SSGCID: Target Page

AcbaC.18892.a

acetyl-CoA carboxylase, carboxyl transferase, alpha subunit

CENTER ID:	AcbaC.18892.a
ORGANISM:	Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AcbaC.18892.a.B1.GE38943	full length		1	273

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|470.1345.peg.3208
GenBank:	AKA32970.1
PGAT:	acinetobacter:ABUW_3274
RefSeq:	WP_000710403.1
UniProt:	V5VGE4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKKATQSKAW TTVQIARHPE RPQFLDYVGE IFTEFDALHG DRLFGDDGAM VGGLARFDGQ PVMVIGQHRG RSTREKLKHN FGMCNPEGYR KSQRLLDMAE RFNLPVFTFI DTMGAYPGVG AEERGQAEAI ATSLAQLSSL KVPVIATVLG EGGSGGALGI GVADRVIMLS HSIYSVISPE GCASILWKTA EKAAQASEAL GLTADKLQSL GIVEYVVDEG EGAHLDPERV MQNLKVVLKQ ALDELLPMDA NERCEARYQR LMKFGSENLG MAS

NT Sequence

ATGAAAAAAG CTACTCAGTC CAAAGCGTGG ACAACGGTTC AAATTGCACG TCATCCTGAA CGTCCGCAGT TTCTAGATTA TGTCGGTGAA ATCTTTACAG AGTTTGATGC ATTGCATGGT GATCGTTTGT TTGGTGACGA TGGTGCGATG GTAGGTGGTC TTGCTCGTTT TGACGGTCAA CCTGTGATGG TCATTGGACA ACACCGTGGT CGTAGTACCC GTGAAAAATT AAAGCACAAC TTTGGTATGT GTAACCCAGA AGGTTATCGT AAATCTCAGC GCCTTTTAGA TATGGCTGAG CGTTTCAATC TACCAGTTTT CACCTTTATT GATACTATGG GTGCATATCC GGGTGTTGGT GCTGAAGAAC GTGGTCAGGC AGAAGCAATT GCTACTAGCC TTGCTCAACT TTCAAGTCTA AAAGTGCCTG TAATCGCAAC AGTACTTGGT GAAGGCGGTT CTGGTGGTGC TTTAGGTATT GGTGTTGCTG ACAGAGTGAT TATGTTGTCA CATAGTATTT ATTCTGTGAT TTCGCCTGAA GGCTGTGCAT CTATTTTGTG GAAAACTGCT GAGAAAGCAG CTCAAGCGAG TGAAGCTTTA GGTTTAACAG CTGATAAACT ACAATCGCTA GGTATTGTTG AATATGTAGT AGATGAAGGT GAAGGTGCGC ATTTAGATCC TGAGCGTGTG ATGCAAAACT TAAAAGTAGT TTTAAAGCAG GCTTTGGATG AATTGCTACC AATGGATGCA AATGAACGAT GCGAAGCACG TTATCAACGT TTAATGAAGT TTGGCAGCGA AAATTTAGGC ATGGCGTCTT AA

Details for AcbaC.18892.a.B1.GE38943

HARVESTED ON:	3/4/2015
SEQUENCED ON:	3/4/2015
EXPECTED MW:	31kDa
OBSERVED MW:	27kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMK KATQSKAWTT VQIARHPERP QFLDYVGEIF TEFDALHGDR LFGDDGAMVG GLARFDGQPV MVIGQHRGRS TREKLKHNFG MCNPEGYRKS QRLLDMAERF NLPVFTFIDT MGAYPGVGAE ERGQAEAIAT SLAQLSSLKV PVIATVLGEG GSGGALGIGV ADRVIMLSHS IYSVISPEGC ASILWKTAEK AAQASEALGL TADKLQSLGI VEYVVDEGEG AHLDPERVMQ NLKVVLKQAL DELLPMDANE RCEARYQRLM KFGSENLGMA S

Validated NT Sequence

atcttactca cttaagacgc catgcctaaa ttttcgctgc caaacttcat taaacgttga taacgtgctt cgcatcgttc atttgcatcc attggtagca attcatccaa agcctgcttt aaaactactt ttaagttttg catcacacgc tcaggatcta aatgcgcacc ttcaccttca tctactacat attcaacaat acctagcgat tgtagtttat cagctgttaa acctaaagct tcactcgctt gagctgcttt ctcagcagtt ttccacaaaa tagatgcaca gccttcaggc gaaatcacag aataaatact atgtgacaac ataatcactc tgtcagcaac accaatacct aaagcaccac cagaaccgcc ttcaccaagt actgttgcga ttacaggcac ttttagactt gaaagttgag caaggctagt agcaattgct tctgcctgac cacgttcttc agcaccaaca cccggatatg cacccatagt atcaataaag gtgaaaactg gtagattgaa acgctcagcc atatctaaaa ggcgctgaga tttacgataa ccttctgggt tacacatacc aaagttgtgc tttaattttt cacgggtact acgaccacgg tgttgtccaa tgaccatcac aggttgaccg tcaaaacgag caagaccacc taccatcgca ccatcgtcac caaacaaacg atcaccatgc aatgcatcaa actctgtaaa gatttcaccg acataatcta gaaactgcgg acgttcagga tgacgtgcaa tttgaaccgt tgtccacgct ttggactgag tagctttttt catatggtgg tggtggtggt gagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaaa aagctactca gtccaaagcg tggacaacgg ttcaaattgc acgtcatcct gaacgtccgc agtttctaga ttatgtcggt gaaatcttta cagagtttga tgcattgcat ggtgatcgtt tgtttggtga cgatggtgcg atggtaggtg gtcttgctcg ttttgacggt caacctgtga tggtcattgg acaacaccgt ggtcgtagta cccgtgaaaa attaaagcac aactttggta tgtgtaaccc agaaggttat cgtaaatctc agcgcctttt agatatggct gagcgtttca atctaccagt tttcaccttt attgatacta tgggtgcata tccgggtgtt ggtgctgaag aacgtggtca ggcagaagca attgctacta gccttgctca actttcaagt ctaaaagtgc ctgtaatcgc aacagtactt ggtgaaggcg gttctggtgg tgctttaggt attggtgttg ctgacagagt gattatgttg tcacatagta tttattctgt gatttcgcct gaaggctgtg catctatttt gtggaaaact gctgagaaag cagctcaagc gagtgaagct ttaggtttaa cagctgataa actacaatcg ctaggtattg ttgaatatgt agtagatgaa ggtgaaggtg cgcatttaga tcctgagcgt gtgatgcaaa acttaaaagt agttttaaag caggctttgg atgaattgct accaatggat gcaaatgaac gatgcgaagc acgttatcaa cgtttaatga agtttggcag cgaaaattta ggcatggcgt cttgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat