AcbaC.17963.a

undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase

CENTER ID: AcbaC.17963.a
ORGANISM: Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS: cloned
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AcbaC.17963.a.B1.GE38853 full length 1 365
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|470.1288.peg.1434
GenBank: AKA29941.1
PGAT: acinetobacter:ABUW_0146
RefSeq: WP_000132435.1
UniProt: V5V8B1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
VTDSQQSKPK HVMMMAAGTG GHVFPALAVA KQLQQQGCQV SWLATPTGME NRLLKDQNIP IYQIDIQGVR GNGVIRKLAA PFKILKATFS AMRYMKQLKV DAVAGFGGYV AGPGGLAARL LGIPVLIHEQ NAVAGFTNAQ LSRVAKVVCE AFPNTFPASE KVVTTGNPVR REITDILSPK WRYDEREQAG KPLNILIVGG SLGAKALNER LPPALKQLEV PLNIFHQCGQ QQVEATQALY ADAPANLTVQ VLPFIEDMAK AYSEADLIIC RAGALTVTEV ATAGVAAVFV PLPIAVDDHQ TANAKFLADV GAAKICQQST MTPEVLNQLF TTLMNRQLLT EMAVKARQHA QPNATQHVVD LIQKM
NT Sequence
GTGACCGATT CACAGCAAAG CAAACCTAAA CATGTCATGA TGATGGCTGC CGGTACTGGT GGACATGTTT TTCCAGCCCT CGCCGTAGCC AAACAACTTC AACAACAAGG TTGTCAAGTT TCATGGTTAG CCACGCCAAC AGGTATGGAA AATCGTTTAT TAAAAGATCA AAATATTCCA ATTTATCAAA TTGATATTCA AGGTGTGCGT GGAAACGGAG TGATCCGTAA ACTTGCTGCA CCTTTTAAAA TTTTAAAAGC AACTTTTAGT GCAATGCGCT ATATGAAACA GCTTAAAGTG GATGCTGTTG CAGGCTTTGG GGGATATGTG GCAGGCCCGG GTGGGCTAGC AGCGCGTTTA TTAGGTATTC CTGTTCTCAT CCACGAGCAA AATGCGGTTG CAGGATTTAC CAATGCACAG TTATCCCGTG TTGCTAAAGT GGTATGTGAA GCGTTTCCAA ATACATTTCC TGCTAGCGAA AAAGTAGTGA CAACCGGCAA TCCGGTACGT CGTGAAATCA CTGATATCTT AAGTCCAAAG TGGCGCTATG ATGAACGCGA GCAAGCGGGT AAGCCGTTAA ATATTTTAAT TGTGGGCGGA TCTTTAGGTG CAAAAGCACT GAATGAACGC TTACCTCCGG CATTAAAACA ACTTGAAGTT CCACTGAATA TTTTCCATCA ATGTGGTCAG CAACAGGTTG AAGCAACTCA AGCTTTGTAC GCAGATGCGC CAGCAAATTT GACCGTACAG GTTTTGCCGT TTATTGAAGA TATGGCGAAA GCTTATAGCG AAGCAGACCT GATTATTTGC CGTGCTGGTG CTTTAACTGT GACAGAAGTA GCAACAGCTG GTGTTGCAGC AGTTTTTGTA CCGCTTCCTA TTGCGGTGGA TGACCACCAA ACTGCAAATG CCAAATTTCT GGCCGATGTC GGTGCTGCAA AAATTTGCCA GCAATCGACT ATGACACCAG AAGTTTTAAA TCAATTGTTT ACTACGCTGA TGAATCGCCA ATTACTTACA GAAATGGCAG TGAAAGCGCG TCAACATGCC CAACCAAATG CAACTCAGCA TGTGGTTGAT CTTATCCAAA AAATGTAA
Details for AcbaC.17963.a.B1.GE38853
HARVESTED ON: data unavailable
SEQUENCED ON: 1/15/2015
EXPECTED MW: 40kDa
OBSERVED MW: 20kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMV TDSQQSKPKH VMMMAAGTGG HVFPALAVAK QLQQQGCQVS WLATPTGMEN RLLKDQNIPI YQIDIQGVRG NGVIRKLAAP FKILKATFSA MRYMKQLKVD AVAGFGGYVA GPGGLAARLL GIPVLIHEQN AVAGFTNAQL SRVAKVVCEA FPNTFPASEK VVTTGNPVRR EITDILSPKW RYDEREQAGK PLNILIVGGS LGAKALNERL PPALKQLEVP LNIFHQCGQQ QVEATQALYA DAPANLTVQV LPFIEDMAKA YSEADLIICR AGALTVTEVA TAGVAAVFVP LPIAVDDHQT ANAKFLADVG AAKICQQSTM TPEVLNQLFT TLMNRQLLTE MAVKARQHAQ PNATQ
Validated NT Sequence
gctgagttgc atttggttgg gcatgttgac gcgctttcac tgccatttct gtaagtaatt ggcgattcat cagcgtagta aacaattgat ttaaaacttc tggtgtcata gtcgattgct gacaaatttt tgcagcaccg acatcggcca gaaatttggc atttgcagtt tggtggtcat ccaccgcaat aggaagcggt acaaaaactg ctgcaacacc agctgttgct acttctgtca cagttaaagc accagcacgg caaataatca ggtctgcttc gctataagct ttcgccatat cttcaataaa cggcaaaacc tgtacggtca aatttgctgg cgcatctgcg tacaaagctt gagttgcttc aacctgttgc tgaccacatt gatggaaaat attcagtgga acttcaagtt gttttaatgc cggaggtaag cgttcattca gtgcttttgc acctaaagat ccgcccacaa ttaaaatatt taacggctta cccgcttgct cgcgttcatc atagcgccac tttggactta agatatcagt gatttcacga cgtaccggat tgccggttgt cactactttt tcgctagcag gaaatgtatt tggaaacgct tcacatacca ctttagcaac acgggataac tgtgcattgg taaatcctgc aaccgcattt tgctcgtgga tgagaacagg aatacctaat aaacgcgctg ctagcccacc cgggcctgcc acatatcccc caaagcctgc aacagcatcc actttaagct gtttcatata gcgcattgca ctaaaagttg cttttaaaat tttaaaaggt gcagcaagtt tacggatcac tccgtttcca cgcacacctt gaatatcaat ttgataaatt ggaatatttt gatcttttaa taaacgattt tccatacctg ttggcgtggc taaccatgaa acttgacaac cttgttgttg aagttgtttg gctacggcga gggctggaaa aacatgtcca ccagtaccgg cagccatcat catgacatgt ttaggtttgc tttgctgtga atcggtcacc atatggtggt ggtggtggtg agccat
Expected Protein Sequence
MAHHHHHHVT DSQQSKPKHV MMMAAGTGGH VFPALAVAKQ LQQQGCQVSW LATPTGMENR LLKDQNIPIY QIDIQGVRGN GVIRKLAAPF KILKATFSAM RYMKQLKVDA VAGFGGYVAG PGGLAARLLG IPVLIHEQNA VAGFTNAQLS RVAKVVCEAF PNTFPASEKV VTTGNPVRRE ITDILSPKWR YDEREQAGKP LNILIVGGSL GAKALNERLP PALKQLEVPL NIFHQCGQQQ VEATQALYAD APANLTVQVL PFIEDMAKAY SEADLIICRA GALTVTEVAT AGVAAVFVPL PIAVDDHQTA NAKFLADVGA AKICQQSTMT PEVLNQLFTT LMNRQLLTEM AVKARQHAQP NATQHVVDLI QKM
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgaccg attcacagca aagcaaacct aaacatgtca tgatgatggc tgccggtact ggtggacatg tttttccagc cctcgccgta gccaaacaac ttcaacaaca aggttgtcaa gtttcatggt tagccacgcc aacaggtatg gaaaatcgtt tattaaaaga tcaaaatatt ccaatttatc aaattgatat tcaaggtgtg cgtggaaacg gagtgatccg taaacttgct gcacctttta aaattttaaa agcaactttt agtgcaatgc gctatatgaa acagcttaaa gtggatgctg ttgcaggctt tgggggatat gtggcaggcc cgggtgggct agcagcgcgt ttattaggta ttcctgttct catccacgag caaaatgcgg ttgcaggatt taccaatgca cagttatccc gtgttgctaa agtggtatgt gaagcgtttc caaatacatt tcctgctagc gaaaaagtag tgacaaccgg caatccggta cgtcgtgaaa tcactgatat cttaagtcca aagtggcgct atgatgaacg cgagcaagcg ggtaagccgt taaatatttt aattgtgggc ggatctttag gtgcaaaagc actgaatgaa cgcttacctc cggcattaaa acaacttgaa gttccactga atattttcca tcaatgtggt cagcaacagg ttgaagcaac tcaagctttg tacgcagatg cgccagcaaa tttgaccgta caggttttgc cgtttattga agatatggcg aaagcttata gcgaagcaga cctgattatt tgccgtgctg gtgctttaac tgtgacagaa gtagcaacag ctggtgttgc agcagttttt gtaccgcttc ctattgcggt ggatgaccac caaactgcaa atgccaaatt tctggccgat gtcggtgctg caaaaatttg ccagcaatcg actatgacac cagaagtttt aaatcaattg tttactacgc tgatgaatcg ccaattactt acagaaatgg cagtgaaagc gcgtcaacat gcccaaccaa atgcaactca gcatgtggtt gatcttatcc aaaaaatgtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat