AcbaC.17999.a

adenylosuccinate lyase

CENTER ID:	AcbaC.17999.a
ORGANISM:	Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AcbaC.17999.a.B1.GE38869	full length		1	462

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AcbaC.17999.a.B1.PW37706	full length		1	462

External Resources

RESOURCE	REFERENCE ID
GenBank:	AKA30874.1
PATRIC ID:	fig\|470.1345.peg.1087
PGAT:	acinetobacter:ABUW_1122
RefSeq:	WP_000993084.1
UniProt:	A0A0D5YFW7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNALTALSPL DGRYASKCDA LRPFLSEFGL IHARVTVEVR WLQALSNRPE IVEVAPFSTE TNAALDAIVS NFSEEDANRI KEIERTTNHD VKAVEYFLKE KIAGIAELQN AGEFIHFACT SEDINNLSHA LMLKNGREVL VSSMKQILNA ISALATTHAE QPMLSRTHGQ TASPTTLGKE MANVAYRLAR QIKQFENVEL LGKINGAVGN YNAHLSAYPN VDWPAHSQAF VESLGLTFNP YTTQIEPHDY MAELFDALRR FNTILIDFNR DVWGYISLGY FKQKLKEGEV GSSTMPHKVN PIDFENSEGN LGIANAVLAH LGEKLPISRW QRDLTDSTVL RNMGVGFAQS LIAFDACLKG IGKLELNANR LNEDLDQAQE VLAEPIQTVM RRYNVEKPYE KLKALTRGQA MTRDMMVNFV NGNELAQVPS EERARLAELT PATYTGNAAE QAKQINELIS KI

NT Sequence

ATGAACGCTT TAACCGCACT ATCACCATTA GATGGACGTT ATGCCAGCAA ATGTGATGCG CTACGCCCTT TTCTTTCTGA GTTTGGTTTA ATTCATGCTC GTGTCACTGT GGAAGTGCGT TGGTTACAAG CGCTTTCTAA CCGTCCGGAA ATTGTTGAAG TTGCTCCTTT CTCAACTGAA ACAAATGCAG CTCTAGATGC AATCGTAAGC AACTTCTCTG AAGAAGATGC GAACCGCATT AAAGAAATTG AACGCACAAC TAACCATGAC GTAAAAGCAG TTGAATACTT CTTGAAAGAG AAAATTGCAG GTATTGCTGA ATTACAAAAT GCAGGTGAGT TCATTCACTT TGCTTGTACA TCAGAAGACA TCAATAACTT GTCTCATGCA CTTATGCTTA AAAACGGTCG TGAAGTTTTA GTATCAAGCA TGAAGCAAAT TTTAAATGCA ATCTCTGCTT TAGCTACAAC TCATGCTGAA CAACCGATGT TGTCTCGTAC ACATGGTCAA ACTGCTAGCC CAACAACTTT GGGTAAAGAA ATGGCGAACG TTGCATATCG TTTAGCTCGC CAAATCAAAC AATTTGAAAA TGTTGAATTA TTAGGCAAAA TCAATGGTGC AGTAGGTAAC TACAATGCTC ACCTTTCTGC TTATCCAAAT GTGGATTGGC CAGCGCATTC ACAAGCTTTT GTTGAATCTT TAGGTTTAAC ATTTAATCCA TATACAACTC AAATTGAGCC ACACGACTAC ATGGCTGAAT TATTTGACGC GTTACGTCGT TTCAATACAA TTTTGATCGA CTTTAACCGT GACGTATGGG GTTATATCTC TTTAGGCTAC TTCAAGCAAA AACTTAAAGA AGGTGAAGTT GGCTCATCAA CTATGCCACA TAAAGTTAAC CCGATCGACT TTGAAAACTC TGAAGGTAAC TTAGGCATTG CAAATGCAGT GTTGGCTCAC TTAGGCGAAA AATTACCAAT TTCTCGCTGG CAGCGTGACT TAACTGACTC AACTGTACTT CGTAACATGG GTGTTGGTTT TGCTCAAAGC TTAATTGCTT TTGATGCTTG CTTAAAAGGT ATTGGTAAAC TTGAACTCAA TGCAAACCGC TTAAATGAAG ATCTTGACCA AGCTCAAGAA GTTCTTGCAG AGCCAATTCA AACAGTTATG CGTCGTTATA ACGTTGAAAA ACCATATGAG AAGTTAAAAG CATTAACTCG TGGTCAAGCA ATGACGCGTG ACATGATGGT TAACTTCGTA AATGGTAACG AACTTGCTCA AGTACCAAGT GAAGAGCGCG CCCGCTTAGC TGAGCTTACA CCTGCAACTT ACACAGGTAA TGCTGCTGAA CAAGCTAAAC AAATTAACGA ATTAATTAGC AAAATCTAA

Details for AcbaC.17999.a.B1.GE38869

HARVESTED ON:	1/29/2015
SEQUENCED ON:	1/15/2015
EXPECTED MW:	52kDa
OBSERVED MW:	48kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMN ALTALSPLDG RYASKCDALR PFLSEFGLIH ARVTVEVRWL QALSNRPEIV EVAPFSTETN AALDAIVSNF SEEDANRIKE IERTTNHDVK AVEYFLKEKI AGIAELQNAG EFIHFACTSE DINNLSHALM LKNGREVLVS SMKQILNAIS ALATTHAEQP MLSRTHGQTA SPTTLGKEMA NVAYRLARQI KQFENVELLG KINGAVGNYN AHLSAYPNVD WPAHSQAFVE SLGLTFNPYT TQIEPHDYMA ELFDALRRFN TILIDFNRDV WGYISLGYFK QKLKEGEVGS STMPHKVNPI DFENSEGNLG IANAVLAHLG EKLPISRWQR DLTDSTVLRN MGVGFAQSLI AFDACLKGIG KLELNANRLN EDLDQAQEVL AEPIQTVMRR YNVEKPYEKL KALTRGQAMT RDMMVNFVNG NELAQVPSEE RARLAELTPA TYTGNAAEQA KQINELISKI

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaacg ctttaaccgc actatcacca ttagatggac gttatgccag caaatgtgat gcgctacgcc cttttctttc tgagtttggt ttaattcatg ctcgtgtcac tgtggaagtg cgttggttac aagcgctttc taaccgtccg gaaattgttg aagttgctcc tttctcaact gaaacaaatg cagctctaga tgcaatcgta agcaacttct ctgaagaaga tgcgaaccgc attaaagaaa ttgaacgcac aactaaccat gacgtaaaag cagttgaata cttcttgaaa gagaaaattg caggtattgc tgaattacaa aatgcaggtg agttcattca ctttgcttgt acatcagaag acatcaataa cttgtctcat gcacttatgc ttaaaaacgg tcgtgaagtt ttagtatcaa gcatgaagca aattttaaat gcaatctctg ctttagctac aactcatgct gaacaaccga tgttgtctcg tacacatggt caaactgcta gcccaacaac tttgggtaaa gaaatggcga acgttgcata tcgtttagct cgccaaatca aacaatttga aaatgttgaa ttattaggca aaatcaatgg tgcagtaggt aactacaatg ctcacctttc tgcttatcca aatgtggatt ggccagcgca ttcacaagct tttgttgaat ctttaggttt aacatttaat ccatatacaa ctcaaattga gccacacgac tacatggctg aattatttga cgcgttacgt cgtttcaata caattttgat cgactttaac cgtgacgtat ggggttatat ctctttaggc tacttcaagc aaaaacttaa agaaggtgaa gttggctcat caactatgcc acataaagtt aacccgatcg actttgaaaa ctctgaaggt aacttaggca ttgcaaatgc agtgttggct cacttaggcg aaaaattacc aatttctcgc tggcagcgtg acttaactga ctcaactgta cttcgtaaca tgggtgttgg ttttgctcaa agcttaattg cttttgatgc ttgcttaaaa ggtattggta aacttgaact caatgcaaac cgcttaaatg aagatcttga ccaagctcaa gaagttcttg cagagccaat tcaaacagtt atgcgtcgtt ataacgttga aaaaccatat gagaagttaa aagcattaac tcgtggtcaa gcaatgacgc gtgacatgat ggttaacttc gtaaatggta acgaacttgc tcaagtacca agtgaagagc gcgcccgctt agctgagctt acacctgcaa cttacacagg taatgctgct gaacaagcta aacaaattaa cgaattaatt agcaaaatct gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat

Details for AcbaC.17999.a.B1.PW37706

PURIFICATION DATe:	4/29/2015
CONCENTRATION:	34mg/ml
OBSERVED MW:	52kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	17
VIAL VOLUME:	200µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAACG CTTTAACCGC ACTATCACCA TTAGATGGAC GTTATGCCAG CAAATGTGAT GCGCTACGCC CTTTTCTTTC TGAGTTTGGT TTAATTCATG CTCGTGTCAC TGTGGAAGTG CGTTGGTTAC AAGCGCTTTC TAACCGTCCG GAAATTGTTG AAGTTGCTCC TTTCTCAACT GAAACAAATG CAGCTCTAGA TGCAATCGTA AGCAACTTCT CTGAAGAAGA TGCGAACCGC ATTAAAGAAA TTGAACGCAC AACTAACCAT GACGTAAAAG CAGTTGAATA CTTCTTGAAA GAGAAAATTG CAGGTATTGC TGAATTACAA AATGCAGGTG AGTTCATTCA CTTTGCTTGT ACATCAGAAG ACATCAATAA CTTGTCTCAT GCACTTATGC TTAAAAACGG TCGTGAAGTT TTAGTATCAA GCATGAAGCA AATTTTAAAT GCAATCTCTG CTTTAGCTAC AACTCATGCT GAACAACCGA TGTTGTCTCG TACACATGGT CAAACTGCTA GCCCAACAAC TTTGGGTAAA GAAATGGCGA ACGTTGCATA TCGTTTAGCT CGCCAAATCA AACAATTTGA AAATGTTGAA TTATTAGGCA AAATCAATGG TGCAGTAGGT AACTACAATG CTCACCTTTC TGCTTATCCA AATGTGGATT GGCCAGCGCA TTCACAAGCT TTTGTTGAAT CTTTAGGTTT AACATTTAAT CCATATACAA CTCAAATTGA GCCACACGAC TACATGGCTG AATTATTTGA CGCGTTACGT CGTTTCAATA CAATTTTGAT CGACTTTAAC CGTGACGTAT GGGGTTATAT CTCTTTAGGC TACTTCAAGC AAAAACTTAA AGAAGGTGAA GTTGGCTCAT CAACTATGCC ACATAAAGTT AACCCGATCG ACTTTGAAAA CTCTGAAGGT AACTTAGGCA TTGCAAATGC AGTGTTGGCT CACTTAGGCG AAAAATTACC AATTTCTCGC TGGCAGCGTG ACTTAACTGA CTCAACTGTA CTTCGTAACA TGGGTGTTGG TTTTGCTCAA AGCTTAATTG CTTTTGATGC TTGCTTAAAA GGTATTGGTA AACTTGAACT CAATGCAAAC CGCTTAAATG AAGATCTTGA CCAAGCTCAA GAAGTTCTTG CAGAGCCAAT TCAAACAGTT ATGCGTCGTT ATAACGTTGA AAAACCATAT GAGAAGTTAA AAGCATTAAC TCGTGGTCAA GCAATGACGC GTGACATGAT GGTTAACTTC GTAAATGGTA ACGAACTTGC TCAAGTACCA AGTGAAGAGC GCGCCCGCTT AGCTGAGCTT ACACCTGCAA CTTACACAGG TAATGCTGCT GAACAAGCTA AACAAATTAA CGAATTAATT AGCAAAATCT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT