AcbaC.18487.a

NADH dehydrogenase I chain G

CENTER ID: AcbaC.18487.a
ORGANISM: Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AcbaC.18487.a.B1.GE38881 full length 1 894
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|470.1345.peg.3117
GenBank: AKA32879.1
PGAT: acinetobacter:ABUW_3172
RefSeq: WP_000190957.1
UniProt: A0A098SGG9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MATIHVDGKS YEVNGSENLL QACLSLGIDI PYFCWHPSLG SVGSCRQCAV TQYANPEDTR GRLVMSCMTP ASDNTYISIE DKEAVDFRES IVEFLMTNHP HDCPVCEEGG HCHLQDMTVM TGHDRRRYRF TKRTHYNQEL GSFIAHEMNR CIACYRCVRY YKDYAGGTDF GVYANASRVY FGRPESGTLE SEFSGNLTEV CPTGVFTDKT HSERYNRKWD MQYAPSVCHG CSSGCNISPG ERYGEIRRVE NRFNGSVNQY FLCDKGRFGT GYVNRADRPR QPQFRNGETV ATVSVDQALD QTIAKLEGKK VLGIGSPRAS LESNYALREL VGQANYSTGV AQKEQSLVEL AASIMQTEGI YNPNMREIES YDAVLILGED LTQTAPRMAL SVRQAAKNKA REMAAKTRTP DWLAEPVQRI GQEAKSPIYI LAATQTRLAD VATGEVVASP NDIARLGFAV AAAVKGETVN GLDEDAKAFA QTIADTLKAA NKPLIIAGTS LQDASIMEAA AELTQNLGSK AGLSLVVPEV NSMGLALFGG LSLEQAFAQD YDTLVIVEND LFRRLPAAQV KAALDKAETV IVLDHSETET VKQADIVLSA ASFAEGDGTV VSQEGRAQRF YQVYDPSYYK PEYAIKESWR WLHAIETGLK GKPIDWTVLD DVIETIVKNV PVLEAIQDVA PDAGYRVHGL KIAREPRRYS GRTAMRAPLS VHEPKQPTDP DSALTFSMEG YVGPQKASSL VPFAWAPGWN SPQSWNKYQD EVGGHLKGGD SGVRLFDRLA KRPARSYVAP TAVVANPESF RLVPMHHIFG SGEFTIKTPA METRIPEAVF AVGEQDATRL NLQEGQRITV KAGETTVSLP VQVIEYLPTG YIGYPVGLAP TVSLAEPVSV ALGV
NT Sequence
ATGGCTACAA TTCATGTCGA TGGAAAATCG TACGAAGTCA ATGGATCAGA AAACTTGCTA CAAGCATGTT TATCCCTTGG CATTGATATT CCGTATTTTT GTTGGCACCC ATCCTTAGGT TCTGTTGGTT CATGTCGTCA ATGTGCCGTA ACTCAGTATG CAAATCCTGA AGATACGCGC GGACGTCTTG TGATGTCATG TATGACACCA GCATCTGACA ATACCTATAT TTCGATTGAA GACAAAGAAG CGGTAGATTT CCGTGAATCT ATTGTTGAGT TCTTAATGAC CAACCACCCA CACGACTGTC CAGTGTGTGA AGAGGGTGGT CACTGTCATT TACAAGATAT GACTGTAATG ACAGGTCACG ACCGTCGTCG CTATCGTTTT ACTAAGCGTA CACATTACAA CCAAGAACTA GGCTCATTCA TTGCACATGA AATGAACCGT TGTATTGCTT GTTATCGTTG TGTTCGTTAC TACAAGGATT ATGCAGGCGG TACAGACTTT GGTGTATATG CCAATGCATC ACGTGTTTAC TTTGGTCGTC CAGAATCAGG TACTTTAGAG TCTGAATTCT CTGGTAACTT GACTGAAGTA TGTCCAACTG GTGTATTTAC CGATAAGACT CATTCAGAGC GCTATAACCG TAAATGGGAT ATGCAATATG CACCAAGCGT GTGTCATGGC TGTTCTTCAG GTTGTAACAT CTCTCCGGGT GAGCGTTATG GCGAAATCCG TCGTGTAGAA AACCGTTTCA ATGGTTCGGT TAACCAATAC TTCCTTTGTG ACAAAGGCCG TTTTGGCACA GGTTATGTAA ACCGTGCAGA TCGTCCACGT CAACCTCAGT TCCGTAATGG CGAAACAGTA GCTACTGTTT CTGTTGACCA AGCGTTGGAC CAAACAATTG CGAAACTTGA AGGTAAAAAA GTTCTAGGTA TCGGTTCGCC ACGTGCAAGC TTAGAATCAA ATTACGCATT GCGTGAACTA GTGGGCCAAG CAAACTACTC AACAGGTGTT GCACAAAAAG AGCAAAGCCT CGTTGAACTT GCTGCTTCAA TCATGCAAAC AGAGGGTATT TACAACCCAA ACATGCGTGA AATTGAAAGC TACGATGCAG TATTAATTTT GGGTGAAGAT TTAACTCAAA CTGCTCCACG TATGGCATTG TCAGTTCGTC AAGCTGCAAA AAATAAAGCT CGCGAAATGG CTGCAAAAAC ACGTACACCG GACTGGTTGG CTGAGCCTGT ACAACGTATT GGTCAGGAAG CGAAATCTCC GATCTATATC TTGGCTGCAA CTCAAACTCG TCTTGCAGAT GTTGCGACAG GTGAAGTTGT TGCATCTCCA AATGATATTG CGCGTTTAGG TTTTGCAGTT GCTGCGGCAG TTAAAGGTGA AACAGTTAAT GGTTTAGATG AAGATGCAAA AGCATTTGCT CAAACTATTG CCGACACTTT AAAAGCAGCA AACAAACCGT TAATTATTGC GGGTACGAGC TTGCAAGATG CTTCAATTAT GGAAGCGGCA GCAGAGTTAA CGCAAAACCT TGGCTCAAAA GCTGGTTTAA GCCTTGTTGT TCCTGAAGTG AACTCTATGG GCTTGGCATT ATTTGGTGGC TTAAGCCTTG AACAAGCATT TGCTCAAGAC TACGACACAC TTGTTATTGT TGAAAATGAC TTATTCCGTC GTTTACCGGC CGCTCAAGTT AAAGCAGCAC TTGATAAAGC TGAAACAGTA ATCGTGCTTG ACCACAGTGA AACTGAAACT GTGAAACAGG CTGATATTGT ACTTTCAGCA GCAAGCTTTG CTGAAGGTGA TGGTACTGTC GTAAGCCAAG AAGGTCGTGC ACAACGCTTC TACCAAGTTT ACGATCCAAG CTACTACAAA CCTGAATACG CGATTAAAGA ATCTTGGCGT TGGTTGCATG CCATTGAAAC TGGCCTTAAA GGCAAGCCAA TTGATTGGAC AGTACTTGAC GACGTAATCG AAACAATCGT GAAGAACGTT CCAGTACTTG AAGCAATTCA AGATGTTGCG CCAGACGCTG GCTACCGTGT ACATGGTTTG AAAATTGCTC GTGAACCACG TCGTTATTCT GGTCGTACAG CAATGCGTGC GCCTTTATCG GTTCATGAAC CTAAACAACC GACAGATCCA GACTCAGCAT TAACATTCTC TATGGAAGGT TATGTAGGTC CGCAAAAAGC TTCTTCGCTT GTACCATTTG CGTGGGCACC AGGTTGGAAC TCTCCACAAT CTTGGAACAA ATACCAAGAT GAAGTGGGTG GTCACTTAAA AGGTGGTGAT TCAGGCGTTC GTTTATTCGA CCGTTTAGCA AAACGTCCAG CACGTAGCTA TGTCGCTCCA ACAGCTGTAG TTGCAAATCC TGAAAGCTTC CGTCTTGTAC CAATGCACCA TATCTTTGGT TCTGGCGAAT TCACAATTAA AACACCTGCA ATGGAAACTC GTATTCCTGA AGCTGTATTT GCTGTGGGCG AACAAGACGC AACACGTTTG AATCTACAAG AAGGCCAACG TATTACAGTA AAAGCAGGCG AAACAACTGT GAGCTTACCT GTACAAGTAA TTGAATATTT ACCTACAGGT TATATTGGTT ACCCAGTTGG TTTAGCTCCG ACAGTATCTT TAGCTGAACC TGTTTCAGTG GCATTAGGAG TGTAA
Details for AcbaC.18487.a.B1.GE38881
HARVESTED ON: 1/29/2015
SEQUENCED ON: 1/15/2015
EXPECTED MW: 99kDa
OBSERVED MW: 99kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMA TIHVDGKSYE VNGSENLLQA CLSLGIDIPY FCWHPSLGSV GSCRQCAVTQ YANPEDTRGR LVMSCMTPAS DNTYISIEDK EAVDFRESIV EFLMTNHPHD CPVCEEGGHC HLQDMTVMTG HDRRRYRFTK RTHYNQELGS FIAHEMNRCI ACYRCVRYYK DYAGGTDFGV YANASRVYFG RPESGTLESE FSGNLTEVCP TGVFTDKTHS ERYNRKWDMQ YAPSVCHGCS SGCNISPGER YGEIRRVENR FNGSVNQYFL CDKGRFGTGY VNRADRPRQP QFRNGETVAT VSVDQALDQT IAKLEGKKVL GIGSPRASLE SNYALRELVG QANYSTGVAQ KEQSLVELAA SIMQTEGIYN PNMREIESYD AVLILGEDLT QTAPRMALSV RQAAKNKARE MAAKTRTPDW LAEPVQRIGQ EAKSPIYILA ATQTRLADVA TGEVVASPND IARLGFAVAA AVKGETVNGL DEDAKAFAQT IADTLKAANK PLIIAGTSLQ DASIMEAAAE LTQNLGSKAG LSLVVPEVNS MGLALFGGLS LEQAFAQDYD TLVIVENDLF RRLPAAQVKA ALDKAETVIV LDHSETETVK QADIVLSAAS FAEGDGTVVS QEGRAQRFYQ VYDPSYYKPE YAIKESWRWL HAIETGLKGK PIDWTVLDDV IETIVKNVPV LEAIQDVAPD AGYRVHGLKI AREPRRYSGR TAMRAPLSVH EPKQPTDPDS ALTFSMEGYV GPQKASSLVP FAWAPGWNSP QSWNKYQDEV GGHLKGGDSG VRLFDRLAKR PARSYVAPTA VVANPESFRL VPMHHIFGSG EFTIKTPAME TRIPEAVFAV GEQDATRLNL QEGQRITVKA GETTVSLPVQ VIEYLPTGYI GYPVGLAPTV SLAEPVSVAL GV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcta caattcatgt cgatggaaaa tcgtacgaag tcaatggatc agaaaacttg ctacaagcat gtttatccct tggcattgat attccgtatt tttgttggca cccatcctta ggttctgttg gttcatgtcg tcaatgtgcc gtaactcagt atgcaaatcc tgaagatacg cgcggacgtc ttgtgatgtc atgtatgaca ccagcatctg acaataccta tatttcgatt gaagacaaag aagcggtaga tttccgtgaa tctattgttg agttcttaat gaccaaccac ccacacgact gtccagtgtg tgaagagggt ggtcactgtc atttacaaga tatgactgta atgacaggtc acgaccgtcg tcgctatcgt tttactaagc gtacacatta caaccaagaa ctaggctcat tcattgcaca tgaaatgaac cgttgtattg cttgttatcg ttgtgttcgt tactacaagg attatgcagg cggtacagac tttggtgtat atgccaatgc atcacgtgtt tactttggtc gtccagaatc aggtacttta gagtctgaat tctctggtaa cttgactgaa gtatgtccaa ctggtgtatt taccgataag actcattcag agcgctataa ccgtaaatgg gatatgcaat atgcaccaag cgtgtgtcat ggctgttctt caggttgtaa catctctccg ggtgagcgtt atggcgaaat ccgtcgtgta gaaaaccgtt tcaatggttc ggttaaccaa tacttccttt gtgacaaagg ccgttttggc acaggttatg taaaccgtgc agatcgtcca cgtcaacctc agttccgtaa tggcgaaaca gtagctactg tttctgttga ccaagcgttg gaccaaacaa ttgcgaaact tgaaggtaaa aaagttctag gtatcggttc gccacgtgca agcttagaat caaattacgc attgcgtgaa ctagtgggcc aagcaaacta ctcaacaggt gttgcacaaa aagagcaaag cctcgttgaa cttgctgctt caatcatgca aacagagggt atttacaacc caaacatgcg tgaaattgaa agctacgatg cagtattaat tttgggtgaa gatttaactc aaactgctcc acgtatggca ttgtcagttc gtcaagctgc aaaaaataaa gctcgcgaaa tggctgcaaa aacacgtaca ccggactggt tggctgagcc tgtacaacgt attggtcagg aagcgaaatc tccgatctat atcttggctg caactcaaac tcgtcttgca gatgttgcga caggtgaagt tgttgcatct ccaaatgata ttgcgcgttt aggttttgca gttgctgcgg cagttaaagg tgaaacagtt aatggtttag atgaagatgc aaaagcattt gctcaaacta ttgccgacac tttaaaagca gcaaacaaac cgttaattat tgcgggtacg agcttgcaag atgcttcaat tatggaagcg gcagcagagt taacgcaaaa ccttggctca aaagctggtt taagccttgt tgttcctgaa gtgaactcta tgggcttggc attatttggt ggcttaagcc ttgaacaagc atttgctcaa gactacgaca cacttgttat tgttgaaaat gacttattcc gtcgtttacc ggccgctcaa gttaaagcag cacttgataa agctgaaaca gtaatcgtgc ttgaccacag tgaaactgaa actgtgaaac aggctgatat tgtactttca gcagcaagct ttgctgaagg tgatggtact gtcgtaagcc aagaaggtcg tgcacaacgc ttctaccaag tttacgatcc aagctactac aaacctgaat acgcgattaa agaatcttgg cgttggttgc atgccattga aactggcctt aaaggcaagc caattgattg gacagtactt gacgacgtaa tcgaaacaat cgtgaagaac gttccagtac ttgaagcaat tcaagatgtt gcgccagacg ctggctaccg tgtacatggt ttgaaaattg ctcgtgaacc acgtcgttat tctggtcgta cagcaatgcg tgcgccttta tcggttcatg aacctaaaca accgacagat ccagactcag cattaacatt ctctatggaa ggttatgtag gtccgcaaaa agcttcttcg cttgtaccat ttgcgtgggc accaggttgg aactctccac aatcttggaa caaataccaa gatgaagtgg gtggtcactt aaaaggtggt gattcaggcg ttcgtttatt cgaccgttta gcaaaacgtc cagcacgtag ctatgtcgct ccaacagctg tagttgcaaa tcctgaaagc ttccgtcttg taccaatgca ccatatcttt ggttctggcg aattcacaat taaaacacct gcaatggaaa ctcgtattcc tgaagctgta tttgctgtgg gcgaacaaga cgcaacacgt ttgaatctac aagaaggcca acgtattaca gtaaaagcag gcgaaacaac tgtgagctta cctgtacaag taattgaata tttacctaca ggttatattg gttacccagt tggtttagct ccgacagtat ctttagctga acctgtttca gtggcattag gagtgtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat