AcbaC.17987.a

DNA polymerase III, beta subunit

CENTER ID: AcbaC.17987.a
ORGANISM: Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS: cloned
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AcbaC.17987.a.B1.GE38863 full length 1 382
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|470.1289.peg.13
GenBank: AKA29802.1
PGAT: acinetobacter:ABUW_0002
RefSeq: WP_001237350.1
UniProt: V5V7W3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
VRLKIAKESL LNVLSHVVGA VERRHTLNIL SNVKIQTNAQ ALTITGSDLE VELVASTALS EGACLEAGET TVPARKLMEI CKSLPTAALI DLQITEDQRC ILKSGNSRFV LGTLPAEDYP LLTTENSQGT QVQVTQRELK RLFEKTAFAM AVQDVRFYLT GTLLEIDENQ LRAVTTDGHR LALCEILASS TSSQLVQAIV PRKAVGELQR LLSIEDEQLT LLIGRELLNV TINTPSRDKE QGDITVRFTT KLIDGKFPDY RRVIPRGGDK HVLIGHDVFK QSLQRVAILS NEKLRGVFLN FNQDSLQLRA NNPEQDEAIE DLAIQYQSAP LEMSFNAQYL LDVLGVLDGD DVNMSMTEAN QSVLVQDPAH PDQTYVVMPM RV
NT Sequence
GTGCGTTTGA AAATCGCTAA AGAAAGTTTA CTCAATGTTT TATCGCATGT GGTCGGTGCG GTAGAACGTC GTCATACCTT GAATATTCTT TCAAACGTCA AAATTCAGAC TAATGCTCAA GCGCTCACTA TTACGGGTTC CGATTTAGAA GTTGAACTCG TAGCGAGCAC CGCTTTATCA GAAGGGGCTT GCCTTGAGGC AGGCGAGACA ACTGTTCCTG CTCGTAAATT GATGGAAATT TGTAAATCTT TGCCTACAGC TGCATTGATT GATTTACAAA TTACTGAAGA TCAAAGATGT ATTTTAAAGT CTGGTAATAG CCGTTTTGTG CTAGGTACAC TACCGGCTGA AGATTATCCA TTGTTAACCA CTGAGAATAG TCAAGGTACG CAAGTACAGG TGACTCAACG TGAATTAAAG CGTTTATTTG AAAAAACTGC TTTTGCAATG GCTGTACAAG ACGTACGTTT TTACCTCACT GGTACTTTAT TAGAAATTGA TGAAAATCAA TTGCGTGCAG TAACAACTGA TGGTCACCGT TTAGCACTTT GTGAAATTTT GGCTTCCTCT ACTTCATCTC AGTTGGTACA AGCGATTGTG CCTCGTAAAG CTGTGGGCGA ATTGCAACGT TTACTCAGCA TTGAAGATGA ACAGCTCACA TTGCTTATTG GCCGTGAATT ATTAAATGTA ACGATTAATA CGCCAAGCCG TGATAAAGAA CAAGGTGATA TTACAGTTCG TTTTACAACT AAATTGATTG ATGGGAAATT CCCTGATTAT CGCCGTGTTA TTCCACGTGG TGGTGATAAA CATGTTTTGA TTGGTCATGA CGTATTTAAA CAATCATTAC AACGTGTAGC AATTTTAAGT AATGAAAAAT TGCGTGGTGT TTTCCTAAAC TTCAATCAGG ATTCTTTACA GCTTCGTGCA AATAACCCTG AACAAGATGA AGCAATTGAA GATTTAGCGA TCCAATATCA AAGTGCCCCA TTAGAAATGT CATTTAATGC TCAATACCTA CTCGATGTGT TGGGTGTATT AGATGGTGAT GATGTAAATA TGAGCATGAC TGAGGCGAAT CAGTCTGTAT TGGTTCAAGA TCCGGCTCAT CCTGACCAGA CCTATGTAGT TATGCCGATG CGTGTTTAA
Details for AcbaC.17987.a.B1.GE38863
HARVESTED ON: data unavailable
SEQUENCED ON: 1/15/2015
EXPECTED MW: 43kDa
OBSERVED MW: 0kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: None
TOTAL EXPRESSION LEVEL: Data Unavailable
SOLUBLE EXPRESSION LEVEL Data Unavailable
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 96
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMV RLKIAKESLL NVLSHVVGAV ERRHTLNILS NVKIQTNAQA LTITGSDLEV ELVASTALSE GACLEAGETT VPARKLMEIC KSLPTAALID LQITEDQRCI LKSGNSRFVL GTLPAEDYPL LTTENSQGTQ VQVTQRELKR LFEKTAFAMA VQDVRFYLTG TLLEIDENQL RAVTTDGHRL ALCEILASST SSQLVQAIVP RKAVGELQRL LSIEDEQLTL LIGRELLNVT INTPSRDKEX XDIXXRFTTK LIDGKFPDYX XXIXXGGDKH VLIGHDVFXX SXQRVAILSN EKLRGVFLNF NQDSLQLRAN NPEQDEAIED LAIQYQSAPL EMSFNAQYLL DVLGVLDGDD VNMSMTEANQ SVLVQDPAHP DQXXXVMP
Validated NT Sequence
tcggcataac tannnnnnnc tggtcaggat gagccggatc ttgaaccaat acagactgat tcgcctcagt catgctcata tttacatcat caccatctaa tacacccaac acatcgagta ggtattgagc attaaatgac atttctaatg gggcactttg atattggatc gctaaatctt caattgcttc atcttgttca gggttatttg cacgaagctg taaagaatcc tgattgaagt ttaggaaaac accacgcaat ttttcattac ttaaaattgc tacacgttgt nnngatnnnn taaatacgtc atgaccaatc aaaacatgtt tatcaccacc angnngaata nnnnnnngat aatcagggaa tttcccatca atcaatttag ttgtaaaacg annnntaata tcannnngtt ctttatcacg gcttggcgta ttaatcgtta catttaataa ttcacggcca ataagcaatg tgagctgttc atcttcaatg ctgagtaaac gttgcaattc gcccacagct ttacgaggca caatcgcttg taccaactga gatgaagtag aggaagccaa aatttcacaa agtgctaaac ggtgaccatc agttgttact gcacgcaatt gattttcatc aatttctaat aaagtaccag tgaggtaaaa acgtacgtct tgtacagcca ttgcaaaagc agttttttca aataaacgct ttaattcacg ttgagtcacc tgtacttgcg taccttgact attctcagtg gttaacaatg gataatcttc agccggtagt gtacctagca caaaacggct attaccagac tttaaaatac atctttgatc ttcagtaatt tgtaaatcaa tcaatgcagc tgtaggcaaa gatttacaaa tttccatcaa tttacgagca ggaacagttg tctcgcctgc ctcaaggcaa gccccttctg ataaagcggt gctcgctacg agttcaactt ctaaatcgga acccgtaata gtgagcgctt gagcattagt ctgaattttg acgtttgaaa gaatattcaa ggtatgacga cgttctaccg caccgaccac atgcgataaa acattgagta aactttcttt agcgattttc aaacgcacca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHVR LKIAKESLLN VLSHVVGAVE RRHTLNILSN VKIQTNAQAL TITGSDLEVE LVASTALSEG ACLEAGETTV PARKLMEICK SLPTAALIDL QITEDQRCIL KSGNSRFVLG TLPAEDYPLL TTENSQGTQV QVTQRELKRL FEKTAFAMAV QDVRFYLTGT LLEIDENQLR AVTTDGHRLA LCEILASSTS SQLVQAIVPR KAVGELQRLL SIEDEQLTLL IGRELLNVTI NTPSRDKEQG DITVRFTTKL IDGKFPDYRR VIPRGGDKHV LIGHDVFKQS LQRVAILSNE KLRGVFLNFN QDSLQLRANN PEQDEAIEDL AIQYQSAPLE MSFNAQYLLD VLGVLDGDDV NMSMTEANQS VLVQDPAHPD QTYVVMPMRV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgcgtt tgaaaatcgc taaagaaagt ttactcaatg ttttatcgca tgtggtcggt gcggtagaac gtcgtcatac cttgaatatt ctttcaaacg tcaaaattca gactaatgct caagcgctca ctattacggg ttccgattta gaagttgaac tcgtagcgag caccgcttta tcagaagggg cttgccttga ggcaggcgag acaactgttc ctgctcgtaa attgatggaa atttgtaaat ctttgcctac agctgcattg attgatttac aaattactga agatcaaaga tgtattttaa agtctggtaa tagccgtttt gtgctaggta cactaccggc tgaagattat ccattgttaa ccactgagaa tagtcaaggt acgcaagtac aggtgactca acgtgaatta aagcgtttat ttgaaaaaac tgcttttgca atggctgtac aagacgtacg tttttacctc actggtactt tattagaaat tgatgaaaat caattgcgtg cagtaacaac tgatggtcac cgtttagcac tttgtgaaat tttggcttcc tctacttcat ctcagttggt acaagcgatt gtgcctcgta aagctgtggg cgaattgcaa cgtttactca gcattgaaga tgaacagctc acattgctta ttggccgtga attattaaat gtaacgatta atacgccaag ccgtgataaa gaacaaggtg atattacagt tcgttttaca actaaattga ttgatgggaa attccctgat tatcgccgtg ttattccacg tggtggtgat aaacatgttt tgattggtca tgacgtattt aaacaatcat tacaacgtgt agcaatttta agtaatgaaa aattgcgtgg tgttttccta aacttcaatc aggattcttt acagcttcgt gcaaataacc ctgaacaaga tgaagcaatt gaagatttag cgatccaata tcaaagtgcc ccattagaaa tgtcatttaa tgctcaatac ctactcgatg tgttgggtgt attagatggt gatgatgtaa atatgagcat gactgaggcg aatcagtctg tattggttca agatccggct catcctgacc agacctatgt agttatgccg atgcgtgttt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat