AcbaC.18055.a

DNA-directed RNA polymerase, beta subunit

CENTER ID: AcbaC.18055.a
ORGANISM: Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AcbaC.18055.a.B1.GE38873 full length 1 1362
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|470.1345.peg.3522
GenBank: AKA33281.1
PGAT: acinetobacter:ABUW_3590
RefSeq: WP_000331899.1
UniProt: Q20HS3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAYSYTEKKR IRKNFGKLPQ VMDAPYLLSI QVDSYRTFLQ DGKSPKNRED IGLQAAFRSV FPIESYSGNA ALEFVEYSLG KPEFDVRECI LRGSTYAAPM RVKIRLIIKD RETKSIKDVR EQEVYMGEIP LMTENGTFVI NGTERVIVSQ LHRSPGVFFD HDKGKTHSSG KVLYSARIIP YRGSWLDFEF DAKDLVYVRI DRRRKLLATV VLRALGYNNE QILNLFYEKV PVYLDMGSYQ IDLVPERLRG EMAQFDITDN EGKVIVEQGK RINARHVRQM EAAGLTKLSV PDEYLYERIT AEDITLRDGE VIAANTLLSH EVMVKLAEGG VKQFNILFTN DIDRGSFVAD TLRADLTRDR EEALVEIYKV MRPGEPPTKE AAENLFNNLF FSSERYDLSP VGRMKFNRRL GRPYEVGTDQ KSREVEGILS HEDIIDVLRT LVEIRNGKGE VDDIDHLGNR RVRSVGEMTE NQFRVGLVRV ERAVKERLSQ AETDNLSPQD LINAKPVAAA IKEFFGSSQL SQFMDQNNPL SEITHKRRVS ALGPGGLTRE RAGFEVRDVH QTHYGRVCPI ETPEGPNIGL INSLSVYAKA NDFGFLETPY RKVVDGRVTD DVEYLSAIEE VGTVIAQADS AVDKDGNLTE EFVSVRHQGE FVRMPPEKVT HMDVSAQQVV SVAASLIPFL EHDDANRALM GSNMQRQAVP TLRADKPLVG TGMEANVARD SGVCVIANRG GVIEYVDASR IVIRVNEDEM VAGEAGVDIY NLIKYTRSNQ NTCINQNVIV NLGDKVARGD ILADGPSTDM GELALGQNMR VAFMTWNGYN YEDSILLSER VLQEDRLTSI HIQELSCVAR DTKLGAEEIT ADIPNVGEAA LSKLDESGIV YIGAEVTAGD ILVGKVTPKG ETQLTPEEKL LRAIFGEKAA DVKDSSLRVP SGTKGTVIDV QVFTRDGLEK DDRALAIEKA QLDSYRKDLK EEYKIFEEAA RERVIRLLKG QESNGGGSTK RGDKLSEDLL SGLELVDLLE IQPADEAIAE RLTQIQVFLK EKSAEIDEKF AEKKRKLATG DELTTGVLKV VKVYLAVKRR IQPGDKMAGR HGNKGVVSNI LPVEDMPHDA NGVPVDIVLN PLGVPSRMNV GQILETHLGM AAKGLGDKIE KMLKEQRTVL ELREFLDKIY NKVGGEQEDL DSLTDEEILA LAGNLRAGVP LATPVFDGAE ESQIKDLLEL ADISRTGQTV LFDGRTGEQF DRPVTVGYMY MLKLNHLVDD KMHARSTGSY SLVTQQPLGG KAQFGGQRFG EMEVWALEAY GAAYTLQEML TVKSDDVEGR TRIYKNIVDG NHYMDPGMPE SFNVLTKEIR SLGINIELKN GD
NT Sequence
ATGGCATACT CATATACCGA AAAGAAACGG ATCCGTAAGA ATTTTGGTAA ATTGCCCCAA GTAATGGATG CACCGTACTT ATTATCGATT CAGGTCGATT CGTACAGAAC ATTCTTGCAA GATGGCAAAT CACCAAAAAA CCGCGAAGAT ATCGGTCTCC AAGCCGCATT TCGTTCAGTT TTTCCTATCG AAAGTTATTC TGGCAATGCT GCTTTAGAAT TTGTTGAGTA TAGCCTTGGT AAACCTGAGT TCGATGTACG CGAATGTATT CTTCGTGGCT CGACTTATGC GGCACCAATG CGCGTTAAAA TTCGTTTGAT TATTAAAGAT CGCGAAACTA AATCAATTAA AGACGTACGC GAACAAGAAG TCTATATGGG TGAAATTCCG CTCATGACTG AAAATGGTAC CTTTGTTATC AACGGTACTG AGCGTGTAAT CGTATCTCAA TTACACCGTT CGCCAGGCGT ATTCTTTGAC CATGATAAAG GTAAGACCCA CTCAAGTGGT AAAGTGTTGT ATTCAGCACG TATCATTCCT TACCGTGGTT CATGGTTAGA CTTCGAATTT GATGCAAAAG ACTTAGTTTA CGTACGTATC GACCGTCGTC GTAAATTACT TGCTACAGTT GTGTTACGTG CACTAGGTTA TAACAATGAA CAGATCTTGA ATTTGTTCTA TGAAAAAGTA CCTGTGTATC TTGACATGGG TAGCTATCAA ATTGACCTTG TACCTGAGCG TTTACGTGGT GAAATGGCTC AATTTGATAT TACTGACAAT GAAGGTAAAG TCATTGTTGA GCAAGGTAAA CGTATTAATG CTCGTCATGT ACGTCAAATG GAAGCTGCAG GTTTAACTAA GCTTTCAGTT CCTGATGAAT ACTTATATGA GCGTATCACT GCTGAAGATA TTACTTTACG TGATGGTGAA GTAATTGCTG CAAATACTCT GTTAAGCCAT GAAGTAATGG TGAAGTTGGC AGAAGGCGGT GTTAAGCAAT TTAATATCTT GTTCACTAAC GATATTGACC GTGGTTCATT CGTAGCTGAT ACATTACGTG CTGACTTGAC GCGTGATCGT GAAGAAGCAT TAGTAGAAAT CTACAAAGTA ATGCGTCCAG GCGAGCCACC AACAAAAGAA GCTGCTGAAA ACTTATTCAA TAACTTGTTC TTCTCTTCTG AACGCTATGA CTTATCTCCA GTAGGTCGTA TGAAGTTCAA CCGTCGTTTA GGTCGTCCTT ACGAAGTTGG TACTGATCAG AAGTCACGCG AAGTTGAAGG TATTTTATCG CACGAAGATA TTATCGATGT ATTACGTACA TTGGTTGAAA TCCGTAACGG TAAAGGTGAA GTCGACGATA TCGACCACTT AGGTAACCGT CGTGTACGTT CTGTTGGTGA AATGACAGAG AACCAATTCC GTGTAGGTTT AGTTCGTGTT GAGCGTGCTG TTAAAGAGCG TTTAAGCCAA GCAGAAACAG ATAACTTGTC TCCACAAGAT TTGATTAATG CAAAACCAGT TGCTGCTGCA ATCAAAGAAT TCTTTGGTTC AAGCCAGTTA TCTCAGTTTA TGGACCAAAA CAACCCACTA TCTGAGATTA CACATAAACG TCGTGTATCT GCGCTTGGTC CTGGTGGTTT AACACGTGAA CGTGCGGGCT TCGAAGTACG TGACGTACAC CAAACTCACT ATGGTCGTGT TTGTCCAATT GAAACTCCTG AAGGTCCAAA CATTGGTTTG ATCAACTCGC TTTCTGTATA CGCAAAAGCG AATGACTTCG GTTTCTTGGA AACTCCATAC CGCAAAGTTG TAGATGGTCG TGTAACTGAT GATGTTGAAT ATTTATCTGC AATTGAAGAA GTAGGCACTG TTATTGCACA GGCCGACTCT GCAGTAGATA AAGATGGCAA CTTAACAGAA GAATTCGTTT CTGTTCGTCA TCAAGGTGAA TTCGTACGTA TGCCGCCTGA AAAAGTAACG CATATGGACG TTTCTGCACA GCAGGTAGTA TCTGTTGCTG CATCACTTAT TCCATTCCTT GAACACGATG ACGCAAACCG TGCGCTCATG GGTTCAAACA TGCAACGTCA GGCAGTTCCT ACTTTACGTG CGGATAAACC GCTTGTAGGT ACAGGTATGG AAGCGAACGT TGCACGTGAC TCTGGTGTGT GTGTAATCGC AAACCGTGGC GGTGTAATTG AATATGTAGA TGCTTCTCGT ATCGTTATTC GTGTAAACGA AGATGAAATG GTTGCAGGTG AGGCAGGTGT AGATATCTAT AACCTCATCA AATATACGCG TTCAAACCAA AATACTTGTA TTAACCAAAA TGTTATCGTG AACTTGGGCG ACAAAGTTGC TCGTGGTGAC ATCTTGGCAG ACGGTCCGTC AACAGACATG GGTGAACTTG CGCTTGGTCA AAACATGCGT GTAGCGTTCA TGACATGGAA TGGTTACAAC TACGAAGACT CGATCTTGTT ATCTGAGCGT GTACTTCAAG AAGACCGTTT AACCTCTATT CACATTCAAG AATTGTCATG TGTAGCACGT GATACTAAGT TAGGTGCAGA AGAAATTACT GCCGATATTC CTAACGTAGG TGAAGCTGCG CTTTCTAAAC TTGATGAATC AGGTATCGTT TATATCGGTG CTGAAGTTAC TGCTGGTGAC ATCTTAGTTG GTAAAGTAAC GCCTAAAGGT GAAACTCAGT TAACTCCTGA AGAAAAACTG CTTCGTGCAA TTTTTGGTGA GAAAGCAGCT GACGTTAAAG ACTCATCTTT ACGTGTTCCA TCTGGTACTA AAGGTACAGT TATTGACGTT CAAGTCTTCA CTCGTGATGG CTTAGAGAAA GATGACCGTG CTTTAGCAAT TGAAAAAGCA CAGCTTGATT CTTATCGTAA AGACTTGAAA GAAGAATACA AGATCTTCGA AGAAGCGGCT CGTGAGCGTG TAATTCGTTT GCTTAAAGGC CAAGAGTCTA ATGGCGGTGG TTCAACTAAA CGTGGTGATA AACTTTCTGA AGATTTATTA TCTGGTTTAG AGCTTGTTGA CTTACTTGAA ATTCAACCAG CAGATGAAGC AATCGCTGAG CGTTTAACTC AAATTCAAGT GTTCTTGAAA GAGAAGAGCG CAGAAATCGA TGAGAAATTC GCTGAGAAGA AACGTAAGCT TGCAACAGGT GATGAATTAA CAACTGGTGT ATTGAAAGTT GTTAAAGTTT ACTTAGCTGT TAAACGTCGT ATTCAGCCTG GTGATAAGAT GGCTGGTCGT CACGGTAACA AAGGTGTTGT ATCTAACATC TTACCTGTTG AAGACATGCC ACATGATGCT AACGGTGTGC CGGTAGATAT CGTATTGAAC CCGCTGGGTG TACCATCTCG TATGAACGTG GGTCAGATTC TAGAGACTCA CTTGGGTATG GCGGCTAAAG GGCTTGGTGA CAAAATCGAA AAAATGTTGA AAGAACAACG TACAGTTTTA GAACTGCGCG AATTCTTAGA CAAGATTTAT AACAAAGTCG GCGGTGAGCA AGAAGATCTT GATAGCTTGA CTGATGAAGA AATTCTAGCG CTTGCAGGTA ACTTGCGTGC GGGTGTGCCT TTAGCTACTC CTGTATTTGA TGGTGCTGAA GAAAGTCAAA TTAAAGACTT ACTTGAATTG GCTGACATTT CACGTACGGG TCAAACAGTA TTGTTTGACG GACGTACAGG TGAACAGTTT GACCGTCCAG TAACTGTTGG TTACATGTAC ATGCTTAAAT TGAACCACTT GGTTGATGAC AAGATGCATG CGCGTTCAAC TGGTTCTTAC TCACTTGTTA CACAACAACC GCTTGGTGGT AAAGCACAAT TCGGTGGTCA GCGTTTCGGT GAGATGGAAG TGTGGGCACT TGAAGCATAC GGTGCAGCAT ATACACTCCA AGAAATGCTT ACAGTGAAGT CGGATGACGT TGAAGGCCGT ACTCGCATCT ATAAGAATAT TGTAGATGGT AACCATTATA TGGATCCGGG TATGCCTGAA TCGTTCAACG TATTGACCAA AGAGATCCGT TCTTTAGGTA TCAACATTGA ACTAAAAAAT GGTGACTAA
Details for AcbaC.18055.a.B1.GE38873
HARVESTED ON: 1/29/2015
SEQUENCED ON: 1/15/2015
EXPECTED MW: 153kDa
OBSERVED MW: 153kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMA YSYTEKKRIR KNFGKLPQVM DAPYLLSIQV DSYRTFLQDG KSPKNREDIG LQAAFRSVFP IESYSGNAAL EFVEYSLGKP EFDVRECILR GSTYAAPMRV KIRLIIKDRE TKSIKDVREQ EVYMGEIPLM TENGTFVING TERVIVSQLH RSPGVFFDHD KGKTHSSGKV LYSARIIPYR GSWLDFEFDA KDLVYVRIDR RRKLLATVVL RALGYNNEQI LNLFYEKVPV YLDMGSYQID LVPERLRGEM AQFDITDNEG KVIVEQGKRI NARHVRQMEA AGLTKLSVPD EYLYERITAE DITLRDGEVI AANTLLSHEV MVKLAEGGVK QFNILFTNDI DRGSFVADTL RADLTRDREE ALVEIYKVMR PGEPPTKEAA ENLFNNLFFS SERYDLSPVG RMKFNRRLGR PYEVGTDQKS REVEGILSHE DIIDVLRTLV EIRNGKGEVD DIDHLGNRRV RSVGEMTENQ FRVGLVRVER AVKERLSQAE TDNLSPQDLI NAKPVAAAIK EFFGSSQLSQ FMDQNNPLSE ITHKRRVSAL GPGGLTRERA GFEVRDVHQT HYGRVCPIET PEGPNIGLIN SLSVYAKAND FGFLETPYRK VVDGRVTDDV EYLSAIEEVG TVIAQADSAV DKDGNLTEEF VSVRHQGEFV RMPPEKVTHM DVSAQQVVSV AASLIPFLEH DDANRALMGS NMQRQAVPTL RADKPLVGTG MEANVARDSG VCVIANRGGV IEYVDASRIV IRVNEDEMVA GEAGVDIYNL IKYTRSNQNT CINQNVIVNL GDKVARGDIL ADGPSTDMGE LALGQNMRVA FMTWNGYNYE DSILLSERVL QEDRLTSIHI QELSCVARDT KLGAEEITAD IPNVGEAALS KLDESGIVYI GAEVTAGDIL VGKVTPKGET QLTPEEKLLR AIFGEKAADV KDSSLRVPSG TKGTVIDVQV FTRDGLEKDD RALAIEKAQL DSYRKDLKEE YKIFEEAARE RVIRLLKGQE SNGGGSTKRG DKLSEDLLSG LELVDLLEIQ PADEAIAERL TQIQVFLKEK SAEIDEKFAE KKRKLATGDE LTTGVLKVVK VYLAVKRRIQ PGDKMAGRHG NKGVVSNILP VEDMPHDANG VPVDIVLNPL GVPSRMNVGQ ILETHLGMAA KGLGDKIEKM LKEQRTVLEL REFLDKIYNK VGGEQEDLDS LTDEEILALA GNLRAGVPLA TPVFDGAEES QIKDLLELAD ISRTGQTVLF DGRTGEQFDR PVTVGYMYML KLNHLVDDKM HARSTGSYSL VTQQPLGGKA QFGGQRFGEM EVWALEAYGA AYTLQEMLTV KSDDVEGRTR IYKNIVDGNH YMDPGMPESF NVLTKEIRSL GINIELKNGD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcat actcatatac cgaaaagaaa cggatccgta agaattttgg taaattgccc caagtaatgg atgcaccgta cttattatcg attcaggtcg attcgtacag aacattcttg caagatggca aatcaccaaa aaaccgcgaa gatatcggtc tccaagccgc atttcgttca gtttttccta tcgaaagtta ttctggcaat gctgctttag aatttgttga gtatagcctt ggtaaacctg agttcgatgt acgcgaatgt attcttcgtg gctcgactta tgcggcacca atgcgcgtta aaattcgttt gattattaaa gatcgcgaaa ctaaatcaat taaagacgta cgcgaacaag aagtctatat gggtgaaatt ccgctcatga ctgaaaatgg tacctttgtt atcaacggta ctgagcgtgt aatcgtatct caattacacc gttcgccagg cgtattcttt gaccatgata aaggtaagac ccactcaagt ggtaaagtgt tgtattcagc acgtatcatt ccttaccgtg gttcatggtt agacttcgaa tttgatgcaa aagacttagt ttacgtacgt atcgaccgtc gtcgtaaatt acttgctaca gttgtgttac gtgcactagg ttataacaat gaacagatct tgaatttgtt ctatgaaaaa gtacctgtgt atcttgacat gggtagctat caaattgacc ttgtacctga gcgtttacgt ggtgaaatgg ctcaatttga tattactgac aatgaaggta aagtcattgt tgagcaaggt aaacgtatta atgctcgtca tgtacgtcaa atggaagctg caggtttaac taagctttca gttcctgatg aatacttata tgagcgtatc actgctgaag atattacttt acgtgatggt gaagtaattg ctgcaaatac tctgttaagc catgaagtaa tggtgaagtt ggcagaaggc ggtgttaagc aatttaatat cttgttcact aacgatattg accgtggttc attcgtagct gatacattac gtgctgactt gacgcgtgat cgtgaagaag cattagtaga aatctacaaa gtaatgcgtc caggcgagcc accaacaaaa gaagctgctg aaaacttatt caataacttg ttcttctctt ctgaacgcta tgacttatct ccagtaggtc gtatgaagtt caaccgtcgt ttaggtcgtc cttacgaagt tggtactgat cagaagtcac gcgaagttga aggtatttta tcgcacgaag atattatcga tgtattacgt acattggttg aaatccgtaa cggtaaaggt gaagtcgacg atatcgacca cttaggtaac cgtcgtgtac gttctgttgg tgaaatgaca gagaaccaat tccgtgtagg tttagttcgt gttgagcgtg ctgttaaaga gcgtttaagc caagcagaaa cagataactt gtctccacaa gatttgatta atgcaaaacc agttgctgct gcaatcaaag aattctttgg ttcaagccag ttatctcagt ttatggacca aaacaaccca ctatctgaga ttacacataa acgtcgtgta tctgcgcttg gtcctggtgg tttaacacgt gaacgtgcgg gcttcgaagt acgtgacgta caccaaactc actatggtcg tgtttgtcca attgaaactc ctgaaggtcc aaacattggt ttgatcaact cgctttctgt atacgcaaaa gcgaatgact tcggtttctt ggaaactcca taccgcaaag ttgtagatgg tcgtgtaact gatgatgttg aatatttatc tgcaattgaa gaagtaggca ctgttattgc acaggccgac tctgcagtag ataaagatgg caacttaaca gaagaattcg tttctgttcg tcatcaaggt gaattcgtac gtatgccgcc tgaaaaagta acgcatatgg acgtttctgc acagcaggta gtatctgttg ctgcatcact tattccattc cttgaacacg atgacgcaaa ccgtgcgctc atgggttcaa acatgcaacg tcaggcagtt cctactttac gtgcggataa accgcttgta ggtacaggta tggaagcgaa cgttgcacgt gactctggtg tgtgtgtaat cgcaaaccgt ggcggtgtaa ttgaatatgt agatgcttct cgtatcgtta ttcgtgtaaa cgaagatgaa atggttgcag gtgaggcagg tgtagatatc tataacctca tcaaatatac gcgttcaaac caaaatactt gtattaacca aaatgttatc gtgaacttgg gcgacaaagt tgctcgtggt gacatcttgg cagacggtcc gtcaacagac atgggtgaac ttgcgcttgg tcaaaacatg cgtgtagcgt tcatgacatg gaatggttac aactacgaag actcgatctt gttatctgag cgtgtacttc aagaagaccg tttaacctct attcacattc aagaattgtc atgtgtagca cgtgatacta agttaggtgc agaagaaatt actgccgata ttcctaacgt aggtgaagct gcgctttcta aacttgatga atcaggtatc gtttatatcg gtgctgaagt tactgctggt gacatcttag ttggtaaagt aacgcctaaa ggtgaaactc agttaactcc tgaagaaaaa ctgcttcgtg caatttttgg tgagaaagca gctgacgtta aagactcatc tttacgtgtt ccatctggta ctaaaggtac agttattgac gttcaagtct tcactcgtga tggcttagag aaagatgacc gtgctttagc aattgaaaaa gcacagcttg attcttatcg taaagacttg aaagaagaat acaagatctt cgaagaagcg gctcgtgagc gtgtaattcg tttgcttaaa ggccaagagt ctaatggcgg tggttcaact aaacgtggtg ataaactttc tgaagattta ttatctggtt tagagcttgt tgacttactt gaaattcaac cagcagatga agcaatcgct gagcgtttaa ctcaaattca agtgttcttg aaagagaaga gcgcagaaat cgatgagaaa ttcgctgaga agaaacgtaa gcttgcaaca ggtgatgaat taacaactgg tgtattgaaa gttgttaaag tttacttagc tgttaaacgt cgtattcagc ctggtgataa gatggctggt cgtcacggta acaaaggtgt tgtatctaac atcttacctg ttgaagacat gccacatgat gctaacggtg tgccggtaga tatcgtattg aacccgctgg gtgtaccatc tcgtatgaac gtgggtcaga ttctagagac tcacttgggt atggcggcta aagggcttgg tgacaaaatc gaaaaaatgt tgaaagaaca acgtacagtt ttagaactgc gcgaattctt agacaagatt tataacaaag tcggcggtga gcaagaagat cttgatagct tgactgatga agaaattcta gcgcttgcag gtaacttgcg tgcgggtgtg cctttagcta ctcctgtatt tgatggtgct gaagaaagtc aaattaaaga cttacttgaa ttggctgaca tttcacgtac gggtcaaaca gtattgtttg acggacgtac aggtgaacag tttgaccgtc cagtaactgt tggttacatg tacatgctta aattgaacca cttggttgat gacaagatgc atgcgcgttc aactggttct tactcacttg ttacacaaca accgcttggt ggtaaagcac aattcggtgg tcagcgtttc ggtgagatgg aagtgtgggc acttgaagca tacggtgcag catatacact ccaagaaatg cttacagtga agtcggatga cgttgaaggc cgtactcgca tctataagaa tattgtagat ggtaaccatt atatggatcc gggtatgcct gaatcgttca acgtattgac caaagagatc cgttctttag gtatcaacat tgaactaaaa aatggtgact gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat