AcbaC.18890.a

phenylalanyl-tRNA synthetase, beta subunit

CENTER ID: AcbaC.18890.a
ORGANISM: Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AcbaC.18890.a.B1.GE38942 full length 1 793
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|470.1345.peg.3214
GenBank: AKA32976.1
PGAT: acinetobacter:ABUW_3280
RefSeq: WP_000703078.1
UniProt: A0A0D5YMB7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKISENWLRT WVNPAIDSDT LSDQLTMLGL EVDELAPVAK PFTGVVVGEV LTVEQHPDAD RLRVTTVNIG SGEPLQIVCG APNVRAGMKA PVATIGAILP GDFKIKKGKL RGVESQGMLC GASEIDLEDK IDGLLELPND APVGVNIREY LKLDDNVIDI SITPNRGDCF SIRGIAREVA VINQLQMNEP EIKSVDATIT DEKKVVINTD GAPRYLGRVI KNVNVKASTP EWMEQALARS GIRTHSILVD VTNYVLMELG QPMHAFDLAK IEGTVHVRQA QPQEKLQLLN DQEVELQDDV MVIADDQKAL AIAGIMGGLA SSVTDDTTDI FLESAFFAPL AIAGRARRFG LHTDSSQRYE RGVDFELPLI AMNRASQLIQ ELAGGEFGPI TVVEKSDLLP KREAIELKQA QVDQLLGYKV AAEFITDALT RLGCEVTVKA DGEWSVVPPS HRYDMAIYQD LIEEVARIDG YDNIQISLPS MDVQLAKYQD RFEIAQLRQT VVTLGYQEAI SFSFADAKLE KQLNPQVSPL MLANPISSDL AAMRSTLLSS LIPCVQYNLN RQQSRVRFFE LGLRFDYQNA NSIQDLKQIP TLALVAVGSR EPESWHAKPQ PMDFFDFKGE VEEILAAGRV KVEYVRSERP WLHPGQSAEI LVDGQSIGYL GRLHPSLENE LDLSTTWVAE LDQAAVLQSY VSNFTELSRF PSVRRDIALL ISDNINVRDI QQLIEKTGGE LLDSTWLFDV YTGQGVEEGK RSLAFALLWQ HPSRTLEDAE IKSGMDNIIQ VLENTYQATL RAS
NT Sequence
ATGAAGATTA GCGAAAATTG GTTACGCACA TGGGTTAACC CAGCAATTGA TAGCGATACA TTATCTGATC AGTTGACTAT GCTTGGTCTT GAAGTAGATG AGCTGGCACC TGTTGCTAAG CCATTTACTG GCGTTGTAGT TGGTGAAGTT CTAACAGTTG AACAGCACCC TGATGCAGAC CGTCTGCGTG TAACAACAGT GAATATTGGT TCAGGTGAGC CTTTACAAAT TGTATGTGGT GCTCCTAACG TTCGTGCTGG AATGAAAGCA CCTGTGGCAA CTATTGGTGC AATATTACCG GGTGATTTTA AAATCAAAAA AGGTAAGCTT CGTGGTGTTG AGTCACAAGG CATGCTGTGC GGTGCCTCTG AAATTGACCT TGAAGATAAA ATCGATGGTT TACTTGAGTT ACCTAATGAT GCTCCAGTAG GGGTAAACAT TCGTGAATAC TTAAAACTTG ATGATAATGT CATCGATATT AGTATTACAC CAAACCGTGG TGACTGCTTT AGTATTCGTG GTATCGCACG TGAAGTTGCA GTGATTAACC AACTGCAAAT GAATGAGCCT GAAATCAAAT CAGTTGATGC GACAATTACT GATGAGAAAA AAGTAGTCAT CAACACGGAT GGTGCTCCAC GCTACTTAGG ACGTGTCATT AAAAATGTAA ATGTTAAAGC TTCGACCCCA GAGTGGATGG AACAAGCTTT AGCACGTTCA GGAATTCGTA CTCATAGTAT TTTAGTTGAT GTAACGAATT ACGTCCTCAT GGAATTGGGT CAGCCAATGC ATGCTTTCGA TTTAGCTAAA ATCGAAGGGA CTGTACATGT TCGCCAAGCT CAGCCACAAG AAAAGTTACA GCTTTTAAAT GATCAAGAAG TTGAACTACA AGATGATGTG ATGGTTATTG CAGATGACCA AAAAGCACTA GCAATCGCTG GTATCATGGG TGGTCTAGCA TCAAGCGTAA CTGATGATAC GACTGATATC TTCTTGGAAA GCGCATTTTT TGCTCCGCTT GCGATTGCTG GACGTGCTCG TCGCTTTGGT TTACATACAG ATTCTTCACA ACGTTATGAA CGCGGTGTAG ATTTTGAATT GCCGTTAATT GCGATGAACC GTGCTTCTCA GCTTATTCAA GAGTTGGCGG GTGGTGAGTT CGGTCCAATT ACTGTAGTTG AAAAATCTGA TTTACTTCCG AAACGTGAAG CGATTGAACT TAAGCAAGCT CAAGTAGACC AGCTGTTGGG TTACAAAGTA GCGGCAGAGT TCATTACAGA TGCATTAACT CGATTAGGTT GTGAAGTCAC TGTTAAAGCG GATGGTGAAT GGAGCGTAGT TCCACCATCA CATCGCTACG ATATGGCAAT TTATCAAGAT TTGATTGAAG AAGTTGCACG TATTGATGGT TATGACAATA TTCAGATCAG CTTACCAAGT ATGGATGTTC AACTTGCTAA GTACCAAGAT CGTTTTGAAA TCGCGCAGTT ACGTCAAACG GTAGTCACTT TAGGGTATCA AGAAGCGATT AGCTTTAGCT TTGCTGATGC AAAACTTGAA AAGCAATTGA ACCCGCAAGT AAGTCCGTTA ATGTTGGCAA ATCCAATCTC TAGTGATTTG GCAGCAATGC GCAGCACATT GCTTTCGAGT TTAATCCCTT GTGTACAATA TAATTTAAAC CGTCAGCAAA GCCGTGTACG TTTCTTTGAA CTAGGTTTGC GTTTTGATTA TCAAAATGCT AATTCTATTC AGGATCTGAA GCAAATTCCT ACATTGGCTT TAGTTGCCGT AGGTTCTCGT GAACCTGAAT CATGGCATGC TAAGCCACAG CCAATGGATT TCTTCGATTT TAAAGGTGAA GTAGAAGAGA TTTTAGCTGC TGGACGTGTC AAAGTTGAGT ATGTACGTTC AGAGCGTCCA TGGTTGCATC CAGGGCAATC TGCTGAAATC TTAGTTGATG GCCAATCAAT TGGTTACTTG GGCCGCTTGC ATCCATCTTT AGAAAACGAA CTAGATTTGA GCACAACTTG GGTTGCCGAG CTTGATCAGG CTGCTGTTTT GCAATCTTAT GTATCTAATT TTACAGAATT ATCACGTTTT CCTTCGGTTA GACGTGATAT TGCGCTTTTA ATCTCTGATA ATATAAATGT TAGAGATATT CAGCAGTTAA TCGAAAAAAC TGGTGGAGAG CTTTTAGACT CTACTTGGTT ATTCGATGTG TATACGGGGC AGGGTGTCGA AGAAGGTAAA CGCTCATTAG CGTTTGCATT ATTATGGCAA CATCCTTCAC GTACGCTTGA AGATGCTGAA ATTAAATCTG GTATGGACAA TATAATCCAA GTGTTGGAAA ACACTTATCA AGCGACATTG AGGGCCTCAT GA
Details for AcbaC.18890.a.B1.GE38942
HARVESTED ON: 3/4/2015
SEQUENCED ON: 3/4/2015
EXPECTED MW: 89kDa
OBSERVED MW: 89kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMK ISENWLRTWV NPAIDSDTLS DQLTMLGLEV DELAPVAKPF TGVVVGEVLT VEQHPDADRL RVTTVNIGSG EPLQIVCGAP NVRAGMKAPV ATIGAILPGD FKIKKGKLRG VESQGMLCGA SEIDLEDKID GLLELPNDAP VGVNIREYLK LDDNVIDISI TPNRGDCFSI RGIAREVAVI NQLQMNEPEI KSVDATITDE KKVVINTDGA PRYLGRVIKN VNVKASTPEW MEQALARSGI RTHSILVDVT NYVLMELGQP MHAFDLAKIE GTVHVRQAQP QEKLQLLNDQ EVELQDDVMV IADDQKALAI AGIMGGLASS VTDDTTDIFL ESAFFAPLAI AGRARRFGLH TDSSQRYERG VDFELPLIAM NRASQLIQEL AGGEFGPITV VEKSDLLPKR EAIELKQAQV DQLLGYKVAA EFITDALTRL GCEVTVKADG EWSVVPPSHR YDMAIYQDLI EEVARIDGYD NIQISLPSMD VQLAKYQDRF EIAQLRQTVV TLGYQEAISF SFADAKLEKQ LNPQVSPLML ANPISSDLAA MRSTLLSSLI PCVQYNLNRQ QSRVRFFELG LRFDYQNANS IQDLKQIPTL ALVAVGSREP ESWHAKPQPM DFFDFKGEVE EILAAGRVKV EYVRSERPWL HPGQSAEILV DGQSIGYLGR LHPSLENELD LSTTWVAELD QAAVLQSYVS NFTELSRFPS VRRDIALLIS DNINVRDIQQ LIEKTGGELL DSTWLFDVYT GQGVEEGKRS LAFALLWQHP SRTLEDAEIK SGMDNIIQVL ENTYQATLRA S
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaga ttagcgaaaa ttggttacgc acatgggtta acccagcaat tgatagcgat acattatctg atcagttgac tatgcttggt cttgaagtag atgagctggc acctgttgct aagccattta ctggcgttgt agttggtgaa gttctaacag ttgaacagca ccctgatgca gaccgtctgc gtgtaacaac agtgaatatt ggttcaggtg agcctttaca aattgtatgt ggtgctccta acgttcgtgc tggaatgaaa gcacctgtgg caactattgg tgcaatatta ccgggtgatt ttaaaatcaa aaaaggtaag cttcgtggtg ttgagtcaca aggcatgctg tgcggtgcct ctgaaattga ccttgaagat aaaatcgatg gtttacttga gttacctaat gatgctccag taggggtaaa cattcgtgaa tacttaaaac ttgatgataa tgtcatcgat attagtatta caccaaaccg tggtgactgc tttagtattc gtggtatcgc acgtgaagtt gcagtgatta accaactgca aatgaatgag cctgaaatca aatcagttga tgcgacaatt actgatgaga aaaaagtagt catcaacacg gatggtgctc cacgctactt aggacgtgtc attaaaaatg taaatgttaa agcttcgacc ccagagtgga tggaacaagc tttagcacgt tcaggaattc gtactcatag tattttagtt gatgtaacga attacgtcct catggaattg ggtcagccaa tgcatgcttt cgatttagct aaaatcgaag ggactgtaca tgttcgccaa gctcagccac aagaaaagtt acagctttta aatgatcaag aagttgaact acaagatgat gtgatggtta ttgcagatga ccaaaaagca ctagcaatcg ctggtatcat gggtggtcta gcatcaagcg taactgatga tacgactgat atcttcttgg aaagcgcatt ttttgctccg cttgcgattg ctggacgtgc tcgtcgcttt ggtttacata cagattcttc acaacgttat gaacgcggtg tagattttga attgccgtta attgcgatga accgtgcttc tcagcttatt caagagttgg cgggtggtga gttcggtcca attactgtag ttgaaaaatc tgatttactt ccgaaacgtg aagcgattga acttaagcaa gctcaagtag accagctgtt gggttacaaa gtagcggcag agttcattac agatgcatta actcgattag gttgtgaagt cactgttaaa gcggatggtg aatggagcgt agttccacca tcacatcgct acgatatggc aatttatcaa gatttgattg aagaagttgc acgtattgat ggttatgaca atattcagat cagcttacca agtatggatg ttcaacttgc taagtaccaa gatcgttttg aaatcgcgca gttacgtcaa acggtagtca ctttagggta tcaagaagcg attagcttta gctttgctga tgcaaaactt gaaaagcaat tgaacccgca agtaagtccg ttaatgttgg caaatccaat ctctagtgat ttggcagcaa tgcgcagcac attgctttcg agtttaatcc cttgtgtaca atataattta aaccgtcagc aaagccgtgt acgtttcttt gaactaggtt tgcgttttga ttatcaaaat gctaattcta ttcaggatct gaagcaaatt cctacattgg ctttagttgc cgtaggttct cgtgaacctg aatcatggca tgctaagcca cagccaatgg atttcttcga ttttaaaggt gaagtagaag agattttagc tgctggacgt gtcaaagttg agtatgtacg ttcagagcgt ccatggttgc atccagggca atctgctgaa atcttagttg atggccaatc aattggttac ttgggccgct tgcatccatc tttagaaaac gaactagatt tgagcacaac ttgggttgcc gagcttgatc aggctgctgt tttgcaatct tatgtatcta attttacaga attatcacgt tttccttcgg ttagacgtga tattgcgctt ttaatctctg ataatataaa tgttagagat attcagcagt taatcgaaaa aactggtgga gagcttttag actctacttg gttattcgat gtgtatacgg ggcagggtgt cgaagaaggt aaacgctcat tagcgtttgc attattatgg caacatcctt cacgtacgct tgaagatgct gaaattaaat ctggtatgga caatataatc caagtgttgg aaaacactta tcaagcgaca ttgagggcct catgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat