SSGCID: Target Page

AcbaC.18461.a

2-octaprenyl-6-methoxyphynol hydroxylase, FAD/NAD UbiH

CENTER ID:	AcbaC.18461.a
ORGANISM:	Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AcbaC.18461.a.B1.GE38877	full length		1	402

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|470.1345.peg.2809
GenBank:	AKA32570.1
PGAT:	acinetobacter:ABUW_2855
RefSeq:	WP_001187365.1
UniProt:	A0A0D5YJR3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MQQQVIIVGG GMVGLSLSLM LAKANIAVKL LEAVKYPNYD DQNVAPYHSS FDARNTALSR RSVQIYQKLG LWDALQQHAT PILQVHITEQ GSFGKARLIA EQEKVESFGQ VIENAWLGRV LLTQVRQQPL IELIDGVQVT ALTQNAEQVH IEAQRGDEVL KLESKLLIAA DGRDSFCRQA IGVGVDVHDY DQVAIVTTVQ TSKPHEHVGF ERFSALGPLA LLPLPGEYRR SVVWPVKKGT EGEWLGEEND QHFLDALQKT YGDRAGKFEK TGKRFSYPLS QVLAHKQAVG RVILMGNAAH TIHPVAGQGF NLCLRDADVL LRYLVNQLSA SDDIGNPDNL LAYEQARLSD QQRVIKFCDT VVRGFSNQNP LLKLIRNTGL IAFDVIPGVK PLVANYAMGL KA

NT Sequence

ATGCAACAAC AAGTGATTAT TGTAGGTGGG GGTATGGTGG GTTTAAGCCT TTCCCTCATG CTGGCGAAGG CTAATATCGC GGTAAAGCTA TTAGAAGCTG TGAAATACCC AAACTATGAT GATCAAAATG TTGCCCCTTA TCATTCCAGT TTTGATGCGC GGAATACGGC TTTATCACGT CGTAGCGTAC AGATTTATCA GAAGTTAGGG TTATGGGATG CCTTACAACA GCATGCCACT CCAATTTTGC AAGTTCACAT TACTGAACAA GGCAGTTTTG GAAAAGCACG TTTAATTGCA GAACAAGAGA AAGTCGAAAG CTTTGGGCAA GTCATTGAAA ATGCATGGCT CGGTCGTGTT TTATTAACTC AAGTACGTCA ACAGCCTTTG ATTGAACTGA TTGATGGCGT ACAAGTAACT GCTTTAACTC AAAATGCTGA ACAGGTACAT ATTGAAGCTC AGCGTGGTGA TGAAGTCTTA AAGCTTGAGT CAAAATTATT AATTGCAGCA GATGGGCGCG ATTCTTTCTG TCGTCAAGCA ATTGGTGTGG GTGTTGATGT TCACGACTAT GATCAGGTTG CGATCGTTAC GACTGTTCAG ACTTCAAAAC CGCATGAGCA TGTAGGGTTT GAACGTTTTA GTGCATTAGG GCCTTTAGCT TTGTTACCAT TACCGGGTGA ATACCGTCGC TCAGTGGTAT GGCCTGTTAA AAAAGGCACT GAAGGTGAGT GGTTGGGTGA AGAAAACGAC CAACACTTTT TAGATGCTTT GCAAAAAACT TATGGTGACA GAGCGGGTAA ATTTGAAAAA ACGGGTAAAC GCTTTAGTTA TCCGCTTTCA CAAGTGCTTG CGCATAAACA AGCTGTCGGA CGCGTTATTT TAATGGGTAA TGCAGCGCAT ACAATCCATC CTGTTGCTGG GCAAGGTTTT AATTTATGTC TGCGTGATGC AGATGTATTG TTGCGTTATT TAGTGAACCA GTTAAGCGCA TCTGATGATA TTGGTAACCC AGATAATTTG CTTGCCTATG AACAAGCCCG TTTATCTGAC CAGCAGCGAG TAATTAAATT CTGCGATACC GTAGTTCGGG GCTTTAGTAA CCAAAACCCA TTATTAAAAT TAATCCGTAA TACGGGATTA ATTGCATTTG ATGTCATTCC GGGTGTGAAG CCACTGGTAG CAAACTATGC AATGGGGTTA AAAGCATGA

Details for AcbaC.18461.a.B1.GE38877

HARVESTED ON:	1/29/2015
SEQUENCED ON:	1/15/2015
EXPECTED MW:	45kDa
OBSERVED MW:	45kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMQ QQVIIVGGGM VGLSLSLMLA KANIAVKLLE AVKYPNYDDQ NVAPYHSSFD ARNTALSRRS VQIYQKLGLW DALQQHATPI LQVHITEQGS FGKARLIAEQ EKVESFGQVI ENAWLGRVLL TQVRQQPLIE LIDGVQVTAL TQNAEQVHIE AQRGDEVLKL ESKLLIAADG RDSFCRQAIG VGVDVHDYDQ VAIVTTVQTS KPHEHVGFER FSALGPLALL PLPGEYRRSV VWPVKKGTEG EWLGEENDQH FLDALQKTYG DRAGKFEKTG KRFSYPLSQV LAHKQAVGRV ILMGNAAHTI HPVAGQGFNL CLRDADVLLR YLVNQLSASD DIGNPDNLLA YEQARLSDQQ RVIKFCDTVV RGFSNQNPLL KLIRNTGLIA FDVIPGVKPL VA

Validated NT Sequence

gtttgctacc agtggcttca cacccggaat gacatcaaat gcaattaatc ccgtattacg gattaatttt aataatgggt tttggttact aaagccccga actacggtat cgcagaattt aattactcgc tgctggtcag ataaacgggc ttgttcatag gcaagcaaat tatctgggtt accaatatca tcagatgcgc ttaactggtt cactaaataa cgcaacaata catctgcatc acgcagacat aaattaaaac cttgcccagc aacaggatgg attgtatgcg ctgcattacc cattaaaata acgcgtccga cagcttgttt atgcgcaagc acttgtgaaa gcggataact aaagcgttta cccgtttttt caaatttacc cgctctgtca ccataagttt tttgcaaagc atctaaaaag tgttggtcgt tttcttcacc caaccactca ccttcagtgc cttttttaac aggccatacc actgagcgac ggtattcacc cggtaatggt aacaaagcta aaggccctaa tgcactaaaa cgttcaaacc ctacatgctc atgcggtttt gaagtctgaa cagtcgtaac gatcgcaacc tgatcatagt cgtgaacatc aacacccaca ccaattgctt gacgacagaa agaatcgcgc ccatctgctg caattaataa ttttgactca agctttaaga cttcatcacc acgctgagct tcaatatgta cctgttcagc attttgagtt aaagcagtta cttgtacgcc atcaatcagt tcaatcaaag gctgttgacg tacttgagtt aataaaacac gaccgagcca tgcattttca atgacttgcc caaagctttc gactttctct tgttctgcaa ttaaacgtgc ttttccaaaa ctgccttgtt cagtaatgtg aacttgcaaa attggagtgg catgctgttg taaggcatcc cataacccta acttctgata aatctgtacg ctacgacgtg ataaagccgt attccgcgca tcaaaactgg aatgataagg ggcaacattt tgatcatcat agtttgggta tttcacagct tctaatagct ttaccgcgat attagccttc gccagcatga gggaaaggct taaacccacc atacccccac ctacaataat cacttgttgt tgcatatggt ggtggtggtg gtgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcaac aacaagtgat tattgtaggt gggggtatgg tgggtttaag cctttccctc atgctggcga aggctaatat cgcggtaaag ctattagaag ctgtgaaata cccaaactat gatgatcaaa atgttgcccc ttatcattcc agttttgatg cgcggaatac ggctttatca cgtcgtagcg tacagattta tcagaagtta gggttatggg atgccttaca acagcatgcc actccaattt tgcaagttca cattactgaa caaggcagtt ttggaaaagc acgtttaatt gcagaacaag agaaagtcga aagctttggg caagtcattg aaaatgcatg gctcggtcgt gttttattaa ctcaagtacg tcaacagcct ttgattgaac tgattgatgg cgtacaagta actgctttaa ctcaaaatgc tgaacaggta catattgaag ctcagcgtgg tgatgaagtc ttaaagcttg agtcaaaatt attaattgca gcagatgggc gcgattcttt ctgtcgtcaa gcaattggtg tgggtgttga tgttcacgac tatgatcagg ttgcgatcgt tacgactgtt cagacttcaa aaccgcatga gcatgtaggg tttgaacgtt ttagtgcatt agggccttta gctttgttac cattaccggg tgaataccgt cgctcagtgg tatggcctgt taaaaaaggc actgaaggtg agtggttggg tgaagaaaac gaccaacact ttttagatgc tttgcaaaaa acttatggtg acagagcggg taaatttgaa aaaacgggta aacgctttag ttatccgctt tcacaagtgc ttgcgcataa acaagctgtc ggacgcgtta ttttaatggg taatgcagcg catacaatcc atcctgttgc tgggcaaggt tttaatttat gtctgcgtga tgcagatgta ttgttgcgtt atttagtgaa ccagttaagc gcatctgatg atattggtaa cccagataat ttgcttgcct atgaacaagc ccgtttatct gaccagcagc gagtaattaa attctgcgat accgtagttc ggggctttag taaccaaaac ccattattaa aattaatccg taatacggga ttaattgcat ttgatgtcat tccgggtgtg aagccactgg tagcaaacta tgcaatgggg ttaaaagcat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat