SSGCID: Target Page

AcbaC.17993.a

glycyl-tRNA synthetase, alpha subunit

CENTER ID:	AcbaC.17993.a
ORGANISM:	Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AcbaC.17993.a.B1.GE38866	full length		1	325

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AcbaC.17993.a.B1.PW37692	full length		1	325

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|470.1345.peg.353
GenBank:	AKA30153.1
PGAT:	acinetobacter:ABUW_0367
RefSeq:	WP_000085209.1
UniProt:	A0A0D5YE11

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSRAISHIDT FQGLILALQN YWAEQGCVVL QPYDMEMGAG TFHTATFLHA LGPETWNAAY VQPSRRPKDG RYGENPNRLQ HYYQFQVVLK PNPDNIQQLY LDSLKAIGID TLTHDIRFVE DNWESPTLGA WGLGWEVWLN GMEVTQFTYF QQVGGVECYP VTGEITYGLE RLAMYLQGVD SVYDLVWTKG QFGTVTYGDV FHQNEVEQST YNFEYAPVDK LFELFDFYES EANRLMEAKL PLPAYEQVVK ASHTFNLLDA RGAISVTERQ RYILRVRTLA RAIAQSYVQA RAELGFPMAE PHLRDEVLAQ LKAQAESEAA KAEKN

NT Sequence

ATGAGTCGCG CCATCTCGCA CATTGATACA TTTCAGGGCT TAATTCTTGC CCTACAAAAC TATTGGGCCG AACAAGGCTG CGTCGTACTA CAACCTTACG ACATGGAAAT GGGCGCTGGA ACATTCCACA CCGCAACATT CTTACATGCT TTAGGGCCAG AGACTTGGAA CGCGGCATAC GTTCAACCTT CACGTCGTCC AAAAGATGGC CGCTACGGTG AAAACCCGAA CCGTTTGCAA CACTACTATC AGTTCCAAGT GGTACTTAAG CCAAACCCAG ACAACATTCA ACAGCTTTAT CTCGATTCTT TAAAAGCAAT CGGGATTGAT ACGCTTACTC ACGATATCCG TTTCGTAGAA GACAACTGGG AATCTCCAAC TCTTGGGGCT TGGGGCTTAG GTTGGGAAGT TTGGCTTAAC GGTATGGAAG TAACTCAGTT CACTTACTTC CAACAAGTTG GTGGTGTTGA ATGTTACCCA GTAACAGGCG AAATCACTTA CGGTTTAGAA CGTCTTGCAA TGTACCTTCA AGGCGTTGAC TCGGTTTATG ACCTTGTTTG GACGAAAGGC CAATTCGGTA CTGTAACTTA CGGCGACGTA TTCCATCAAA ATGAAGTTGA GCAATCGACT TATAACTTTG AATACGCTCC AGTTGACAAA CTATTTGAAC TATTTGATTT CTACGAGTCA GAAGCTAACC GTTTAATGGA AGCTAAACTT CCACTTCCAG CTTATGAGCA AGTCGTTAAA GCGTCTCATA CCTTTAACTT GCTTGATGCA CGTGGCGCAA TTTCTGTAAC TGAGCGTCAA CGCTACATTT TACGTGTACG TACTTTGGCG CGCGCTATTG CACAAAGTTA TGTACAAGCA CGTGCAGAGC TTGGCTTCCC AATGGCAGAA CCACACTTAC GTGATGAAGT ATTGGCACAG CTAAAAGCAC AAGCTGAAAG TGAAGCAGCA AAGGCGGAGA AAAACTAA

Details for AcbaC.17993.a.B1.GE38866

HARVESTED ON:	1/29/2015
SEQUENCED ON:	1/15/2015
EXPECTED MW:	38kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	93
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMS RAISHIDTFQ GLILALQNYW AEQGCVVLQP YDMEMGAGTF HTATFLHALG PETWNAAYVQ PSRRPKDGRY GENPNRLQHY YQFQVVLKPN PDNIQQLYLD SLKAIGIDTL THDIRFVEDN WESPTLGAWG LGWEVWLNGM EVTQFTYFQQ VGGVECYPVT GEITYGLERL AMYLQGVDSV YDLVWTKGQF GTVTYGDVFH QNEVEQSTYN FEYAPVDKLF ELFDFYESXX NRLMEXKLPL PXYEQVXKAX HTXXLLXXXG AISXTXRQRY ILRXXXLARA IAQSYVXARA ELGFPMAEPH LRDEVLAQLK AQAXXXXXK

Validated NT Sequence

gcctttgnnn nnnnnnnnnn agcttgtgct tttagctgtg ccaatacttc atcacgtaag tgtggttctg ccattgggaa gccaagctct gcacgtgctt ntacataact ttgtgcaata gcgcgcgcca aagnannnnc acgtaaaatg tagcgttgac gntcagtnnc agaaattgcg ccnnnnnnnn caagcaannn naaggtatga gnngctttnn nnacttgctc ataannngga agtggaagtt tancttccat taaacggtta nnnnctgact cgtagaaatc aaatagttca aatagtttgt caactggagc gtattcaaag ttataagtcg attgctcaac ttcattttga tggaatacgt cgccgtaagt tacagtaccg aattggcctt tcgtccaaac aaggtcataa accgagtcaa cgccttgaag gtacattgca agacgttcta aaccgtaagt gatttcgcct gttactgggt aacattcaac accaccaact tgttggaagt aagtgaactg agttacttcc ataccgttaa gccaaacttc ccaacctaag ccccaagccc caagagttgg agattcccag ttgtcttcta cgaaacggat atcgtgagta agcgtatcaa tcccgattgc ttttaaagaa tcgagataaa gctgttgaat gttgtctggg tttggcttaa gtaccacttg gaactgatag tagtgttgca aacggttcgg gttttcaccg tagcggccat cttttggacg acgtgaaggt tgaacgtatg ccgcgttcca agtctctggc cctaaagcat gtaagaatgt tgcggtgtgg aatgttccag cgcccatttc catgtcgtaa ggttgtagta cgacgcagcc ttgttcggcc caatagtttt gtagggcaag aattaagccc tgaaatgtat caatgtgcga gatggcgcga ctcatatggt ggtggtggtg gtgagccat

Expected Protein Sequence

MAHHHHHHMS RAISHIDTFQ GLILALQNYW AEQGCVVLQP YDMEMGAGTF HTATFLHALG PETWNAAYVQ PSRRPKDGRY GENPNRLQHY YQFQVVLKPN PDNIQQLYLD SLKAIGIDTL THDIRFVEDN WESPTLGAWG LGWEVWLNGM EVTQFTYFQQ VGGVECYPVT GEITYGLERL AMYLQGVDSV YDLVWTKGQF GTVTYGDVFH QNEVEQSTYN FEYAPVDKLF ELFDFYESEA NRLMEAKLPL PAYEQVVKAS HTFNLLDARG AISVTERQRY ILRVRTLARA IAQSYVQARA ELGFPMAEPH LRDEVLAQLK AQAESEAAKA EKN

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagtc gcgccatctc gcacattgat acatttcagg gcttaattct tgccctacaa aactattggg ccgaacaagg ctgcgtcgta ctacaacctt acgacatgga aatgggcgct ggaacattcc acaccgcaac attcttacat gctttagggc cagagacttg gaacgcggca tacgttcaac cttcacgtcg tccaaaagat ggccgctacg gtgaaaaccc gaaccgtttg caacactact atcagttcca agtggtactt aagccaaacc cagacaacat tcaacagctt tatctcgatt ctttaaaagc aatcgggatt gatacgctta ctcacgatat ccgtttcgta gaagacaact gggaatctcc aactcttggg gcttggggct taggttggga agtttggctt aacggtatgg aagtaactca gttcacttac ttccaacaag ttggtggtgt tgaatgttac ccagtaacag gcgaaatcac ttacggttta gaacgtcttg caatgtacct tcaaggcgtt gactcggttt atgaccttgt ttggacgaaa ggccaattcg gtactgtaac ttacggcgac gtattccatc aaaatgaagt tgagcaatcg acttataact ttgaatacgc tccagttgac aaactatttg aactatttga tttctacgag tcagaagcta accgtttaat ggaagctaaa cttccacttc cagcttatga gcaagtcgtt aaagcgtctc atacctttaa cttgcttgat gcacgtggcg caatttctgt aactgagcgt caacgctaca ttttacgtgt acgtactttg gcgcgcgcta ttgcacaaag ttatgtacaa gcacgtgcag agcttggctt cccaatggca gaaccacact tacgtgatga agtattggca cagctaaaag cacaagctga aagtgaagca gcaaaggcgg agaaaaactg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat

Details for AcbaC.17993.a.B1.PW37692

PURIFICATION DATe:	4/7/2015
CONCENTRATION:	36mg/ml
OBSERVED MW:	35kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	18
VIAL VOLUME:	200µl
PERCENT IDENTITY:	93
PERCENT COVERAGE:	98

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

MAHHHHHHMS RAISHIDTFQ GLILALQNYW AEQGCVVLQP YDMEMGAGTF HTATFLHALG PETWNAAYVQ PSRRPKDGRY GENPNRLQHY YQFQVVLKPN PDNIQQLYLD SLKAIGIDTL THDIRFVEDN WESPTLGAWG LGWEVWLNGM EVTQFTYFQQ VGGVECYPVT GEITYGLERL AMYLQGVDSV YDLVWTKGQF GTVTYGDVFH QNEVEQSTYN FEYAPVDKLF ELFDFYESEA NRLMEAKLPL PAYEQVVKAS HTFNLLDARG AISVTERQRY ILRVRTLARA IAQSYVQARA ELGFPMAEPH LRDEVLAQLK AQAESEAAKA EKN

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGTC GCGCCATCTC GCACATTGAT ACATTTCAGG GCTTAATTCT TGCCCTACAA AACTATTGGG CCGAACAAGG CTGCGTCGTA CTACAACCTT ACGACATGGA AATGGGCGCT GGAACATTCC ACACCGCAAC ATTCTTACAT GCTTTAGGGC CAGAGACTTG GAACGCGGCA TACGTTCAAC CTTCACGTCG TCCAAAAGAT GGCCGCTACG GTGAAAACCC GAACCGTTTG CAACACTACT ATCAGTTCCA AGTGGTACTT AAGCCAAACC CAGACAACAT TCAACAGCTT TATCTCGATT CTTTAAAAGC AATCGGGATT GATACGCTTA CTCACGATAT CCGTTTCGTA GAAGACAACT GGGAATCTCC AACTCTTGGG GCTTGGGGCT TAGGTTGGGA AGTTTGGCTT AACGGTATGG AAGTAACTCA GTTCACTTAC TTCCAACAAG TTGGTGGTGT TGAATGTTAC CCAGTAACAG GCGAAATCAC TTACGGTTTA GAACGTCTTG CAATGTACCT TCAAGGCGTT GACTCGGTTT ATGACCTTGT TTGGACGAAA GGCCAATTCG GTACTGTAAC TTACGGCGAC GTATTCCATC AAAATGAAGT TGAGCAATCG ACTTATAACT TTGAATACGC TCCAGTTGAC AAACTATTTG AACTATTTGA TTTCTACGAG TCAGAAGCTA ACCGTTTAAT GGAAGCTAAA CTTCCACTTC CAGCTTATGA GCAAGTCGTT AAAGCGTCTC ATACCTTTAA CTTGCTTGAT GCACGTGGCG CAATTTCTGT AACTGAGCGT CAACGCTACA TTTTACGTGT ACGTACTTTG GCGCGCGCTA TTGCACAAAG TTATGTACAA GCACGTGCAG AGCTTGGCTT CCCAATGGCA GAACCACACT TACGTGATGA AGTATTGGCA CAGCTAAAAG CACAAGCTGA AAGTGAAGCA GCAAAGGCGG AGAAAAACTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT