AcbaC.18002.a
glutamine-dependent NAD+ synthetase
| CENTER ID: | AcbaC.18002.a |
| ORGANISM: | Acinetobacter baumannii AB5075-UW |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| AcbaC.18002.a.B1.GE38871 | full length | 1 | 541 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
5KHA
DEPOSITED: 6/14/2016
DETERMINATION: XRay
CLONE: AcbaC.18002.a.B1.GE38871
PROTEIN: AcbaC.18002.a.B1.PS02475
Publications by SSGCID
Toward a structome of Acinetobacter baumannii drug targets
Abendroth J, Barrett LK, Dranow DM, Edwards TE, Lorimer DD, Myler PJ, Phan I, Staker B, Subramanian S, Van Voorhis WC, Craig JC, Chun IKH, Barrett KF, Prentice RA, Tillery L, Shek P
Protein Sci. - 2020
volume 29, issue 3, pages 789-802
PMID: 31930600; PMCID: PMC7020997
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|470.1345.peg.3020 |
| GenBank: | AKA32782.1 |
| PGAT: | acinetobacter:ABUW_3069 |
| RefSeq: | WP_000834616.1 |
| UniProt: | V5VHL3 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKSFKVALAQ FSPHIGNIDS NTQKMIEQAN QAKKQDADLI IFPELSVIGY PAEDLLLRPN LNKRMQKAFA QLSEVKDIVM VFGFVNQTED GQRYNSAAVM KDGQVLGVFN KHNLPNYGVF DEKRYFQKGH QHLVFEYLGH KFGVLICEDI WSINTVKQLS QLNVDTVLVL NSSPYEVGKP QHRKQTLSEL AKQLHLNIVY VNQVGGQDDL IFDGTSFVSN QNGEIALQAP SFKEDLYIAE FDRDTKLYKV VESAPALETF AEIYQGLVMA TRDYVERSGF PGVILGLSGG IDSALTLAIA VDAIGAERVQ AVMMPYTYTS QISVEDAAEQ ARRMGVTFGI AEIHSIVNSF MQTLYPFFGN SPADATEENL QARARGTLLM GLSNKFGNLV LSTGNKSELS VGYCTLYGDM VGGFAVLKDV YKTIVFELAK YRNSLSETPV IPERVITRPP SAELRPDQKD QDSLPAYDVL DAILYAYIEE DLGQADIIAK GFDKEVVEKV IRLVDRNEYK RRQGAIGPRI TSRAFSRERR YPIVNGWTAN D
NT Sequence
ATGAAAAGTT TTAAAGTTGC CCTTGCTCAG TTTTCTCCGC ATATTGGCAA TATTGACTCA AACACCCAAA AGATGATTGA GCAAGCAAAT CAGGCGAAAA AACAAGACGC TGACCTGATT ATTTTTCCTG AGCTTTCTGT GATTGGTTAT CCAGCTGAAG ACTTATTGTT ACGTCCAAAT TTAAATAAGC GGATGCAAAA GGCTTTTGCT CAACTTAGTG AAGTTAAAGA TATTGTGATG GTTTTCGGCT TCGTAAACCA GACAGAAGAT GGCCAACGTT ATAATTCAGC TGCTGTCATG AAAGACGGTC AGGTTTTAGG TGTCTTCAAT AAACATAACT TGCCAAACTA TGGCGTTTTT GATGAAAAAC GTTATTTCCA AAAAGGCCAT CAGCACTTGG TATTTGAGTA CTTAGGCCAT AAGTTTGGTG TACTCATTTG TGAAGATATC TGGTCAATCA ATACAGTAAA ACAATTAAGC CAATTAAATG TTGATACTGT ACTTGTGCTT AATTCATCGC CATATGAAGT GGGTAAACCA CAGCATCGTA AACAGACATT AAGTGAGCTT GCAAAGCAGC TTCATTTAAA TATTGTTTAT GTTAACCAAG TTGGTGGACA AGATGATTTA ATTTTTGATG GTACAAGTTT TGTTAGCAAT CAAAATGGTG AAATTGCTTT ACAAGCACCT AGCTTTAAAG AAGATCTCTA TATTGCTGAA TTTGACCGCG ATACAAAACT ATATAAAGTT GTCGAGTCTG CTCCTGCATT AGAAACTTTT GCAGAGATCT ATCAAGGCCT AGTCATGGCA ACACGTGACT ATGTAGAACG CTCAGGTTTT CCTGGAGTAA TTTTAGGTCT TTCTGGTGGT ATTGACTCTG CCCTTACGCT TGCTATCGCA GTTGATGCAA TCGGTGCAGA GAGAGTTCAA GCTGTGATGA TGCCATATAC TTACACTTCT CAAATTAGTG TAGAAGATGC AGCGGAACAA GCTCGCCGTA TGGGCGTGAC TTTCGGTATT GCCGAAATTC ATTCAATCGT AAATAGCTTC ATGCAAACTT TGTATCCGTT CTTTGGTAAT TCACCAGCCG ATGCGACTGA AGAAAATTTA CAAGCGCGCG CTCGTGGCAC ATTGCTTATG GGCTTATCTA ACAAGTTTGG TAATTTAGTT CTTTCTACAG GTAATAAATC TGAACTTTCA GTTGGCTATT GCACACTTTA CGGTGACATG GTGGGTGGCT TTGCTGTTCT CAAAGATGTT TATAAAACAA TCGTGTTTGA ATTAGCAAAA TACCGTAATA GCTTAAGTGA AACTCCGGTT ATTCCTGAGC GTGTAATTAC TCGCCCACCT TCAGCAGAAC TGCGTCCAGA TCAAAAAGAC CAAGACTCTT TACCAGCGTA TGATGTATTG GATGCTATCC TTTATGCATA CATTGAGGAA GATTTAGGTC AGGCAGACAT TATTGCCAAA GGTTTTGATA AAGAAGTTGT TGAAAAAGTC ATCCGTTTAG TTGATCGTAA TGAATATAAA CGTCGTCAAG GTGCGATTGG CCCGAGAATT ACTTCTCGTG CATTTAGTCG CGAACGACGT TATCCGATTG TCAACGGTTG GACAGCGAAT GACTGA
Details for AcbaC.18002.a.B1.GE38871
| HARVESTED ON: | 1/29/2015 |
| SEQUENCED ON: | 1/15/2015 |
| EXPECTED MW: | 61kDa |
| OBSERVED MW: | 61kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMK SFKVALAQFS PHIGNIDSNT QKMIEQANQA KKQDADLIIF PELSVIGYPA EDLLLRPNLN KRMQKAFAQL SEVKDIVMVF GFVNQTEDGQ RYNSAAVMKD GQVLGVFNKH NLPNYGVFDE KRYFQKGHQH LVFEYLGHKF GVLICEDIWS INTVKQLSQL NVDTVLVLNS SPYEVGKPQH RKQTLSELAK QLHLNIVYVN QVGGQDDLIF DGTSFVSNQN GEIALQAPSF KEDLYIAEFD RDTKLYKVVE SAPALETFAE IYQGLVMATR DYVERSGFPG VILGLSGGID SALTLAIAVD AIGAERVQAV MMPYTYTSQI SVEDAAEQAR RMGVTFGIAE IHSIVNSFMQ TLYPFFGNSP ADATEENLQA RARGTLLMGL SNKFGNLVLS TGNKSELSVG YCTLYGDMVG GFAVLKDVYK TIVFELAKYR NSLSETPVIP ERVITRPPSA ELRPDQKDQD SLPAYDVLDA ILYAYIEEDL GQADIIAKGF DKEVVEKVIR LVDRNEYKRR QGAIGPRITS RAFSRERRYP IVNGWTAND
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaaa gttttaaagt tgcccttgct cagttttctc cgcatattgg caatattgac tcaaacaccc aaaagatgat tgagcaagca aatcaggcga aaaaacaaga cgctgacctg attatttttc ctgagctttc tgtgattggt tatccagctg aagacttatt gttacgtcca aatttaaata agcggatgca aaaggctttt gctcaactta gtgaagttaa agatattgtg atggttttcg gcttcgtaaa ccagacagaa gatggccaac gttataattc agctgctgtc atgaaagacg gtcaggtttt aggtgtcttc aataaacata acttgccaaa ctatggcgtt tttgatgaaa aacgttattt ccaaaaaggc catcagcact tggtatttga gtacttaggc cataagtttg gtgtactcat ttgtgaagat atctggtcaa tcaatacagt aaaacaatta agccaattaa atgttgatac tgtacttgtg cttaattcat cgccatatga agtgggtaaa ccacagcatc gtaaacagac attaagtgag cttgcaaagc agcttcattt aaatattgtt tatgttaacc aagttggtgg acaagatgat ttaatttttg atggtacaag ttttgttagc aatcaaaatg gtgaaattgc tttacaagca cctagcttta aagaagatct ctatattgct gaatttgacc gcgatacaaa actatataaa gttgtcgagt ctgctcctgc attagaaact tttgcagaga tctatcaagg cctagtcatg gcaacacgtg actatgtaga acgctcaggt tttcctggag taattttagg tctttctggt ggtattgact ctgcccttac gcttgctatc gcagttgatg caatcggtgc agagagagtt caagctgtga tgatgccata tacttacact tctcaaatta gtgtagaaga tgcagcggaa caagctcgcc gtatgggcgt gactttcggt attgccgaaa ttcattcaat cgtaaatagc ttcatgcaaa ctttgtatcc gttctttggt aattcaccag ccgatgcgac tgaagaaaat ttacaagcgc gcgctcgtgg cacattgctt atgggcttat ctaacaagtt tggtaattta gttctttcta caggtaataa atctgaactt tcagttggct attgcacact ttacggtgac atggtgggtg gctttgctgt tctcaaagat gtttataaaa caatcgtgtt tgaattagca aaataccgta atagcttaag tgaaactccg gttattcctg agcgtgtaat tactcgccca ccttcagcag aactgcgtcc agatcaaaaa gaccaagact ctttaccagc gtatgatgta ttggatgcta tcctttatgc atacattgag gaagatttag gtcaggcaga cattattgcc aaaggttttg ataaagaagt tgttgaaaaa gtcatccgtt tagttgatcg taatgaatat aaacgtcgtc aaggtgcgat tggcccgaga attacttctc gtgcatttag tcgcgaacga cgttatccga ttgtcaacgg ttggacagcg aatgactgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t