AcbaC.18886.a
lipid A export permease/ATP-binding protein MsbA
| CENTER ID: | AcbaC.18886.a |
| ORGANISM: | Acinetobacter baumannii AB5075-UW |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| AcbaC.18886.a.B2.GE38939 | ATP-binding cassette | 305 | 575 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| AcbaC.18886.a.B2.PW37739 | ATP-binding cassette | 305 | 575 |
Structures
5IDV
DEPOSITED: 2/24/2016
DETERMINATION: XRay
CLONE: AcbaC.18886.a.B2.GE38939
PROTEIN: AcbaC.18886.a.B2.PW37739
Publications by SSGCID
Towards a structome of Acinetobacter baumannii drug targets
Abendroth J, Barrett LK, Dranow DM, Edwards TE, Lorimer DD, Myler PJ, Phan I, Staker B, Subramanian S, Van Voorhis WC, Craig JC, Chun IKH, Barrett KF, Prentice RA, Tillery L, Shek P
Protein Sci. - 2020
volume 29, issue 3, pages 789-802
PMID: 31930600; PMCID: PMC7020997
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| GenBank: | AKA31987.1 |
| PATRIC ID: | fig|470.1314.peg.2851 |
| PGAT: | acinetobacter:ABUW_2259 |
| RefSeq: | WP_001070729.1 |
| UniProt: | A0A098SJ45 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
VNQDFKVYLR LISYLKPYWG VALLVLIGFG MNSATEVSVA KLIKFIIDAI QNASRADLDW FPLLIILLVF FRGLGLFMGG YYTAVISRSL VFSIRQEVYA KLLRLPAQYY LDNSSGHITA KIMYNVEQLT AASSESLKTI VRDGMITLGL LGYLFYTNWR LTICIMVFLP VIGILVRKAS KRMRKLSMQV QDTMGDVNHV VQESINGNAV VKSFAGEESE QERFYKSSEE NLKRGLKMVI VQNLNSPVVQ VVMACAMALI VWLALRPQIL GNTTAGEFVA YITAAGLLSK PVKNLTDVNE KLQRGLAAAH SVFELLDLPE EQNSGELKPQ LQGAIRFDHV VLNYADGTQA IKDFSLDIRP GETVALVGRS GAGKTSLVNM LVRFQEVSSG QIYLDDLPIR DIELSSLRTQ IAMVNQQVVL FNRTVRENIA YGQLHNASDE DVIAAAKAAY AHDFIMNLPN GYDTVLGAQG LNLSGGQRQR IAIARAILKN APILILDEAT SALDNESEHF IQQAFDEAMQ DRTTIVIAHR LSTIENADRI VVMDRGQIVE QGTHQELLAK HGAYYQLHQR NFEDH
NT Sequence
GTGAATCAAG ATTTTAAGGT TTATTTGCGT CTCATATCCT ATTTAAAACC TTATTGGGGC GTGGCCTTAT TAGTTCTTAT CGGGTTCGGT ATGAACTCAG CAACGGAGGT TTCTGTTGCT AAGCTCATTA AATTTATTAT TGATGCAATT CAAAATGCGA GTAGAGCGGA TCTGGATTGG TTCCCTTTAT TAATCATTTT ATTGGTATTC TTCCGTGGCC TTGGCCTATT TATGGGGGGG TACTATACGG CTGTGATCTC GCGTAGTTTG GTTTTTAGTA TTCGCCAAGA AGTTTATGCA AAACTTCTAA GACTGCCGGC TCAATATTAT CTCGATAACT CTTCGGGTCA TATTACTGCC AAAATTATGT ATAACGTCGA GCAACTTACA GCTGCTTCAT CTGAATCATT AAAAACCATT GTTCGAGATG GCATGATTAC GTTGGGGTTG TTAGGTTATT TGTTCTATAC CAATTGGCGT TTAACAATTT GTATTATGGT GTTCTTACCT GTAATTGGGA TTTTGGTGCG TAAAGCTTCA AAAAGAATGC GCAAACTCTC GATGCAAGTT CAGGACACTA TGGGTGATGT TAACCATGTA GTGCAAGAAA GTATTAATGG TAATGCAGTT GTTAAAAGTT TCGCTGGTGA AGAGTCAGAA CAAGAGCGTT TCTATAAGTC ATCGGAAGAA AACTTAAAAC GTGGTTTAAA AATGGTGATT GTGCAAAACC TAAATAGTCC TGTTGTTCAA GTGGTAATGG CTTGTGCAAT GGCTCTAATT GTATGGTTAG CATTAAGACC ACAAATTTTA GGGAATACAA CTGCTGGTGA GTTTGTTGCC TATATTACGG CAGCAGGGTT ACTTTCAAAG CCTGTTAAAA ACTTGACAGA TGTGAATGAA AAGTTACAGC GGGGTTTAGC AGCTGCACAC TCAGTTTTTG AATTATTAGA TTTACCTGAA GAGCAAAATA GTGGTGAACT TAAACCGCAG TTGCAGGGTG CAATTCGTTT TGACCATGTT GTACTTAACT ATGCTGACGG TACGCAAGCG ATTAAAGACT TTTCTTTAGA TATTCGTCCA GGCGAAACTG TTGCATTGGT AGGGCGCTCT GGTGCTGGTA AAACTTCTTT AGTGAACATG TTGGTACGTT TCCAAGAAGT CTCAAGTGGT CAAATTTATT TAGATGATTT GCCAATACGT GATATTGAGC TTTCAAGTTT GCGTACACAA ATTGCAATGG TAAATCAGCA AGTTGTTTTG TTTAACCGAA CTGTACGTGA AAACATTGCT TATGGTCAGT TACATAATGC AAGTGATGAA GACGTAATAG CTGCAGCAAA GGCTGCTTAT GCCCACGACT TTATTATGAA CTTACCAAAT GGCTACGATA CTGTTTTGGG TGCTCAAGGT CTAAATTTAT CGGGTGGTCA ACGTCAACGT ATTGCAATTG CTCGTGCAAT TTTGAAAAAT GCCCCAATTC TCATTTTGGA TGAGGCGACA AGTGCGCTTG ATAATGAATC TGAACACTTT ATTCAACAAG CATTTGATGA GGCTATGCAA GATCGTACAA CGATTGTGAT TGCTCACCGT CTATCAACCA TTGAAAATGC GGATCGTATT GTTGTGATGG ATCGCGGACA AATTGTTGAA CAAGGTACCC ATCAAGAATT ACTTGCTAAG CACGGTGCTT ATTATCAATT ACATCAGCGT AATTTTGAGG ACCATTAA
Details for AcbaC.18886.a.B2.GE38939
| HARVESTED ON: | 3/4/2015 |
| SEQUENCED ON: | 3/4/2015 |
| EXPECTED MW: | 31kDa |
| OBSERVED MW: | 31kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG LAAAHSVFEL LDLPEEQNSG ELKPQLQGAI RFDHVVLNYA DGTQAIKDFS LDIRPGETVA LVGRSGAGKT SLVNMLVRFQ EVSSGQIYLD DLPIRDIELS SLRTQIAMVN QQVVLFNRTV RENIAYGQLH NASDEDVIAA AKAAYAHDFI MNLPNGYDTV LGAQGLNLSG GQRQRIAIAR AILKNAPILI LDEATSALDN ESEHFIQQAF DEAMQDRTTI VIAHRLSTIE NADRIVVMDR GQIVEQGTHQ ELLAKHGAYY QLHQRNFEDH
Validated NT Sequence
atcttactca cttaatggtc ctcaaaatta cgctgatgta attgataata agcaccgtgc ttagcaagta attcttgatg ggtaccttgt tcaacaattt gtccgcgatc catcacaaca atacgatccg cattttcaat ggttgataga cggtgagcaa tcacaatcgt tgtacgatct tgcatagcct catcaaatgc ttgttgaata aagtgttcag attcattatc aagcgcactt gtcgcctcat ccaaaatgag aattggggca tttttcaaaa ttgcacgagc aattgcaata cgttgacgtt gaccacccga taaatttaga ccttgagcac ccaaaacagt atcgtagcca tttggtaagt tcataataaa gtcgtgggca taagcagcct ttgctgcagc tattacgtct tcatcacttg cattatgtaa ctgaccataa gcaatgtttt cacgtacagt tcggttaaac aaaacaactt gctgatttac cattgcaatt tgtgtacgca aacttgaaag ctcaatatca cgtattggca aatcatctaa ataaatttga ccacttgaga cttcttggaa acgtaccaac atgttcacta aagaagtttt accagcacca gagcgcccta ccaatgcaac agtttcgcct ggacgaatat ctaaagaaaa gtctttaatc gcttgcgtac cgtcagcata gttaagtaca acatggtcaa aacgaattgc accctgcaac tgcggtttaa gttcaccact attttgctct tcaggtaaat ctaataattc aaaaactgag tgtgcagctg ctaaacccat atggtggtgg tggtggtgag ccat
Expected Protein Sequence
MAHHHHHHGL AAAHSVFELL DLPEEQNSGE LKPQLQGAIR FDHVVLNYAD GTQAIKDFSL DIRPGETVAL VGRSGAGKTS LVNMLVRFQE VSSGQIYLDD LPIRDIELSS LRTQIAMVNQ QVVLFNRTVR ENIAYGQLHN ASDEDVIAAA KAAYAHDFIM NLPNGYDTVL GAQGLNLSGG QRQRIAIARA ILKNAPILIL DEATSALDNE SEHFIQQAFD EAMQDRTTIV IAHRLSTIEN ADRIVVMDRG QIVEQGTHQE LLAKHGAYYQ LHQRNFEDH
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catggtttag cagctgcaca ctcagttttt gaattattag atttacctga agagcaaaat agtggtgaac ttaaaccgca gttgcagggt gcaattcgtt ttgaccatgt tgtacttaac tatgctgacg gtacgcaagc gattaaagac ttttctttag atattcgtcc aggcgaaact gttgcattgg tagggcgctc tggtgctggt aaaacttctt tagtgaacat gttggtacgt ttccaagaag tctcaagtgg tcaaatttat ttagatgatt tgccaatacg tgatattgag ctttcaagtt tgcgtacaca aattgcaatg gtaaatcagc aagttgtttt gtttaaccga actgtacgtg aaaacattgc ttatggtcag ttacataatg caagtgatga agacgtaata gctgcagcaa aggctgctta tgcccacgac tttattatga acttaccaaa tggctacgat actgttttgg gtgctcaagg tctaaattta tcgggtggtc aacgtcaacg tattgcaatt gctcgtgcaa ttttgaaaaa tgccccaatt ctcattttgg atgaggcgac aagtgcgctt gataatgaat ctgaacactt tattcaacaa gcatttgatg aggctatgca agatcgtaca acgattgtga ttgctcaccg tctatcaacc attgaaaatg cggatcgtat tgttgtgatg gatcgcggac aaattgttga acaaggtacc catcaagaat tacttgctaa gcacggtgct tattatcaat tacatcagcg taattttgag gaccattgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for AcbaC.18886.a.B2.PW37739
| PURIFICATION DATe: | 7/7/2015 |
| CONCENTRATION: | 26mg/ml |
| OBSERVED MW: | 32kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 21 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG LAAAHSVFEL LDLPEEQNSG ELKPQLQGAI RFDHVVLNYA DGTQAIKDFS LDIRPGETVA LVGRSGAGKT SLVNMLVRFQ EVSSGQIYLD DLPIRDIELS SLRTQIAMVN QQVVLFNRTV RENIAYGQLH NASDEDVIAA AKAAYAHDFI MNLPNGYDTV LGAQGLNLSG GQRQRIAIAR AILKNAPILI LDEATSALDN ESEHFIQQAF DEAMQDRTTI VIAHRLSTIE NADRIVVMDR GQIVEQGTHQ ELLAKHGAYY QLHQRNFEDH
