SSGCID: Target Page

AcbaC.18222.a

glutaminyl-tRNA synthetase

CENTER ID:	AcbaC.18222.a
ORGANISM:	Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AcbaC.18222.a.B1.GE38876	full length		1	575

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AcbaC.18222.a.B1.PW37732	full length		1	575

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|470.1345.peg.1570
GenBank:	AKA31353.1
PGAT:	acinetobacter:ABUW_1615
RefSeq:	WP_000803936.1
UniProt:	A0A0D5YHH5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKPNDVVSNL PNNPTPNTHA SVDSAQQEQQ AGLDFVRQVI TDDLAAGRAK QIVTRFPPEP NGYLHIGHVK AICLNFGVAE EFNGLCNLRF DDTNPDAEEQ EYVDGIANDV KWLGFSWNGE PRYASGYFDQ LYAWAIQLIE QGDAYVDLQS PEEIKLNRGS FVEPGKNSPY RDASVEENLA RFEKMRNGEL KEGEAVLRAK IDMASPNVHM RDPILYRVLH SEHHQTGDKW KIYPMYDYAH PLSDAIEGIT HSLCTLEFQD HRPFYDWIVE KVKSKAVPHQ YESSRLNVDY TITSKRKLRK LVEGGYVNGW DDPRMPTVVG MRRRGFTPEG LRDFCKRVGV SKTDGIVDVA MLEFCIRQSL ENTAARGMAV LNPLKVTLTN LPEDLDLTHA RHPNVDMGER VIPLTKEIYI DRKDFEEVPP KGFKRLIPDG EVRLRHAYVI KCDEVIKDAN GEVIELKCSI DPDTLGKNPE GRKVKGVIHW VSATKGIPAE VRIYDRLFTE ADPETGDDFL ANLNPDSLKV VQAVIEPALA QAKPEDRFQF EREGYFVADQ YDHTPEKPVF NRVLDLKDSF KPEKK

NT Sequence

ATGAAGCCTA ATGATGTTGT CTCGAACCTG CCAAACAACC CGACACCGAA TACTCATGCC TCTGTCGATT CTGCGCAGCA AGAACAACAG GCTGGATTAG ATTTTGTTCG CCAAGTCATT ACCGATGATT TAGCTGCCGG ACGTGCCAAA CAAATCGTAA CGCGTTTCCC GCCAGAACCA AATGGTTATT TACATATTGG TCACGTAAAA GCAATTTGCC TCAACTTTGG CGTTGCTGAA GAGTTTAATG GTCTTTGTAA TTTACGTTTT GACGATACTA ACCCGGATGC GGAAGAACAA GAGTATGTTG ATGGTATCGC CAATGATGTG AAATGGCTTG GATTTAGCTG GAATGGCGAA CCACGTTATG CTTCCGGTTA TTTTGATCAG CTATATGCTT GGGCAATTCA GTTGATTGAA CAAGGCGATG CATATGTTGA TTTGCAGTCT CCAGAAGAAA TCAAATTAAA TCGTGGTAGC TTTGTTGAGC CAGGTAAAAA TTCGCCATAT CGTGATGCTT CTGTTGAAGA AAACCTTGCT CGTTTTGAAA AAATGCGCAA TGGTGAACTT AAAGAAGGTG AAGCTGTTTT ACGTGCCAAA ATTGATATGG CAAGCCCGAA TGTACATATG CGTGACCCAA TTTTGTATCG TGTTTTACAT TCAGAGCATC ACCAAACAGG TGATAAGTGG AAAATCTACC CAATGTATGA CTATGCTCAT CCATTGTCTG ATGCGATCGA AGGAATCACA CATTCACTTT GTACATTAGA GTTCCAAGAC CACCGTCCGT TTTATGACTG GATTGTTGAA AAAGTTAAGT CTAAAGCTGT GCCGCACCAA TATGAATCTT CTCGTTTAAA CGTAGATTAC ACCATTACGT CTAAACGTAA ATTACGTAAA TTGGTTGAAG GCGGTTATGT GAATGGATGG GATGATCCTC GTATGCCAAC GGTTGTCGGT ATGCGTCGCC GTGGTTTCAC ACCAGAAGGC CTACGTGATT TCTGTAAACG TGTAGGTGTG TCTAAAACTG ACGGTATTGT GGATGTCGCA ATGCTTGAGT TCTGTATTCG TCAATCTTTA GAGAATACGG CAGCGCGCGG TATGGCAGTG TTAAATCCAT TAAAAGTAAC ACTTACGAAC TTACCGGAAG ATTTAGACCT TACCCATGCT CGTCATCCTA ATGTCGATAT GGGTGAGCGC GTTATTCCAT TAACTAAAGA AATCTATATT GACCGTAAAG ATTTCGAAGA AGTACCGCCA AAAGGCTTTA AACGTTTAAT TCCTGATGGT GAAGTTCGTC TACGCCATGC ATATGTGATT AAGTGTGATG AAGTCATTAA AGATGCAAAC GGTGAAGTCA TTGAGCTGAA GTGTTCAATC GATCCGGATA CTTTAGGTAA AAATCCTGAA GGCCGCAAAG TAAAAGGCGT AATTCACTGG GTATCTGCAA CTAAAGGTAT TCCTGCTGAA GTACGTATTT ATGATCGACT GTTTACAGAA GCTGACCCTG AAACTGGTGA TGATTTCTTA GCAAACTTAA ATCCAGATTC TTTGAAAGTG GTTCAAGCTG TAATCGAACC TGCTTTAGCT CAAGCGAAGC CAGAAGATCG CTTCCAGTTT GAACGCGAAG GTTATTTCGT TGCTGATCAA TACGATCATA CTCCTGAGAA GCCTGTGTTT AACCGTGTTC TTGACTTAAA AGACAGCTTC AAGCCTGAGA AAAAGTAA

Details for AcbaC.18222.a.B1.GE38876

HARVESTED ON:	1/29/2015
SEQUENCED ON:	1/15/2015
EXPECTED MW:	66kDa
OBSERVED MW:	66kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMK PNDVVSNLPN NPTPNTHASV DSAQQEQQAG LDFVRQVITD DLAAGRAKQI VTRFPPEPNG YLHIGHVKAI CLNFGVAEEF NGLCNLRFDD TNPDAEEQEY VDGIANDVKW LGFSWNGEPR YASGYFDQLY AWAIQLIEQG DAYVDLQSPE EIKLNRGSFV EPGKNSPYRD ASVEENLARF EKMRNGELKE GEAVLRAKID MASPNVHMRD PILYRVLHSE HHQTGDKWKI YPMYDYAHPL SDAIEGITHS LCTLEFQDHR PFYDWIVEKV KSKAVPHQYE SSRLNVDYTI TSKRKLRKLV EGGYVNGWDD PRMPTVVGMR RRGFTPEGLR DFCKRVGVSK TDGIVDVAML EFCIRQSLEN TAARGMAVLN PLKVTLTNLP EDLDLTHARH PNVDMGERVI PLTKEIYIDR KDFEEVPPKG FKRLIPDGEV RLRHAYVIKC DEVIKDANGE VIELKCSIDP DTLGKNPEGR KVKGVIHWVS ATKGIPAEVR IYDRLFTEAD PETGDDFLAN LNPDSLKVVQ AVIEPALAQA KPEDRFQFER EGYFVADQYD HTPEKPVFNR VLDLKDSFKP EKK

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaagc ctaatgatgt tgtctcgaac ctgccaaaca acccgacacc gaatactcat gcctctgtcg attctgcgca gcaagaacaa caggctggat tagattttgt tcgccaagtc attaccgatg atttagctgc cggacgtgcc aaacaaatcg taacgcgttt cccgccagaa ccaaatggtt atttacatat tggtcacgta aaagcaattt gcctcaactt tggcgttgct gaagagttta atggtctttg taatttacgt tttgacgata ctaacccgga tgcggaagaa caagagtatg ttgatggtat cgccaatgat gtgaaatggc ttggatttag ctggaatggc gaaccacgtt atgcttccgg ttattttgat cagctatatg cttgggcaat tcagttgatt gaacaaggcg atgcatatgt tgatttgcag tctccagaag aaatcaaatt aaatcgtggt agctttgttg agccaggtaa aaattcgcca tatcgtgatg cttctgttga agaaaacctt gctcgttttg aaaaaatgcg caatggtgaa cttaaagaag gtgaagctgt tttacgtgcc aaaattgata tggcaagccc gaatgtacat atgcgtgacc caattttgta tcgtgtttta cattcagagc atcaccaaac aggtgataag tggaaaatct acccaatgta tgactatgct catccattgt ctgatgcgat cgaaggaatc acacattcac tttgtacatt agagttccaa gaccaccgtc cgttttatga ctggattgtt gaaaaagtta agtctaaagc tgtgccgcac caatatgaat cttctcgttt aaacgtagat tacaccatta cgtctaaacg taaattacgt aaattggttg aaggcggtta tgtgaatgga tgggatgatc ctcgtatgcc aacggttgtc ggtatgcgtc gccgtggttt cacaccagaa ggcctacgtg atttctgtaa acgtgtaggt gtgtctaaaa ctgacggtat tgtggatgtc gcaatgcttg agttctgtat tcgtcaatct ttagagaata cggcagcgcg cggtatggca gtgttaaatc cattaaaagt aacacttacg aacttaccgg aagatttaga ccttacccat gctcgtcatc ctaatgtcga tatgggtgag cgcgttattc cattaactaa agaaatctat attgaccgta aagatttcga agaagtaccg ccaaaaggct ttaaacgttt aattcctgat ggtgaagttc gtctacgcca tgcatatgtg attaagtgtg atgaagtcat taaagatgca aacggtgaag tcattgagct gaagtgttca atcgatccgg atactttagg taaaaatcct gaaggccgca aagtaaaagg cgtaattcac tgggtatctg caactaaagg tattcctgct gaagtacgta tttatgatcg actgtttaca gaagctgacc ctgaaactgg tgatgatttc ttagcaaact taaatccaga ttctttgaaa gtggttcaag ctgtaatcga acctgcttta gctcaagcga agccagaaga tcgcttccag tttgaacgcg aaggttattt cgttgctgat caatacgatc atactcctga gaagcctgtg tttaaccgtg ttcttgactt aaaagacagc ttcaagcctg agaaaaagtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat

Details for AcbaC.18222.a.B1.PW37732

PURIFICATION DATe:	6/16/2015
CONCENTRATION:	37mg/ml
OBSERVED MW:	67kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	12
VIAL VOLUME:	200µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAAGC CTAATGATGT TGTCTCGAAC CTGCCAAACA ACCCGACACC GAATACTCAT GCCTCTGTCG ATTCTGCGCA GCAAGAACAA CAGGCTGGAT TAGATTTTGT TCGCCAAGTC ATTACCGATG ATTTAGCTGC CGGACGTGCC AAACAAATCG TAACGCGTTT CCCGCCAGAA CCAAATGGTT ATTTACATAT TGGTCACGTA AAAGCAATTT GCCTCAACTT TGGCGTTGCT GAAGAGTTTA ATGGTCTTTG TAATTTACGT TTTGACGATA CTAACCCGGA TGCGGAAGAA CAAGAGTATG TTGATGGTAT CGCCAATGAT GTGAAATGGC TTGGATTTAG CTGGAATGGC GAACCACGTT ATGCTTCCGG TTATTTTGAT CAGCTATATG CTTGGGCAAT TCAGTTGATT GAACAAGGCG ATGCATATGT TGATTTGCAG TCTCCAGAAG AAATCAAATT AAATCGTGGT AGCTTTGTTG AGCCAGGTAA AAATTCGCCA TATCGTGATG CTTCTGTTGA AGAAAACCTT GCTCGTTTTG AAAAAATGCG CAATGGTGAA CTTAAAGAAG GTGAAGCTGT TTTACGTGCC AAAATTGATA TGGCAAGCCC GAATGTACAT ATGCGTGACC CAATTTTGTA TCGTGTTTTA CATTCAGAGC ATCACCAAAC AGGTGATAAG TGGAAAATCT ACCCAATGTA TGACTATGCT CATCCATTGT CTGATGCGAT CGAAGGAATC ACACATTCAC TTTGTACATT AGAGTTCCAA GACCACCGTC CGTTTTATGA CTGGATTGTT GAAAAAGTTA AGTCTAAAGC TGTGCCGCAC CAATATGAAT CTTCTCGTTT AAACGTAGAT TACACCATTA CGTCTAAACG TAAATTACGT AAATTGGTTG AAGGCGGTTA TGTGAATGGA TGGGATGATC CTCGTATGCC AACGGTTGTC GGTATGCGTC GCCGTGGTTT CACACCAGAA GGCCTACGTG ATTTCTGTAA ACGTGTAGGT GTGTCTAAAA CTGACGGTAT TGTGGATGTC GCAATGCTTG AGTTCTGTAT TCGTCAATCT TTAGAGAATA CGGCAGCGCG CGGTATGGCA GTGTTAAATC CATTAAAAGT AACACTTACG AACTTACCGG AAGATTTAGA CCTTACCCAT GCTCGTCATC CTAATGTCGA TATGGGTGAG CGCGTTATTC CATTAACTAA AGAAATCTAT ATTGACCGTA AAGATTTCGA AGAAGTACCG CCAAAAGGCT TTAAACGTTT AATTCCTGAT GGTGAAGTTC GTCTACGCCA TGCATATGTG ATTAAGTGTG ATGAAGTCAT TAAAGATGCA AACGGTGAAG TCATTGAGCT GAAGTGTTCA ATCGATCCGG ATACTTTAGG TAAAAATCCT GAAGGCCGCA AAGTAAAAGG CGTAATTCAC TGGGTATCTG CAACTAAAGG TATTCCTGCT GAAGTACGTA TTTATGATCG ACTGTTTACA GAAGCTGACC CTGAAACTGG TGATGATTTC TTAGCAAACT TAAATCCAGA TTCTTTGAAA GTGGTTCAAG CTGTAATCGA ACCTGCTTTA GCTCAAGCGA AGCCAGAAGA TCGCTTCCAG TTTGAACGCG AAGGTTATTT CGTTGCTGAT CAATACGATC ATACTCCTGA GAAGCCTGTG TTTAACCGTG TTCTTGACTT AAAAGACAGC TTCAAGCCTG AGAAAAAGTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT