AcbaC.18873.a
diaminopimelate epimerase
| CENTER ID: | AcbaC.18873.a |
| ORGANISM: | Acinetobacter baumannii AB5075-UW |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| AcbaC.18873.a.B1.GE38937 | full length | 1 | 281 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| AcbaC.18873.a.B1.PW37762 | full length | 1 | 281 | ||
| AcbaC.18873.a.B1.PW37763 | full length | 1 | 281 |
Structures
5HA4
DEPOSITED: 12/30/2015
DETERMINATION: XRay
CLONE: AcbaC.18873.a.B1.GE38937
PROTEIN: AcbaC.18873.a.B1.PW37762
Publications by SSGCID
Towards a structome of Acinetobacter baumannii drug targets
Abendroth J, Barrett LK, Dranow DM, Edwards TE, Lorimer DD, Myler PJ, Phan I, Staker B, Subramanian S, Van Voorhis WC, Craig JC, Chun IKH, Barrett KF, Prentice RA, Tillery L, Shek P
Protein Sci. - 2020
volume 29, issue 3, pages 789-802
PMID: 31930600; PMCID: PMC7020997
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| GenBank: | AKA30708.1 |
| PATRIC ID: | fig|470.1345.peg.918 |
| PGAT: | acinetobacter:ABUW_0954 |
| RefSeq: | WP_000923484.1 |
| UniProt: | A0A0D8GNZ8 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLLEFTKMHG LGNDFMVVDL ISQRAYLDTA TIQRLADRHF GVGFDQLLIV EPPDVPEADF KYRIFNADGS EVEQCGNGVR CFARFVHERH LTNKTNITVQ TKAGIVKPEL GQNGWVRVNM GYPKFLPNEI PFVAEEPEAL YTLELANDQN ISIDVVNMGN PHAVTIVPDV LTADVAGIGP QVESHKRFPE RVNAGFMQVI DDKHVRLRVF ERGVGETLAC GTGACAAAVS GMRRGLLANS VEVELAGGKL QIEWQEGDVV WMTGPTTHVY DGRLDLRYFQ G
NT Sequence
ATGTTATTAG AATTTACCAA AATGCATGGT TTAGGCAATG ATTTCATGGT GGTTGATCTA ATTAGCCAAA GAGCTTATTT AGATACTGCA ACAATTCAAC GTTTGGCAGA CCGACACTTT GGTGTGGGGT TTGATCAGCT TTTAATTGTT GAACCACCAG ATGTACCCGA AGCTGATTTT AAATACCGTA TTTTCAATGC AGATGGTTCT GAGGTTGAGC AATGTGGTAA TGGTGTGCGT TGTTTTGCCC GTTTTGTCCA CGAACGTCAT TTAACCAATA AAACCAACAT TACCGTACAA ACCAAAGCGG GTATTGTAAA GCCAGAGCTT GGGCAAAATG GTTGGGTGCG CGTAAATATG GGCTACCCAA AGTTTCTACC AAATGAAATT CCATTTGTTG CCGAAGAGCC AGAAGCTTTA TATACCCTTG AGTTGGCAAA TGACCAAAAC ATTAGTATTG ATGTGGTCAA CATGGGTAAC CCACACGCGG TCACTATAGT TCCAGATGTA CTAACTGCTG ATGTAGCTGG TATTGGTCCG CAAGTTGAAT CACATAAACG TTTCCCTGAG CGCGTTAATG CTGGTTTCAT GCAAGTGATT GATGACAAGC ATGTTCGCTT ACGTGTATTT GAACGTGGTG TGGGTGAAAC ACTTGCGTGT GGTACAGGAG CTTGTGCTGC TGCGGTAAGT GGCATGCGCC GTGGTCTACT TGCGAACTCT GTAGAAGTTG AACTTGCTGG TGGTAAGCTA CAAATTGAAT GGCAAGAAGG CGATGTGGTC TGGATGACTG GTCCAACAAC TCATGTATAT GATGGACGTT TGGATTTACG TTATTTCCAA GGTTAA
Details for AcbaC.18873.a.B1.GE38937
| HARVESTED ON: | 3/4/2015 |
| SEQUENCED ON: | 3/4/2015 |
| EXPECTED MW: | 32kDa |
| OBSERVED MW: | 32kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHML LEFTKMHGLG NDFMVVDLIS QRAYLDTATI QRLADRHFGV GFDQLLIVEP PDVPEADFKY RIFNADGSEV EQCGNGVRCF ARFVHERHLT NKTNITVQTK AGIVKPELGQ NGWVRVNMGY PKFLPNEIPF VAEEPEALYT LELANDQNIS IDVVNMGNPH AVTIVPDVLT ADVAGIGPQV ESHKRFPERV NAGFMQVIDD KHVRLRVFER GVGETLACGT GACAAAVSGM RRGLLANSVE VELAGGKLQI EWQEGDVVWM TGPTTHVYDG RLDLRYFQG
Validated NT Sequence
ttactcactt aaccttggaa ataacgtaaa tccaaacgtc catcatatac atgagttgtt ggaccagtca tccagaccac atcgccttct tgccattcaa tttgtagctt accaccagca agttcaactt ctacagagtt cgcaagtaga ccacggcgca tgccacttac cgcagcagca caagctcctg taccacacgc aagtgtttca cccacaccac gttcaaatac acgtaagcga acatgcttgt catcaatcac ttgcatgaaa ccagcattaa cgcgctcagg gaaacgttta tgtgattcaa cttgcggacc aataccagct acatcagcag ttagtacatc tggaactata gtgaccgcgt gtgggttacc catgttgacc acatcaatac taatgttttg gtcatttgcc aactcaaggg tatataaagc ttctggctct tcggcaacaa atggaatttc atttggtaga aactttgggt agcccatatt tacgcgcacc caaccatttt gcccaagctc tggctttaca atacccgctt tggtttgtac ggtaatgttg gttttattgg ttaaatgacg ttcgtggaca aaacgggcaa aacaacgcac accattacca cattgctcaa cctcagaacc atctgcattg aaaatacggt atttaaaatc agcttcgggt acatctggtg gttcaacaat taaaagctga tcaaacccca caccaaagtg tcggtctgcc aaacgttgaa ttgttgcagt atctaaataa gctctttggc taattagatc aaccaccatg aaatcattgc ctaaaccatg cattttggta aattctaata acatatggtg gtggtggtgg tgagccat
Expected Protein Sequence
MAHHHHHHML LEFTKMHGLG NDFMVVDLIS QRAYLDTATI QRLADRHFGV GFDQLLIVEP PDVPEADFKY RIFNADGSEV EQCGNGVRCF ARFVHERHLT NKTNITVQTK AGIVKPELGQ NGWVRVNMGY PKFLPNEIPF VAEEPEALYT LELANDQNIS IDVVNMGNPH AVTIVPDVLT ADVAGIGPQV ESHKRFPERV NAGFMQVIDD KHVRLRVFER GVGETLACGT GACAAAVSGM RRGLLANSVE VELAGGKLQI EWQEGDVVWM TGPTTHVYDG RLDLRYFQG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttat tagaatttac caaaatgcat ggtttaggca atgatttcat ggtggttgat ctaattagcc aaagagctta tttagatact gcaacaattc aacgtttggc agaccgacac tttggtgtgg ggtttgatca gcttttaatt gttgaaccac cagatgtacc cgaagctgat tttaaatacc gtattttcaa tgcagatggt tctgaggttg agcaatgtgg taatggtgtg cgttgttttg cccgttttgt ccacgaacgt catttaacca ataaaaccaa cattaccgta caaaccaaag cgggtattgt aaagccagag cttgggcaaa atggttgggt gcgcgtaaat atgggctacc caaagtttct accaaatgaa attccatttg ttgccgaaga gccagaagct ttatataccc ttgagttggc aaatgaccaa aacattagta ttgatgtggt caacatgggt aacccacacg cggtcactat agttccagat gtactaactg ctgatgtagc tggtattggt ccgcaagttg aatcacataa acgtttccct gagcgcgtta atgctggttt catgcaagtg attgatgaca agcatgttcg cttacgtgta tttgaacgtg gtgtgggtga aacacttgcg tgtggtacag gagcttgtgc tgctgcggta agtggcatgc gccgtggtct acttgcgaac tctgtagaag ttgaacttgc tggtggtaag ctacaaattg aatggcaaga aggcgatgtg gtctggatga ctggtccaac aactcatgta tatgatggac gtttggattt acgttatttc caaggttgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for AcbaC.18873.a.B1.PW37762
| PURIFICATION DATe: | 8/4/2015 |
| CONCENTRATION: | 28mg/ml |
| OBSERVED MW: | 36kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 3 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHML LEFTKMHGLG NDFMVVDLIS QRAYLDTATI QRLADRHFGV GFDQLLIVEP PDVPEADFKY RIFNADGSEV EQCGNGVRCF ARFVHERHLT NKTNITVQTK AGIVKPELGQ NGWVRVNMGY PKFLPNEIPF VAEEPEALYT LELANDQNIS IDVVNMGNPH AVTIVPDVLT ADVAGIGPQV ESHKRFPERV NAGFMQVIDD KHVRLRVFER GVGETLACGT GACAAAVSGM RRGLLANSVE VELAGGKLQI EWQEGDVVWM TGPTTHVYDG RLDLRYFQG
Validated NT Sequence
ttactcactt aaccttggaa ataacgtaaa tccaaacgtc catcatatac atgagttgtt ggaccagtca tccagaccac atcgccttct tgccattcaa tttgtagctt accaccagca agttcaactt ctacagagtt cgcaagtaga ccacggcgca tgccacttac cgcagcagca caagctcctg taccacacgc aagtgtttca cccacaccac gttcaaatac acgtaagcga acatgcttgt catcaatcac ttgcatgaaa ccagcattaa cgcgctcagg gaaacgttta tgtgattcaa cttgcggacc aataccagct acatcagcag ttagtacatc tggaactata gtgaccgcgt gtgggttacc catgttgacc acatcaatac taatgttttg gtcatttgcc aactcaaggg tatataaagc ttctggctct tcggcaacaa atggaatttc atttggtaga aactttgggt agcccatatt tacgcgcacc caaccatttt gcccaagctc tggctttaca atacccgctt tggtttgtac ggtaatgttg gttttattgg ttaaatgacg ttcgtggaca aaacgggcaa aacaacgcac accattacca cattgctcaa cctcagaacc atctgcattg aaaatacggt atttaaaatc agcttcgggt acatctggtg gttcaacaat taaaagctga tcaaacccca caccaaagtg tcggtctgcc aaacgttgaa ttgttgcagt atctaaataa gctctttggc taattagatc aaccaccatg aaatcattgc ctaaaccatg cattttggta aattctaata acatatggtg gtggtggtgg tgagccat
Expressed Protein Sequence
MAHHHHHHML LEFTKMHGLG NDFMVVDLIS QRAYLDTATI QRLADRHFGV GFDQLLIVEP PDVPEADFKY RIFNADGSEV EQCGNGVRCF ARFVHERHLT NKTNITVQTK AGIVKPELGQ NGWVRVNMGY PKFLPNEIPF VAEEPEALYT LELANDQNIS IDVVNMGNPH AVTIVPDVLT ADVAGIGPQV ESHKRFPERV NAGFMQVIDD KHVRLRVFER GVGETLACGT GACAAAVSGM RRGLLANSVE VELAGGKLQI EWQEGDVVWM TGPTTHVYDG RLDLRYFQG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTTAT TAGAATTTAC CAAAATGCAT GGTTTAGGCA ATGATTTCAT GGTGGTTGAT CTAATTAGCC AAAGAGCTTA TTTAGATACT GCAACAATTC AACGTTTGGC AGACCGACAC TTTGGTGTGG GGTTTGATCA GCTTTTAATT GTTGAACCAC CAGATGTACC CGAAGCTGAT TTTAAATACC GTATTTTCAA TGCAGATGGT TCTGAGGTTG AGCAATGTGG TAATGGTGTG CGTTGTTTTG CCCGTTTTGT CCACGAACGT CATTTAACCA ATAAAACCAA CATTACCGTA CAAACCAAAG CGGGTATTGT AAAGCCAGAG CTTGGGCAAA ATGGTTGGGT GCGCGTAAAT ATGGGCTACC CAAAGTTTCT ACCAAATGAA ATTCCATTTG TTGCCGAAGA GCCAGAAGCT TTATATACCC TTGAGTTGGC AAATGACCAA AACATTAGTA TTGATGTGGT CAACATGGGT AACCCACACG CGGTCACTAT AGTTCCAGAT GTACTAACTG CTGATGTAGC TGGTATTGGT CCGCAAGTTG AATCACATAA ACGTTTCCCT GAGCGCGTTA ATGCTGGTTT CATGCAAGTG ATTGATGACA AGCATGTTCG CTTACGTGTA TTTGAACGTG GTGTGGGTGA AACACTTGCG TGTGGTACAG GAGCTTGTGC TGCTGCGGTA AGTGGCATGC GCCGTGGTCT ACTTGCGAAC TCTGTAGAAG TTGAACTTGC TGGTGGTAAG CTACAAATTG AATGGCAAGA AGGCGATGTG GTCTGGATGA CTGGTCCAAC AACTCATGTA TATGATGGAC GTTTGGATTT ACGTTATTTC CAAGGTTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T
Details for AcbaC.18873.a.B1.PW37763
| PURIFICATION DATe: | 8/4/2015 |
| CONCENTRATION: | 28mg/ml |
| OBSERVED MW: | 36kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 6 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHML LEFTKMHGLG NDFMVVDLIS QRAYLDTATI QRLADRHFGV GFDQLLIVEP PDVPEADFKY RIFNADGSEV EQCGNGVRCF ARFVHERHLT NKTNITVQTK AGIVKPELGQ NGWVRVNMGY PKFLPNEIPF VAEEPEALYT LELANDQNIS IDVVNMGNPH AVTIVPDVLT ADVAGIGPQV ESHKRFPERV NAGFMQVIDD KHVRLRVFER GVGETLACGT GACAAAVSGM RRGLLANSVE VELAGGKLQI EWQEGDVVWM TGPTTHVYDG RLDLRYFQG
Validated NT Sequence
ttactcactt aaccttggaa ataacgtaaa tccaaacgtc catcatatac atgagttgtt ggaccagtca tccagaccac atcgccttct tgccattcaa tttgtagctt accaccagca agttcaactt ctacagagtt cgcaagtaga ccacggcgca tgccacttac cgcagcagca caagctcctg taccacacgc aagtgtttca cccacaccac gttcaaatac acgtaagcga acatgcttgt catcaatcac ttgcatgaaa ccagcattaa cgcgctcagg gaaacgttta tgtgattcaa cttgcggacc aataccagct acatcagcag ttagtacatc tggaactata gtgaccgcgt gtgggttacc catgttgacc acatcaatac taatgttttg gtcatttgcc aactcaaggg tatataaagc ttctggctct tcggcaacaa atggaatttc atttggtaga aactttgggt agcccatatt tacgcgcacc caaccatttt gcccaagctc tggctttaca atacccgctt tggtttgtac ggtaatgttg gttttattgg ttaaatgacg ttcgtggaca aaacgggcaa aacaacgcac accattacca cattgctcaa cctcagaacc atctgcattg aaaatacggt atttaaaatc agcttcgggt acatctggtg gttcaacaat taaaagctga tcaaacccca caccaaagtg tcggtctgcc aaacgttgaa ttgttgcagt atctaaataa gctctttggc taattagatc aaccaccatg aaatcattgc ctaaaccatg cattttggta aattctaata acatatggtg gtggtggtgg tgagccat
Expressed Protein Sequence
MAHHHHHHML LEFTKMHGLG NDFMVVDLIS QRAYLDTATI QRLADRHFGV GFDQLLIVEP PDVPEADFKY RIFNADGSEV EQCGNGVRCF ARFVHERHLT NKTNITVQTK AGIVKPELGQ NGWVRVNMGY PKFLPNEIPF VAEEPEALYT LELANDQNIS IDVVNMGNPH AVTIVPDVLT ADVAGIGPQV ESHKRFPERV NAGFMQVIDD KHVRLRVFER GVGETLACGT GACAAAVSGM RRGLLANSVE VELAGGKLQI EWQEGDVVWM TGPTTHVYDG RLDLRYFQG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTTAT TAGAATTTAC CAAAATGCAT GGTTTAGGCA ATGATTTCAT GGTGGTTGAT CTAATTAGCC AAAGAGCTTA TTTAGATACT GCAACAATTC AACGTTTGGC AGACCGACAC TTTGGTGTGG GGTTTGATCA GCTTTTAATT GTTGAACCAC CAGATGTACC CGAAGCTGAT TTTAAATACC GTATTTTCAA TGCAGATGGT TCTGAGGTTG AGCAATGTGG TAATGGTGTG CGTTGTTTTG CCCGTTTTGT CCACGAACGT CATTTAACCA ATAAAACCAA CATTACCGTA CAAACCAAAG CGGGTATTGT AAAGCCAGAG CTTGGGCAAA ATGGTTGGGT GCGCGTAAAT ATGGGCTACC CAAAGTTTCT ACCAAATGAA ATTCCATTTG TTGCCGAAGA GCCAGAAGCT TTATATACCC TTGAGTTGGC AAATGACCAA AACATTAGTA TTGATGTGGT CAACATGGGT AACCCACACG CGGTCACTAT AGTTCCAGAT GTACTAACTG CTGATGTAGC TGGTATTGGT CCGCAAGTTG AATCACATAA ACGTTTCCCT GAGCGCGTTA ATGCTGGTTT CATGCAAGTG ATTGATGACA AGCATGTTCG CTTACGTGTA TTTGAACGTG GTGTGGGTGA AACACTTGCG TGTGGTACAG GAGCTTGTGC TGCTGCGGTA AGTGGCATGC GCCGTGGTCT ACTTGCGAAC TCTGTAGAAG TTGAACTTGC TGGTGGTAAG CTACAAATTG AATGGCAAGA AGGCGATGTG GTCTGGATGA CTGGTCCAAC AACTCATGTA TATGATGGAC GTTTGGATTT ACGTTATTTC CAAGGTTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T