Validated NT Sequence
atcttactca cttaatggtc ctcaaaatta cgctgatgta attgataata agcaccgtgc ttagcaagta attcttgatg ggtaccttgt tcaacaattt gtccgcgatc catcacaaca atacgatccg cattttcaat ggttgataga cggtgagcaa tcacaatcgt tgtacgatct tgcatagcct catcaaatgc ttgttgaata aagtgttcag attcattatc aagcgcactt gtcgcctcat ccaaaatgag aattggggca tttttcaaaa ttgcacgagc aattgcaata cgttgacgtt gaccacccga taaatttaga ccttgagcac ccaaaacagt atcgtagcca tttggtaagt tcataataaa gtcgtgggca taagcagcct ttgctgcagc tattacgtct tcatcacttg cattatgtaa ctgaccataa gcaatgtttt cacgtacagt tcggttaaac aaaacaactt gctgatttac cattgcaatt tgtgtacgca aacttgaaag ctcaatatca cgtattggca aatcatctaa ataaatttga ccacttgaga cttcttggaa acgtaccaac atgttcacta aagaagtttt accagcacca gagcgcccta ccaatgcaac agtttcgcct ggacgaatat ctaaagaaaa gtctttaatc gcttgcgtac cgtcagcata gttaagtaca acatggtcaa aacgaattgc accctgcaac tgcggtttaa gttcaccact attttgctct tcaggtaaat ctaataattc aaaaactgag tgtgcagctg ctaaacccat atggtggtgg tggtggtgag ccat
Expressed Protein Sequence
MAHHHHHHGL AAAHSVFELL DLPEEQNSGE LKPQLQGAIR FDHVVLNYAD GTQAIKDFSL DIRPGETVAL VGRSGAGKTS LVNMLVRFQE VSSGQIYLDD LPIRDIELSS LRTQIAMVNQ QVVLFNRTVR ENIAYGQLHN ASDEDVIAAA KAAYAHDFIM NLPNGYDTVL GAQGLNLSGG QRQRIAIARA ILKNAPILIL DEATSALDNE SEHFIQQAFD EAMQDRTTIV IAHRLSTIEN ADRIVVMDRG QIVEQGTHQE LLAKHGAYYQ LHQRNFEDH
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGGTTTAG CAGCTGCACA CTCAGTTTTT GAATTATTAG ATTTACCTGA AGAGCAAAAT AGTGGTGAAC TTAAACCGCA GTTGCAGGGT GCAATTCGTT TTGACCATGT TGTACTTAAC TATGCTGACG GTACGCAAGC GATTAAAGAC TTTTCTTTAG ATATTCGTCC AGGCGAAACT GTTGCATTGG TAGGGCGCTC TGGTGCTGGT AAAACTTCTT TAGTGAACAT GTTGGTACGT TTCCAAGAAG TCTCAAGTGG TCAAATTTAT TTAGATGATT TGCCAATACG TGATATTGAG CTTTCAAGTT TGCGTACACA AATTGCAATG GTAAATCAGC AAGTTGTTTT GTTTAACCGA ACTGTACGTG AAAACATTGC TTATGGTCAG TTACATAATG CAAGTGATGA AGACGTAATA GCTGCAGCAA AGGCTGCTTA TGCCCACGAC TTTATTATGA ACTTACCAAA TGGCTACGAT ACTGTTTTGG GTGCTCAAGG TCTAAATTTA TCGGGTGGTC AACGTCAACG TATTGCAATT GCTCGTGCAA TTTTGAAAAA TGCCCCAATT CTCATTTTGG ATGAGGCGAC AAGTGCGCTT GATAATGAAT CTGAACACTT TATTCAACAA GCATTTGATG AGGCTATGCA AGATCGTACA ACGATTGTGA TTGCTCACCG TCTATCAACC ATTGAAAATG CGGATCGTAT TGTTGTGATG GATCGCGGAC AAATTGTTGA ACAAGGTACC CATCAAGAAT TACTTGCTAA GCACGGTGCT TATTATCAAT TACATCAGCG TAATTTTGAG GACCATTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T