AcbaC.17941.a

tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase

CENTER ID: AcbaC.17941.a
ORGANISM: Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AcbaC.17941.a.B1.GE38842 full length 1 377
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|470.1345.peg.1089
GenBank: AKA30876.1
PGAT: acinetobacter:ABUW_1124
RefSeq: WP_001187604.1
UniProt: A0A081H1H1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQQRVIVGMS GGVDSSVSAA LLLQQGYQVE GLFMKNWEED DGTEYCTAME DLADAQAVAD KIGIKLHTAN FAMEYWDRVF EHFLAEYAAG RTPNPDILCN KEIKFRAFLD HAMTLGADFI ATGHYARRAE TAYNSKGEAY APLLRGLDNN KDQTYFLHAV HGREINKTLF PVGEIEKPEV RRIAEELDLA TAKKKDSTGI CFIGERRFND FLKQYLPAQP GKIVLDNGKE VGEHHGLMYY TLGQRGGIGL GGMKGASEGA WFVLHKDVAN NRLVVGQGHD HPLMQSTQLW SEAIDWVAGE QNIPAEGLRC TAKTRYRQPD QACTVFIDEN SEHGVRVEFD EPQRAVTPGQ SVVFYSDEVC LGGGVIHHTN APTPNFI
NT Sequence
ATGCAACAAC GTGTCATCGT CGGTATGTCT GGTGGCGTAG ATTCCTCTGT TTCTGCGGCG CTCTTACTTC AACAGGGTTA TCAAGTTGAA GGCCTTTTCA TGAAAAACTG GGAGGAAGAT GACGGCACGG AATACTGTAC GGCAATGGAA GATTTAGCCG ATGCTCAAGC AGTCGCAGAT AAAATCGGTA TTAAACTTCA TACTGCAAAC TTTGCTATGG AATATTGGGA CCGCGTATTC GAACACTTCT TGGCTGAATA TGCAGCCGGT CGCACTCCAA ATCCAGATAT CTTATGCAAT AAAGAAATTA AGTTTCGCGC ATTTTTAGAT CATGCCATGA CCTTAGGTGC AGATTTTATT GCAACAGGTC ATTATGCTCG TCGTGCTGAA ACTGCTTATA ACTCTAAAGG TGAGGCATAT GCACCTTTAT TACGTGGTTT AGATAACAAT AAAGACCAAA CTTATTTCTT ACATGCAGTG CATGGCCGTG AAATTAATAA AACCCTCTTC CCTGTAGGTG AAATTGAAAA ACCGGAAGTT CGTAGAATTG CTGAAGAACT GGACTTAGCA ACTGCGAAGA AAAAAGATTC AACTGGTATA TGTTTTATCG GTGAACGTCG CTTTAATGAC TTCCTTAAAC AATATTTACC CGCTCAACCA GGTAAAATTG TACTTGATAA CGGCAAAGAA GTTGGTGAAC ATCACGGTCT GATGTACTAT ACGCTCGGTC AACGTGGCGG TATTGGTCTA GGCGGTATGA AAGGTGCATC AGAAGGTGCA TGGTTTGTAC TTCATAAAGA TGTTGCCAAT AACCGTTTAG TGGTCGGCCA AGGACATGAT CACCCACTTA TGCAAAGTAC ACAGCTTTGG AGTGAGGCCA TTGACTGGGT GGCAGGCGAG CAAAATATTC CGGCTGAAGG ATTACGTTGC ACTGCTAAAA CACGCTATCG CCAGCCTGAT CAAGCTTGTA CAGTGTTTAT TGATGAAAAT AGTGAGCATG GTGTTCGTGT TGAGTTTGAT GAACCTCAAC GTGCAGTTAC ACCAGGCCAA AGTGTTGTAT TTTATTCGGA TGAAGTTTGC CTAGGCGGGG GTGTTATTCA CCATACAAAC GCCCCTACAC CAAATTTTAT TTAA
Details for AcbaC.17941.a.B1.GE38842
HARVESTED ON: 1/29/2015
SEQUENCED ON: 1/15/2015
EXPECTED MW: 43kDa
OBSERVED MW: 47kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHMQQ RVIVGMSGGV DSSVSAALLL QQGYQVEGLF MKNWEEDDGT EYCTAMEDLA DAQAVADKIG IKLHTANFAM EYWDRVFEHF LAEYAAGRTP NPDILCNKEI KFRAFLDHAM TLGADFIATG HYARRAETAY NSKGEAYAPL LRGLDNNKDQ TYFLHAVHGR EINKTLFPVG EIEKPEVRRI AEELDLATAK KKDSTGICFI GERRFNDFLK QYLPAQPGKI VLDNGKEVGE HHGLMYYTLG QRGGIGLGGM KGASEGAWFV LHKDVANNRL VVGQGHDHPL MQSTQLWSEA IDWVAGEQNI PAEGLRCTAK TRYRQPDQAC TVFIDENSEH GVRVEFDEPQ RAVTPGQSVV FYSDEVCLGG GVIHHT
Validated NT Sequence
gtttgtatgg tgaataacac ccccgcctag gcaaacttca tccgaataaa atacaacact ttggcctggt gtaactgcac gttgaggttc atcaaactca acacgaacac catgctcact attttcatca ataaacactg tacaagcttg atcaggctgg cgatagcgtg ttttagcagt gcaacgtaat ccttcagccg gaatattttg ctcgcctgcc acccagtcaa tggcctcact ccaaagctgt gtactttgca taagtgggtg atcatgtcct tggccgacca ctaaacggtt attggcaaca tctttatgaa gtacaaacca tgcaccttct gatgcacctt tcataccgcc tagaccaata ccgccacgtt gaccgagcgt atagtacatc agaccgtgat gttcaccaac ttctttgccg ttatcaagta caattttacc tggttgagcg ggtaaatatt gtttaaggaa gtcattaaag cgacgttcac cgataaaaca tataccagtt gaatcttttt tcttcgcagt tgctaagtcc agttcttcag caattctacg aacttccggt ttttcaattt cacctacagg gaagagggtt ttattaattt cacggccatg cactgcatgt aagaaataag tttggtcttt attgttatct aaaccacgta ataaaggtgc atatgcctca cctttagagt tataagcagt ttcagcacga cgagcataat gacctgttgc aataaaatct gcacctaagg tcatggcatg atctaaaaat gcgcgaaact taatttcttt attgcataag atatctggat ttggagtgcg accggctgca tattcagcca agaagtgttc gaatacgcgg tcccaatatt ccatagcaaa gtttgcagta tgaagtttaa taccgatttt atctgcgact gcttgagcat cggctaaatc ttccattgcc gtacagtatt ccgtgccgtc atcttcctcc cagtttttca tgaaaaggcc ttcaacttga taaccctgtt gaagtaagag cgccgcagaa acagaggaat ctacgccacc agacataccg acgatgacac gttgttgcat atggtggtgg tggtgagcca t
Expected Protein Sequence
MAHHHHHHMQ QRVIVGMSGG VDSSVSAALL LQQGYQVEGL FMKNWEEDDG TEYCTAMEDL ADAQAVADKI GIKLHTANFA MEYWDRVFEH FLAEYAAGRT PNPDILCNKE IKFRAFLDHA MTLGADFIAT GHYARRAETA YNSKGEAYAP LLRGLDNNKD QTYFLHAVHG REINKTLFPV GEIEKPEVRR IAEELDLATA KKKDSTGICF IGERRFNDFL KQYLPAQPGK IVLDNGKEVG EHHGLMYYTL GQRGGIGLGG MKGASEGAWF VLHKDVANNR LVVGQGHDHP LMQSTQLWSE AIDWVAGEQN IPAEGLRCTA KTRYRQPDQA CTVFIDENSE HGVRVEFDEP QRAVTPGQSV VFYSDEVCLG GGVIHHTNAP TPNFI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcaac aacgtgtcat cgtcggtatg tctggtggcg tagattcctc tgtttctgcg gcgctcttac ttcaacaggg ttatcaagtt gaaggccttt tcatgaaaaa ctgggaggaa gatgacggca cggaatactg tacggcaatg gaagatttag ccgatgctca agcagtcgca gataaaatcg gtattaaact tcatactgca aactttgcta tggaatattg ggaccgcgta ttcgaacact tcttggctga atatgcagcc ggtcgcactc caaatccaga tatcttatgc aataaagaaa ttaagtttcg cgcattttta gatcatgcca tgaccttagg tgcagatttt attgcaacag gtcattatgc tcgtcgtgct gaaactgctt ataactctaa aggtgaggca tatgcacctt tattacgtgg tttagataac aataaagacc aaacttattt cttacatgca gtgcatggcc gtgaaattaa taaaaccctc ttccctgtag gtgaaattga aaaaccggaa gttcgtagaa ttgctgaaga actggactta gcaactgcga agaaaaaaga ttcaactggt atatgtttta tcggtgaacg tcgctttaat gacttcctta aacaatattt acccgctcaa ccaggtaaaa ttgtacttga taacggcaaa gaagttggtg aacatcacgg tctgatgtac tatacgctcg gtcaacgtgg cggtattggt ctaggcggta tgaaaggtgc atcagaaggt gcatggtttg tacttcataa agatgttgcc aataaccgtt tagtggtcgg ccaaggacat gatcacccac ttatgcaaag tacacagctt tggagtgagg ccattgactg ggtggcaggc gagcaaaata ttccggctga aggattacgt tgcactgcta aaacacgcta tcgccagcct gatcaagctt gtacagtgtt tattgatgaa aatagtgagc atggtgttcg tgttgagttt gatgaacctc aacgtgcagt tacaccaggc caaagtgttg tattttattc ggatgaagtt tgcctaggcg ggggtgttat tcaccataca aacgccccta caccaaattt tatttgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat