SSGCID: Target Page

AcbaC.18851.a

electron transfer flavoprotein-ubiquinone oxidoreductase (EsvG)

CENTER ID:	AcbaC.18851.a
ORGANISM:	Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AcbaC.18851.a.B1.GE38891	full length		1	570

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AcbaC.18851.a.B1.PW37731	full length		1	570

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|470.1345.peg.3437
GenBank:	AKA33196.1
PGAT:	acinetobacter:ABUW_3502
RefSeq:	WP_000400008.1
UniProt:	V5VGV9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MEHIERESME FDVVIVGAGP AGLSAAIKIR QLAIENNLPD LSVCVVEKGS EVGAHILSGA VLEPRAINEL FPNWKEEGAP LNVPVTEDKT FFLLSETTSK EAPHWMVPKT MHNDGNYVIS LGNVVRWLGQ KAEELEVSIF PGFAASEVLY HEDGTVKGIQ TGDMGIGKDG EPTHNFTPGY ELHAKYTLFA EGCRGHLGKR LIAKYNLDKD ADPQHYGIGI KELWEIDPAK HKPGLVMHGA GWPLSETGSS GGWWLYHAEN NQVTLGMIVD LSYENPHMYP FMEMQRWKTH PLIKQYLEGG KRISYGARAV VKGGFNSLPK LTFPGGCLIG DDAGFLNFAK IKGSHTAMKS GMLCGEAVFE AIAAGVEKGG DLAVARVTEG EDLFVKELTS YTDKFNNSWL KEELYNARNF GPAMHKFGQW IGGAFNFIDQ NVFKVPFTLH DLKQDFAVLK TVDATSFKPT YPKPDGKLTF DRLSSVFISN TVHEENQPAH LKLTDPSIPV NVNLPKWDEP AQRYCPAGVY EIMENDDGSK RFQINAANCV HCKTCDIKDP SQNITWVTPE GGGGPNYPNM

NT Sequence

ATGGAACACA TCGAACGTGA ATCGATGGAG TTTGACGTTG TCATCGTAGG TGCAGGACCT GCAGGTCTAT CTGCTGCGAT TAAGATCCGT CAATTAGCGA TTGAAAATAA CTTACCCGAT CTTTCAGTTT GTGTAGTTGA AAAAGGCTCT GAAGTAGGTG CGCATATCTT ATCTGGTGCT GTACTTGAAC CGCGTGCTAT TAATGAACTT TTCCCGAACT GGAAAGAAGA AGGCGCGCCT CTTAACGTAC CTGTAACTGA AGACAAAACA TTCTTCTTGC TTTCAGAAAC TACTTCTAAA GAAGCACCTC ACTGGATGGT TCCTAAAACC ATGCACAATG ACGGGAACTA TGTTATCTCG CTAGGTAACG TTGTTCGCTG GTTAGGTCAA AAAGCTGAAG AATTAGAAGT TTCTATCTTC CCTGGCTTTG CTGCATCTGA AGTGCTTTAC CATGAAGACG GTACTGTAAA AGGTATTCAA ACTGGTGACA TGGGTATTGG TAAAGATGGC GAGCCTACAC ATAACTTTAC TCCGGGTTAT GAACTTCACG CTAAATACAC TCTCTTTGCT GAAGGCTGCC GTGGCCACTT AGGTAAACGC CTAATTGCAA AATATAATCT TGATAAAGAT GCAGATCCAC AACATTACGG GATCGGGATT AAAGAACTTT GGGAAATTGA CCCAGCAAAA CATAAACCGG GTCTAGTGAT GCACGGTGCT GGTTGGCCAT TGTCTGAAAC TGGTTCTTCT GGTGGTTGGT GGTTATACCA CGCTGAAAAC AACCAAGTGA CTTTGGGTAT GATTGTGGAC TTGTCTTATG AAAATCCACA CATGTACCCA TTCATGGAAA TGCAGCGCTG GAAAACTCAC CCACTCATCA AACAGTATTT AGAAGGTGGT AAACGTATTT CTTACGGCGC ACGTGCTGTA GTGAAAGGCG GTTTCAACTC TTTACCTAAA TTGACTTTCC CGGGTGGTTG CTTAATTGGT GATGATGCAG GTTTCTTAAA CTTTGCGAAA ATCAAAGGCT CACACACTGC CATGAAATCA GGCATGTTAT GTGGTGAAGC TGTATTTGAA GCAATTGCTG CCGGAGTTGA AAAAGGTGGT GATCTTGCGG TTGCTCGTGT AACCGAAGGC GAAGATTTAT TCGTTAAAGA ATTAACTTCT TACACAGACA AATTTAACAA CAGCTGGTTA AAAGAAGAGC TTTATAACGC TCGTAACTTT GGCCCTGCAA TGCATAAGTT TGGGCAATGG ATTGGTGGTG CATTTAACTT TATTGACCAA AACGTCTTTA AAGTACCATT CACTTTACAT GACTTGAAAC AAGACTTCGC TGTATTGAAA ACTGTTGATG CAACGAGCTT CAAGCCAACT TATCCAAAAC CAGATGGCAA ACTGACATTT GACCGTTTAT CTTCTGTGTT TATTTCAAAC ACTGTACACG AAGAAAACCA GCCAGCTCAC TTGAAGCTTA CTGATCCTTC AATTCCAGTA AATGTGAACT TACCGAAATG GGATGAGCCT GCTCAGCGCT ATTGCCCTGC AGGTGTTTAC GAAATCATGG AAAATGATGA CGGCTCTAAA CGCTTCCAGA TTAACGCAGC AAACTGTGTT CACTGTAAGA CCTGTGATAT TAAAGACCCA TCTCAAAATA TTACTTGGGT AACACCTGAG GGTGGTGGCG GTCCGAATTA CCCTAACATG TAA

Details for AcbaC.18851.a.B1.GE38891

HARVESTED ON:	1/29/2015
SEQUENCED ON:	1/15/2015
EXPECTED MW:	64kDa
OBSERVED MW:	64kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHME HIERESMEFD VVIVGAGPAG LSAAIKIRQL AIENNLPDLS VCVVEKGSEV GAHILSGAVL EPRAINELFP NWKEEGAPLN VPVTEDKTFF LLSETTSKEA PHWMVPKTMH NDGNYVISLG NVVRWLGQKA EELEVSIFPG FAASEVLYHE DGTVKGIQTG DMGIGKDGEP THNFTPGYEL HAKYTLFAEG CRGHLGKRLI AKYNLDKDAD PQHYGIGIKE LWEIDPAKHK PGLVMHGAGW PLSETGSSGG WWLYHAENNQ VTLGMIVDLS YENPHMYPFM EMQRWKTHPL IKQYLEGGKR ISYGARAVVK GGFNSLPKLT FPGGCLIGDD AGFLNFAKIK GSHTAMKSGM LCGEAVFEAI AAGVEKGGDL AVARVTEGED LFVKELTSYT DKFNNSWLKE ELYNARNFGP AMHKFGQWIG GAFNFIDQNV FKVPFTLHDL KQDFAVLKTV DATSFKPTYP KPDGKLTFDR LSSVFISNTV HEENQPAHLK LTDPSIPVNV NLPKWDEPAQ RYCPAGVYEI MENDDGSKRF QINAANCVHC KTCDIKDPSQ NITWVTPEGG GGPNYPNM

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaac acatcgaacg tgaatcgatg gagtttgacg ttgtcatcgt aggtgcagga cctgcaggtc tatctgctgc gattaagatc cgtcaattag cgattgaaaa taacttaccc gatctttcag tttgtgtagt tgaaaaaggc tctgaagtag gtgcgcatat cttatctggt gctgtacttg aaccgcgtgc tattaatgaa cttttcccga actggaaaga agaaggcgcg cctcttaacg tacctgtaac tgaagacaaa acattcttct tgctttcaga aactacttct aaagaagcac ctcactggat ggttcctaaa accatgcaca atgacgggaa ctatgttatc tcgctaggta acgttgttcg ctggttaggt caaaaagctg aagaattaga agtttctatc ttccctggct ttgctgcatc tgaagtgctt taccatgaag acggtactgt aaaaggtatt caaactggtg acatgggtat tggtaaagat ggcgagccta cacataactt tactccgggt tatgaacttc acgctaaata cactctcttt gctgaaggct gccgtggcca cttaggtaaa cgcctaattg caaaatataa tcttgataaa gatgcagatc cacaacatta cgggatcggg attaaagaac tttgggaaat tgacccagca aaacataaac cgggtctagt gatgcacggt gctggttggc cattgtctga aactggttct tctggtggtt ggtggttata ccacgctgaa aacaaccaag tgactttggg tatgattgtg gacttgtctt atgaaaatcc acacatgtac ccattcatgg aaatgcagcg ctggaaaact cacccactca tcaaacagta tttagaaggt ggtaaacgta tttcttacgg cgcacgtgct gtagtgaaag gcggtttcaa ctctttacct aaattgactt tcccgggtgg ttgcttaatt ggtgatgatg caggtttctt aaactttgcg aaaatcaaag gctcacacac tgccatgaaa tcaggcatgt tatgtggtga agctgtattt gaagcaattg ctgccggagt tgaaaaaggt ggtgatcttg cggttgctcg tgtaaccgaa ggcgaagatt tattcgttaa agaattaact tcttacacag acaaatttaa caacagctgg ttaaaagaag agctttataa cgctcgtaac tttggccctg caatgcataa gtttgggcaa tggattggtg gtgcatttaa ctttattgac caaaacgtct ttaaagtacc attcacttta catgacttga aacaagactt cgctgtattg aaaactgttg atgcaacgag cttcaagcca acttatccaa aaccagatgg caaactgaca tttgaccgtt tatcttctgt gtttatttca aacactgtac acgaagaaaa ccagccagct cacttgaagc ttactgatcc ttcaattcca gtaaatgtga acttaccgaa atgggatgag cctgctcagc gctattgccc tgcaggtgtt tacgaaatca tggaaaatga tgacggctct aaacgcttcc agattaacgc agcaaactgt gttcactgta agacctgtga tattaaagac ccatctcaaa atattacttg ggtaacacct gagggtggtg gcggtccgaa ttaccctaac atgtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat

Details for AcbaC.18851.a.B1.PW37731

PURIFICATION DATe:	6/10/2015
CONCENTRATION:	21.5mg/ml
OBSERVED MW:	68kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	8
VIAL VOLUME:	200µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGAAC ACATCGAACG TGAATCGATG GAGTTTGACG TTGTCATCGT AGGTGCAGGA CCTGCAGGTC TATCTGCTGC GATTAAGATC CGTCAATTAG CGATTGAAAA TAACTTACCC GATCTTTCAG TTTGTGTAGT TGAAAAAGGC TCTGAAGTAG GTGCGCATAT CTTATCTGGT GCTGTACTTG AACCGCGTGC TATTAATGAA CTTTTCCCGA ACTGGAAAGA AGAAGGCGCG CCTCTTAACG TACCTGTAAC TGAAGACAAA ACATTCTTCT TGCTTTCAGA AACTACTTCT AAAGAAGCAC CTCACTGGAT GGTTCCTAAA ACCATGCACA ATGACGGGAA CTATGTTATC TCGCTAGGTA ACGTTGTTCG CTGGTTAGGT CAAAAAGCTG AAGAATTAGA AGTTTCTATC TTCCCTGGCT TTGCTGCATC TGAAGTGCTT TACCATGAAG ACGGTACTGT AAAAGGTATT CAAACTGGTG ACATGGGTAT TGGTAAAGAT GGCGAGCCTA CACATAACTT TACTCCGGGT TATGAACTTC ACGCTAAATA CACTCTCTTT GCTGAAGGCT GCCGTGGCCA CTTAGGTAAA CGCCTAATTG CAAAATATAA TCTTGATAAA GATGCAGATC CACAACATTA CGGGATCGGG ATTAAAGAAC TTTGGGAAAT TGACCCAGCA AAACATAAAC CGGGTCTAGT GATGCACGGT GCTGGTTGGC CATTGTCTGA AACTGGTTCT TCTGGTGGTT GGTGGTTATA CCACGCTGAA AACAACCAAG TGACTTTGGG TATGATTGTG GACTTGTCTT ATGAAAATCC ACACATGTAC CCATTCATGG AAATGCAGCG CTGGAAAACT CACCCACTCA TCAAACAGTA TTTAGAAGGT GGTAAACGTA TTTCTTACGG CGCACGTGCT GTAGTGAAAG GCGGTTTCAA CTCTTTACCT AAATTGACTT TCCCGGGTGG TTGCTTAATT GGTGATGATG CAGGTTTCTT AAACTTTGCG AAAATCAAAG GCTCACACAC TGCCATGAAA TCAGGCATGT TATGTGGTGA AGCTGTATTT GAAGCAATTG CTGCCGGAGT TGAAAAAGGT GGTGATCTTG CGGTTGCTCG TGTAACCGAA GGCGAAGATT TATTCGTTAA AGAATTAACT TCTTACACAG ACAAATTTAA CAACAGCTGG TTAAAAGAAG AGCTTTATAA CGCTCGTAAC TTTGGCCCTG CAATGCATAA GTTTGGGCAA TGGATTGGTG GTGCATTTAA CTTTATTGAC CAAAACGTCT TTAAAGTACC ATTCACTTTA CATGACTTGA AACAAGACTT CGCTGTATTG AAAACTGTTG ATGCAACGAG CTTCAAGCCA ACTTATCCAA AACCAGATGG CAAACTGACA TTTGACCGTT TATCTTCTGT GTTTATTTCA AACACTGTAC ACGAAGAAAA CCAGCCAGCT CACTTGAAGC TTACTGATCC TTCAATTCCA GTAAATGTGA ACTTACCGAA ATGGGATGAG CCTGCTCAGC GCTATTGCCC TGCAGGTGTT TACGAAATCA TGGAAAATGA TGACGGCTCT AAACGCTTCC AGATTAACGC AGCAAACTGT GTTCACTGTA AGACCTGTGA TATTAAAGAC CCATCTCAAA ATATTACTTG GGTAACACCT GAGGGTGGTG GCGGTCCGAA TTACCCTAAC ATGTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT