AcbaC.18885.a
lipoprotein-releasing system transmembrane protein LolE
| CENTER ID: | AcbaC.18885.a |
| ORGANISM: | Acinetobacter baumannii AB5075-UW |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
Clicking the button will add the material to a virtual cart.
You may order multiple materials at a time at no cost to you, as this contract is funded
by NIAID. When you are ready to place your order, click the "Place Order" link which will
appear in the top right corner of the page after you place your first item in your cart.
Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| AcbaC.18885.a.B2.GE38938 | MacB-like periplasmic core domain | 63 | 284 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
5UDF
DEPOSITED: 12/26/2016
DETERMINATION: XRay
CLONE: AcbaC.18885.a.B2.GE38938
PROTEIN: AcbaC.18885.a.B2.PS02462
Publications by SSGCID
Towards a structome of Acinetobacter baumannii drug targets
Abendroth J, Barrett LK, Dranow DM, Edwards TE, Lorimer DD, Myler PJ, Phan I, Staker B, Subramanian S, Van Voorhis WC, Craig JC, Chun IKH, Barrett KF, Prentice RA, Tillery L, Shek P
Protein Sci. - 2020
volume 29, issue 3, pages 789-802
PMID: 31930600; PMCID: PMC7020997
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| GenBank: | AKA30728.1 |
| PATRIC ID: | fig|470.1345.peg.939 |
| PGAT: | acinetobacter:ABUW_0974 |
| UniProt: | A0A0D5YFP2 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNTSLFPSMT LDLEGMFKPI SLYIGLRYTR ARRSNHFISF IALVSMVGLT LGVAVLITVL SVMNGFDREL KNRVLGMVPQ ATVSSTQILT DWPELVKRVE NHPHVTGVAP FTQLQGMLTA QGQVAGIMVT GIDPKYEKNV SIIQNHIVAG SLDSLKKGEF GIVLGKDMAD SLGLRLNDSV TLVLPEATPS PAGVVPRFKR FKVVGIFSVG AEVDSMVGYI ALYDASTLLR LPDGAQGVRL KLDDIFAAPQ VADDIVKNLP SNFYATNWTY THGNLFNAIQ MEKTLVGLLL VLIIVVAAFN IVSSLVMVVT DKKSDIAILR TLGASPSMIT KIFMVQGTVI GVIGTVAGTV LGVILALTIS DIISWFNNVL GLNLFDAYFV HYLPSYLRWQ DVTIIVIVSL LLSFLATIYP ALRAAKVQPA EALRYE
NT Sequence
ATGAACACTT CTCTTTTTCC ATCAATGACC TTGGATTTAG AAGGTATGTT TAAACCAATC TCACTGTATA TAGGGTTGAG ATATACTCGC GCTCGACGCA GTAATCATTT TATTTCTTTT ATTGCATTGG TTTCGATGGT CGGTTTGACA CTTGGTGTCG CTGTACTAAT TACGGTACTT TCTGTAATGA ACGGTTTCGA CCGCGAATTA AAAAATCGTG TTTTGGGAAT GGTACCGCAA GCGACAGTTT CATCGACGCA AATCCTTACG GATTGGCCTG AACTGGTTAA ACGAGTTGAA AATCATCCCC ACGTGACAGG AGTTGCACCT TTTACCCAAT TGCAAGGTAT GTTAACTGCT CAAGGGCAAG TGGCAGGTAT TATGGTGACG GGTATTGATC CTAAATATGA AAAGAATGTC TCTATCATTC AAAACCATAT TGTTGCCGGT AGTCTCGACT CACTTAAAAA AGGTGAATTC GGTATCGTAC TTGGTAAAGA CATGGCCGAT TCGCTTGGCT TACGTTTAAA CGATAGCGTG ACATTGGTTT TACCTGAAGC GACTCCATCT CCAGCTGGTG TTGTACCTCG TTTTAAACGC TTTAAAGTGG TAGGTATTTT CAGTGTAGGT GCTGAAGTTG ACTCGATGGT TGGATACATT GCTTTATATG ATGCTTCAAC CTTATTGCGT TTACCTGATG GCGCTCAAGG CGTTCGTCTA AAATTAGATG ATATTTTTGC CGCTCCTCAA GTTGCCGATG ACATTGTGAA AAATTTACCA AGCAATTTCT ACGCAACCAA CTGGACTTAT ACACATGGCA ACTTATTTAA TGCCATCCAA ATGGAAAAAA CATTAGTTGG TTTATTGCTT GTACTGATTA TTGTTGTTGC TGCATTTAAC ATTGTTTCGT CTTTAGTTAT GGTCGTGACT GACAAAAAAT CAGACATTGC AATTTTACGT ACTTTAGGTG CATCGCCATC TATGATTACT AAAATCTTTA TGGTGCAAGG TACGGTTATT GGTGTGATCG GTACAGTTGC GGGTACGGTT TTAGGGGTAA TTCTGGCACT TACGATCAGC GATATTATTT CTTGGTTTAA TAATGTTCTC GGACTTAATC TGTTCGATGC CTATTTTGTA CATTACTTAC CTTCTTACTT AAGATGGCAA GACGTGACAA TTATTGTGAT TGTTTCTTTA CTCTTAAGTT TCTTGGCAAC TATTTATCCG GCGCTTCGTG CAGCCAAAGT TCAACCTGCC GAGGCATTGC GTTATGAGTA A
Details for AcbaC.18885.a.B2.GE38938
| HARVESTED ON: | 3/4/2015 |
| SEQUENCED ON: | 3/4/2015 |
| EXPECTED MW: | 25kDa |
| OBSERVED MW: | 25kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMN GFDRELKNRV LGMVPQATVS STQILTDWPE LVKRVENHPH VTGVAPFTQL QGMLTAQGQV AGIMVTGIDP KYEKNVSIIQ NHIVAGSLDS LKKGEFGIVL GKDMADSLGL RLNDSVTLVL PEATPSPAGV VPRFKRFKVV GIFSVGAEVD SMVGYIALYD ASTLLRLPDG AQGVRLKLDD IFAAPQVADD IVKNLPSNFY ATNWTYTHGN LFNAIQMEKT
Validated NT Sequence
atggctcacc accaccacca ccatatgaac ggtttcgacc gcgaattaaa aaatcgtgtt ttgggaatgg taccgcaagc gacagtttca tcgacgcaaa tccttacgga ttggcctgaa ctggttaaac gagttgaaaa tcatccccac gtgacaggag ttgcaccttt tacccaattg caaggtatgt taactgctca agggcaagtg gcaggtatta tggtgacggg tattgatcct aaatatgaaa agaatgtctc tatcattcaa aaccatattg ttgccggtag tctcgactca cttaaaaaag gtgaattcgg tatcgtactt ggtaaagaca tggccgattc gcttggctta cgtttaaacg atagcgtgac attggtttta cctgaagcga ctccatctcc agctggtgtt gtacctcgtt ttaaacgctt taaagtggta ggtattttca gtgtaggtgc tgaagttgac tcgatggttg gatacattgc tttatatgat gcttcaacct tattgcgttt acctgatggc gctcaaggcg ttcgtctaaa attagatgat atttttgccg ctcctcaagt tgccgatgac attgtgaaaa atttaccaag caatttctac gcaaccaact ggacttatac acatggcaac ttatttaatg ccatccaaat ggaaaaaaca taagtgagta agat
Expected Protein Sequence
MAHHHHHHMN GFDRELKNRV LGMVPQATVS STQILTDWPE LVKRVENHPH VTGVAPFTQL QGMLTAQGQV AGIMVTGIDP KYEKNVSIIQ NHIVAGSLDS LKKGEFGIVL GKDMADSLGL RLNDSVTLVL PEATPSPAGV VPRFKRFKVV GIFSVGAEVD SMVGYIALYD ASTLLRLPDG AQGVRLKLDD IFAAPQVADD IVKNLPSNFY ATNWTYTHGN LFNAIQMEKT
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaacg gtttcgaccg cgaattaaaa aatcgtgttt tgggaatggt accgcaagcg acagtttcat cgacgcaaat ccttacggat tggcctgaac tggttaaacg agttgaaaat catccccacg tgacaggagt tgcacctttt acccaattgc aaggtatgtt aactgctcaa gggcaagtgg caggtattat ggtgacgggt attgatccta aatatgaaaa gaatgtctct atcattcaaa accatattgt tgccggtagt ctcgactcac ttaaaaaagg tgaattcggt atcgtacttg gtaaagacat ggccgattcg cttggcttac gtttaaacga tagcgtgaca ttggttttac ctgaagcgac tccatctcca gctggtgttg tacctcgttt taaacgcttt aaagtggtag gtattttcag tgtaggtgct gaagttgact cgatggttgg atacattgct ttatatgatg cttcaacctt attgcgttta cctgatggcg ctcaaggcgt tcgtctaaaa ttagatgata tttttgccgc tcctcaagtt gccgatgaca ttgtgaaaaa tttaccaagc aatttctacg caaccaactg gacttataca catggcaact tatttaatgc catccaaatg gaaaaaacat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat