AcbaC.17994.a

succinyl-diaminopimelate desuccinylase

CENTER ID: AcbaC.17994.a
ORGANISM: Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AcbaC.17994.a.B1.GE38867 full length 1 377
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AcbaC.17994.a.B1.PS02401 full length 1 377

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|470.1345.peg.656
GenBank: AKA30455.1
PGAT: acinetobacter:ABUW_0686
RefSeq: WP_001016570.1
UniProt: A0A0D5YDV4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNHSDTLSLS LELLEQPSVT PIDHTCQTIM ADRLAKVGFH IEPMRFGDVD NLWARRGTEG PVFCFAGHTD VVPTGRLDAW NSDPFAPEIR DGKLYGRGSA DMKTALAAMV VASERFVAKH PNHKGSIAFL ITSDEEGPAV NGTVKVIETL EKRNEKITWC LVGEPSSTHK LGDIVKNGRR GSLNAVLKVQ GKQGHVAYPH LARNPIHEAS PALAELCQTV WDNGNEYFPA TSFQISNIHA GTGATNVIPG ALEVTFNFRY STEVTAEQLK QRVHEILDKH GLQYEIVWNL SGLPFLTPVG ELVNAAQTAI LNVTGTETEL STSGGTSDGR FIAPTGAQVL ELGVLNATIH QINEHVDVHD LDPLTDIYEQ ILENLLA
NT Sequence
ATGAACCACT CAGACACTCT CTCGCTTAGC TTAGAACTTT TAGAACAGCC TTCTGTTACC CCTATTGATC ATACTTGCCA AACTATTATG GCTGACCGCT TAGCTAAAGT TGGCTTCCAT ATTGAACCTA TGCGCTTTGG AGATGTCGAT AATTTATGGG CTCGACGTGG AACCGAAGGC CCTGTATTTT GTTTTGCTGG TCATACCGAC GTAGTACCAA CAGGACGTTT AGACGCTTGG AATTCAGACC CATTTGCACC AGAAATTCGC GATGGCAAAT TATATGGTCG TGGTTCAGCA GATATGAAAA CTGCATTAGC TGCAATGGTA GTTGCATCTG AACGTTTTGT AGCAAAACAC CCAAACCATA AAGGTTCAAT TGCTTTTTTA ATTACTTCAG ATGAAGAAGG TCCTGCTGTT AACGGTACCG TTAAAGTCAT CGAAACACTG GAAAAGCGTA ATGAAAAAAT TACTTGGTGT TTGGTTGGTG AACCATCAAG TACTCATAAA TTAGGTGATA TTGTTAAGAA TGGCCGTCGT GGTTCTCTTA ATGCTGTATT GAAGGTTCAA GGCAAACAAG GTCATGTAGC TTACCCACAC TTGGCACGTA ATCCAATTCA TGAAGCATCT CCAGCGTTAG CTGAGTTGTG CCAAACAGTT TGGGACAATG GCAATGAGTA TTTCCCTGCA ACTTCATTCC AGATTTCAAA TATCCATGCA GGTACTGGCG CAACCAATGT AATCCCTGGT GCTTTAGAAG TTACTTTTAA TTTCCGTTAT TCAACCGAGG TTACAGCAGA ACAGCTTAAA CAACGTGTTC ATGAAATTTT AGACAAGCAT GGCTTGCAGT ATGAAATTGT CTGGAATTTG TCTGGCTTAC CATTCTTAAC TCCAGTAGGT GAATTAGTTA ACGCAGCTCA AACTGCTATT TTAAATGTGA CTGGTACTGA GACTGAGCTT TCAACAAGTG GTGGTACATC TGATGGACGC TTCATTGCTC CAACAGGTGC ACAAGTTTTA GAACTCGGTG TTCTAAACGC TACTATCCAT CAAATTAATG AACATGTTGA TGTACATGAT CTTGATCCAT TAACTGATAT TTATGAGCAA ATTTTAGAGA ACCTACTTGC TTAA
Details for AcbaC.17994.a.B1.GE38867
HARVESTED ON: 1/29/2015
SEQUENCED ON: 1/15/2015
EXPECTED MW: 42kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 97
PERCENT COVERAGE: 71
Validated AA Sequence
MAHHHHHHMN HSDTLSLSLE LLEQPSVTPI DHTCQTIMAD RLAKVGFHIE PMRFGDVDNL WARRGTEGPV FCFAGHTDVV PTGRLDAWNS DPFAPEIRDG KLYGRGSADM KTALAAMVVA SERFVAKHPN HKGSIAFLIT SDEEGPAVNG TVKVIETLEK RNEKITWCLV GEPSSTHKLG DIVKNGRRGS LNAVLKVQGK QGHVAYPHLA RNPIHEASPA LAELCQTVWD NGNEYFPATS FQIXXIHAGT GATNVIPGAL EVTFNFRYSX XGYSRTA
Validated NT Sequence
tctctaannn tnnntcataa atatcagtta atggatcaag atcatgtaca tcaacatgtt cattaatttg atggatagta gcgtttagaa caccgagttc taaaacttgt gcacctgttg gagcaatgaa gcgtccatca gatgtaccac cacttgttga aagctcagtc tcagtaccag tcacatttaa aatagcagtt tgagctgcgt taactaattc acctactgga gttaagaatg gtaagccaga caaattccag acaatttcat actgcaagcc atgcttgtct aaaatttcat gaacacgttg tttaagctgt tctgctgtaa ccnnnnnnng aataacggaa attaaaagta acttctaaag caccagggat tacattggtt gcgccagtac ctgcatggat annnnaaatc tggaatgaag ttgcagggaa atactcattg ccattgtccc aaactgtttg gcacaactca gctaacgctg gagatgcttc atgaattgga ttacgtgcca agtgtgggta agctacatga ccttgtttgc cttgaacctt caatacagca ttaagagaac cacgacggcc attcttaaca atatcaccta atttatgagt acttgatggt tcaccaacca aacaccaagt aattttttca ttacgctttt ccagtgtttc gatgacttta acggtaccgt taacagcagg accttcttca tctgaagtaa ttaaaaaagc aattgaacct ttatggtttg ggtgttttgc tacaaaacgt tcagatgcaa ctaccattgc agctaatgca gttttcatat ctgctgaacc acgaccatat aatttgccat cgcgaatttc tggtgcaaat gggtctgaat tccaagcgtc taaacgtcct gttggtacta cgtcggtatg accagcaaaa caaaatacag ggccttcggt tccacgtcga gcccataaat tatcgacatc tccaaagcgc ataggttcaa tatggaagcc aactttagct aagcggtcag ccataatagt ttggcaagta tgatcaatag gggtaacaga aggctgttct aaaagttcta agctaagcga gagagtgtct gagtggttca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHMN HSDTLSLSLE LLEQPSVTPI DHTCQTIMAD RLAKVGFHIE PMRFGDVDNL WARRGTEGPV FCFAGHTDVV PTGRLDAWNS DPFAPEIRDG KLYGRGSADM KTALAAMVVA SERFVAKHPN HKGSIAFLIT SDEEGPAVNG TVKVIETLEK RNEKITWCLV GEPSSTHKLG DIVKNGRRGS LNAVLKVQGK QGHVAYPHLA RNPIHEASPA LAELCQTVWD NGNEYFPATS FQISNIHAGT GATNVIPGAL EVTFNFRYST EVTAEQLKQR VHEILDKHGL QYEIVWNLSG LPFLTPVGEL VNAAQTAILN VTGTETELST SGGTSDGRFI APTGAQVLEL GVLNATIHQI NEHVDVHDLD PLTDIYEQIL ENLLA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaacc actcagacac tctctcgctt agcttagaac ttttagaaca gccttctgtt acccctattg atcatacttg ccaaactatt atggctgacc gcttagctaa agttggcttc catattgaac ctatgcgctt tggagatgtc gataatttat gggctcgacg tggaaccgaa ggccctgtat tttgttttgc tggtcatacc gacgtagtac caacaggacg tttagacgct tggaattcag acccatttgc accagaaatt cgcgatggca aattatatgg tcgtggttca gcagatatga aaactgcatt agctgcaatg gtagttgcat ctgaacgttt tgtagcaaaa cacccaaacc ataaaggttc aattgctttt ttaattactt cagatgaaga aggtcctgct gttaacggta ccgttaaagt catcgaaaca ctggaaaagc gtaatgaaaa aattacttgg tgtttggttg gtgaaccatc aagtactcat aaattaggtg atattgttaa gaatggccgt cgtggttctc ttaatgctgt attgaaggtt caaggcaaac aaggtcatgt agcttaccca cacttggcac gtaatccaat tcatgaagca tctccagcgt tagctgagtt gtgccaaaca gtttgggaca atggcaatga gtatttccct gcaacttcat tccagatttc aaatatccat gcaggtactg gcgcaaccaa tgtaatccct ggtgctttag aagttacttt taatttccgt tattcaaccg aggttacagc agaacagctt aaacaacgtg ttcatgaaat tttagacaag catggcttgc agtatgaaat tgtctggaat ttgtctggct taccattctt aactccagta ggtgaattag ttaacgcagc tcaaactgct attttaaatg tgactggtac tgagactgag ctttcaacaa gtggtggtac atctgatgga cgcttcattg ctccaacagg tgcacaagtt ttagaactcg gtgttctaaa cgctactatc catcaaatta atgaacatgt tgatgtacat gatcttgatc cattaactga tatttatgag caaattttag agaacctact tgcttgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for AcbaC.17994.a.B1.PS02401
PURIFICATION DATe: 5/19/2015
CONCENTRATION: 102mg/ml
OBSERVED MW: 45kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 200µl
PERCENT IDENTITY: 97
PERCENT COVERAGE: 71
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMN HSDTLSLSLE LLEQPSVTPI DHTCQTIMAD RLAKVGFHIE PMRFGDVDNL WARRGTEGPV FCFAGHTDVV PTGRLDAWNS DPFAPEIRDG KLYGRGSADM KTALAAMVVA SERFVAKHPN HKGSIAFLIT SDEEGPAVNG TVKVIETLEK RNEKITWCLV GEPSSTHKLG DIVKNGRRGS LNAVLKVQGK QGHVAYPHLA RNPIHEASPA LAELCQTVWD NGNEYFPATS FQIXXIHAGT GATNVIPGAL EVTFNFRYSX XGYSRTA
Validated NT Sequence
tctctaannn tnnntcataa atatcagtta atggatcaag atcatgtaca tcaacatgtt cattaatttg atggatagta gcgtttagaa caccgagttc taaaacttgt gcacctgttg gagcaatgaa gcgtccatca gatgtaccac cacttgttga aagctcagtc tcagtaccag tcacatttaa aatagcagtt tgagctgcgt taactaattc acctactgga gttaagaatg gtaagccaga caaattccag acaatttcat actgcaagcc atgcttgtct aaaatttcat gaacacgttg tttaagctgt tctgctgtaa ccnnnnnnng aataacggaa attaaaagta acttctaaag caccagggat tacattggtt gcgccagtac ctgcatggat annnnaaatc tggaatgaag ttgcagggaa atactcattg ccattgtccc aaactgtttg gcacaactca gctaacgctg gagatgcttc atgaattgga ttacgtgcca agtgtgggta agctacatga ccttgtttgc cttgaacctt caatacagca ttaagagaac cacgacggcc attcttaaca atatcaccta atttatgagt acttgatggt tcaccaacca aacaccaagt aattttttca ttacgctttt ccagtgtttc gatgacttta acggtaccgt taacagcagg accttcttca tctgaagtaa ttaaaaaagc aattgaacct ttatggtttg ggtgttttgc tacaaaacgt tcagatgcaa ctaccattgc agctaatgca gttttcatat ctgctgaacc acgaccatat aatttgccat cgcgaatttc tggtgcaaat gggtctgaat tccaagcgtc taaacgtcct gttggtacta cgtcggtatg accagcaaaa caaaatacag ggccttcggt tccacgtcga gcccataaat tatcgacatc tccaaagcgc ataggttcaa tatggaagcc aactttagct aagcggtcag ccataatagt ttggcaagta tgatcaatag gggtaacaga aggctgttct aaaagttcta agctaagcga gagagtgtct gagtggttca tatggtggtg gtggtggtga gccat
Expressed Protein Sequence
MAHHHHHHMN HSDTLSLSLE LLEQPSVTPI DHTCQTIMAD RLAKVGFHIE PMRFGDVDNL WARRGTEGPV FCFAGHTDVV PTGRLDAWNS DPFAPEIRDG KLYGRGSADM KTALAAMVVA SERFVAKHPN HKGSIAFLIT SDEEGPAVNG TVKVIETLEK RNEKITWCLV GEPSSTHKLG DIVKNGRRGS LNAVLKVQGK QGHVAYPHLA RNPIHEASPA LAELCQTVWD NGNEYFPATS FQISNIHAGT GATNVIPGAL EVTFNFRYST EVTAEQLKQR VHEILDKHGL QYEIVWNLSG LPFLTPVGEL VNAAQTAILN VTGTETELST SGGTSDGRFI APTGAQVLEL GVLNATIHQI NEHVDVHDLD PLTDIYEQIL ENLLA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAACC ACTCAGACAC TCTCTCGCTT AGCTTAGAAC TTTTAGAACA GCCTTCTGTT ACCCCTATTG ATCATACTTG CCAAACTATT ATGGCTGACC GCTTAGCTAA AGTTGGCTTC CATATTGAAC CTATGCGCTT TGGAGATGTC GATAATTTAT GGGCTCGACG TGGAACCGAA GGCCCTGTAT TTTGTTTTGC TGGTCATACC GACGTAGTAC CAACAGGACG TTTAGACGCT TGGAATTCAG ACCCATTTGC ACCAGAAATT CGCGATGGCA AATTATATGG TCGTGGTTCA GCAGATATGA AAACTGCATT AGCTGCAATG GTAGTTGCAT CTGAACGTTT TGTAGCAAAA CACCCAAACC ATAAAGGTTC AATTGCTTTT TTAATTACTT CAGATGAAGA AGGTCCTGCT GTTAACGGTA CCGTTAAAGT CATCGAAACA CTGGAAAAGC GTAATGAAAA AATTACTTGG TGTTTGGTTG GTGAACCATC AAGTACTCAT AAATTAGGTG ATATTGTTAA GAATGGCCGT CGTGGTTCTC TTAATGCTGT ATTGAAGGTT CAAGGCAAAC AAGGTCATGT AGCTTACCCA CACTTGGCAC GTAATCCAAT TCATGAAGCA TCTCCAGCGT TAGCTGAGTT GTGCCAAACA GTTTGGGACA ATGGCAATGA GTATTTCCCT GCAACTTCAT TCCAGATTTC AAATATCCAT GCAGGTACTG GCGCAACCAA TGTAATCCCT GGTGCTTTAG AAGTTACTTT TAATTTCCGT TATTCAACCG AGGTTACAGC AGAACAGCTT AAACAACGTG TTCATGAAAT TTTAGACAAG CATGGCTTGC AGTATGAAAT TGTCTGGAAT TTGTCTGGCT TACCATTCTT AACTCCAGTA GGTGAATTAG TTAACGCAGC TCAAACTGCT ATTTTAAATG TGACTGGTAC TGAGACTGAG CTTTCAACAA GTGGTGGTAC ATCTGATGGA CGCTTCATTG CTCCAACAGG TGCACAAGTT TTAGAACTCG GTGTTCTAAA CGCTACTATC CATCAAATTA ATGAACATGT TGATGTACAT GATCTTGATC CATTAACTGA TATTTATGAG CAAATTTTAG AGAACCTACT TGCTTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT