AcbaC.18465.a

3-deoxy-D-manno-2-octulosonate transferase

CENTER ID: AcbaC.18465.a
ORGANISM: Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AcbaC.18465.a.B1.GE38878 full length 1 430
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|470.1345.peg.166
GenBank: AKA29966.1
PGAT: acinetobacter:ABUW_0171
RefSeq: WP_000224849.1
UniProt: A0A0D5YDZ3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
LATPFWYNTA LHLLKPFYRW RIKRRAESLE LYQQECLERF GPFEAPKNVK AIWFHAVSVG ETNAAQPLIE YYLKLGQPVL VTNTTKTGQA RAKSLFLKEP YLDLFQAVYL PVDQKPLLKK FFELYQPKLL ALVETELWPN LIDQAKLQHV PCLLLNARLS EKSAKGYGKV SGLTAGMLKQ LDWVLAQDSA TRQRYVELGL DEHKSQVVGN IKFDIHAPEA FIKQAAQLRQ QWYLENRQVV TIASTHAPEE QQILEALAPY LNSDRELVCI VVPRHPERFD EVFEICQNLN LITHRRSMGQ SIHASTQVYL ADSMGELWLW YALSQVCFVG GSLNEPGGGH NILEPMVLNV PTVVGPRYFN FQTIVDEFID ENAVLIAQDA QQVVDIWLAC LAEPEATEQL VVQAHKVLQR NQGSLQKHIG VINRYLAEKS
NT Sequence
TTGGCGACCC CATTTTGGTA CAACACCGCA CTTCATTTAT TAAAACCGTT TTATCGTTGG CGGATAAAAA GACGTGCAGA AAGTCTAGAA TTATATCAGC AGGAATGTCT GGAAAGATTT GGGCCGTTTG AAGCACCGAA GAATGTAAAA GCGATCTGGT TTCATGCTGT TTCAGTCGGG GAAACCAATG CTGCACAGCC TTTAATTGAA TATTACCTAA AACTTGGGCA GCCAGTCTTA GTGACCAATA CCACGAAAAC AGGTCAGGCT CGTGCCAAGT CACTTTTTCT AAAAGAACCT TATTTAGATT TATTTCAAGC CGTTTATTTG CCTGTAGACC AGAAGCCTCT TTTAAAAAAA TTTTTTGAGT TATATCAGCC AAAGCTTTTA GCACTGGTTG AAACTGAACT TTGGCCAAAT TTAATCGATC AAGCCAAGTT ACAGCATGTA CCTTGTTTGC TGCTTAATGC TCGGTTGTCA GAAAAATCTG CAAAAGGATA TGGCAAAGTC TCGGGTTTAA CCGCAGGTAT GTTAAAACAG CTGGACTGGG TGTTAGCGCA AGATAGTGCA ACTCGTCAGC GTTATGTTGA GCTTGGTTTA GACGAACACA AAAGTCAGGT CGTTGGTAAT ATTAAATTTG ATATTCATGC GCCAGAGGCT TTTATTAAAC AAGCAGCGCA ATTGCGTCAG CAGTGGTATC TGGAAAATCG GCAGGTTGTG ACAATTGCCA GTACACATGC ACCCGAAGAA CAACAGATTT TGGAAGCACT CGCACCTTAT TTAAATTCAG ATCGTGAGTT GGTGTGTATT GTGGTGCCTC GTCATCCTGA GCGTTTCGAT GAAGTATTCG AAATTTGCCA AAATTTAAAT TTAATTACGC ATCGTAGAAG TATGGGCCAA AGTATTCATG CCAGCACGCA AGTTTATCTC GCTGACAGTA TGGGTGAGCT CTGGTTATGG TATGCCTTAA GTCAGGTGTG TTTTGTAGGC GGTTCTTTAA ATGAGCCGGG TGGGGGGCAT AATATTTTAG AACCTATGGT TTTAAATGTA CCTACTGTAG TTGGACCGCG TTATTTTAAC TTTCAAACGA TTGTCGATGA GTTCATTGAT GAAAATGCTG TGCTTATTGC TCAAGATGCA CAGCAGGTCG TTGATATCTG GTTAGCATGT CTGGCAGAAC CTGAGGCGAC TGAACAGTTA GTGGTACAGG CGCATAAAGT GTTGCAACGT AATCAAGGTT CCTTACAAAA ACATATCGGG GTGATTAATC GCTATCTGGC CGAAAAATCA TGA
Details for AcbaC.18465.a.B1.GE38878
HARVESTED ON: 1/29/2015
SEQUENCED ON: 1/15/2015
EXPECTED MW: 50kDa
OBSERVED MW: 46kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHML ATPFWYNTAL HLLKPFYRWR IKRRAESLEL YQQECLERFG PFEAPKNVKA IWFHAVSVGE TNAAQPLIEY YLKLGQPVLV TNTTKTGQAR AKSLFLKEPY LDLFQAVYLP VDQKPLLKKF FELYQPKLLA LVETELWPNL IDQAKLQHVP CLLLNARLSE KSAKGYGKVS GLTAGMLKQL DWVLAQDSAT RQRYVELGLD EHKSQVVGNI KFDIHAPEAF IKQAAQLRQQ WYLENRQVVT IASTHAPEEQ QILEALAPYL NSDRELVCIV VPRHPERFDE VFEICQNLNL ITHRRSMGQS IHASTQVYLA DSMGELWLWY ALSQVCFVGG SLNEPGGGHN ILEPMVLNVP TVVGPRYFNF QTIVDEFIDE NAVLIAQDAQ QVVDIWLACL AEPEATEQLV VQAHKVLQRN QGSLQKHIGV I
Validated NT Sequence
ttaatcaccc cgatatgttt ttgtaaggaa ccttgattac gttgcaacac tttatgcgcc tgtaccacta actgttcagt cgcctcaggt tctgccagac atgctaacca gatatcaacg acctgctgtg catcttgagc aataagcaca gcattttcat caatgaactc atcgacaatc gtttgaaagt taaaataacg cggtccaact acagtaggta catttaaaac cataggttct aaaatattat gccccccacc cggctcattt aaagaaccgc ctacaaaaca cacctgactt aaggcatacc ataaccagag ctcacccata ctgtcagcga gataaacttg cgtgctggca tgaatacttt ggcccatact tctacgatgc gtaattaaat ttaaattttg gcaaatttcg aatacttcat cgaaacgctc aggatgacga ggcaccacaa tacacaccaa ctcacgatct gaatttaaat aaggtgcgag tgcttccaaa atctgttgtt cttcgggtgc atgtgtactg gcaattgtca caacctgccg attttccaga taccactgct gacgcaattg cgctgcttgt ttaataaaag cctctggcgc atgaatatca aatttaatat taccaacgac ctgacttttg tgttcgtcta aaccaagctc aacataacgc tgacgagttg cactatcttg cgctaacacc cagtccagct gttttaacat acctgcggtt aaacccgaga ctttgccata tccttttgca gatttttctg acaaccgagc attaagcagc aaacaaggta catgctgtaa cttggcttga tcgattaaat ttggccaaag ttcagtttca accagtgcta aaagctttgg ctgatataac tcaaaaaatt tttttaaaag aggcttctgg tctacaggca aataaacggc ttgaaataaa tctaaataag gttcttttag aaaaagtgac ttggcacgag cctgacctgt tttcgtggta ttggtcacta agactggctg cccaagtttt aggtaatatt caattaaagg ctgtgcagca ttggtttccc cgactgaaac agcatgaaac cagatcgctt ttacattctt cggtgcttca aacggcccaa atctttccag acattcctgc tgatataatt ctagactttc tgcacgtctt tttatccgcc aacgataaaa cggttttaat aaatgaagtg cggtgttgta ccaaaatggg gtcgccaaca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHLA TPFWYNTALH LLKPFYRWRI KRRAESLELY QQECLERFGP FEAPKNVKAI WFHAVSVGET NAAQPLIEYY LKLGQPVLVT NTTKTGQARA KSLFLKEPYL DLFQAVYLPV DQKPLLKKFF ELYQPKLLAL VETELWPNLI DQAKLQHVPC LLLNARLSEK SAKGYGKVSG LTAGMLKQLD WVLAQDSATR QRYVELGLDE HKSQVVGNIK FDIHAPEAFI KQAAQLRQQW YLENRQVVTI ASTHAPEEQQ ILEALAPYLN SDRELVCIVV PRHPERFDEV FEICQNLNLI THRRSMGQSI HASTQVYLAD SMGELWLWYA LSQVCFVGGS LNEPGGGHNI LEPMVLNVPT VVGPRYFNFQ TIVDEFIDEN AVLIAQDAQQ VVDIWLACLA EPEATEQLVV QAHKVLQRNQ GSLQKHIGVI NRYLAEKS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttggcga ccccattttg gtacaacacc gcacttcatt tattaaaacc gttttatcgt tggcggataa aaagacgtgc agaaagtcta gaattatatc agcaggaatg tctggaaaga tttgggccgt ttgaagcacc gaagaatgta aaagcgatct ggtttcatgc tgtttcagtc ggggaaacca atgctgcaca gcctttaatt gaatattacc taaaacttgg gcagccagtc ttagtgacca ataccacgaa aacaggtcag gctcgtgcca agtcactttt tctaaaagaa ccttatttag atttatttca agccgtttat ttgcctgtag accagaagcc tcttttaaaa aaattttttg agttatatca gccaaagctt ttagcactgg ttgaaactga actttggcca aatttaatcg atcaagccaa gttacagcat gtaccttgtt tgctgcttaa tgctcggttg tcagaaaaat ctgcaaaagg atatggcaaa gtctcgggtt taaccgcagg tatgttaaaa cagctggact gggtgttagc gcaagatagt gcaactcgtc agcgttatgt tgagcttggt ttagacgaac acaaaagtca ggtcgttggt aatattaaat ttgatattca tgcgccagag gcttttatta aacaagcagc gcaattgcgt cagcagtggt atctggaaaa tcggcaggtt gtgacaattg ccagtacaca tgcacccgaa gaacaacaga ttttggaagc actcgcacct tatttaaatt cagatcgtga gttggtgtgt attgtggtgc ctcgtcatcc tgagcgtttc gatgaagtat tcgaaatttg ccaaaattta aatttaatta cgcatcgtag aagtatgggc caaagtattc atgccagcac gcaagtttat ctcgctgaca gtatgggtga gctctggtta tggtatgcct taagtcaggt gtgttttgta ggcggttctt taaatgagcc gggtgggggg cataatattt tagaacctat ggttttaaat gtacctactg tagttggacc gcgttatttt aactttcaaa cgattgtcga tgagttcatt gatgaaaatg ctgtgcttat tgctcaagat gcacagcagg tcgttgatat ctggttagca tgtctggcag aacctgaggc gactgaacag ttagtggtac aggcgcataa agtgttgcaa cgtaatcaag gttccttaca aaaacatatc ggggtgatta atcgctatct ggccgaaaaa tcatgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat