AcbaC.18844.a

ribonucleoside-diphosphate reductase alpha subunit

CENTER ID: AcbaC.18844.a
ORGANISM: Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AcbaC.18844.a.B1.GE38886 full length 1 944
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AcbaC.18844.a.B1.PS02361 full length 1 944

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|470.1345.peg.3128
GenBank: AKA32890.1
PGAT: acinetobacter:ABUW_3183
RefSeq: WP_000111540.1
UniProt: V5VGK0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSVITSTPGQ IQVIKRTGDV AAFDAEKISV AIGKAFLAVE GQQSADSSRI HDRITQLTEM VLNTFTRRLP SGGTIHIEEI QDQVELALMR TGEHKVARAY VIYRDQRASA RKDTNSNHHP TLQVTDANGQ LQPLDLSALQ ATVNRAAEGL EGIDVQAIID ETVKNLYNGV KESDIATTMM MATRTRIEQE PNYTYVTARL LRDELVSTGL AFLGLPADTA ENNALEAFLK KGVELDLLSP DLLKFDLEKL AAAIQPERSN QFTYLGLQTL FDRYFIHSNG VRFELPQLFF MRVSMGLALN EQDKEERAIE FYNLLSSFDY MASTPTLFNS GTLRPQLSSC YLTTIGDDLY DIYGAMRDNA MLSKWAGGLG NDWTPVRALN SYIKGTNGKS QGVVPFLKVA NDTAVAVNQG GKRKGAVCAY LETWHLDIEE FLELRKNTGD DRRRTHDMNT ANWVPDLFMQ RVFEDGEWTL FTPSETPDLH DLTGAEFAER YAYYESVAKE QNMLHKKVRA KDLWRKMLSM LFETGHPWIT FKDVCNLRSP QQHVGVVHSS NLCTEITLNT NQDEIAVCNL GSINLVQHVK GGVLDREKLA RTVKTAVRML DNVIDINYYA VPQAKNSNLK HRPVGMGIMG FQDALYEMGM AYGSDEAVNF ADESMEVISY YAIQTSSDLA VERGAYSTFK GSLWDQGILP IDSLEIVAKS RPERMFEVDR TQRLDWDSLR AKVQKDGMRN SNVMAIAPTA TISNICGVSQ SIEPTFQNLY VKSNLSGEFT VINPYLVRAL KERGLWDTVM VNDLKHFEGS VQKIARIPEE LKAIFATAFE VEPRWIVDAA SRRQKWIDQA QSLNLYISGA NGKKLDITYK MAWLRGLKTT YYLRALGATS AEKSTINTGA LNAVKPATVE AAAPAAAPVV EAKKPEAVEE DGFTQAAPVP MACSIDNPDC EACQ
NT Sequence
ATGAGCGTAA TTACTTCGAC TCCCGGTCAA ATTCAGGTGA TTAAACGCAC CGGAGATGTT GCTGCATTTG ATGCAGAGAA AATTTCTGTT GCGATTGGTA AAGCTTTTCT TGCTGTGGAA GGTCAACAAA GTGCGGATTC GAGCCGTATT CACGACCGTA TTACCCAACT GACGGAAATG GTACTGAACA CTTTCACTCG CCGTTTACCT TCTGGCGGTA CCATTCATAT TGAAGAGATT CAAGATCAAG TTGAACTTGC ACTGATGCGT ACTGGGGAGC ACAAAGTCGC TCGTGCTTAC GTTATCTATC GCGATCAACG CGCAAGTGCC CGTAAAGACA CAAATTCTAA TCATCACCCT ACCTTGCAAG TTACCGATGC AAACGGTCAG CTTCAACCAC TTGATTTAAG TGCACTGCAA GCAACTGTAA ATCGAGCTGC TGAAGGCTTA GAAGGTATTG ATGTTCAAGC AATTATCGAT GAAACCGTTA AAAACTTATA TAACGGCGTA AAAGAAAGCG ATATTGCTAC CACCATGATG ATGGCAACAC GTACACGTAT TGAACAAGAA CCTAACTATA CATACGTAAC TGCACGTTTG CTTCGTGATG AGTTGGTAAG CACTGGCTTA GCATTCTTAG GTTTACCGGC CGATACAGCT GAAAACAATG CACTTGAAGC ATTCTTGAAA AAAGGTGTAG AGCTTGATTT GCTTTCACCG GACTTGCTTA AGTTCGATCT AGAAAAACTA GCAGCAGCGA TTCAACCGGA ACGTTCTAAC CAGTTTACCT ATTTAGGTTT ACAGACTCTA TTTGACCGTT ACTTCATCCA TTCAAATGGC GTTCGCTTCG AATTACCACA ATTATTCTTC ATGCGTGTGT CAATGGGTCT TGCACTTAAT GAGCAAGACA AAGAAGAGCG TGCAATCGAG TTCTATAACT TGTTGTCTAG CTTTGACTAT ATGGCATCTA CGCCTACCCT GTTTAACTCA GGTACATTAC GTCCACAGCT TTCAAGCTGT TACTTAACAA CGATTGGCGA TGACCTTTAT GACATTTATG GTGCAATGCG TGACAACGCG ATGCTCTCTA AATGGGCAGG TGGTTTAGGT AACGACTGGA CACCTGTACG TGCCTTGAAC TCTTATATTA AAGGTACAAA CGGTAAATCT CAGGGTGTTG TTCCGTTCCT TAAAGTTGCG AACGATACAG CCGTTGCGGT AAACCAAGGT GGTAAGCGTA AAGGTGCGGT ATGTGCGTAC TTAGAAACTT GGCACTTAGA CATTGAAGAA TTCTTAGAAC TTCGTAAAAA CACGGGTGAT GACCGTCGTC GTACTCACGA CATGAACACT GCAAACTGGG TTCCTGACCT ATTTATGCAA CGTGTGTTTG AAGATGGCGA ATGGACACTA TTCACTCCGT CTGAAACTCC AGACCTACAC GATTTAACTG GTGCTGAGTT TGCTGAACGC TATGCATACT ATGAGTCTGT TGCGAAAGAA CAGAACATGT TGCACAAGAA AGTACGTGCG AAAGACTTAT GGCGTAAAAT GCTTTCTATG TTGTTCGAAA CTGGTCACCC TTGGATCACG TTTAAAGATG TTTGTAATTT ACGTTCACCA CAACAACACG TTGGCGTAGT TCACTCATCT AACCTATGTA CTGAAATTAC GTTAAACACC AACCAAGACG AAATTGCGGT ATGTAACTTA GGTTCGATCA ACCTTGTTCA ACACGTTAAA GGTGGCGTAT TAGACCGTGA GAAGTTAGCG CGTACTGTTA AAACAGCAGT TCGTATGCTC GATAACGTTA TCGACATTAA CTACTACGCT GTACCACAAG CGAAGAACTC TAACTTGAAA CACCGCCCTG TTGGTATGGG TATCATGGGC TTCCAAGATG CACTTTACGA AATGGGTATG GCTTACGGTT CTGATGAAGC TGTGAACTTT GCTGATGAGT CAATGGAAGT GATTAGCTAC TATGCAATCC AAACTTCAAG TGACTTGGCT GTTGAACGTG GTGCTTACTC AACATTTAAA GGTTCATTGT GGGATCAAGG CATCCTTCCA ATCGACTCTT TAGAAATTGT TGCGAAATCT CGTCCAGAAC GTATGTTTGA AGTTGACCGT ACTCAACGTT TAGATTGGGA CAGTTTACGT GCCAAAGTTC AAAAAGACGG TATGCGTAAC TCAAACGTAA TGGCGATTGC TCCAACTGCA ACCATTTCGA ACATTTGTGG TGTTTCTCAG TCTATTGAGC CAACATTCCA AAACTTATAT GTGAAATCTA ACTTGTCTGG TGAGTTCACT GTAATTAACC CTTACCTTGT TCGCGCATTA AAAGAACGTG GTCTTTGGGA CACAGTGATG GTTAATGACC TCAAACACTT TGAAGGTTCA GTACAAAAAA TTGCTCGTAT TCCTGAAGAA TTAAAAGCAA TCTTTGCAAC TGCGTTTGAA GTTGAACCAC GCTGGATTGT TGATGCTGCA TCACGCCGCC AAAAATGGAT TGATCAAGCA CAATCTCTTA ACCTTTACAT CTCTGGTGCA AACGGTAAGA AACTTGACAT CACTTATAAG ATGGCATGGT TACGTGGTCT TAAGACGACT TATTACCTCC GTGCGTTAGG TGCAACATCT GCTGAAAAAT CTACAATTAA CACAGGTGCG TTAAACGCGG TTAAACCTGC AACTGTAGAA GCAGCAGCAC CTGCGGCAGC TCCAGTCGTA GAGGCGAAGA AGCCTGAAGC AGTTGAAGAA GACGGCTTCA CTCAAGCAGC TCCGGTTCCT ATGGCTTGTT CAATCGATAA CCCAGATTGC GAAGCTTGCC AATAA
Details for AcbaC.18844.a.B1.GE38886
HARVESTED ON: 1/29/2015
SEQUENCED ON: 1/15/2015
EXPECTED MW: 106kDa
OBSERVED MW: 100kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMS VITSTPGQIQ VIKRTGDVAA FDAEKISVAI GKAFLAVEGQ QSADSSRIHD RITQLTEMVL NTFTRRLPSG GTIHIEEIQD QVELALMRTG EHKVARAYVI YRDQRASARK DTNSNHHPTL QVTDANGQLQ PLDLSALQAT VNRAAEGLEG IDVQAIIDET VKNLYNGVKE SDIATTMMMA TRTRIEQEPN YTYVTARLLR DELVSTGLAF LGLPADTAEN NALEAFLKKG VELDLLSPDL LKFDLEKLAA AIQPERSNQF TYLGLQTLFD RYFIHSNGVR FELPQLFFMR VSMGLALNEQ DKEERAIEFY NLLSSFDYMA STPTLFNSGT LRPQLSSCYL TTIGDDLYDI YGAMRDNAML SKWAGGLGND WTPVRALNSY IKGTNGKSQG VVPFLKVAND TAVAVNQGGK RKGAVCAYLE TWHLDIEEFL ELRKNTGDDR RRTHDMNTAN WVPDLFMQRV FEDGEWTLFT PSETPDLHDL TGAEFAERYA YYESVAKEQN MLHKKVRAKD LWRKMLSMLF ETGHPWITFK DVCNLRSPQQ HVGVVHSSNL CTEITLNTNQ DEIAVCNLGS INLVQHVKGG VLDREKLART VKTAVRMLDN VIDINYYAVP QAKNSNLKHR PVGMGIMGFQ DALYEMGMAY GSDEAVNFAD ESMEVISYYA IQTSSDLAVE RGAYSTFKGS LWDQGILPID SLEIVAKSRP ERMFEVDRTQ RLDWDSLRAK VQKDGMRNSN VMAIAPTATI SNICGVSQSI EPTFQNLYVK SNLSGEFTVI NPYLVRALKE RGLWDTVMVN DLKHFEGSVQ KIARIPEELK AIFATAFEVE PRWIVDAASR RQKWIDQAQS LNLYISGANG KKLDITYKMA WLRGLKTTYY LRALGATSAE KSTINTGALN AVKPATVEAA APAAAPVVEA KKPEAVEEDG FTQAAPVPMA CSIDNPDCEA CQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagcg taattacttc gactcccggt caaattcagg tgattaaacg caccggagat gttgctgcat ttgatgcaga gaaaatttct gttgcgattg gtaaagcttt tcttgctgtg gaaggtcaac aaagtgcgga ttcgagccgt attcacgacc gtattaccca actgacggaa atggtactga acactttcac tcgccgttta ccttctggcg gtaccattca tattgaagag attcaagatc aagttgaact tgcactgatg cgtactgggg agcacaaagt cgctcgtgct tacgttatct atcgcgatca acgcgcaagt gcccgtaaag acacaaattc taatcatcac cctaccttgc aagttaccga tgcaaacggt cagcttcaac cacttgattt aagtgcactg caagcaactg taaatcgagc tgctgaaggc ttagaaggta ttgatgttca agcaattatc gatgaaaccg ttaaaaactt atataacggc gtaaaagaaa gcgatattgc taccaccatg atgatggcaa cacgtacacg tattgaacaa gaacctaact atacatacgt aactgcacgt ttgcttcgtg atgagttggt aagcactggc ttagcattct taggtttacc ggccgataca gctgaaaaca atgcacttga agcattcttg aaaaaaggtg tagagcttga tttgctttca ccggacttgc ttaagttcga tctagaaaaa ctagcagcag cgattcaacc ggaacgttct aaccagttta cctatttagg tttacagact ctatttgacc gttacttcat ccattcaaat ggcgttcgct tcgaattacc acaattattc ttcatgcgtg tgtcaatggg tcttgcactt aatgagcaag acaaagaaga gcgtgcaatc gagttctata acttgttgtc tagctttgac tatatggcat ctacgcctac cctgtttaac tcaggtacat tacgtccaca gctttcaagc tgttacttaa caacgattgg cgatgacctt tatgacattt atggtgcaat gcgtgacaac gcgatgctct ctaaatgggc aggtggttta ggtaacgact ggacacctgt acgtgccttg aactcttata ttaaaggtac aaacggtaaa tctcagggtg ttgttccgtt ccttaaagtt gcgaacgata cagccgttgc ggtaaaccaa ggtggtaagc gtaaaggtgc ggtatgtgcg tacttagaaa cttggcactt agacattgaa gaattcttag aacttcgtaa aaacacgggt gatgaccgtc gtcgtactca cgacatgaac actgcaaact gggttcctga cctatttatg caacgtgtgt ttgaagatgg cgaatggaca ctattcactc cgtctgaaac tccagaccta cacgatttaa ctggtgctga gtttgctgaa cgctatgcat actatgagtc tgttgcgaaa gaacagaaca tgttgcacaa gaaagtacgt gcgaaagact tatggcgtaa aatgctttct atgttgttcg aaactggtca cccttggatc acgtttaaag atgtttgtaa tttacgttca ccacaacaac acgttggcgt agttcactca tctaacctat gtactgaaat tacgttaaac accaaccaag acgaaattgc ggtatgtaac ttaggttcga tcaaccttgt tcaacacgtt aaaggtggcg tattagaccg tgagaagtta gcgcgtactg ttaaaacagc agttcgtatg ctcgataacg ttatcgacat taactactac gctgtaccac aagcgaagaa ctctaacttg aaacaccgcc ctgttggtat gggtatcatg ggcttccaag atgcacttta cgaaatgggt atggcttacg gttctgatga agctgtgaac tttgctgatg agtcaatgga agtgattagc tactatgcaa tccaaacttc aagtgacttg gctgttgaac gtggtgctta ctcaacattt aaaggttcat tgtgggatca aggcatcctt ccaatcgact ctttagaaat tgttgcgaaa tctcgtccag aacgtatgtt tgaagttgac cgtactcaac gtttagattg ggacagttta cgtgccaaag ttcaaaaaga cggtatgcgt aactcaaacg taatggcgat tgctccaact gcaaccattt cgaacatttg tggtgtttct cagtctattg agccaacatt ccaaaactta tatgtgaaat ctaacttgtc tggtgagttc actgtaatta acccttacct tgttcgcgca ttaaaagaac gtggtctttg ggacacagtg atggttaatg acctcaaaca ctttgaaggt tcagtacaaa aaattgctcg tattcctgaa gaattaaaag caatctttgc aactgcgttt gaagttgaac cacgctggat tgttgatgct gcatcacgcc gccaaaaatg gattgatcaa gcacaatctc ttaaccttta catctctggt gcaaacggta agaaacttga catcacttat aagatggcat ggttacgtgg tcttaagacg acttattacc tccgtgcgtt aggtgcaaca tctgctgaaa aatctacaat taacacaggt gcgttaaacg cggttaaacc tgcaactgta gaagcagcag cacctgcggc agctccagtc gtagaggcga agaagcctga agcagttgaa gaagacggct tcactcaagc agctccggtt cctatggctt gttcaatcga taacccagat tgcgaagctt gccaatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for AcbaC.18844.a.B1.PS02361
PURIFICATION DATe: 3/18/2015
CONCENTRATION: 20mg/ml
OBSERVED MW: 110kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 110µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMS VITSTPGQIQ VIKRTGDVAA FDAEKISVAI GKAFLAVEGQ QSADSSRIHD RITQLTEMVL NTFTRRLPSG GTIHIEEIQD QVELALMRTG EHKVARAYVI YRDQRASARK DTNSNHHPTL QVTDANGQLQ PLDLSALQAT VNRAAEGLEG IDVQAIIDET VKNLYNGVKE SDIATTMMMA TRTRIEQEPN YTYVTARLLR DELVSTGLAF LGLPADTAEN NALEAFLKKG VELDLLSPDL LKFDLEKLAA AIQPERSNQF TYLGLQTLFD RYFIHSNGVR FELPQLFFMR VSMGLALNEQ DKEERAIEFY NLLSSFDYMA STPTLFNSGT LRPQLSSCYL TTIGDDLYDI YGAMRDNAML SKWAGGLGND WTPVRALNSY IKGTNGKSQG VVPFLKVAND TAVAVNQGGK RKGAVCAYLE TWHLDIEEFL ELRKNTGDDR RRTHDMNTAN WVPDLFMQRV FEDGEWTLFT PSETPDLHDL TGAEFAERYA YYESVAKEQN MLHKKVRAKD LWRKMLSMLF ETGHPWITFK DVCNLRSPQQ HVGVVHSSNL CTEITLNTNQ DEIAVCNLGS INLVQHVKGG VLDREKLART VKTAVRMLDN VIDINYYAVP QAKNSNLKHR PVGMGIMGFQ DALYEMGMAY GSDEAVNFAD ESMEVISYYA IQTSSDLAVE RGAYSTFKGS LWDQGILPID SLEIVAKSRP ERMFEVDRTQ RLDWDSLRAK VQKDGMRNSN VMAIAPTATI SNICGVSQSI EPTFQNLYVK SNLSGEFTVI NPYLVRALKE RGLWDTVMVN DLKHFEGSVQ KIARIPEELK AIFATAFEVE PRWIVDAASR RQKWIDQAQS LNLYISGANG KKLDITYKMA WLRGLKTTYY LRALGATSAE KSTINTGALN AVKPATVEAA APAAAPVVEA KKPEAVEEDG FTQAAPVPMA CSIDNPDCEA CQ
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGCG TAATTACTTC GACTCCCGGT CAAATTCAGG TGATTAAACG CACCGGAGAT GTTGCTGCAT TTGATGCAGA GAAAATTTCT GTTGCGATTG GTAAAGCTTT TCTTGCTGTG GAAGGTCAAC AAAGTGCGGA TTCGAGCCGT ATTCACGACC GTATTACCCA ACTGACGGAA ATGGTACTGA ACACTTTCAC TCGCCGTTTA CCTTCTGGCG GTACCATTCA TATTGAAGAG ATTCAAGATC AAGTTGAACT TGCACTGATG CGTACTGGGG AGCACAAAGT CGCTCGTGCT TACGTTATCT ATCGCGATCA ACGCGCAAGT GCCCGTAAAG ACACAAATTC TAATCATCAC CCTACCTTGC AAGTTACCGA TGCAAACGGT CAGCTTCAAC CACTTGATTT AAGTGCACTG CAAGCAACTG TAAATCGAGC TGCTGAAGGC TTAGAAGGTA TTGATGTTCA AGCAATTATC GATGAAACCG TTAAAAACTT ATATAACGGC GTAAAAGAAA GCGATATTGC TACCACCATG ATGATGGCAA CACGTACACG TATTGAACAA GAACCTAACT ATACATACGT AACTGCACGT TTGCTTCGTG ATGAGTTGGT AAGCACTGGC TTAGCATTCT TAGGTTTACC GGCCGATACA GCTGAAAACA ATGCACTTGA AGCATTCTTG AAAAAAGGTG TAGAGCTTGA TTTGCTTTCA CCGGACTTGC TTAAGTTCGA TCTAGAAAAA CTAGCAGCAG CGATTCAACC GGAACGTTCT AACCAGTTTA CCTATTTAGG TTTACAGACT CTATTTGACC GTTACTTCAT CCATTCAAAT GGCGTTCGCT TCGAATTACC ACAATTATTC TTCATGCGTG TGTCAATGGG TCTTGCACTT AATGAGCAAG ACAAAGAAGA GCGTGCAATC GAGTTCTATA ACTTGTTGTC TAGCTTTGAC TATATGGCAT CTACGCCTAC CCTGTTTAAC TCAGGTACAT TACGTCCACA GCTTTCAAGC TGTTACTTAA CAACGATTGG CGATGACCTT TATGACATTT ATGGTGCAAT GCGTGACAAC GCGATGCTCT CTAAATGGGC AGGTGGTTTA GGTAACGACT GGACACCTGT ACGTGCCTTG AACTCTTATA TTAAAGGTAC AAACGGTAAA TCTCAGGGTG TTGTTCCGTT CCTTAAAGTT GCGAACGATA CAGCCGTTGC GGTAAACCAA GGTGGTAAGC GTAAAGGTGC GGTATGTGCG TACTTAGAAA CTTGGCACTT AGACATTGAA GAATTCTTAG AACTTCGTAA AAACACGGGT GATGACCGTC GTCGTACTCA CGACATGAAC ACTGCAAACT GGGTTCCTGA CCTATTTATG CAACGTGTGT TTGAAGATGG CGAATGGACA CTATTCACTC CGTCTGAAAC TCCAGACCTA CACGATTTAA CTGGTGCTGA GTTTGCTGAA CGCTATGCAT ACTATGAGTC TGTTGCGAAA GAACAGAACA TGTTGCACAA GAAAGTACGT GCGAAAGACT TATGGCGTAA AATGCTTTCT ATGTTGTTCG AAACTGGTCA CCCTTGGATC ACGTTTAAAG ATGTTTGTAA TTTACGTTCA CCACAACAAC ACGTTGGCGT AGTTCACTCA TCTAACCTAT GTACTGAAAT TACGTTAAAC ACCAACCAAG ACGAAATTGC GGTATGTAAC TTAGGTTCGA TCAACCTTGT TCAACACGTT AAAGGTGGCG TATTAGACCG TGAGAAGTTA GCGCGTACTG TTAAAACAGC AGTTCGTATG CTCGATAACG TTATCGACAT TAACTACTAC GCTGTACCAC AAGCGAAGAA CTCTAACTTG AAACACCGCC CTGTTGGTAT GGGTATCATG GGCTTCCAAG ATGCACTTTA CGAAATGGGT ATGGCTTACG GTTCTGATGA AGCTGTGAAC TTTGCTGATG AGTCAATGGA AGTGATTAGC TACTATGCAA TCCAAACTTC AAGTGACTTG GCTGTTGAAC GTGGTGCTTA CTCAACATTT AAAGGTTCAT TGTGGGATCA AGGCATCCTT CCAATCGACT CTTTAGAAAT TGTTGCGAAA TCTCGTCCAG AACGTATGTT TGAAGTTGAC CGTACTCAAC GTTTAGATTG GGACAGTTTA CGTGCCAAAG TTCAAAAAGA CGGTATGCGT AACTCAAACG TAATGGCGAT TGCTCCAACT GCAACCATTT CGAACATTTG TGGTGTTTCT CAGTCTATTG AGCCAACATT CCAAAACTTA TATGTGAAAT CTAACTTGTC TGGTGAGTTC ACTGTAATTA ACCCTTACCT TGTTCGCGCA TTAAAAGAAC GTGGTCTTTG GGACACAGTG ATGGTTAATG ACCTCAAACA CTTTGAAGGT TCAGTACAAA AAATTGCTCG TATTCCTGAA GAATTAAAAG CAATCTTTGC AACTGCGTTT GAAGTTGAAC CACGCTGGAT TGTTGATGCT GCATCACGCC GCCAAAAATG GATTGATCAA GCACAATCTC TTAACCTTTA CATCTCTGGT GCAAACGGTA AGAAACTTGA CATCACTTAT AAGATGGCAT GGTTACGTGG TCTTAAGACG ACTTATTACC TCCGTGCGTT AGGTGCAACA TCTGCTGAAA AATCTACAAT TAACACAGGT GCGTTAAACG CGGTTAAACC TGCAACTGTA GAAGCAGCAG CACCTGCGGC AGCTCCAGTC GTAGAGGCGA AGAAGCCTGA AGCAGTTGAA GAAGACGGCT TCACTCAAGC AGCTCCGGTT CCTATGGCTT GTTCAATCGA TAACCCAGAT TGCGAAGCTT GCCAATGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT