AcbaC.17943.a

leucyl-tRNA synthetase

CENTER ID: AcbaC.17943.a
ORGANISM: Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AcbaC.17943.a.B1.GE38843 full length 1 874
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AcbaC.17943.a.B1.PW37693 full length 1 874

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|470.1345.peg.3294
GenBank: AKA33055.1
PGAT: acinetobacter:ABUW_3359
RefSeq: WP_000155773.1
UniProt: V5VGT4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTISHIDPEY QANTIEPSVQ QDWENRKVFK VADTVEGKHR YILSMFPYPS GKLHMGHVRN YTIGDVISRF YRLKGETVLQ PMGWDAFGLP AENAAIAHKV APAKWTFENI AYMRDQLKKL GLSVDWDREF ATCTPEYYHW EQWLFVQLYK KGLIYRKLST VNWDPVDQTV LANEQVENGR GWRSGALVEK RDIPMYYFRI TDYAQELLDD LDTLQDGWPQ QVLTMQRNWI GRSTGMEITF PSANTEIYAD GLTVYTTRAD TLMGVTYVAV AAEHPLALKA AENNPELAAF IEECRMGSVA EADLATAEKK GMATGLFVKH PVTGEELPVW IANYVLMSYG SGAVMAVPAH DERDFEFANK FNLPIKQVID AKGADDADYS ATEWQEWYGS KEGKLVNSGE FDGLEFQAAF DAFLAKLEPQ GLANSKVQFR LRDWGVSRQR YWGCPIPMIN CDTCGQVTVP EDQLPVVLPT DVVPDGSGNP LNKMPEFYET KCPCCGGDAR RETDTLDTFV ESSWYYARYA SPDFTGGMVK PEAAKNWLPV NQYIGGVEHA ILHLLYARFF HKLMRDEGVV QGNEPFTNLL TQGMVLADTF YREAENGKKT WFNPADIELE RDEKGRIISA KYSGDGQEVI IGGQEKMSKS KNNGIDPQAI IDQYGADTAR VFMMFAAPPD QSLEWSDAGV EGANRFLKRV WRLVASFLEK GNSATAIDKA NLSKDAQDLR RKTHETIQKV SDDIERRHAF NTAIAALMEL LNASNKFEAK DDNDVAVERE AITTLLTLLA PFAPHLSQTL LAQFGTDLTE ATFPEVDASA LTRNTQTIVV QVNGKLRGKL EVSVDISKDE LLAQAKALPE VQQFLTGPTK KEIVVPNKLV NLVV
NT Sequence
ATGACTATTT CTCACATTGA CCCAGAATAT CAAGCAAACA CGATTGAACC ATCAGTCCAA CAAGATTGGG AAAATCGTAA AGTCTTTAAA GTTGCCGACA CTGTCGAAGG TAAACATCGC TACATCCTGT CAATGTTCCC TTACCCAAGT GGCAAACTGC ACATGGGTCA TGTGCGTAAC TATACCATTG GCGATGTAAT TAGCCGTTTT TACCGTTTAA AAGGCGAAAC CGTATTACAA CCTATGGGTT GGGATGCATT TGGTTTACCA GCGGAAAACG CTGCAATTGC CCATAAAGTT GCTCCGGCAA AATGGACATT TGAAAACATC GCTTACATGC GTGACCAATT AAAAAAATTA GGTCTATCTG TAGATTGGGA CCGTGAATTT GCAACGTGTA CACCAGAGTA CTATCACTGG GAACAATGGT TGTTCGTTCA GCTTTATAAA AAAGGTTTAA TCTACCGTAA ACTTTCAACA GTAAACTGGG ATCCGGTTGA CCAAACAGTT TTGGCAAACG AACAAGTTGA AAATGGTCGT GGCTGGCGTT CAGGTGCATT GGTTGAAAAA CGCGATATTC CAATGTACTA CTTCCGTATT ACGGACTATG CACAAGAATT ATTAGACGAC CTAGACACTC TACAAGACGG CTGGCCTCAA CAAGTGTTGA CCATGCAGCG TAACTGGATT GGTCGCTCTA CTGGTATGGA AATTACCTTC CCATCTGCCA ATACTGAAAT TTATGCTGAC GGCTTAACTG TTTATACAAC ACGTGCCGAC ACACTTATGG GGGTGACGTA CGTTGCCGTT GCAGCAGAAC ACCCTCTTGC GCTTAAAGCT GCTGAAAATA ACCCTGAATT GGCTGCATTT ATTGAAGAAT GCCGCATGGG TTCAGTTGCA GAAGCTGACT TGGCTACAGC CGAGAAGAAA GGTATGGCAA CAGGTTTGTT TGTAAAACAC CCTGTAACTG GCGAAGAGCT TCCTGTCTGG ATTGCGAACT ACGTATTAAT GTCTTACGGT TCTGGCGCAG TGATGGCTGT ACCAGCACAT GACGAACGTG ACTTTGAATT TGCTAACAAG TTCAACTTGC CAATTAAGCA AGTGATTGAT GCCAAAGGTG CTGACGATGC AGATTATTCT GCTACCGAGT GGCAAGAATG GTACGGTTCT AAAGAAGGTA AACTTGTTAA CTCTGGCGAG TTTGACGGTC TAGAATTCCA AGCTGCATTC GATGCATTCC TTGCGAAATT AGAACCACAA GGTTTAGCAA ATTCTAAAGT TCAATTCCGT TTACGTGACT GGGGTGTTTC TCGTCAACGT TACTGGGGTT GTCCAATTCC AATGATTAAC TGTGATACTT GTGGTCAAGT CACAGTTCCT GAAGATCAAC TTCCGGTTGT ATTACCAACT GACGTTGTTC CAGATGGTTC AGGTAATCCA CTAAATAAAA TGCCTGAATT CTATGAAACG AAATGTCCTT GCTGTGGTGG CGATGCGCGC CGTGAAACAG ATACATTGGA CACATTCGTA GAATCATCTT GGTACTACGC ACGCTATGCA TCTCCAGACT TTACTGGTGG TATGGTAAAA CCTGAAGCAG CTAAAAACTG GCTTCCTGTA AACCAATATA TTGGTGGTGT TGAACACGCG ATTCTTCACT TACTTTATGC ACGTTTCTTC CACAAATTAA TGCGTGATGA AGGTGTTGTA CAAGGTAATG AGCCATTTAC CAACTTGTTA ACTCAAGGCA TGGTGCTTGC AGATACTTTC TATCGTGAAG CAGAAAATGG TAAGAAAACT TGGTTCAACC CTGCTGACAT CGAATTAGAA CGTGATGAGA AAGGCCGCAT CATTTCTGCA AAATATTCAG GTGATGGTCA AGAAGTGATT ATCGGCGGAC AAGAAAAAAT GTCCAAGTCG AAAAATAACG GTATTGACCC ACAAGCTATT ATTGATCAAT ACGGCGCAGA TACAGCTCGT GTATTTATGA TGTTTGCTGC ACCACCAGAT CAATCTTTAG AATGGTCTGA TGCAGGTGTT GAAGGTGCAA ACCGTTTCTT GAAACGTGTA TGGCGTCTGG TAGCAAGCTT CCTTGAAAAA GGAAACTCTG CAACAGCAAT CGATAAAGCA AACTTGTCTA AAGATGCTCA AGATTTACGT CGTAAGACAC ACGAAACCAT TCAAAAAGTA AGTGATGATA TTGAGCGTCG TCATGCGTTC AATACTGCAA TTGCTGCATT GATGGAATTG TTAAATGCAA GCAATAAGTT TGAAGCAAAA GATGACAATG ACGTTGCTGT AGAACGTGAA GCAATCACCA CATTGTTAAC TTTACTTGCT CCATTTGCAC CACATTTAAG CCAGACTTTA TTGGCTCAGT TTGGTACGGA CTTAACAGAA GCAACTTTCC CTGAAGTTGA TGCTTCTGCA TTAACCCGCA ATACTCAAAC GATTGTTGTA CAAGTAAATG GTAAACTTCG CGGTAAACTT GAAGTTTCTG TTGATATTTC TAAAGATGAG TTATTAGCTC AAGCGAAAGC ATTACCAGAA GTTCAGCAGT TCTTAACAGG ACCAACCAAA AAAGAGATTG TGGTTCCAAA CAAATTGGTT AACCTCGTGG TTTAA
Details for AcbaC.17943.a.B1.GE38843
HARVESTED ON: 1/29/2015
SEQUENCED ON: 1/15/2015
EXPECTED MW: 99kDa
OBSERVED MW: 99kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMT ISHIDPEYQA NTIEPSVQQD WENRKVFKVA DTVEGKHRYI LSMFPYPSGK LHMGHVRNYT IGDVISRFYR LKGETVLQPM GWDAFGLPAE NAAIAHKVAP AKWTFENIAY MRDQLKKLGL SVDWDREFAT CTPEYYHWEQ WLFVQLYKKG LIYRKLSTVN WDPVDQTVLA NEQVENGRGW RSGALVEKRD IPMYYFRITD YAQELLDDLD TLQDGWPQQV LTMQRNWIGR STGMEITFPS ANTEIYADGL TVYTTRADTL MGVTYVAVAA EHPLALKAAE NNPELAAFIE ECRMGSVAEA DLATAEKKGM ATGLFVKHPV TGEELPVWIA NYVLMSYGSG AVMAVPAHDE RDFEFANKFN LPIKQVIDAK GADDADYSAT EWQEWYGSKE GKLVNSGEFD GLEFQAAFDA FLAKLEPQGL ANSKVQFRLR DWGVSRQRYW GCPIPMINCD TCGQVTVPED QLPVVLPTDV VPDGSGNPLN KMPEFYETKC PCCGGDARRE TDTLDTFVES SWYYARYASP DFTGGMVKPE AAKNWLPVNQ YIGGVEHAIL HLLYARFFHK LMRDEGVVQG NEPFTNLLTQ GMVLADTFYR EAENGKKTWF NPADIELERD EKGRIISAKY SGDGQEVIIG GQEKMSKSKN NGIDPQAIID QYGADTARVF MMFAAPPDQS LEWSDAGVEG ANRFLKRVWR LVASFLEKGN SATAIDKANL SKDAQDLRRK THETIQKVSD DIERRHAFNT AIAALMELLN ASNKFEAKDD NDVAVEREAI TTLLTLLAPF APHLSQTLLA QFGTDLTEAT FPEVDASALT RNTQTIVVQV NGKLRGKLEV SVDISKDELL AQAKALPEVQ QFLTGPTKKE IVVPNKLVNL VV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacta tttctcacat tgacccagaa tatcaagcaa acacgattga accatcagtc caacaagatt gggaaaatcg taaagtcttt aaagttgccg acactgtcga aggtaaacat cgctacatcc tgtcaatgtt cccttaccca agtggcaaac tgcacatggg tcatgtgcgt aactatacca ttggcgatgt aattagccgt ttttaccgtt taaaaggcga aaccgtatta caacctatgg gttgggatgc atttggttta ccagcggaaa acgctgcaat tgcccataaa gttgctccgg caaaatggac atttgaaaac atcgcttaca tgcgtgacca attaaaaaaa ttaggtctat ctgtagattg ggaccgtgaa tttgcaacgt gtacaccaga gtactatcac tgggaacaat ggttgttcgt tcagctttat aaaaaaggtt taatctaccg taaactttca acagtaaact gggatccggt tgaccaaaca gttttggcaa acgaacaagt tgaaaatggt cgtggctggc gttcaggtgc attggttgaa aaacgcgata ttccaatgta ctacttccgt attacggact atgcacaaga attattagac gacctagaca ctctacaaga cggctggcct caacaagtgt tgaccatgca gcgtaactgg attggtcgct ctactggtat ggaaattacc ttcccatctg ccaatactga aatttatgct gacggcttaa ctgtttatac aacacgtgcc gacacactta tgggggtgac gtacgttgcc gttgcagcag aacaccctct tgcgcttaaa gctgctgaaa ataaccctga attggctgca tttattgaag aatgccgcat gggttcagtt gcagaagctg acttggctac agccgagaag aaaggtatgg caacaggttt gtttgtaaaa caccctgtaa ctggcgaaga gcttcctgtc tggattgcga actacgtatt aatgtcttac ggttctggcg cagtgatggc tgtaccagca catgacgaac gtgactttga atttgctaac aagttcaact tgccaattaa gcaagtgatt gatgccaaag gtgctgacga tgcagattat tctgctaccg agtggcaaga atggtacggt tctaaagaag gtaaacttgt taactctggc gagtttgacg gtctagaatt ccaagctgca ttcgatgcat tccttgcgaa attagaacca caaggtttag caaattctaa agttcaattc cgtttacgtg actggggtgt ttctcgtcaa cgttactggg gttgtccaat tccaatgatt aactgtgata cttgtggtca agtcacagtt cctgaagatc aacttccggt tgtattacca actgacgttg ttccagatgg ttcaggtaat ccactaaata aaatgcctga attctatgaa acgaaatgtc cttgctgtgg tggcgatgcg cgccgtgaaa cagatacatt ggacacattc gtagaatcat cttggtacta cgcacgctat gcatctccag actttactgg tggtatggta aaacctgaag cagctaaaaa ctggcttcct gtaaaccaat atattggtgg tgttgaacac gcgattcttc acttacttta tgcacgtttc ttccacaaat taatgcgtga tgaaggtgtt gtacaaggta atgagccatt taccaacttg ttaactcaag gcatggtgct tgcagatact ttctatcgtg aagcagaaaa tggtaagaaa acttggttca accctgctga catcgaatta gaacgtgatg agaaaggccg catcatttct gcaaaatatt caggtgatgg tcaagaagtg attatcggcg gacaagaaaa aatgtccaag tcgaaaaata acggtattga cccacaagct attattgatc aatacggcgc agatacagct cgtgtattta tgatgtttgc tgcaccacca gatcaatctt tagaatggtc tgatgcaggt gttgaaggtg caaaccgttt cttgaaacgt gtatggcgtc tggtagcaag cttccttgaa aaaggaaact ctgcaacagc aatcgataaa gcaaacttgt ctaaagatgc tcaagattta cgtcgtaaga cacacgaaac cattcaaaaa gtaagtgatg atattgagcg tcgtcatgcg ttcaatactg caattgctgc attgatggaa ttgttaaatg caagcaataa gtttgaagca aaagatgaca atgacgttgc tgtagaacgt gaagcaatca ccacattgtt aactttactt gctccatttg caccacattt aagccagact ttattggctc agtttggtac ggacttaaca gaagcaactt tccctgaagt tgatgcttct gcattaaccc gcaatactca aacgattgtt gtacaagtaa atggtaaact tcgcggtaaa cttgaagttt ctgttgatat ttctaaagat gagttattag ctcaagcgaa agcattacca gaagttcagc agttcttaac aggaccaacc aaaaaagaga ttgtggttcc aaacaaattg gttaacctcg tggtttgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for AcbaC.17943.a.B1.PW37693
PURIFICATION DATe: 4/7/2015
CONCENTRATION: 27mg/ml
OBSERVED MW: 100kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 200µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMT ISHIDPEYQA NTIEPSVQQD WENRKVFKVA DTVEGKHRYI LSMFPYPSGK LHMGHVRNYT IGDVISRFYR LKGETVLQPM GWDAFGLPAE NAAIAHKVAP AKWTFENIAY MRDQLKKLGL SVDWDREFAT CTPEYYHWEQ WLFVQLYKKG LIYRKLSTVN WDPVDQTVLA NEQVENGRGW RSGALVEKRD IPMYYFRITD YAQELLDDLD TLQDGWPQQV LTMQRNWIGR STGMEITFPS ANTEIYADGL TVYTTRADTL MGVTYVAVAA EHPLALKAAE NNPELAAFIE ECRMGSVAEA DLATAEKKGM ATGLFVKHPV TGEELPVWIA NYVLMSYGSG AVMAVPAHDE RDFEFANKFN LPIKQVIDAK GADDADYSAT EWQEWYGSKE GKLVNSGEFD GLEFQAAFDA FLAKLEPQGL ANSKVQFRLR DWGVSRQRYW GCPIPMINCD TCGQVTVPED QLPVVLPTDV VPDGSGNPLN KMPEFYETKC PCCGGDARRE TDTLDTFVES SWYYARYASP DFTGGMVKPE AAKNWLPVNQ YIGGVEHAIL HLLYARFFHK LMRDEGVVQG NEPFTNLLTQ GMVLADTFYR EAENGKKTWF NPADIELERD EKGRIISAKY SGDGQEVIIG GQEKMSKSKN NGIDPQAIID QYGADTARVF MMFAAPPDQS LEWSDAGVEG ANRFLKRVWR LVASFLEKGN SATAIDKANL SKDAQDLRRK THETIQKVSD DIERRHAFNT AIAALMELLN ASNKFEAKDD NDVAVEREAI TTLLTLLAPF APHLSQTLLA QFGTDLTEAT FPEVDASALT RNTQTIVVQV NGKLRGKLEV SVDISKDELL AQAKALPEVQ QFLTGPTKKE IVVPNKLVNL VV
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACTA TTTCTCACAT TGACCCAGAA TATCAAGCAA ACACGATTGA ACCATCAGTC CAACAAGATT GGGAAAATCG TAAAGTCTTT AAAGTTGCCG ACACTGTCGA AGGTAAACAT CGCTACATCC TGTCAATGTT CCCTTACCCA AGTGGCAAAC TGCACATGGG TCATGTGCGT AACTATACCA TTGGCGATGT AATTAGCCGT TTTTACCGTT TAAAAGGCGA AACCGTATTA CAACCTATGG GTTGGGATGC ATTTGGTTTA CCAGCGGAAA ACGCTGCAAT TGCCCATAAA GTTGCTCCGG CAAAATGGAC ATTTGAAAAC ATCGCTTACA TGCGTGACCA ATTAAAAAAA TTAGGTCTAT CTGTAGATTG GGACCGTGAA TTTGCAACGT GTACACCAGA GTACTATCAC TGGGAACAAT GGTTGTTCGT TCAGCTTTAT AAAAAAGGTT TAATCTACCG TAAACTTTCA ACAGTAAACT GGGATCCGGT TGACCAAACA GTTTTGGCAA ACGAACAAGT TGAAAATGGT CGTGGCTGGC GTTCAGGTGC ATTGGTTGAA AAACGCGATA TTCCAATGTA CTACTTCCGT ATTACGGACT ATGCACAAGA ATTATTAGAC GACCTAGACA CTCTACAAGA CGGCTGGCCT CAACAAGTGT TGACCATGCA GCGTAACTGG ATTGGTCGCT CTACTGGTAT GGAAATTACC TTCCCATCTG CCAATACTGA AATTTATGCT GACGGCTTAA CTGTTTATAC AACACGTGCC GACACACTTA TGGGGGTGAC GTACGTTGCC GTTGCAGCAG AACACCCTCT TGCGCTTAAA GCTGCTGAAA ATAACCCTGA ATTGGCTGCA TTTATTGAAG AATGCCGCAT GGGTTCAGTT GCAGAAGCTG ACTTGGCTAC AGCCGAGAAG AAAGGTATGG CAACAGGTTT GTTTGTAAAA CACCCTGTAA CTGGCGAAGA GCTTCCTGTC TGGATTGCGA ACTACGTATT AATGTCTTAC GGTTCTGGCG CAGTGATGGC TGTACCAGCA CATGACGAAC GTGACTTTGA ATTTGCTAAC AAGTTCAACT TGCCAATTAA GCAAGTGATT GATGCCAAAG GTGCTGACGA TGCAGATTAT TCTGCTACCG AGTGGCAAGA ATGGTACGGT TCTAAAGAAG GTAAACTTGT TAACTCTGGC GAGTTTGACG GTCTAGAATT CCAAGCTGCA TTCGATGCAT TCCTTGCGAA ATTAGAACCA CAAGGTTTAG CAAATTCTAA AGTTCAATTC CGTTTACGTG ACTGGGGTGT TTCTCGTCAA CGTTACTGGG GTTGTCCAAT TCCAATGATT AACTGTGATA CTTGTGGTCA AGTCACAGTT CCTGAAGATC AACTTCCGGT TGTATTACCA ACTGACGTTG TTCCAGATGG TTCAGGTAAT CCACTAAATA AAATGCCTGA ATTCTATGAA ACGAAATGTC CTTGCTGTGG TGGCGATGCG CGCCGTGAAA CAGATACATT GGACACATTC GTAGAATCAT CTTGGTACTA CGCACGCTAT GCATCTCCAG ACTTTACTGG TGGTATGGTA AAACCTGAAG CAGCTAAAAA CTGGCTTCCT GTAAACCAAT ATATTGGTGG TGTTGAACAC GCGATTCTTC ACTTACTTTA TGCACGTTTC TTCCACAAAT TAATGCGTGA TGAAGGTGTT GTACAAGGTA ATGAGCCATT TACCAACTTG TTAACTCAAG GCATGGTGCT TGCAGATACT TTCTATCGTG AAGCAGAAAA TGGTAAGAAA ACTTGGTTCA ACCCTGCTGA CATCGAATTA GAACGTGATG AGAAAGGCCG CATCATTTCT GCAAAATATT CAGGTGATGG TCAAGAAGTG ATTATCGGCG GACAAGAAAA AATGTCCAAG TCGAAAAATA ACGGTATTGA CCCACAAGCT ATTATTGATC AATACGGCGC AGATACAGCT CGTGTATTTA TGATGTTTGC TGCACCACCA GATCAATCTT TAGAATGGTC TGATGCAGGT GTTGAAGGTG CAAACCGTTT CTTGAAACGT GTATGGCGTC TGGTAGCAAG CTTCCTTGAA AAAGGAAACT CTGCAACAGC AATCGATAAA GCAAACTTGT CTAAAGATGC TCAAGATTTA CGTCGTAAGA CACACGAAAC CATTCAAAAA GTAAGTGATG ATATTGAGCG TCGTCATGCG TTCAATACTG CAATTGCTGC ATTGATGGAA TTGTTAAATG CAAGCAATAA GTTTGAAGCA AAAGATGACA ATGACGTTGC TGTAGAACGT GAAGCAATCA CCACATTGTT AACTTTACTT GCTCCATTTG CACCACATTT AAGCCAGACT TTATTGGCTC AGTTTGGTAC GGACTTAACA GAAGCAACTT TCCCTGAAGT TGATGCTTCT GCATTAACCC GCAATACTCA AACGATTGTT GTACAAGTAA ATGGTAAACT TCGCGGTAAA CTTGAAGTTT CTGTTGATAT TTCTAAAGAT GAGTTATTAG CTCAAGCGAA AGCATTACCA GAAGTTCAGC AGTTCTTAAC AGGACCAACC AAAAAAGAGA TTGTGGTTCC AAACAAATTG GTTAACCTCG TGGTTTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT