AcbaC.17955.a

DNA polymerase III, delta subunit

CENTER ID: AcbaC.17955.a
ORGANISM: Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AcbaC.17955.a.B1.GE38849 full length 1 329
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|470.1345.peg.3296
GenBank: AKA33057.1
PGAT: acinetobacter:ABUW_3361
RefSeq: WP_000762831.1
UniProt: V5VIA8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKLDYLQALK RIPEARGAWI LHGQEPLLEQ NLLDTFRKSW QQQDIERQRY DISSVSDWKN VFNALNSLSL FSQQLAIEVH GNIKPDANGL KQLKSYIQHN ETNLLLIVLP KQDSSSLKSA FFQVVEANGV VVALTANYPQ DRQRILAAEA EKLEIQLDND AWQWLMQHHE HNLLAAKNSL MRVRDTFPDQ KLIQIEQLYA CLQDQSRYTT YDLSDALLEG NLAQSIKIFQ YLIGSGEPES LILWTLSKEM RLLMQLFEQP HNALQLGIWK TKVSLYQQAL RRLNPQQFLG WSALLLQIDA AIKGMSNENA QHLMQQAIAE LCGKTLFIH
NT Sequence
ATGAAACTTG ACTATTTACA AGCCCTCAAA CGCATTCCGG AAGCTCGGGG TGCGTGGATT CTGCATGGAC AGGAACCTTT ACTAGAACAA AATTTACTCG ATACTTTCCG CAAAAGCTGG CAGCAACAAG ACATTGAAAG ACAGCGCTAC GATATTAGTA GTGTAAGTGA CTGGAAAAAT GTCTTTAACG CACTGAACAG CCTATCTTTA TTTTCTCAGC AACTTGCGAT TGAAGTTCAC GGCAATATCA AGCCCGATGC CAATGGTTTA AAACAGCTAA AAAGTTATAT CCAGCATAAT GAAACTAACC TGCTGTTAAT TGTTTTACCC AAACAAGACA GTAGCAGCTT AAAATCTGCT TTTTTCCAAG TTGTTGAAGC GAACGGTGTG GTGGTTGCCC TTACTGCTAA CTATCCGCAA GATCGTCAAC GAATATTAGC CGCTGAGGCT GAGAAGTTAG AAATTCAACT CGACAATGAT GCTTGGCAGT GGCTCATGCA GCATCATGAG CATAACCTGC TCGCTGCTAA AAATAGCTTG ATGCGGGTAA GAGATACTTT TCCAGACCAG AAACTAATTC AAATTGAACA GTTATATGCG TGTCTACAAG ATCAGTCTCG TTACACCACT TACGATTTAA GCGATGCGTT ATTAGAAGGT AATCTTGCTC AGTCCATTAA GATCTTTCAA TATTTAATCG GTTCCGGTGA ACCGGAAAGC TTGATCTTAT GGACATTAAG TAAAGAAATG CGTTTATTAA TGCAGTTGTT TGAACAACCA CATAATGCAT TACAACTAGG TATATGGAAA ACAAAAGTTA GCTTATATCA ACAAGCATTA AGAAGACTCA ATCCACAGCA ATTTTTGGGA TGGTCTGCCC TGCTTTTACA AATTGATGCC GCAATCAAAG GCATGTCAAA TGAGAATGCC CAACATTTAA TGCAACAAGC CATTGCCGAG CTGTGTGGAA AAACATTATT TATACATTAG
Details for AcbaC.17955.a.B1.GE38849
HARVESTED ON: 1/29/2015
SEQUENCED ON: 1/15/2015
EXPECTED MW: 39kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMK LDYLQALKRI PEARGAWILH GQEPLLEQNL LDTFRKSWQQ QDIERQRYDI SSVSDWKNVF NALNSLSLFS QQLAIEVHGN IKPDANGLKQ LKSYIQHNET NLLLIVLPKQ DSSSLKSAFF QVVEANGVVV ALTANYPQDR QRILAAEAEK LEIQLDNDAW QWLMQHHEHN LLAAKNSLMR VRDTFPDQKL IQIEQLYACL QDQSRYTTYD LSDALLEGNL AQSIKIFQYL IXXGEPXXLI LWTLSKEMRL LMQLFEQPHN ALQLGIWKTK VSLYQQALRR LNPQQFLGWS ALLLQIDAAI KGMSNENAQH LMQQAIAEXX GKTLFIH
Validated NT Sequence
ttaatgtata aataatgttt ttccnnnnnn ctcggcaatg gcttgttgca ttaaatgttg ggcattctca tttgacatgc ctttgattgc ggcatcaatt tgtaaaagca gggcagacca tcccaaaaat tgctgtggat tgagtcttct taatgcttgt tgatataagc taacttttgt tttccatata cctagttgta atgcattatg tggttgttca aacaactgca ttaataaacg catttcttta cttaatgtcc ataagatcaa gnnnnnnggt tcaccggnnn cgattaaata ttgaaagatc ttaatggact gagcaagatt accttctaat aacgcatcgc ttaaatcgta agtggtgtaa cgagactgat cttgtagaca cgcatataac tgttcaattt gaattagttt ctggtctgga aaagtatctc ttacccgcat caagctattt ttagcagcga gcaggttatg ctcatgatgc tgcatgagcc actgccaagc atcattgtcg agttgaattt ctaacttctc agcctcagcg gctaatattc gttgacgatc ttgcggatag ttagcagtaa gggcaaccac cacaccgttc gcttcaacaa cttggaaaaa agcagatttt aagctgctac tgtcttgttt gggtaaaaca attaacagca ggttagtttc attatgctgg atataacttt ttagctgttt taaaccattg gcatcgggct tgatattgcc gtgaacttca atcgcaagtt gctgagaaaa taaagatagg ctgttcagtg cgttaaagac atttttccag tcacttacac tactaatatc gtagcgctgt ctttcaatgt cttgttgctg ccagcttttg cggaaagtat cgagtaaatt ttgttctagt aaaggttcct gtccatgcag aatccacgca ccccgagctt ccggaatgcg tttgagggct tgtaaatagt caagtttcat atggtggtgg tggtggtgag ccat
Expected Protein Sequence
MAHHHHHHMK LDYLQALKRI PEARGAWILH GQEPLLEQNL LDTFRKSWQQ QDIERQRYDI SSVSDWKNVF NALNSLSLFS QQLAIEVHGN IKPDANGLKQ LKSYIQHNET NLLLIVLPKQ DSSSLKSAFF QVVEANGVVV ALTANYPQDR QRILAAEAEK LEIQLDNDAW QWLMQHHEHN LLAAKNSLMR VRDTFPDQKL IQIEQLYACL QDQSRYTTYD LSDALLEGNL AQSIKIFQYL IGSGEPESLI LWTLSKEMRL LMQLFEQPHN ALQLGIWKTK VSLYQQALRR LNPQQFLGWS ALLLQIDAAI KGMSNENAQH LMQQAIAELC GKTLFIH
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaac ttgactattt acaagccctc aaacgcattc cggaagctcg gggtgcgtgg attctgcatg gacaggaacc tttactagaa caaaatttac tcgatacttt ccgcaaaagc tggcagcaac aagacattga aagacagcgc tacgatatta gtagtgtaag tgactggaaa aatgtcttta acgcactgaa cagcctatct ttattttctc agcaacttgc gattgaagtt cacggcaata tcaagcccga tgccaatggt ttaaaacagc taaaaagtta tatccagcat aatgaaacta acctgctgtt aattgtttta cccaaacaag acagtagcag cttaaaatct gcttttttcc aagttgttga agcgaacggt gtggtggttg cccttactgc taactatccg caagatcgtc aacgaatatt agccgctgag gctgagaagt tagaaattca actcgacaat gatgcttggc agtggctcat gcagcatcat gagcataacc tgctcgctgc taaaaatagc ttgatgcggg taagagatac ttttccagac cagaaactaa ttcaaattga acagttatat gcgtgtctac aagatcagtc tcgttacacc acttacgatt taagcgatgc gttattagaa ggtaatcttg ctcagtccat taagatcttt caatatttaa tcggttccgg tgaaccggaa agcttgatct tatggacatt aagtaaagaa atgcgtttat taatgcagtt gtttgaacaa ccacataatg cattacaact aggtatatgg aaaacaaaag ttagcttata tcaacaagca ttaagaagac tcaatccaca gcaatttttg ggatggtctg ccctgctttt acaaattgat gccgcaatca aaggcatgtc aaatgagaat gcccaacatt taatgcaaca agccattgcc gagctgtgtg gaaaaacatt atttatacat tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat