AcbaC.18123.a

acetyl-CoA carboxylase, biotin carboxylase

CENTER ID: AcbaC.18123.a
ORGANISM: Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AcbaC.18123.a.B2.GE40891 ordered domain 1 443
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|470.1345.peg.1652
GenBank: AKA31433.1
PGAT: acinetobacter:ABUW_1696
RefSeq: WP_000939648.1
UniProt: R4LDE1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLQKVLIANR GEIALRITRA CKTLGIKTVG IYSDADKDLM HLRFVDEAVC IGPGASSDSY LNIPAIITAA EITGADAIHP GYGFLSENAE FAEIVESSGF TFIGPRPEHI RLMGNKVSAI VAMKKAGVPT VPGCDHAVTI HNALAEAKEI GFPLIVKAAA GGGGRGMRIV ERVDTLLESV QAAQRDAEMW FGDDTVYMER FLQKPRHVEV QVLGDGNGHA IHLYDRDCSL QRRHQKVLEE APAPNLPEQA RADILQACVH ACQLMQYRGA GTFEFLFEDG EFFFIEMNTR VQVEHPVTEM VTGVDIIEQQ LRIAAGLGLE LQQEDIEVRG HAIECRINAE DPTTFLPSPG KIESFYAPGG AGIRLDSHIY PGYSIPPYYD SMIAKLIAHG KDRETSLARM RQALDEMILT GIKTNIPLHK DLILQDKNFC SQAMDIHYLE KHLLKQVEEE KKAETA
NT Sequence
ATGTTGCAAA AAGTTTTAAT TGCAAACCGT GGTGAAATCG CTTTACGAAT TACCCGGGCT TGCAAAACAT TAGGAATCAA AACTGTTGGG ATCTATTCAG ATGCCGATAA AGATTTGATG CATTTACGTT TCGTTGATGA AGCAGTATGT ATTGGTCCGG GTGCAAGTAG TGACAGCTAT TTAAACATCC CGGCAATTAT TACTGCTGCA GAAATTACTG GTGCCGATGC AATTCATCCA GGTTATGGCT TCCTTTCGGA AAATGCCGAG TTTGCTGAAA TTGTAGAAAG CTCAGGTTTC ACATTTATTG GCCCTCGCCC AGAACATATT CGTTTAATGG GTAACAAGGT TTCTGCAATT GTTGCAATGA AAAAAGCTGG CGTACCTACG GTACCTGGCT GTGACCATGC TGTAACTATC CACAATGCCT TGGCTGAAGC GAAAGAAATC GGTTTCCCGC TTATTGTTAA AGCGGCAGCT GGTGGCGGTG GTCGTGGTAT GCGTATTGTT GAACGTGTAG ATACACTACT TGAGTCAGTT CAAGCGGCTC AACGTGATGC AGAAATGTGG TTCGGCGATG ATACCGTATA CATGGAACGC TTCTTACAAA AACCTCGCCA TGTCGAAGTT CAAGTTTTGG GTGATGGTAA TGGCCACGCG ATTCATTTAT ATGACCGTGA CTGTTCATTA CAACGTCGTC ACCAAAAAGT GTTAGAAGAA GCTCCTGCTC CAAATCTACC GGAACAAGCA CGTGCTGATA TTTTACAAGC CTGTGTCCAC GCATGTCAGT TAATGCAATA TCGTGGTGCT GGTACGTTTG AATTCTTATT TGAAGATGGT GAATTCTTCT TCATTGAAAT GAATACTCGT GTTCAGGTTG AGCACCCTGT AACTGAAATG GTAACTGGGG TTGACATTAT TGAACAGCAA TTACGTATTG CAGCTGGTTT AGGCTTAGAG CTTCAACAAG AAGATATTGA AGTTCGTGGT CATGCCATTG AATGTCGTAT CAATGCCGAA GATCCAACAA CATTCTTACC TTCACCAGGT AAAATTGAAA GTTTCTATGC ACCAGGCGGT GCGGGTATTC GTTTAGATTC TCATATCTAT CCTGGATATA GCATTCCGCC ATATTACGAT TCAATGATTG CAAAATTGAT TGCTCATGGT AAAGATCGTG AAACTTCACT TGCCCGCATG CGTCAAGCGT TAGATGAAAT GATCCTTACA GGAATTAAAA CCAATATTCC TTTACATAAA GATCTTATTT TGCAGGATAA AAACTTCTGT TCTCAAGCAA TGGACATCCA TTACCTTGAA AAACACTTGT TAAAGCAAGT GGAAGAAGAA AAGAAAGCTG AAACTGCTTA A
Details for AcbaC.18123.a.B2.GE40891
HARVESTED ON: 12/20/2016
SEQUENCED ON: 12/27/2016
EXPECTED MW: 50kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHHM LQKVLIANRG EIALRITRAC KTLGIKTVGI YSDADKDLMH LRFVDEAVCI GPGASSDSYL NIPAIITAAE ITGADAIHPG YGFLSENAEF AEIVESSGFT FIGPRPEHIR LMGNKVSAIV AMKKAGVPTV PGCDHAVTIH NALAEAKEIG FPLIVKAAAG GGGRGMRIVE RVDTLLESVQ AAQRDAEMWF GDDTVYMERF LQKPRHVEVQ VLGDGNGHAI HLYDRDCSLQ RRHQKVLEEA PAPNLPEQAR ADILQACVHA CQLMQYRGAG TFEFLFEDGE FFFIEMNTRV QVEHPVTEMV TGVDIIEQQL RIAAGLGLEL QQEDIEVRGH AIECRINAED PTTFLPSPGK IESFYAPGGA GIRLDSHIYP GYSIPPYYDS MIAKLIAHGK DRETSLARMR QALDEMILTG IKTNIPLHKD LILQDKNFCS QAMD
Validated NT Sequence
tgtccattgc ttgagaacag aagtttttat cctgcaaaat aagatcttta tgtaaaggaa tattggtttt aattcctgta aggatcattt catctaacgc ttgacgcatg cgggcaagtg aagtttcacg atctttacca tgagcaatca attttgcaat cattgaatcg taatatggcg gaatgctata tccaggatag atatgagaat ctaaacgaat acccgcaccg cctggtgcat agaaactttc aattttacct ggtgaaggta agaatgttgt tggatcttcg gcattgatac gacattcaat ggcatgacca cgaacttcaa tatcttcttg ttgaagctct aagcctaaac cagctgcaat acgtaattgc tgttcaataa tgtcaacccc agttaccatt tcagttacag ggtgctcaac ctgaacacga gtattcattt caatgaagaa gaattcacca tcttcaaata agaattcaaa cgtaccagca ccacgatatt gcattaactg acatgcgtgg acacaggctt gtaaaatatc agcacgtgct tgttccggta gatttggagc aggagcttct tctaacactt tttggtgacg acgttgtaat gaacagtcac ggtcatataa atgaatcgcg tggccattac catcacccaa aacttgaact tcgacatggc gaggtttttg taagaagcgt tccatgtata cggtatcatc gccgaaccac atttctgcat cacgttgagc cgcttgaact gactcaagta gtgtatctac acgttcaaca atacgcatac cacgaccacc gccaccagct gccgctttaa caataagcgg gaaaccgatt tctttcgctt cagccaaggc attgtggata gttacagcat ggtcacagcc aggtaccgta ggtacgccag cttttttcat tgcaacaatt gcagaaacct tgttacccat taaacgaata tgttctgggc gagggccaat aaatgtgaaa cctgagcttt ctacaatttc agcaaactcg gcattttccg aaaggaagcc ataacctgga tgaattgcat cggcaccagt aatttctgca gcagtaataa ttgccgggat gtttaaatag ctgtcactac ttgcacccgg accaatacat actgcttcat caacgaaacg taaatgcatc aaatctttat cggcatctga atagatccca acagttttga ttcctaatgt tttgcaagcc cgggtaattc gtaaagcgat ttcaccacgg tttgcaatta aaactttttg caacatatgg tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHML QKVLIANRGE IALRITRACK TLGIKTVGIY SDADKDLMHL RFVDEAVCIG PGASSDSYLN IPAIITAAEI TGADAIHPGY GFLSENAEFA EIVESSGFTF IGPRPEHIRL MGNKVSAIVA MKKAGVPTVP GCDHAVTIHN ALAEAKEIGF PLIVKAAAGG GGRGMRIVER VDTLLESVQA AQRDAEMWFG DDTVYMERFL QKPRHVEVQV LGDGNGHAIH LYDRDCSLQR RHQKVLEEAP APNLPEQARA DILQACVHAC QLMQYRGAGT FEFLFEDGEF FFIEMNTRVQ VEHPVTEMVT GVDIIEQQLR IAAGLGLELQ QEDIEVRGHA IECRINAEDP TTFLPSPGKI ESFYAPGGAG IRLDSHIYPG YSIPPYYDSM IAKLIAHGKD RETSLARMRQ ALDEMILTGI KTNIPLHKDL ILQDKNFCSQ AMDIHYLEKH L
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttgc aaaaagtttt aattgcaaac cgtggtgaaa tcgctttacg aattacccgg gcttgcaaaa cattaggaat caaaactgtt gggatctatt cagatgccga taaagatttg atgcatttac gtttcgttga tgaagcagta tgtattggtc cgggtgcaag tagtgacagc tatttaaaca tcccggcaat tattactgct gcagaaatta ctggtgccga tgcaattcat ccaggttatg gcttcctttc ggaaaatgcc gagtttgctg aaattgtaga aagctcaggt ttcacattta ttggccctcg cccagaacat attcgtttaa tgggtaacaa ggtttctgca attgttgcaa tgaaaaaagc tggcgtacct acggtacctg gctgtgacca tgctgtaact atccacaatg ccttggctga agcgaaagaa atcggtttcc cgcttattgt taaagcggca gctggtggcg gtggtcgtgg tatgcgtatt gttgaacgtg tagatacact acttgagtca gttcaagcgg ctcaacgtga tgcagaaatg tggttcggcg atgataccgt atacatggaa cgcttcttac aaaaacctcg ccatgtcgaa gttcaagttt tgggtgatgg taatggccac gcgattcatt tatatgaccg tgactgttca ttacaacgtc gtcaccaaaa agtgttagaa gaagctcctg ctccaaatct accggaacaa gcacgtgctg atattttaca agcctgtgtc cacgcatgtc agttaatgca atatcgtggt gctggtacgt ttgaattctt atttgaagat ggtgaattct tcttcattga aatgaatact cgtgttcagg ttgagcaccc tgtaactgaa atggtaactg gggttgacat tattgaacag caattacgta ttgcagctgg tttaggctta gagcttcaac aagaagatat tgaagttcgt ggtcatgcca ttgaatgtcg tatcaatgcc gaagatccaa caacattctt accttcacca ggtaaaattg aaagtttcta tgcaccaggc ggtgcgggta ttcgtttaga ttctcatatc tatcctggat atagcattcc gccatattac gattcaatga ttgcaaaatt gattgctcat ggtaaagatc gtgaaacttc acttgcccgc atgcgtcaag cgttagatga aatgatcctt acaggaatta aaaccaatat tcctttacat aaagatctta ttttgcagga taaaaacttc tgttctcaag caatggacat ccattacctt gaaaaacact tgtgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat