg atgcgcacat ttttgctaat cacggtattg aacttaaagg ttggtatttc gatagcatgc tggcatctta tgtactgaat gcagcagcta ctcgtcatgg catggatgat gttgctcgtg tttacctaag ccatttgacc actacttttg agcaaattgc tggcaaaggt gcaaaacaga agacttttaa tcaaattgag attgaagttg cagctcatta tgcagcagaa gatgcacatg tgacctatcg tttatatgaa gtactttcaa ataaacttaa agcacatcct gaattggaaa atattttata taacattgag atgccagttg cccgaatctt atcgggtatg gaggaagatg gaatccgttt agatcatgct ttcttagata aattaagtgt tgaatttgcg aaaactatgg aagaccttga aaatcaggcc atggaaattg caggtgaaac ttttaatatt gcttcgccta aacaagtagg tgaaattcta tttgataagt taggcattaa aggtggtaaa aaaactgcaa ctgggcaata cagcaccagt gaaagtgttc ttgaaaaaat tgagcatccc ctaacagaaa ttattttaga acaccgtgga ttagcaaaat taaagagcac ctatactgac cgtttaattg agcagtcgca taatgacacc catcgtgtac ataccagcta tcatcaagca ctaacggcaa caggtcgcct atcctcttct gaccctaact tacaaaacat tccaattcgt agacagattg gccgtcaaat ccgtaaagca tttattgcac cggaaggacg agttttgtta gcagccgatt actcgcaaat tgaacttcgt ttaatggctc atttttctca agatgatgcc ttagttcatg cattccaaca tggtcaagat gtacaccgta gaacagcggc agaagtgtta ggtattgcaa ttgaagatgt gaccaatgat cagcgccgtc aggctaaagc tgtgaatttc ggtctacttt acggcatgtc cgagtttggt ttaattcgtc aacttggctt tactcgtgaa gaatcgcaaa actatatcaa acaatacttc caacgctatc ctggcattta cgaatacatg caacgtactc gccaagtagc atcggaacaa gggtttgttg aaactatttt aggtcgccgt ctttatacac cggatattga cgctcgaaat gtgatggtac gtaaagctgc tgaacgtgct gcaattaatg cacctttaca aggtagtgct gctgatatta ttaagttagc aatgattgaa gtagataaaa tcctgccaaa agatcaagca aaactattac ttcaagttca tgatgaattg gtgtttgaag ttgattcaaa cattgcagat gaactttcaa aacaaattgc ggatgtcatg cagtctgttc tggaaatttc tgtaccgtta gtcgtagaag taggccaagg taaaaactgg gatgatgccc attgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat

Details for AcbaC.18996.a.B1.PS02434

PURIFICATION DATe:	6/23/2015
CONCENTRATION:	38.34mg/ml
OBSERVED MW:	110kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	2
VIAL VOLUME:	110µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCCAC CATTTGTCTT GGTTGATGGG TCCTATTTTT TATTTCGTGC ATATCATGCA TTACCACCAT TGACGACCTC TACAGGGTTA CATACCAATG CAATACGTGG AGCAATTTCT GCCATTCAAA AATTGATGCG TCGTATGCAA CCGACACATA TGGCTGTCAT TTTTGATACG CCTGAGCCAA CTTTCCGTCA TAAGCTTTCA CCTATTTATA AAGGTGACCG CCCGACTATG CCGGAAGAAT TGTCTCAACA GATTCCATAT TTACATGCAT TGATTAAGGC TCAAGGGATT CCTTTATATA GTCTTCCGGG TGCAGAAGCC GATGACATTA TCGGGACTCT CGCTAAACGC GCCGTAAAAG AAGGTCATCA TGTGCTTATC TCAACCGGAG ATAAAGACAT GGCCCAGCTG GTTAATGACT ATGTAAAACT TGAAGATAGC TTTAAAGATC GAGTATTAGA CACCGACGGC GTATTTGAAA AATTTGGCGT TTGGCCAAAC CAGATTATCG ATTACTTAAC CCTTATGGGT GATGCATCTG ACGGCATTAT GGGTGTACCT GGCGTAGGCG CAAAGACGGC TGCCAAATTA CTGACTGAAT ATGGGACATT GGATAATATC ATTGCCAATG TAGACCAACT TAAAGGCAAA CTGAGCCAAA ATATTAAAGA TAATTTAGAC AATATTAAGC TTGATCACCA GTTGGCAAGC ATTGTCTGTG ACCTTCCTTT AGAACTCGAC TGGCATGAAT TAAAACTTAC TGATCCAAAT GTAGATGCCT TACGCAATCT CTATACAGAG CTTGAGTTTA GAAACCAGTT ACAGTCTCTC GATCATCCAA ACAATCCAAA TAGCTCAAGC TATAAGCAGC AAGCTTCACA AACTATTGTC CAAAATGAAG TTAAACCTGC TCAAACGATT GAAACAGAAG ATCAGGCAAG CTTGACTAGT CAAGATGACC AGCTTGGAAC TGCAAACTAT CATACTGTTT TAACACAAGA AGCATGGGAT CAGCTCTGGC AACGCATGCA GAATGCCGAT CATTTTGCTA TTGATACAGA GACCACAAGT CTTGATTACC GTATTGCCGA GATGGTTGGT TTTTCAATCG CATTTGATGC GAAAGATGCC TACTATGTTC CTTTAGCACA TAATTACGAA AATGCGCCAC AACAACTGGA CCGTGAACAA GTTTTAGCGC AAATCAAGCC TGTACTTGAA AATGAAGCAG TTAAAAAAAT CGGTCATCAT CTTAAATATG ATGCGCACAT TTTTGCTAAT CACGGTATTG AACTTAAAGG TTGGTATTTC GATAGCATGC TGGCATCTTA TGTACTGAAT GCAGCAGCTA CTCGTCATGG CATGGATGAT GTTGCTCGTG TTTACCTAAG CCATTTGACC ACTACTTTTG AGCAAATTGC TGGCAAAGGT GCAAAACAGA AGACTTTTAA TCAAATTGAG ATTGAAGTTG CAGCTCATTA TGCAGCAGAA GATGCACATG TGACCTATCG TTTATATGAA GTACTTTCAA ATAAACTTAA AGCACATCCT GAATTGGAAA ATATTTTATA TAACATTGAG ATGCCAGTTG CCCGAATCTT ATCGGGTATG GAGGAAGATG GAATCCGTTT AGATCATGCT TTCTTAGATA AATTAAGTGT TGAATTTGCG AAAACTATGG AAGACCTTGA AAATCAGGCC ATGGAAATTG CAGGTGAAAC TTTTAATATT GCTTCGCCTA AACAAGTAGG TGAAATTCTA TTTGATAAGT TAGGCATTAA AGGTGGTAAA AAAACTGCAA CTGGGCAATA CAGCACCAGT GAAAGTGTTC TTGAAAAAAT TGAGCATCCC CTAACAGAAA TTATTTTAGA ACACCGTGGA TTAGCAAAAT TAAAGAGCAC CTATACTGAC CGTTTAATTG AGCAGTCGCA TAATGACACC CATCGTGTAC ATACCAGCTA TCATCAAGCA CTAACGGCAA CAGGTCGCCT ATCCTCTTCT GACCCTAACT TACAAAACAT TCCAATTCGT AGACAGATTG GCCGTCAAAT CCGTAAAGCA TTTATTGCAC CGGAAGGACG AGTTTTGTTA GCAGCCGATT ACTCGCAAAT TGAACTTCGT TTAATGGCTC ATTTTTCTCA AGATGATGCC TTAGTTCATG CATTCCAACA TGGTCAAGAT GTACACCGTA GAACAGCGGC AGAAGTGTTA GGTATTGCAA TTGAAGATGT GACCAATGAT CAGCGCCGTC AGGCTAAAGC TGTGAATTTC GGTCTACTTT ACGGCATGTC CGAGTTTGGT TTAATTCGTC AACTTGGCTT TACTCGTGAA GAATCGCAAA ACTATATCAA ACAATACTTC CAACGCTATC CTGGCATTTA CGAATACATG CAACGTACTC GCCAAGTAGC ATCGGAACAA GGGTTTGTTG AAACTATTTT AGGTCGCCGT CTTTATACAC CGGATATTGA CGCTCGAAAT GTGATGGTAC GTAAAGCTGC TGAACGTGCT GCAATTAATG CACCTTTACA AGGTAGTGCT GCTGATATTA TTAAGTTAGC AATGATTGAA GTAGATAAAA TCCTGCCAAA AGATCAAGCA AAACTATTAC TTCAAGTTCA TGATGAATTG GTGTTTGAAG TTGATTCAAA CATTGCAGAT GAACTTTCAA AACAAATTGC GGATGTCATG CAGTCTGTTC TGGAAATTTC TGTACCGTTA GTCGTAGAAG TAGGCCAAGG TAAAAACTGG GATGATGCCC ATTGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT