SSGCID: Target Page

AcbaC.17951.a

aspartate kinase

CENTER ID:	AcbaC.17951.a
ORGANISM:	Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AcbaC.17951.a.B1.GE38847	full length		1	426

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|470.1345.peg.2702
GenBank:	AKA32466.1
PGAT:	acinetobacter:ABUW_2749
RefSeq:	WP_001185176.1
UniProt:	V5VES9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MALIVQKYGG TSMGTPERIL NVARRVKRWH DHGHKVVVVV SAMSGETNRL LALAKAITET PDPRELDQMV STGEQVTISM LAMALNSIGV EAKSYTGRQV GIKTDSAFTK ARIESIDTDV MTNDLDAGRV IVVAGFQGFD ANGNTTTLGR GGSDTSGVAI AAALKADECQ IYTDVDGVYT TDPRVAPKAK KIDRISFEEM LEMASLGSKV LQIRSVEFAG KYQVPLRVLS SFDNDDDGAF DDEFKENVGT LITTEAEDTM EQPIIAGIAF NRDEAKLTIL GVPDEPGIAS KILSPVSDAN IEVDMIVQNV EEDGTTDFTF TVNRVDLAKA EKILNETAKN IGAREVSTRD DIVKVSIVGV GMRSHAGVAS KMFTALADEG INILMISTSE IKVSVIIDEK YLELAVRCLH TAFGLDREHG ESSARA

NT Sequence

ATGGCATTAA TCGTTCAAAA ATATGGCGGT ACTTCTATGG GTACCCCCGA GCGCATTTTA AATGTTGCTC GTCGTGTGAA GCGTTGGCAT GATCACGGTC ACAAGGTTGT TGTGGTCGTA TCTGCAATGA GTGGTGAAAC AAACCGCTTA CTTGCCCTCG CTAAAGCCAT TACCGAAACA CCTGACCCAC GCGAGTTAGA CCAAATGGTT TCAACCGGTG AACAGGTGAC GATTTCCATG TTAGCTATGG CACTTAATTC GATTGGTGTG GAAGCTAAAT CCTATACCGG ACGTCAAGTC GGTATTAAAA CTGACAGTGC ATTTACTAAG GCGCGTATTG AGTCGATTGA CACAGATGTC ATGACCAATG ACCTAGATGC AGGCCGTGTT ATTGTAGTTG CAGGCTTCCA AGGCTTCGAT GCCAACGGAA ATACTACAAC TTTAGGACGT GGCGGTTCAG ATACTTCTGG TGTTGCCATT GCAGCTGCCC TAAAAGCAGA TGAATGCCAA ATTTATACTG ACGTTGATGG GGTTTATACA ACCGACCCTC GTGTTGCCCC TAAAGCGAAG AAAATCGATC GTATTTCTTT TGAAGAAATG TTAGAAATGG CATCATTAGG TTCTAAAGTT TTACAGATTC GCTCAGTAGA ATTTGCCGGA AAATATCAAG TTCCTTTACG CGTATTATCA AGTTTTGATA ATGATGACGA CGGCGCATTT GACGACGAAT TTAAAGAAAA TGTAGGTACA CTCATTACTA CTGAGGCAGA AGACACTATG GAACAGCCAA TCATTGCAGG TATCGCTTTT AACCGTGACG AAGCAAAATT AACAATTTTA GGTGTACCTG ATGAACCGGG TATTGCTTCT AAAATTTTAT CACCAGTAAG TGATGCCAAC ATTGAAGTGG ATATGATAGT CCAAAATGTT GAAGAAGATG GCACAACAGA TTTCACGTTT ACTGTAAACC GTGTTGATTT AGCAAAAGCT GAAAAAATCT TAAATGAAAC TGCCAAAAAT ATTGGTGCAC GTGAAGTTTC AACACGCGAT GACATCGTTA AAGTATCTAT CGTGGGTGTA GGTATGCGCT CGCATGCGGG TGTAGCAAGC AAAATGTTTA CTGCCCTTGC TGACGAAGGT ATTAACATCC TTATGATTTC AACATCTGAA ATTAAGGTTT CTGTCATTAT TGATGAAAAA TACCTTGAGC TTGCAGTTCG TTGCTTACAT ACCGCTTTTG GTTTAGATCG TGAACATGGT GAAAGCAGCG CACGCGCTTA A

Details for AcbaC.17951.a.B1.GE38847

HARVESTED ON:	1/29/2015
SEQUENCED ON:	1/15/2015
EXPECTED MW:	47kDa
OBSERVED MW:	47kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMA LIVQKYGGTS MGTPERILNV ARRVKRWHDH GHKVVVVVSA MSGETNRLLA LAKAITETPD PRELDQMVST GEQVTISMLA MALNSIGVEA KSYTGRQVGI KTDSAFTKAR IESIDTDVMT NDLDAGRVIV VAGFQGFDAN GNTTTLGRGG SDTSGVAIAA ALKADECQIY TDVDGVYTTD PRVAPKAKKI DRISFEEMLE MASLGSKVLQ IRSVEFAGKY QVPLRVLSSF DNDDDGAFDD EFKENVGTLI TTEAEDTMEQ PIIAGIAFNR DEAKLTILGV PDEPGIASKI LSPVSDANIE VDMIVQNVEE DGTTDFTFTV NRVDLAKAEK ILNETAKNIG AREVSTRDDI VKVSIVGVGM RSHAGVASKM FTALADEGIN ILMISTSEIK VSVIIDEKYL ELAVRCLHTA FGLDREH

Validated NT Sequence

catgttcacg atctaaacca aaagcggtat gtaagcaacg aactgcaagc tcaaggtatt tttcatcaat aatgacagaa accttaattt cagatgttga aatcataagg atgttaatac cttcgtcagc aagggcagta aacattttgc ttgctacacc cgcatgcgag cgcataccta cacccacgat agatacttta acgatgtcat cgcgtgttga aacttcacgt gcaccaatat ttttggcagt ttcatttaag attttttcag cttttgctaa atcaacacgg tttacagtaa acgtgaaatc tgttgtgcca tcttcttcaa cattttggac tatcatatcc acttcaatgt tggcatcact tactggtgat aaaattttag aagcaatacc cggttcatca ggtacaccta aaattgttaa ttttgcttcg tcacggttaa aagcgatacc tgcaatgatt ggctgttcca tagtgtcttc tgcctcagta gtaatgagtg tacctacatt ttctttaaat tcgtcgtcaa atgcgccgtc gtcatcatta tcaaaacttg ataatacgcg taaaggaact tgatattttc cggcaaattc tactgagcga atctgtaaaa ctttagaacc taatgatgcc atttctaaca tttcttcaaa agaaatacga tcgattttct tcgctttagg ggcaacacga gggtcggttg tataaacccc atcaacgtca gtataaattt ggcattcatc tgcttttagg gcagctgcaa tggcaacacc agaagtatct gaaccgccac gtcctaaagt tgtagtattt ccgttggcat cgaagccttg gaagcctgca actacaataa cacggcctgc atctaggtca ttggtcatga catctgtgtc aatcgactca atacgcgcct tagtaaatgc actgtcagtt ttaataccga cttgacgtcc ggtataggat ttagcttcca caccaatcga attaagtgcc atagctaaca tggaaatcgt cacctgttca ccggttgaaa ccatttggtc taactcgcgt gggtcaggtg tttcggtaat ggctttagcg agggcaagta agcggtttgt ttcaccactc attgcagata cgaccacaac aaccttgtga ccgtgatcat gccaacgctt cacacgacga gcaacattta aaatgcgctc gggggtaccc atagaagtac cgccatattt ttgaacgatt aatgccatat ggtggtggtg gtggtgagcc at

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcat taatcgttca aaaatatggc ggtacttcta tgggtacccc cgagcgcatt ttaaatgttg ctcgtcgtgt gaagcgttgg catgatcacg gtcacaaggt tgttgtggtc gtatctgcaa tgagtggtga aacaaaccgc ttacttgccc tcgctaaagc cattaccgaa acacctgacc cacgcgagtt agaccaaatg gtttcaaccg gtgaacaggt gacgatttcc atgttagcta tggcacttaa ttcgattggt gtggaagcta aatcctatac cggacgtcaa gtcggtatta aaactgacag tgcatttact aaggcgcgta ttgagtcgat tgacacagat gtcatgacca atgacctaga tgcaggccgt gttattgtag ttgcaggctt ccaaggcttc gatgccaacg gaaatactac aactttagga cgtggcggtt cagatacttc tggtgttgcc attgcagctg ccctaaaagc agatgaatgc caaatttata ctgacgttga tggggtttat acaaccgacc ctcgtgttgc ccctaaagcg aagaaaatcg atcgtatttc ttttgaagaa atgttagaaa tggcatcatt aggttctaaa gttttacaga ttcgctcagt agaatttgcc ggaaaatatc aagttccttt acgcgtatta tcaagttttg ataatgatga cgacggcgca tttgacgacg aatttaaaga aaatgtaggt acactcatta ctactgaggc agaagacact atggaacagc caatcattgc aggtatcgct tttaaccgtg acgaagcaaa attaacaatt ttaggtgtac ctgatgaacc gggtattgct tctaaaattt tatcaccagt aagtgatgcc aacattgaag tggatatgat agtccaaaat gttgaagaag atggcacaac agatttcacg tttactgtaa accgtgttga tttagcaaaa gctgaaaaaa tcttaaatga aactgccaaa aatattggtg cacgtgaagt ttcaacacgc gatgacatcg ttaaagtatc tatcgtgggt gtaggtatgc gctcgcatgc gggtgtagca agcaaaatgt ttactgccct tgctgacgaa ggtattaaca tccttatgat ttcaacatct gaaattaagg tttctgtcat tattgatgaa aaataccttg agcttgcagt tcgttgctta cataccgctt ttggtttaga tcgtgaacat ggtgaaagca gcgcacgcgc ttgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat