SSGCID: Target Page

AcbaC.17966.a

succinate dehydrogenase iron-sulfur subunit

CENTER ID:	AcbaC.17966.a
ORGANISM:	Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS:	cloned
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AcbaC.17966.a.B1.GE38855	full length		1	236

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|470.1345.peg.838
GenBank:	AKA30631.1
PGAT:	acinetobacter:ABUW_0871
RefSeq:	WP_000086385.1
UniProt:	V5VAD3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSRGTRTFEI YRYDPDKDKA PYMQTFKLEL TDKHRMLLDA LLALKVQDET LTFRRSCREG ICGSDGVNIN GKNGLACLQN LNDLPEKIVI RPLPGLPVVK DLVVDMNQFY DQYDKIQPFL INNQPAPPKE RLQSPEEREH LNGLYECILC ACCSTSCPSF WWNPDKFLGP SALLNAYRFI IDSRDTATAE RLARLDDPFS LFRCKGIMNC VSVCPKGLNP TKAIGHIRNM LLDQAG

NT Sequence

ATGAGTAGAG GTACTCGTAC ATTCGAAATC TACCGCTATG ATCCTGATAA GGATAAAGCG CCGTACATGC AAACTTTCAA GCTTGAATTG ACTGATAAGC ACCGTATGTT GCTTGATGCA TTGCTTGCTT TGAAGGTTCA AGACGAAACA TTAACATTCC GTCGTTCTTG CCGTGAAGGT ATTTGTGGTT CTGATGGTGT GAATATCAAT GGTAAAAATG GTTTGGCTTG CTTGCAAAAC CTTAACGATT TACCAGAGAA AATTGTAATT CGTCCATTAC CTGGTTTGCC GGTTGTTAAA GATTTGGTTG TTGATATGAA CCAATTCTAT GATCAGTATG ACAAAATTCA GCCATTCTTG ATCAATAACC AACCTGCTCC ACCTAAAGAG CGTTTGCAAT CTCCTGAAGA GCGTGAACAC TTAAATGGTT TGTATGAGTG TATTCTTTGT GCATGTTGTT CAACTTCATG CCCATCATTC TGGTGGAACC CTGATAAATT CTTGGGTCCT TCAGCGTTGT TGAATGCATA TCGTTTTATT ATCGACTCTC GTGATACTGC AACTGCAGAA CGTTTGGCTC GTCTTGACGA TCCGTTCAGT TTGTTCCGTT GTAAAGGTAT TATGAATTGT GTGTCAGTAT GCCCTAAAGG TTTGAATCCA ACAAAAGCAA TCGGTCATAT CCGTAATATG TTGTTAGATC AAGCGGGTTA A

Details for AcbaC.17966.a.B1.GE38855

HARVESTED ON:	data unavailable
SEQUENCED ON:	1/15/2015
EXPECTED MW:	28kDa
OBSERVED MW:	99kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMS RGTRTFEIYR YDPDKDKAPY MQTFKLELTD KHRMLLDALL ALKVQDETLT FRRSCREGIC GSDGVNINGK NGLACLQNLN DLPEKIVIRP LPGLPVVKDL VVDMNQFYDQ YDKIQPFLIN NQPAPPKERL QSPEEREHLN GLYECILCAC CSTSCPSFWW NPDKFLGPSA LLNAYRFIID SRDTATAERL ARLDDPFSLF RCKGIMNCVS VCPKGLNPTK AIGHIRXXLL DQAG

Validated NT Sequence

atcttactca cttaacccgc ttgatctaac aacannntac ggatatgacc gattgctttt gttggattca aacctttagg gcatactgac acacaattca taataccttt acaacggaac aaactgaacg gatcgtcaag acgagccaaa cgttctgcag ttgcagtatc acgagagtcg ataataaaac gatatgcatt caacaacgct gaaggaccca agaatttatc agggttccac cagaatgatg ggcatgaagt tgaacaacat gcacaaagaa tacactcata caaaccattt aagtgttcac gctcttcagg agattgcaaa cgctctttag gtggagcagg ttggttattg atcaagaatg gctgaatttt gtcatactga tcatagaatt ggttcatatc aacaaccaaa tctttaacaa ccggcaaacc aggtaatgga cgaattacaa ttttctctgg taaatcgtta aggttttgca agcaagccaa accattttta ccattgatat tcacaccatc agaaccacaa ataccttcac ggcaagaacg acggaatgtt aatgtttcgt cttgaacctt caaagcaagc aatgcatcaa gcaacatacg gtgcttatca gtcaattcaa gcttgaaagt ttgcatgtac ggcgctttat ccttatcagg atcatagcgg tagatttcga atgtacgagt acctctactc atatggtggt ggtggtggtg agccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagta gaggtactcg tacattcgaa atctaccgct atgatcctga taaggataaa gcgccgtaca tgcaaacttt caagcttgaa ttgactgata agcaccgtat gttgcttgat gcattgcttg ctttgaaggt tcaagacgaa acattaacat tccgtcgttc ttgccgtgaa ggtatttgtg gttctgatgg tgtgaatatc aatggtaaaa atggtttggc ttgcttgcaa aaccttaacg atttaccaga gaaaattgta attcgtccat tacctggttt gccggttgtt aaagatttgg ttgttgatat gaaccaattc tatgatcagt atgacaaaat tcagccattc ttgatcaata accaacctgc tccacctaaa gagcgtttgc aatctcctga agagcgtgaa cacttaaatg gtttgtatga gtgtattctt tgtgcatgtt gttcaacttc atgcccatca ttctggtgga accctgataa attcttgggt ccttcagcgt tgttgaatgc atatcgtttt attatcgact ctcgtgatac tgcaactgca gaacgtttgg ctcgtcttga cgatccgttc agtttgttcc gttgtaaagg tattatgaat tgtgtgtcag tatgccctaa aggtttgaat ccaacaaaag caatcggtca tatccgtaat atgttgttag atcaagcggg ttgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat