AcbaC.17996.a

lipopolysaccharide transport periplasmic protein LptA

CENTER ID: AcbaC.17996.a
ORGANISM: Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS: cloned
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AcbaC.17996.a.B2.GE38868 mature protein 33 182
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|470.1345.peg.2600
GenBank: AKA32368.1
PGAT: acinetobacter:ABUW_2646
RefSeq: WP_000554244.1
UniProt: V5VEZ9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MHQSLNLKRS SAFLKQAAVI TFVALSSAST FALPSDRNQQ ISLVADRATY NEKTGLTTYT GNVVIEQGTM KLQADSIVAT LNSKREIQTI TAKGRPSKFQ QQISADKGIA RGEGQTIVYN ADTGIITLSG GAYLYQDGSS IRGNTLKYSM NKGDVEAQGS SSNRVQIIIP PSSSKSFPGA RD
NT Sequence
ATGCACCAAA GTCTTAATTT AAAACGTTCT TCAGCTTTCC TAAAACAAGC TGCGGTAATT ACATTCGTTG CTTTATCATC TGCTTCGACT TTTGCTTTGC CGTCTGACCG TAATCAACAA ATTTCATTAG TGGCAGACCG AGCAACTTAT AATGAAAAAA CCGGTTTGAC GACTTATACG GGTAATGTCG TGATTGAGCA GGGCACCATG AAGCTTCAGG CCGACTCAAT TGTTGCTACG TTAAACTCTA AACGTGAAAT TCAAACGATC ACTGCTAAAG GTAGACCGTC TAAGTTTCAG CAACAAATAA GTGCTGATAA AGGTATTGCA CGCGGTGAAG GACAAACGAT TGTTTATAAT GCAGATACAG GTATTATTAC CTTGTCTGGC GGTGCATATT TATACCAAGA TGGTTCAAGT ATTCGCGGTA ACACCCTGAA ATATAGTATG AATAAGGGTG ATGTTGAAGC TCAAGGTTCT TCTTCAAACC GTGTACAAAT CATTATTCCA CCTTCAAGTT CAAAAAGTTT CCCAGGAGCG CGTGACTAA
Details for AcbaC.17996.a.B2.GE38868
HARVESTED ON: data unavailable
SEQUENCED ON: 1/15/2015
EXPECTED MW: 17kDa
OBSERVED MW: 104kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 101
Validated AA Sequence
MAHHHHHHHM LPSDRNQQIS LVADRATYNE KTGLTTYTGN VVIEQGTMKL QADSIVATLN SKREIQTITA KGRPSKFQQQ ISADKGIARG EGQTIVYNAD TGIITLSGGA YLYQDGSSIR GNTLKYSMNK GDVEAQGSSS NRVQIIIPPS SSKSFPGARD
Validated NT Sequence
atcttactca cttagtcacg cgctcctggg aaactttttg aacttgaagg tggaataatg atttgtacac ggtttgaaga agaaccttga gcttcaacat cacccttatt catactatat ttcagggtgt taccgcgaat acttgaacca tcttggtata aatatgcacc gccagacaag gtaataatac ctgtatctgc attataaaca atcgtttgtc cttcaccgcg tgcaatacct ttatcagcac ttatttgttg ctgaaactta gacggtctac ctttagcagt gatcgtttga atttcacgtt tagagtttaa cgtagcaaca attgagtcgg cctgaagctt catggtgccc tgctcaatca cgacattacc cgtataagtc gtcaaaccgg ttttttcatt ataagttgct cggtctgcca ctaatgaaat ttgttgatta cggtcagacg gcaacatatg gtggtggtgg tggtggtgag ccat
Expected Protein Sequence
MAHHHHHHLP SDRNQQISLV ADRATYNEKT GLTTYTGNVV IEQGTMKLQA DSIVATLNSK REIQTITAKG RPSKFQQQIS ADKGIARGEG QTIVYNADTG IITLSGGAYL YQDGSSIRGN TLKYSMNKGD VEAQGSSSNR VQIIIPPSSS KSFPGARD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttgccgt ctgaccgtaa tcaacaaatt tcattagtgg cagaccgagc aacttataat gaaaaaaccg gtttgacgac ttatacgggt aatgtcgtga ttgagcaggg caccatgaag cttcaggccg actcaattgt tgctacgtta aactctaaac gtgaaattca aacgatcact gctaaaggta gaccgtctaa gtttcagcaa caaataagtg ctgataaagg tattgcacgc ggtgaaggac aaacgattgt ttataatgca gatacaggta ttattacctt gtctggcggt gcatatttat accaagatgg ttcaagtatt cgcggtaaca ccctgaaata tagtatgaat aagggtgatg ttgaagctca aggttcttct tcaaaccgtg tacaaatcat tattccacct tcaagttcaa aaagtttccc aggagcgcgt gactgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat