AcbaC.17974.a

preprotein translocase, SecA subunit

CENTER ID: AcbaC.17974.a
ORGANISM: Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AcbaC.17974.a.B1.GE38858 full length 1 907
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AcbaC.17974.a.B1.PS02396 full length 1 907

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|470.1345.peg.599
GenBank: AKA30399.1
PGAT: acinetobacter:ABUW_0629
RefSeq: WP_000881342.1
UniProt: A0A0D8GEK5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLASLIGGIF GTKNERELKR MRKIVEQINA LEPTISALSD ADLSAKTPEF KQRYNNGESL DKLLPEAFAV CREAAKRVMG MRHYDVQLIG GITLHEGKIA EMRTGEGKTL MGTLACYLNA LSGEGVHVIT VNDYLAQRDA ELNRPLFEFL GLSIGTIYSM QEPAEKAAAY LADITYGTNN EFGFDYLRDN MVFSLAEKKQ RGLHYAIIDE VDSILIDEAR TPLIISGQSE DSSHLYTAIN TIPPKLRPQK EEKVADGGHF WIDEKQRSVE MTEIGYETVE QELIQMGLLA EGESLYSATN LNLVHHVSAA IRAHFLFQRD VHYIIHDGEV IIVDEHTGRT MPGRRWSEGL HQAVEAKEGL AIQPENQTLA TTTFQNYFRL YKKLSGMTGT ADTEAAEMKE IYGLDVVIIP THRPMIRNDQ NDLIYLNRNG KYNAIIQEIM NIRQQGVAPI LIGTATIEAS EILSSKLKQA GIHHEVLNAK QHEREADIIA QAGSPNAVTI ATNMAGRGTD IILGGNWKAK LAKLENPTPE DEARLKAQWE QDHEDVLQAG GLHIIGSERH ESRRIDNQLR GRAGRQGDPG VSRFYLSLED DLMRIFAGDR VVAMMRAMGL KEDEAIEHKM VSRSIENAQR KVEARNFDIR KNLLKYDDVN NEQRKIIYSQ RDEILAENTL QEYVEEMHRE VMQAMIANFI PPESIHDQWD VEGLENALRI DLGIELPVQE WLEQDRRLDE EGLVERISDE VIARYRQRRA QMGDESAAML ERHFVLNSLD RHWKDHLAAM DYLRQGIHLR GYAQKNPEQE YKKEAFNLFV NMLGVIKTDV VTDLSRVHIP TPEELAEMEA QQQQQAEAMK LSFEHDDVDG LTGEVTASQE ALNDSATEQQ TFPVPESRNA PCPCGSGLKY KQCHGKI
NT Sequence
ATGTTGGCAA GTCTGATCGG AGGTATCTTC GGTACAAAAA ATGAGCGTGA ACTCAAACGC ATGCGCAAAA TTGTTGAGCA AATCAATGCG CTCGAGCCGA CGATATCAGC TTTAAGCGAT GCAGACTTAT CTGCAAAAAC TCCAGAATTC AAACAACGTT ATAACAATGG CGAAAGTTTA GATAAGTTAC TCCCTGAGGC GTTTGCAGTT TGTCGTGAGG CAGCAAAACG TGTAATGGGC ATGCGCCACT ACGATGTCCA GTTAATTGGT GGTATTACCT TACATGAAGG CAAAATTGCC GAAATGCGTA CCGGTGAAGG TAAAACCCTT ATGGGTACTT TAGCGTGTTA CTTAAATGCA TTAAGTGGTG AAGGCGTTCA TGTCATTACT GTAAACGATT ACTTGGCACA ACGTGATGCT GAGTTAAACC GCCCTTTATT TGAATTTTTA GGTCTAAGCA TTGGTACAAT TTATTCAATG CAAGAACCAG CAGAAAAAGC AGCTGCCTAT CTTGCTGATA TTACCTATGG TACTAATAAC GAATTTGGTT TTGACTACTT GCGTGACAAC ATGGTGTTTT CTTTAGCAGA AAAGAAACAA CGTGGTTTGC ACTATGCCAT TATCGATGAA GTCGACTCAA TCTTGATTGA TGAGGCGCGT ACGCCATTAA TTATTTCTGG TCAAAGTGAA GATTCTTCAC ACCTCTATAC TGCCATCAAT ACGATTCCAC CAAAATTACG CCCGCAAAAA GAAGAAAAAG TTGCCGATGG CGGACATTTC TGGATTGATG AAAAGCAGCG TTCAGTTGAA ATGACTGAAA TTGGTTATGA AACAGTAGAA CAAGAACTTA TTCAAATGGG TTTGTTAGCC GAAGGCGAAA GCTTATATTC AGCTACAAAT CTAAACCTCG TTCACCATGT TTCTGCGGCT ATTCGTGCGC ACTTCCTATT CCAACGTGAC GTTCACTATA TTATTCATGA CGGTGAAGTC ATTATTGTTG ATGAGCATAC TGGCCGTACC ATGCCGGGCC GTCGTTGGTC AGAAGGCTTA CACCAAGCAG TTGAAGCGAA AGAAGGTTTA GCTATTCAAC CCGAAAACCA GACTTTAGCA ACAACTACTT TCCAGAACTA TTTCCGTCTT TATAAAAAGC TTTCAGGTAT GACAGGTACT GCCGATACTG AAGCTGCGGA AATGAAAGAA ATTTATGGTC TGGACGTCGT TATTATTCCG ACACACCGAC CTATGATTCG TAATGACCAG AACGATTTAA TCTATTTAAA CCGTAATGGT AAATATAACG CGATTATTCA AGAAATTATG AATATTCGTC AGCAGGGTGT TGCACCGATT CTTATTGGTA CGGCAACGAT TGAAGCCAGT GAAATTCTAT CGTCTAAATT GAAGCAAGCT GGTATTCATC ATGAAGTTTT GAACGCAAAA CAACATGAGC GTGAAGCAGA CATTATTGCT CAGGCAGGTA GCCCGAATGC AGTAACAATC GCAACAAACA TGGCCGGTCG TGGTACAGAT ATTATCCTTG GGGGTAACTG GAAAGCGAAA CTTGCCAAAC TTGAAAATCC TACCCCTGAA GATGAAGCGC GTCTAAAAGC GCAATGGGAA CAGGACCATG AAGATGTATT GCAAGCAGGT GGCTTGCACA TTATTGGTTC TGAACGCCAT GAATCACGCC GTATTGACAA CCAGTTACGT GGACGTGCGG GCCGTCAGGG TGACCCTGGT GTTTCTCGTT TCTATTTATC TCTTGAAGAT GACCTCATGC GTATTTTCGC AGGCGATCGT GTCGTTGCCA TGATGCGTGC AATGGGGCTT AAAGAAGATG AAGCGATTGA ACACAAAATG GTAAGCCGTT CTATCGAAAA CGCGCAGCGT AAAGTTGAAG CACGTAACTT CGATATTCGT AAAAACTTAC TTAAATATGA TGATGTGAAT AATGAACAAC GTAAGATCAT TTATTCACAA CGCGACGAAA TCTTAGCTGA AAATACTTTA CAAGAATACG TTGAAGAAAT GCATCGTGAA GTAATGCAGG CGATGATTGC TAACTTTATT CCACCAGAGT CAATCCATGA CCAATGGGAT GTGGAAGGCT TAGAAAATGC TTTACGTATT GATTTGGGTA TTGAGCTACC AGTTCAAGAA TGGCTAGAAC AAGATCGACG TCTTGATGAA GAAGGCTTGG TTGAACGCAT CAGTGATGAA GTAATTGCGC GTTATCGTCA GCGCCGTGCA CAAATGGGTG ATGAATCGGC AGCAATGCTT GAGCGTCATT TCGTTTTAAA TTCTCTTGAT CGCCATTGGA AAGATCATTT GGCTGCAATG GATTATTTAC GTCAAGGTAT CCACTTACGT GGCTATGCGC AAAAAAATCC TGAGCAGGAA TATAAAAAAG AAGCATTTAA CTTATTCGTG AACATGCTGG GTGTAATTAA AACTGATGTT GTAACTGATC TGTCTCGCGT ACATATTCCA ACACCAGAAG AGCTTGCAGA AATGGAAGCT CAACAGCAGC AACAAGCAGA AGCAATGAAG CTTTCTTTTG AACATGATGA TGTGGATGGA TTAACAGGTG AAGTGACCGC TTCTCAAGAA GCACTTAATG ATTCGGCTAC TGAACAACAA ACGTTCCCTG TACCTGAAAG TCGTAATGCT CCATGTCCAT GTGGTTCTGG TTTGAAATAT AAACAGTGTC ACGGCAAAAT TTAA
Details for AcbaC.17974.a.B1.GE38858
HARVESTED ON: 1/29/2015
SEQUENCED ON: 1/15/2015
EXPECTED MW: 104kDa
OBSERVED MW: 104kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 94
PERCENT COVERAGE: 33
Validated AA Sequence
MAHHHHHHML ASLIGGIFGT KNERELKRMR KIVEQINALE PTISALSDAD LSAKTPEFKQ RYNNGESLDK LLPEAFAVCR EAAKRVMGMR HYDVQLIGGI TLHEGKIAEM RTGEGKTLMG TLACYLNALS GEGVHVITVN DYLAQRDAEL NRPLFEFLGL SIGTIYSMQE PAEKAAAYLA DITYGTNNEF GFDYLRDNMV FSLAEKKQRG LHYAIIDEVD SILIDEARTP LIISGQSEDS SHLYTAINTI PPKLRPQKXX KVADGGHFWI DEKQRSVEMT XXGXXXVXQE LIQMGXLAXX RXLIFSXXSK
Validated NT Sequence
atggctcacc accaccacca ccatatgttg gcaagtctga tcggaggtat cttcggtaca aaaaatgagc gtgaactcaa acgcatgcgc aaaattgttg agcaaatcaa tgcgctcgag ccgacgatat cagctttaag cgatgcagac ttatctgcaa aaactccaga attcaaacaa cgttataaca atggcgaaag tttagataag ttactccctg aggcgtttgc agtttgtcgt gaggcagcaa aacgtgtaat gggcatgcgc cactacgatg tccagttaat tggtggtatt accttacatg aaggcaaaat tgccgaaatg cgtaccggtg aaggtaaaac ccttatgggt actttagcgt gttacttaaa tgcattaagt ggtgaaggcg ttcatgtcat tactgtaaac gattacttgg cacaacgtga tgctgagtta aaccgccctt tatttgaatt tttaggtcta agcattggta caatttattc aatgcaagaa ccagcagaaa aagcagctgc ctatcttgct gatattacct atggtactaa taacgaattt ggttttgact acttgcgtga caacatggtg ttttctttag cagaaaagaa acaacgtggt ttgcactatg ccattatcga tgaagtcgac tcaatcttga ttgatgaggc gcgtacgcca ttaattattt ctggtcaaag tgaagattct tcacacctct atactgccat caatacgatt ccaccaaaat tacgcccgca aaaagannna aaagttgccg atggcggaca tttctggatt gatgaaaagc agcgttcagt ngaaatgact nnnnntggtt annnnncagt annacaagaa cttattcaaa tgggttngtt agcnnnnnng cgnangctta tattcagctn nnnntctaaa c
Expected Protein Sequence
MAHHHHHHML ASLIGGIFGT KNERELKRMR KIVEQINALE PTISALSDAD LSAKTPEFKQ RYNNGESLDK LLPEAFAVCR EAAKRVMGMR HYDVQLIGGI TLHEGKIAEM RTGEGKTLMG TLACYLNALS GEGVHVITVN DYLAQRDAEL NRPLFEFLGL SIGTIYSMQE PAEKAAAYLA DITYGTNNEF GFDYLRDNMV FSLAEKKQRG LHYAIIDEVD SILIDEARTP LIISGQSEDS SHLYTAINTI PPKLRPQKEE KVADGGHFWI DEKQRSVEMT EIGYETVEQE LIQMGLLAEG ESLYSATNLN LVHHVSAAIR AHFLFQRDVH YIIHDGEVII VDEHTGRTMP GRRWSEGLHQ AVEAKEGLAI QPENQTLATT TFQNYFRLYK KLSGMTGTAD TEAAEMKEIY GLDVVIIPTH RPMIRNDQND LIYLNRNGKY NAIIQEIMNI RQQGVAPILI GTATIEASEI LSSKLKQAGI HHEVLNAKQH EREADIIAQA GSPNAVTIAT NMAGRGTDII LGGNWKAKLA KLENPTPEDE ARLKAQWEQD HEDVLQAGGL HIIGSERHES RRIDNQLRGR AGRQGDPGVS RFYLSLEDDL MRIFAGDRVV AMMRAMGLKE DEAIEHKMVS RSIENAQRKV EARNFDIRKN LLKYDDVNNE QRKIIYSQRD EILAENTLQE YVEEMHREVM QAMIANFIPP ESIHDQWDVE GLENALRIDL GIELPVQEWL EQDRRLDEEG LVERISDEVI ARYRQRRAQM GDESAAMLER HFVLNSLDRH WKDHLAAMDY LRQGIHLRGY AQKNPEQEYK KEAFNLFVNM LGVIKTDVVT DLSRVHIPTP EELAEMEAQQ QQQAEAMKLS FEHDDVDGLT GEVTASQEAL NDSATEQQTF PVPESRNAPC PCGSGLKYKQ CHGKI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttgg caagtctgat cggaggtatc ttcggtacaa aaaatgagcg tgaactcaaa cgcatgcgca aaattgttga gcaaatcaat gcgctcgagc cgacgatatc agctttaagc gatgcagact tatctgcaaa aactccagaa ttcaaacaac gttataacaa tggcgaaagt ttagataagt tactccctga ggcgtttgca gtttgtcgtg aggcagcaaa acgtgtaatg ggcatgcgcc actacgatgt ccagttaatt ggtggtatta ccttacatga aggcaaaatt gccgaaatgc gtaccggtga aggtaaaacc cttatgggta ctttagcgtg ttacttaaat gcattaagtg gtgaaggcgt tcatgtcatt actgtaaacg attacttggc acaacgtgat gctgagttaa accgcccttt atttgaattt ttaggtctaa gcattggtac aatttattca atgcaagaac cagcagaaaa agcagctgcc tatcttgctg atattaccta tggtactaat aacgaatttg gttttgacta cttgcgtgac aacatggtgt tttctttagc agaaaagaaa caacgtggtt tgcactatgc cattatcgat gaagtcgact caatcttgat tgatgaggcg cgtacgccat taattatttc tggtcaaagt gaagattctt cacacctcta tactgccatc aatacgattc caccaaaatt acgcccgcaa aaagaagaaa aagttgccga tggcggacat ttctggattg atgaaaagca gcgttcagtt gaaatgactg aaattggtta tgaaacagta gaacaagaac ttattcaaat gggtttgtta gccgaaggcg aaagcttata ttcagctaca aatctaaacc tcgttcacca tgtttctgcg gctattcgtg cgcacttcct attccaacgt gacgttcact atattattca tgacggtgaa gtcattattg ttgatgagca tactggccgt accatgccgg gccgtcgttg gtcagaaggc ttacaccaag cagttgaagc gaaagaaggt ttagctattc aacccgaaaa ccagacttta gcaacaacta ctttccagaa ctatttccgt ctttataaaa agctttcagg tatgacaggt actgccgata ctgaagctgc ggaaatgaaa gaaatttatg gtctggacgt cgttattatt ccgacacacc gacctatgat tcgtaatgac cagaacgatt taatctattt aaaccgtaat ggtaaatata acgcgattat tcaagaaatt atgaatattc gtcagcaggg tgttgcaccg attcttattg gtacggcaac gattgaagcc agtgaaattc tatcgtctaa attgaagcaa gctggtattc atcatgaagt tttgaacgca aaacaacatg agcgtgaagc agacattatt gctcaggcag gtagcccgaa tgcagtaaca atcgcaacaa acatggccgg tcgtggtaca gatattatcc ttgggggtaa ctggaaagcg aaacttgcca aacttgaaaa tcctacccct gaagatgaag cgcgtctaaa agcgcaatgg gaacaggacc atgaagatgt attgcaagca ggtggcttgc acattattgg ttctgaacgc catgaatcac gccgtattga caaccagtta cgtggacgtg cgggccgtca gggtgaccct ggtgtttctc gtttctattt atctcttgaa gatgacctca tgcgtatttt cgcaggcgat cgtgtcgttg ccatgatgcg tgcaatgggg cttaaagaag atgaagcgat tgaacacaaa atggtaagcc gttctatcga aaacgcgcag cgtaaagttg aagcacgtaa cttcgatatt cgtaaaaact tacttaaata tgatgatgtg aataatgaac aacgtaagat catttattca caacgcgacg aaatcttagc tgaaaatact ttacaagaat acgttgaaga aatgcatcgt gaagtaatgc aggcgatgat tgctaacttt attccaccag agtcaatcca tgaccaatgg gatgtggaag gcttagaaaa tgctttacgt attgatttgg gtattgagct accagttcaa gaatggctag aacaagatcg acgtcttgat gaagaaggct tggttgaacg catcagtgat gaagtaattg cgcgttatcg tcagcgccgt gcacaaatgg gtgatgaatc ggcagcaatg cttgagcgtc atttcgtttt aaattctctt gatcgccatt ggaaagatca tttggctgca atggattatt tacgtcaagg tatccactta cgtggctatg cgcaaaaaaa tcctgagcag gaatataaaa aagaagcatt taacttattc gtgaacatgc tgggtgtaat taaaactgat gttgtaactg atctgtctcg cgtacatatt ccaacaccag aagagcttgc agaaatggaa gctcaacagc agcaacaagc agaagcaatg aagctttctt ttgaacatga tgatgtggat ggattaacag gtgaagtgac cgcttctcaa gaagcactta atgattcggc tactgaacaa caaacgttcc ctgtacctga aagtcgtaat gctccatgtc catgtggttc tggtttgaaa tataaacagt gtcacggcaa aatttgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for AcbaC.17974.a.B1.PS02396
PURIFICATION DATe: 5/5/2015
CONCENTRATION: 45.14mg/ml
OBSERVED MW: 110kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 1
VIAL VOLUME: 110µl
PERCENT IDENTITY: 94
PERCENT COVERAGE: 33
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHML ASLIGGIFGT KNERELKRMR KIVEQINALE PTISALSDAD LSAKTPEFKQ RYNNGESLDK LLPEAFAVCR EAAKRVMGMR HYDVQLIGGI TLHEGKIAEM RTGEGKTLMG TLACYLNALS GEGVHVITVN DYLAQRDAEL NRPLFEFLGL SIGTIYSMQE PAEKAAAYLA DITYGTNNEF GFDYLRDNMV FSLAEKKQRG LHYAIIDEVD SILIDEARTP LIISGQSEDS SHLYTAINTI PPKLRPQKXX KVADGGHFWI DEKQRSVEMT XXGXXXVXQE LIQMGXLAXX RXLIFSXXSK
Validated NT Sequence
atggctcacc accaccacca ccatatgttg gcaagtctga tcggaggtat cttcggtaca aaaaatgagc gtgaactcaa acgcatgcgc aaaattgttg agcaaatcaa tgcgctcgag ccgacgatat cagctttaag cgatgcagac ttatctgcaa aaactccaga attcaaacaa cgttataaca atggcgaaag tttagataag ttactccctg aggcgtttgc agtttgtcgt gaggcagcaa aacgtgtaat gggcatgcgc cactacgatg tccagttaat tggtggtatt accttacatg aaggcaaaat tgccgaaatg cgtaccggtg aaggtaaaac ccttatgggt actttagcgt gttacttaaa tgcattaagt ggtgaaggcg ttcatgtcat tactgtaaac gattacttgg cacaacgtga tgctgagtta aaccgccctt tatttgaatt tttaggtcta agcattggta caatttattc aatgcaagaa ccagcagaaa aagcagctgc ctatcttgct gatattacct atggtactaa taacgaattt ggttttgact acttgcgtga caacatggtg ttttctttag cagaaaagaa acaacgtggt ttgcactatg ccattatcga tgaagtcgac tcaatcttga ttgatgaggc gcgtacgcca ttaattattt ctggtcaaag tgaagattct tcacacctct atactgccat caatacgatt ccaccaaaat tacgcccgca aaaagannna aaagttgccg atggcggaca tttctggatt gatgaaaagc agcgttcagt ngaaatgact nnnnntggtt annnnncagt annacaagaa cttattcaaa tgggttngtt agcnnnnnng cgnangctta tattcagctn nnnntctaaa c
Expressed Protein Sequence
MAHHHHHHML ASLIGGIFGT KNERELKRMR KIVEQINALE PTISALSDAD LSAKTPEFKQ RYNNGESLDK LLPEAFAVCR EAAKRVMGMR HYDVQLIGGI TLHEGKIAEM RTGEGKTLMG TLACYLNALS GEGVHVITVN DYLAQRDAEL NRPLFEFLGL SIGTIYSMQE PAEKAAAYLA DITYGTNNEF GFDYLRDNMV FSLAEKKQRG LHYAIIDEVD SILIDEARTP LIISGQSEDS SHLYTAINTI PPKLRPQKEE KVADGGHFWI DEKQRSVEMT EIGYETVEQE LIQMGLLAEG ESLYSATNLN LVHHVSAAIR AHFLFQRDVH YIIHDGEVII VDEHTGRTMP GRRWSEGLHQ AVEAKEGLAI QPENQTLATT TFQNYFRLYK KLSGMTGTAD TEAAEMKEIY GLDVVIIPTH RPMIRNDQND LIYLNRNGKY NAIIQEIMNI RQQGVAPILI GTATIEASEI LSSKLKQAGI HHEVLNAKQH EREADIIAQA GSPNAVTIAT NMAGRGTDII LGGNWKAKLA KLENPTPEDE ARLKAQWEQD HEDVLQAGGL HIIGSERHES RRIDNQLRGR AGRQGDPGVS RFYLSLEDDL MRIFAGDRVV AMMRAMGLKE DEAIEHKMVS RSIENAQRKV EARNFDIRKN LLKYDDVNNE QRKIIYSQRD EILAENTLQE YVEEMHREVM QAMIANFIPP ESIHDQWDVE GLENALRIDL GIELPVQEWL EQDRRLDEEG LVERISDEVI ARYRQRRAQM GDESAAMLER HFVLNSLDRH WKDHLAAMDY LRQGIHLRGY AQKNPEQEYK KEAFNLFVNM LGVIKTDVVT DLSRVHIPTP EELAEMEAQQ QQQAEAMKLS FEHDDVDGLT GEVTASQEAL NDSATEQQTF PVPESRNAPC PCGSGLKYKQ CHGKI
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTTGG CAAGTCTGAT CGGAGGTATC TTCGGTACAA AAAATGAGCG TGAACTCAAA CGCATGCGCA AAATTGTTGA GCAAATCAAT GCGCTCGAGC CGACGATATC AGCTTTAAGC GATGCAGACT TATCTGCAAA AACTCCAGAA TTCAAACAAC GTTATAACAA TGGCGAAAGT TTAGATAAGT TACTCCCTGA GGCGTTTGCA GTTTGTCGTG AGGCAGCAAA ACGTGTAATG GGCATGCGCC ACTACGATGT CCAGTTAATT GGTGGTATTA CCTTACATGA AGGCAAAATT GCCGAAATGC GTACCGGTGA AGGTAAAACC CTTATGGGTA CTTTAGCGTG TTACTTAAAT GCATTAAGTG GTGAAGGCGT TCATGTCATT ACTGTAAACG ATTACTTGGC ACAACGTGAT GCTGAGTTAA ACCGCCCTTT ATTTGAATTT TTAGGTCTAA GCATTGGTAC AATTTATTCA ATGCAAGAAC CAGCAGAAAA AGCAGCTGCC TATCTTGCTG ATATTACCTA TGGTACTAAT AACGAATTTG GTTTTGACTA CTTGCGTGAC AACATGGTGT TTTCTTTAGC AGAAAAGAAA CAACGTGGTT TGCACTATGC CATTATCGAT GAAGTCGACT CAATCTTGAT TGATGAGGCG CGTACGCCAT TAATTATTTC TGGTCAAAGT GAAGATTCTT CACACCTCTA TACTGCCATC AATACGATTC CACCAAAATT ACGCCCGCAA AAAGAAGAAA AAGTTGCCGA TGGCGGACAT TTCTGGATTG ATGAAAAGCA GCGTTCAGTT GAAATGACTG AAATTGGTTA TGAAACAGTA GAACAAGAAC TTATTCAAAT GGGTTTGTTA GCCGAAGGCG AAAGCTTATA TTCAGCTACA AATCTAAACC TCGTTCACCA TGTTTCTGCG GCTATTCGTG CGCACTTCCT ATTCCAACGT GACGTTCACT ATATTATTCA TGACGGTGAA GTCATTATTG TTGATGAGCA TACTGGCCGT ACCATGCCGG GCCGTCGTTG GTCAGAAGGC TTACACCAAG CAGTTGAAGC GAAAGAAGGT TTAGCTATTC AACCCGAAAA CCAGACTTTA GCAACAACTA CTTTCCAGAA CTATTTCCGT CTTTATAAAA AGCTTTCAGG TATGACAGGT ACTGCCGATA CTGAAGCTGC GGAAATGAAA GAAATTTATG GTCTGGACGT CGTTATTATT CCGACACACC GACCTATGAT TCGTAATGAC CAGAACGATT TAATCTATTT AAACCGTAAT GGTAAATATA ACGCGATTAT TCAAGAAATT ATGAATATTC GTCAGCAGGG TGTTGCACCG ATTCTTATTG GTACGGCAAC GATTGAAGCC AGTGAAATTC TATCGTCTAA ATTGAAGCAA GCTGGTATTC ATCATGAAGT TTTGAACGCA AAACAACATG AGCGTGAAGC AGACATTATT GCTCAGGCAG GTAGCCCGAA TGCAGTAACA ATCGCAACAA ACATGGCCGG TCGTGGTACA GATATTATCC TTGGGGGTAA CTGGAAAGCG AAACTTGCCA AACTTGAAAA TCCTACCCCT GAAGATGAAG CGCGTCTAAA AGCGCAATGG GAACAGGACC ATGAAGATGT ATTGCAAGCA GGTGGCTTGC ACATTATTGG TTCTGAACGC CATGAATCAC GCCGTATTGA CAACCAGTTA CGTGGACGTG CGGGCCGTCA GGGTGACCCT GGTGTTTCTC GTTTCTATTT ATCTCTTGAA GATGACCTCA TGCGTATTTT CGCAGGCGAT CGTGTCGTTG CCATGATGCG TGCAATGGGG CTTAAAGAAG ATGAAGCGAT TGAACACAAA ATGGTAAGCC GTTCTATCGA AAACGCGCAG CGTAAAGTTG AAGCACGTAA CTTCGATATT CGTAAAAACT TACTTAAATA TGATGATGTG AATAATGAAC AACGTAAGAT CATTTATTCA CAACGCGACG AAATCTTAGC TGAAAATACT TTACAAGAAT ACGTTGAAGA AATGCATCGT GAAGTAATGC AGGCGATGAT TGCTAACTTT ATTCCACCAG AGTCAATCCA TGACCAATGG GATGTGGAAG GCTTAGAAAA TGCTTTACGT ATTGATTTGG GTATTGAGCT ACCAGTTCAA GAATGGCTAG AACAAGATCG ACGTCTTGAT GAAGAAGGCT TGGTTGAACG CATCAGTGAT GAAGTAATTG CGCGTTATCG TCAGCGCCGT GCACAAATGG GTGATGAATC GGCAGCAATG CTTGAGCGTC ATTTCGTTTT AAATTCTCTT GATCGCCATT GGAAAGATCA TTTGGCTGCA ATGGATTATT TACGTCAAGG TATCCACTTA CGTGGCTATG CGCAAAAAAA TCCTGAGCAG GAATATAAAA AAGAAGCATT TAACTTATTC GTGAACATGC TGGGTGTAAT TAAAACTGAT GTTGTAACTG ATCTGTCTCG CGTACATATT CCAACACCAG AAGAGCTTGC AGAAATGGAA GCTCAACAGC AGCAACAAGC AGAAGCAATG AAGCTTTCTT TTGAACATGA TGATGTGGAT GGATTAACAG GTGAAGTGAC CGCTTCTCAA GAAGCACTTA ATGATTCGGC TACTGAACAA CAAACGTTCC CTGTACCTGA AAGTCGTAAT GCTCCATGTC CATGTGGTTC TGGTTTGAAA TATAAACAGT GTCACGGCAA AATTTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT