AcbaC.17981.a

prolyl-tRNA synthetase

CENTER ID:	AcbaC.17981.a
ORGANISM:	Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AcbaC.17981.a.B1.GE38861	full length		1	571

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AcbaC.17981.a.B1.PW37685	full length		1	571

External Resources

RESOURCE	REFERENCE ID
GenBank:	AKA30443.1
PATRIC ID:	fig\|470.1345.peg.644
PGAT:	acinetobacter:ABUW_0674
RefSeq:	WP_001202759.1
UniProt:	A0A0D5YEW0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRASRFLFAT LRETPNDAEV ISHQLMLRAG MIRKLASGLY TWLPMGTRVL KKVDAIVREE MNRSGAMEVF MPVTQPASLW EESGRYEQYG PELLRFKDRH DNPFVLGPTH EEVITDLARN ELKSYKQLPV NFYQIQTKFR DEIRPRFGVM RSREFIMKDA YSFHATQESL QETYDVMYDT YSRIFTRLGL DFRPVQADTG SIGGSASHEF HVLAASGEDD IAFSTESDYA ANVEMAEAVL VGERAAPTQE FKLVETPNQK TIADVCQFLN ADPKQSVKAL LVQGVADEKG NVPVVALFLR GDHELNEIKA EKHPLVAAPL AFATEEQLQA FGLTAGFTGP QGLVEKGITV IVDRAASVLS DFVAGANEAD KHAIGVNWER DAQITEVFDL RNVVEGDPSP DGKGTLQIKR GIEVGHIFQL GTKYSEALGC KVLGEDGKPF TVTMGCYGIG VTRVVAAAIE QNYDDKGIIW PQAIAPFEIA IVPMNAHKSP RTLEAAEALY AELQAQGFDV LLDDRNERPG VKFSDLELMG IPHRIVIGEK GLDAGTFEYK GRRDAEASNL TKEELLAKLA R

NT Sequence

ATGCGCGCAA GCCGCTTTTT ATTTGCAACG TTGAGAGAAA CCCCAAACGA TGCTGAAGTG ATTTCACATC AGCTCATGTT ACGTGCGGGG ATGATTCGTA AATTGGCATC TGGTTTATAT ACATGGCTGC CGATGGGTAC ACGTGTGCTT AAAAAAGTTG ATGCCATTGT TCGTGAAGAA ATGAACCGTT CAGGTGCTAT GGAAGTGTTC ATGCCCGTAA CTCAACCCGC AAGCTTGTGG GAAGAATCGG GTCGTTATGA ACAATATGGT CCTGAATTAT TACGTTTCAA AGATCGTCAC GACAATCCGT TTGTGCTTGG TCCTACACAT GAAGAAGTGA TTACCGATTT AGCACGTAAC GAGCTAAAAA GTTATAAACA GCTTCCTGTA AATTTCTATC AAATTCAAAC CAAATTCCGT GATGAGATCC GCCCTCGTTT TGGTGTAATG CGTTCACGCG AGTTCATCAT GAAAGATGCT TATTCTTTCC ATGCAACTCA AGAATCATTA CAAGAAACTT ATGATGTGAT GTATGACACC TATAGCCGTA TTTTCACTCG TTTAGGTTTA GATTTCCGCC CAGTACAAGC AGATACAGGT TCAATTGGTG GTTCAGCTTC ACATGAATTC CACGTATTAG CTGCAAGTGG TGAAGATGAC ATTGCATTTT CAACTGAGTC TGATTATGCA GCAAACGTTG AAATGGCTGA AGCTGTTTTA GTGGGTGAGC GTGCAGCGCC TACACAAGAA TTTAAATTGG TTGAAACACC AAATCAAAAA ACAATTGCTG ATGTTTGCCA GTTCTTAAAT GCTGACCCTA AACAATCTGT AAAAGCCTTA TTGGTTCAAG GCGTTGCTGA TGAGAAAGGC AATGTACCTG TCGTTGCCCT ATTCTTACGT GGTGACCACG AACTGAATGA AATTAAAGCC GAGAAACATC CTTTAGTGGC CGCTCCTCTC GCATTTGCCA CAGAAGAACA ACTTCAAGCA TTTGGCTTAA CTGCTGGCTT CACTGGTCCA CAAGGTTTAG TTGAAAAAGG CATCACTGTA ATTGTTGACC GTGCTGCATC TGTTCTTTCT GACTTCGTTG CTGGTGCAAA TGAGGCAGAC AAACATGCTA TAGGCGTAAA CTGGGAACGT GATGCACAAA TTACTGAAGT TTTCGATTTG CGTAATGTGG TTGAAGGTGA CCCATCTCCA GATGGTAAAG GTACACTACA AATTAAACGC GGTATTGAAG TGGGCCATAT TTTCCAACTT GGTACTAAAT ACTCTGAAGC TTTAGGATGT AAAGTTCTCG GTGAAGATGG CAAACCATTT ACAGTAACAA TGGGCTGTTA TGGTATTGGT GTGACACGCG TAGTTGCAGC TGCAATTGAA CAAAATTACG ATGACAAAGG GATTATTTGG CCACAAGCGA TTGCTCCTTT TGAAATCGCA ATTGTTCCAA TGAATGCACA CAAGTCACCA CGTACTCTTG AAGCAGCAGA AGCGCTTTAT GCAGAACTTC AAGCCCAAGG CTTTGACGTA TTACTTGATG ACCGTAACGA ACGTCCGGGC GTTAAATTCT CTGACCTTGA ACTAATGGGT ATTCCACACC GTATTGTGAT TGGTGAAAAA GGTCTTGATG CGGGTACTTT TGAATATAAA GGTCGCCGTG ATGCTGAAGC GAGCAATTTA ACTAAAGAAG AACTTTTAGC TAAATTAGCG CGTTAA

Details for AcbaC.17981.a.B1.GE38861

HARVESTED ON:	1/29/2015
SEQUENCED ON:	1/15/2015
EXPECTED MW:	64kDa
OBSERVED MW:	64kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMR ASRFLFATLR ETPNDAEVIS HQLMLRAGMI RKLASGLYTW LPMGTRVLKK VDAIVREEMN RSGAMEVFMP VTQPASLWEE SGRYEQYGPE LLRFKDRHDN PFVLGPTHEE VITDLARNEL KSYKQLPVNF YQIQTKFRDE IRPRFGVMRS REFIMKDAYS FHATQESLQE TYDVMYDTYS RIFTRLGLDF RPVQADTGSI GGSASHEFHV LAASGEDDIA FSTESDYAAN VEMAEAVLVG ERAAPTQEFK LVETPNQKTI ADVCQFLNAD PKQSVKALLV QGVADEKGNV PVVALFLRGD HELNEIKAEK HPLVAAPLAF ATEEQLQAFG LTAGFTGPQG LVEKGITVIV DRAASVLSDF VAGANEADKH AIGVNWERDA QITEVFDLRN VVEGDPSPDG KGTLQIKRGI EVGHIFQLGT KYSEALGCKV LGEDGKPFTV TMGCYGIGVT RVVAAAIEQN YDDKGIIWPQ AIAPFEIAIV PMNAHKSPRT LEAAEALYAE LQAQGFDVLL DDRNERPGVK FSDLELMGIP HRIVIGEKGL DAGTFEYKGR RDAEASNLTK EELLAKLAR

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgcg caagccgctt tttatttgca acgttgagag aaaccccaaa cgatgctgaa gtgatttcac atcagctcat gttacgtgcg gggatgattc gtaaattggc atctggttta tatacatggc tgccgatggg tacacgtgtg cttaaaaaag ttgatgccat tgttcgtgaa gaaatgaacc gttcaggtgc tatggaagtg ttcatgcccg taactcaacc cgcaagcttg tgggaagaat cgggtcgtta tgaacaatat ggtcctgaat tattacgttt caaagatcgt cacgacaatc cgtttgtgct tggtcctaca catgaagaag tgattaccga tttagcacgt aacgagctaa aaagttataa acagcttcct gtaaatttct atcaaattca aaccaaattc cgtgatgaga tccgccctcg ttttggtgta atgcgttcac gcgagttcat catgaaagat gcttattctt tccatgcaac tcaagaatca ttacaagaaa cttatgatgt gatgtatgac acctatagcc gtattttcac tcgtttaggt ttagatttcc gcccagtaca agcagataca ggttcaattg gtggttcagc ttcacatgaa ttccacgtat tagctgcaag tggtgaagat gacattgcat tttcaactga gtctgattat gcagcaaacg ttgaaatggc tgaagctgtt ttagtgggtg agcgtgcagc gcctacacaa gaatttaaat tggttgaaac accaaatcaa aaaacaattg ctgatgtttg ccagttctta aatgctgacc ctaaacaatc tgtaaaagcc ttattggttc aaggcgttgc tgatgagaaa ggcaatgtac ctgtcgttgc cctattctta cgtggtgacc acgaactgaa tgaaattaaa gccgagaaac atcctttagt ggccgctcct ctcgcatttg ccacagaaga acaacttcaa gcatttggct taactgctgg cttcactggt ccacaaggtt tagttgaaaa aggcatcact gtaattgttg accgtgctgc atctgttctt tctgacttcg ttgctggtgc aaatgaggca gacaaacatg ctataggcgt aaactgggaa cgtgatgcac aaattactga agttttcgat ttgcgtaatg tggttgaagg tgacccatct ccagatggta aaggtacact acaaattaaa cgcggtattg aagtgggcca tattttccaa cttggtacta aatactctga agctttagga tgtaaagttc tcggtgaaga tggcaaacca tttacagtaa caatgggctg ttatggtatt ggtgtgacac gcgtagttgc agctgcaatt gaacaaaatt acgatgacaa agggattatt tggccacaag cgattgctcc ttttgaaatc gcaattgttc caatgaatgc acacaagtca ccacgtactc ttgaagcagc agaagcgctt tatgcagaac ttcaagccca aggctttgac gtattacttg atgaccgtaa cgaacgtccg ggcgttaaat tctctgacct tgaactaatg ggtattccac accgtattgt gattggtgaa aaaggtcttg atgcgggtac ttttgaatat aaaggtcgcc gtgatgctga agcgagcaat ttaactaaag aagaactttt agctaaatta gcgcgttgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t

Details for AcbaC.17981.a.B1.PW37685

PURIFICATION DATe:	3/30/2015
CONCENTRATION:	15mg/ml
OBSERVED MW:	68kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	4
VIAL VOLUME:	200µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCGCG CAAGCCGCTT TTTATTTGCA ACGTTGAGAG AAACCCCAAA CGATGCTGAA GTGATTTCAC ATCAGCTCAT GTTACGTGCG GGGATGATTC GTAAATTGGC ATCTGGTTTA TATACATGGC TGCCGATGGG TACACGTGTG CTTAAAAAAG TTGATGCCAT TGTTCGTGAA GAAATGAACC GTTCAGGTGC TATGGAAGTG TTCATGCCCG TAACTCAACC CGCAAGCTTG TGGGAAGAAT CGGGTCGTTA TGAACAATAT GGTCCTGAAT TATTACGTTT CAAAGATCGT CACGACAATC CGTTTGTGCT TGGTCCTACA CATGAAGAAG TGATTACCGA TTTAGCACGT AACGAGCTAA AAAGTTATAA ACAGCTTCCT GTAAATTTCT ATCAAATTCA AACCAAATTC CGTGATGAGA TCCGCCCTCG TTTTGGTGTA ATGCGTTCAC GCGAGTTCAT CATGAAAGAT GCTTATTCTT TCCATGCAAC TCAAGAATCA TTACAAGAAA CTTATGATGT GATGTATGAC ACCTATAGCC GTATTTTCAC TCGTTTAGGT TTAGATTTCC GCCCAGTACA AGCAGATACA GGTTCAATTG GTGGTTCAGC TTCACATGAA TTCCACGTAT TAGCTGCAAG TGGTGAAGAT GACATTGCAT TTTCAACTGA GTCTGATTAT GCAGCAAACG TTGAAATGGC TGAAGCTGTT TTAGTGGGTG AGCGTGCAGC GCCTACACAA GAATTTAAAT TGGTTGAAAC ACCAAATCAA AAAACAATTG CTGATGTTTG CCAGTTCTTA AATGCTGACC CTAAACAATC TGTAAAAGCC TTATTGGTTC AAGGCGTTGC TGATGAGAAA GGCAATGTAC CTGTCGTTGC CCTATTCTTA CGTGGTGACC ACGAACTGAA TGAAATTAAA GCCGAGAAAC ATCCTTTAGT GGCCGCTCCT CTCGCATTTG CCACAGAAGA ACAACTTCAA GCATTTGGCT TAACTGCTGG CTTCACTGGT CCACAAGGTT TAGTTGAAAA AGGCATCACT GTAATTGTTG ACCGTGCTGC ATCTGTTCTT TCTGACTTCG TTGCTGGTGC AAATGAGGCA GACAAACATG CTATAGGCGT AAACTGGGAA CGTGATGCAC AAATTACTGA AGTTTTCGAT TTGCGTAATG TGGTTGAAGG TGACCCATCT CCAGATGGTA AAGGTACACT ACAAATTAAA CGCGGTATTG AAGTGGGCCA TATTTTCCAA CTTGGTACTA AATACTCTGA AGCTTTAGGA TGTAAAGTTC TCGGTGAAGA TGGCAAACCA TTTACAGTAA CAATGGGCTG TTATGGTATT GGTGTGACAC GCGTAGTTGC AGCTGCAATT GAACAAAATT ACGATGACAA AGGGATTATT TGGCCACAAG CGATTGCTCC TTTTGAAATC GCAATTGTTC CAATGAATGC ACACAAGTCA CCACGTACTC TTGAAGCAGC AGAAGCGCTT TATGCAGAAC TTCAAGCCCA AGGCTTTGAC GTATTACTTG ATGACCGTAA CGAACGTCCG GGCGTTAAAT TCTCTGACCT TGAACTAATG GGTATTCCAC ACCGTATTGT GATTGGTGAA AAAGGTCTTG ATGCGGGTAC TTTTGAATAT AAAGGTCGCC GTGATGCTGA AGCGAGCAAT TTAACTAAAG AAGAACTTTT AGCTAAATTA GCGCGTTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T