AcbaC.17938.a
UDP-N-acetylmuramoylalanine--D-glutamate ligase (MurD)
| CENTER ID: | AcbaC.17938.a |
| ORGANISM: | Acinetobacter baumannii AB5075-UW |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| AcbaC.17938.a.B1.GE38840 | full length | 1 | 448 | ||
| AcbaC.17938.a.B2.GE40450 | truncation1 | 10 | 446 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| AcbaC.17938.a.B2.PW37960 | truncation1 | 10 | 446 | ||
| AcbaC.17938.a.B1.PW39235 | full length | 1 | 448 |
Structures
7SIR
DEPOSITED: 10/14/2021
DETERMINATION: XRay
CLONE: AcbaC.17938.a.B1.GE38840
PROTEIN: AcbaC.17938.a.B1.PS02380
7TI7
DEPOSITED: 1/12/2022
DETERMINATION: XRay
CLONE: AcbaC.17938.a.B1.GE38840
PROTEIN: AcbaC.17938.a.B1.PS02380
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| GenBank: | AKA33315.1 |
| PATRIC ID: | fig|470.1345.peg.3557 |
| PGAT: | acinetobacter:ABUW_3630 |
| RefSeq: | WP_000908240.1 |
| UniProt: | A0A0B9W6J3 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLIQRGGLKV VAGLGISGVS AVNFLHEQGY QVAVTDSRPT PPGHDQIPAG VKTSFGQLDQ ELLLQAEEII LSPGLAPQLP EIQAAIAKGI SVVGDIQLLR RATDVPIVAI TGSNAKSTVT TLIGLMAKDA GKKVAVGGNL GRPALDLLKD QPELLVLELS SFQLETTSHL NAEVAVVLNM SEDHLDRHGN MLGYHQAKHR IFQGAKKVVF NRDDALSRPL VPDTTPMQSF GLNAPDLNQY GVLRDADGTL WLARGLQRLI KSSDLYIQGM HNVANALACL ALGEAIGLPM ESMLETLKQF KGLEHRCEYV KTVHDVRYYN DSKGTNVGAT LAAIDGLGAA IEVKKGKVAL ILGGQGKGQD FGPLRSSIEK YAKVVVLIGE DAPVIEQAIQ GATKILHAAT LKEAVELCQR ETQAEDVVLL SPACASFDMF KSYNDRGQQF VACVNSLV
NT Sequence
ATGTTAATAC AACGTGGTGG ATTAAAAGTT GTCGCAGGCT TGGGAATATC AGGTGTTTCT GCTGTAAATT TCCTGCATGA ACAAGGCTAC CAAGTTGCTG TAACGGATTC CCGCCCCACA CCTCCCGGAC ACGATCAGAT TCCGGCTGGC GTTAAAACCA GTTTTGGTCA GCTTGATCAA GAATTATTAT TACAAGCAGA AGAGATTATT TTAAGCCCAG GCCTTGCACC GCAATTACCG GAAATTCAGG CAGCTATTGC TAAGGGTATT TCTGTGGTGG GCGATATCCA GTTATTGCGC CGTGCCACTG ATGTGCCGAT TGTAGCGATT ACAGGTTCCA ATGCAAAAAG TACTGTAACG ACTTTAATTG GCTTAATGGC TAAGGATGCA GGAAAGAAAG TTGCAGTAGG CGGCAATCTT GGTCGACCAG CGTTAGATTT ACTTAAAGAT CAACCAGAGT TACTGGTGCT TGAGTTATCA AGCTTTCAGT TAGAAACCAC CTCTCACTTA AACGCTGAGG TAGCAGTGGT TCTCAATATG AGTGAAGACC ATTTAGACCG TCATGGAAAT ATGCTGGGGT ATCACCAAGC AAAACATCGT ATTTTCCAAG GCGCTAAAAA AGTTGTATTT AACCGAGACG ATGCGTTAAG CCGCCCACTT GTTCCTGATA CGACACCAAT GCAAAGCTTT GGTTTAAACG CACCGGATTT AAATCAATAT GGCGTTTTAA GAGATGCCGA CGGTACGCTT TGGCTTGCTC GTGGTTTACA GCGGTTAATT AAAAGCTCGG ATTTATATAT TCAAGGTATG CACAACGTAG CCAATGCTTT AGCGTGTTTA GCATTGGGGG AGGCAATTGG TTTACCTATG GAGTCAATGC TTGAAACTTT AAAACAATTT AAAGGATTAG AGCACCGCTG TGAGTACGTT AAAACCGTGC ATGATGTCCG TTATTATAAT GACTCTAAAG GTACCAATGT TGGTGCTACA CTTGCAGCTA TTGATGGCTT AGGCGCTGCC ATTGAAGTGA AAAAAGGTAA GGTTGCACTT ATTTTAGGTG GACAGGGCAA AGGACAAGAT TTTGGTCCAT TACGTTCTTC TATCGAAAAA TATGCCAAAG TTGTGGTATT GATTGGTGAA GATGCGCCTG TCATCGAACA AGCCATTCAA GGCGCAACTA AAATTTTACA TGCAGCAACG CTTAAAGAAG CTGTCGAGCT GTGTCAACGT GAAACACAAG CTGAAGATGT GGTATTGCTA TCACCAGCAT GTGCAAGTTT TGATATGTTT AAAAGTTATA ATGACCGTGG TCAGCAGTTT GTTGCCTGCG TCAATTCGTT GGTTTAA
Details for AcbaC.17938.a.B1.GE38840
| HARVESTED ON: | 1/29/2015 |
| SEQUENCED ON: | 1/15/2015 |
| EXPECTED MW: | 49kDa |
| OBSERVED MW: | 47kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHML IQRGGLKVVA GLGISGVSAV NFLHEQGYQV AVTDSRPTPP GHDQIPAGVK TSFGQLDQEL LLQAEEIILS PGLAPQLPEI QAAIAKGISV VGDIQLLRRA TDVPIVAITG SNAKSTVTTL IGLMAKDAGK KVAVGGNLGR PALDLLKDQP ELLVLELSSF QLETTSHLNA EVAVVLNMSE DHLDRHGNML GYHQAKHRIF QGAKKVVFNR DDALSRPLVP DTTPMQSFGL NAPDLNQYGV LRDADGTLWL ARGLQRLIKS SDLYIQGMHN VANALACLAL GEAIGLPMES MLETLKQFKG LEHRCEYVKT VHDVRYYNDS KGTNVGATLA AIDGLGAAIE VKKGKVALIL GGQGKGQDFG PLRSSIEKYA KVVVLIGEDA PVIEQAIQGA TKILHAATLK EAVELCQRET QAEDVVLLSP ACASFDMFKS YNDRGQQFVA CVNSLV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttaa tacaacgtgg tggattaaaa gttgtcgcag gcttgggaat atcaggtgtt tctgctgtaa atttcctgca tgaacaaggc taccaagttg ctgtaacgga ttcccgcccc acacctcccg gacacgatca gattccggct ggcgttaaaa ccagttttgg tcagcttgat caagaattat tattacaagc agaagagatt attttaagcc caggccttgc accgcaatta ccggaaattc aggcagctat tgctaagggt atttctgtgg tgggcgatat ccagttattg cgccgtgcca ctgatgtgcc gattgtagcg attacaggtt ccaatgcaaa aagtactgta acgactttaa ttggcttaat ggctaaggat gcaggaaaga aagttgcagt aggcggcaat cttggtcgac cagcgttaga tttacttaaa gatcaaccag agttactggt gcttgagtta tcaagctttc agttagaaac cacctctcac ttaaacgctg aggtagcagt ggttctcaat atgagtgaag accatttaga ccgtcatgga aatatgctgg ggtatcacca agcaaaacat cgtattttcc aaggcgctaa aaaagttgta tttaaccgag acgatgcgtt aagccgccca cttgttcctg atacgacacc aatgcaaagc tttggtttaa acgcaccgga tttaaatcaa tatggcgttt taagagatgc cgacggtacg ctttggcttg ctcgtggttt acagcggtta attaaaagct cggatttata tattcaaggt atgcacaacg tagccaatgc tttagcgtgt ttagcattgg gggaggcaat tggtttacct atggagtcaa tgcttgaaac tttaaaacaa tttaaaggat tagagcaccg ctgtgagtac gttaaaaccg tgcatgatgt ccgttattat aatgactcta aaggtaccaa tgttggtgct acacttgcag ctattgatgg cttaggcgct gccattgaag tgaaaaaagg taaggttgca cttattttag gtggacaggg caaaggacaa gattttggtc cattacgttc ttctatcgaa aaatatgcca aagttgtggt attgattggt gaagatgcgc ctgtcatcga acaagccatt caaggcgcaa ctaaaatttt acatgcagca acgcttaaag aagctgtcga gctgtgtcaa cgtgaaacac aagctgaaga tgtggtattg ctatcaccag catgtgcaag ttttgatatg tttaaaagtt ataatgaccg tggtcagcag tttgttgcct gcgtcaattc gttggtttga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for AcbaC.17938.a.B2.GE40450
| HARVESTED ON: | 8/10/2016 |
| SEQUENCED ON: | 8/11/2016 |
| EXPECTED MW: | 48kDa |
| OBSERVED MW: | 45kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHVV AGLGISGVSA VNFLHEQGYQ VAVTDSRPTP PGHDQIPAGV KTSFGQLDQE LLLQAEEIIL SPGLAPQLPE IQAAIAKGIS VVGDIQLLRR ATDVPIVAIT GSNAKSTVTT LIGLMAKDAG KKVAVGGNLG RPALDLLKDQ PELLVLELSS FQLETTSHLN AEVAVVLNMS EDHLDRHGNM LGYHQAKHRI FQGAKKVVFN RDDALSRPLV PDTTPMQSFG LNAPDLNQYG VLRDADGTLW LARGLQRLIK SSDLYIQGMH NVANALACLA LGEAIGLPME SMLETLKQFK GLEHRCEYVK TVHDVRYYND SKGTNVGATL AAIDGLGAAI EVKKGKVALI LGGQGKGQDF GPLRSSIEKY AKVVVLIGED APVIEQAIQG ATKILHAATL KEAVELCQRE TQAEDVVLLS PACASFDMFK SYNDRGQQFV ACVNS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgttgtcg caggcttggg aatatcaggt gtttctgctg taaatttcct gcatgaacaa ggctaccaag ttgctgtaac ggattcccgc cccacacctc ccggacacga tcagattccg gctggcgtta aaaccagttt tggtcagctt gatcaagaat tattattaca agcagaagag attattttaa gcccaggcct tgcaccgcaa ttaccggaaa ttcaggcagc tattgctaag ggtatttctg tggtgggcga tatccagtta ttgcgccgtg ccactgatgt gccgattgta gcgattacag gttccaatgc aaaaagtact gtaacgactt taattggctt aatggctaag gatgcaggaa agaaagttgc agtaggcggc aatcttggtc gaccagcgtt agatttactt aaagatcaac cagagttact ggtgcttgag ttatcaagct ttcagttaga aaccacctct cacttaaacg ctgaggtagc agtggttctc aatatgagtg aagaccattt agaccgtcat ggaaatatgc tggggtatca ccaagcaaaa catcgtattt tccaaggcgc taaaaaagtt gtatttaacc gagacgatgc gttaagccgc ccacttgttc ctgatacgac accaatgcaa agctttggtt taaacgcacc ggatttaaat caatatggcg ttttaagaga tgccgacggt acgctttggc ttgctcgtgg tttacagcgg ttaattaaaa gctcggattt atatattcaa ggtatgcaca acgtagccaa tgctttagcg tgtttagcat tgggggaggc aattggttta cctatggagt caatgcttga aactttaaaa caatttaaag gattagagca ccgctgtgag tacgttaaaa ccgtgcatga tgtccgttat tataatgact ctaaaggtac caatgttggt gctacacttg cagctattga tggcttaggc gctgccattg aagtgaaaaa aggtaaggtt gcacttattt taggtggaca gggcaaagga caagattttg gtccattacg ttcttctatc gaaaaatatg ccaaagttgt ggtattgatt ggtgaagatg cgcctgtcat cgaacaagcc attcaaggcg caactaaaat tttacatgca gcaacgctta aagaagctgt cgagctgtgt caacgtgaaa cacaagctga agatgtggta ttgctatcac cagcatgtgc aagttttgat atgtttaaaa gttataatga ccgtggtcag cagtttgttg cctgcgtcaa ttcgtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for AcbaC.17938.a.B2.PW37960
| PURIFICATION DATe: | 11/1/2016 |
| CONCENTRATION: | 43mg/ml |
| OBSERVED MW: | 45kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 8 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHVV AGLGISGVSA VNFLHEQGYQ VAVTDSRPTP PGHDQIPAGV KTSFGQLDQE LLLQAEEIIL SPGLAPQLPE IQAAIAKGIS VVGDIQLLRR ATDVPIVAIT GSNAKSTVTT LIGLMAKDAG KKVAVGGNLG RPALDLLKDQ PELLVLELSS FQLETTSHLN AEVAVVLNMS EDHLDRHGNM LGYHQAKHRI FQGAKKVVFN RDDALSRPLV PDTTPMQSFG LNAPDLNQYG VLRDADGTLW LARGLQRLIK SSDLYIQGMH NVANALACLA LGEAIGLPME SMLETLKQFK GLEHRCEYVK TVHDVRYYND SKGTNVGATL AAIDGLGAAI EVKKGKVALI LGGQGKGQDF GPLRSSIEKY AKVVVLIGED APVIEQAIQG ATKILHAATL KEAVELCQRE TQAEDVVLLS PACASFDMFK SYNDRGQQFV ACVNS
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGTTGTCG CAGGCTTGGG AATATCAGGT GTTTCTGCTG TAAATTTCCT GCATGAACAA GGCTACCAAG TTGCTGTAAC GGATTCCCGC CCCACACCTC CCGGACACGA TCAGATTCCG GCTGGCGTTA AAACCAGTTT TGGTCAGCTT GATCAAGAAT TATTATTACA AGCAGAAGAG ATTATTTTAA GCCCAGGCCT TGCACCGCAA TTACCGGAAA TTCAGGCAGC TATTGCTAAG GGTATTTCTG TGGTGGGCGA TATCCAGTTA TTGCGCCGTG CCACTGATGT GCCGATTGTA GCGATTACAG GTTCCAATGC AAAAAGTACT GTAACGACTT TAATTGGCTT AATGGCTAAG GATGCAGGAA AGAAAGTTGC AGTAGGCGGC AATCTTGGTC GACCAGCGTT AGATTTACTT AAAGATCAAC CAGAGTTACT GGTGCTTGAG TTATCAAGCT TTCAGTTAGA AACCACCTCT CACTTAAACG CTGAGGTAGC AGTGGTTCTC AATATGAGTG AAGACCATTT AGACCGTCAT GGAAATATGC TGGGGTATCA CCAAGCAAAA CATCGTATTT TCCAAGGCGC TAAAAAAGTT GTATTTAACC GAGACGATGC GTTAAGCCGC CCACTTGTTC CTGATACGAC ACCAATGCAA AGCTTTGGTT TAAACGCACC GGATTTAAAT CAATATGGCG TTTTAAGAGA TGCCGACGGT ACGCTTTGGC TTGCTCGTGG TTTACAGCGG TTAATTAAAA GCTCGGATTT ATATATTCAA GGTATGCACA ACGTAGCCAA TGCTTTAGCG TGTTTAGCAT TGGGGGAGGC AATTGGTTTA CCTATGGAGT CAATGCTTGA AACTTTAAAA CAATTTAAAG GATTAGAGCA CCGCTGTGAG TACGTTAAAA CCGTGCATGA TGTCCGTTAT TATAATGACT CTAAAGGTAC CAATGTTGGT GCTACACTTG CAGCTATTGA TGGCTTAGGC GCTGCCATTG AAGTGAAAAA AGGTAAGGTT GCACTTATTT TAGGTGGACA GGGCAAAGGA CAAGATTTTG GTCCATTACG TTCTTCTATC GAAAAATATG CCAAAGTTGT GGTATTGATT GGTGAAGATG CGCCTGTCAT CGAACAAGCC ATTCAAGGCG CAACTAAAAT TTTACATGCA GCAACGCTTA AAGAAGCTGT CGAGCTGTGT CAACGTGAAA CACAAGCTGA AGATGTGGTA TTGCTATCAC CAGCATGTGC AAGTTTTGAT ATGTTTAAAA GTTATAATGA CCGTGGTCAG CAGTTTGTTG CCTGCGTCAA TTCGTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT
Details for AcbaC.17938.a.B1.PW39235
| PURIFICATION DATe: | 9/6/2023 |
| CONCENTRATION: | 28.7mg/ml |
| OBSERVED MW: | 49kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 34 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHML IQRGGLKVVA GLGISGVSAV NFLHEQGYQV AVTDSRPTPP GHDQIPAGVK TSFGQLDQEL LLQAEEIILS PGLAPQLPEI QAAIAKGISV VGDIQLLRRA TDVPIVAITG SNAKSTVTTL IGLMAKDAGK KVAVGGNLGR PALDLLKDQP ELLVLELSSF QLETTSHLNA EVAVVLNMSE DHLDRHGNML GYHQAKHRIF QGAKKVVFNR DDALSRPLVP DTTPMQSFGL NAPDLNQYGV LRDADGTLWL ARGLQRLIKS SDLYIQGMHN VANALACLAL GEAIGLPMES MLETLKQFKG LEHRCEYVKT VHDVRYYNDS KGTNVGATLA AIDGLGAAIE VKKGKVALIL GGQGKGQDFG PLRSSIEKYA KVVVLIGEDA PVIEQAIQGA TKILHAATLK EAVELCQRET QAEDVVLLSP ACASFDMFKS YNDRGQQFVA CVNSLV
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTTAA TACAACGTGG TGGATTAAAA GTTGTCGCAG GCTTGGGAAT ATCAGGTGTT TCTGCTGTAA ATTTCCTGCA TGAACAAGGC TACCAAGTTG CTGTAACGGA TTCCCGCCCC ACACCTCCCG GACACGATCA GATTCCGGCT GGCGTTAAAA CCAGTTTTGG TCAGCTTGAT CAAGAATTAT TATTACAAGC AGAAGAGATT ATTTTAAGCC CAGGCCTTGC ACCGCAATTA CCGGAAATTC AGGCAGCTAT TGCTAAGGGT ATTTCTGTGG TGGGCGATAT CCAGTTATTG CGCCGTGCCA CTGATGTGCC GATTGTAGCG ATTACAGGTT CCAATGCAAA AAGTACTGTA ACGACTTTAA TTGGCTTAAT GGCTAAGGAT GCAGGAAAGA AAGTTGCAGT AGGCGGCAAT CTTGGTCGAC CAGCGTTAGA TTTACTTAAA GATCAACCAG AGTTACTGGT GCTTGAGTTA TCAAGCTTTC AGTTAGAAAC CACCTCTCAC TTAAACGCTG AGGTAGCAGT GGTTCTCAAT ATGAGTGAAG ACCATTTAGA CCGTCATGGA AATATGCTGG GGTATCACCA AGCAAAACAT CGTATTTTCC AAGGCGCTAA AAAAGTTGTA TTTAACCGAG ACGATGCGTT AAGCCGCCCA CTTGTTCCTG ATACGACACC AATGCAAAGC TTTGGTTTAA ACGCACCGGA TTTAAATCAA TATGGCGTTT TAAGAGATGC CGACGGTACG CTTTGGCTTG CTCGTGGTTT ACAGCGGTTA ATTAAAAGCT CGGATTTATA TATTCAAGGT ATGCACAACG TAGCCAATGC TTTAGCGTGT TTAGCATTGG GGGAGGCAAT TGGTTTACCT ATGGAGTCAA TGCTTGAAAC TTTAAAACAA TTTAAAGGAT TAGAGCACCG CTGTGAGTAC GTTAAAACCG TGCATGATGT CCGTTATTAT AATGACTCTA AAGGTACCAA TGTTGGTGCT ACACTTGCAG CTATTGATGG CTTAGGCGCT GCCATTGAAG TGAAAAAAGG TAAGGTTGCA CTTATTTTAG GTGGACAGGG CAAAGGACAA GATTTTGGTC CATTACGTTC TTCTATCGAA AAATATGCCA AAGTTGTGGT ATTGATTGGT GAAGATGCGC CTGTCATCGA ACAAGCCATT CAAGGCGCAA CTAAAATTTT ACATGCAGCA ACGCTTAAAG AAGCTGTCGA GCTGTGTCAA CGTGAAACAC AAGCTGAAGA TGTGGTATTG CTATCACCAG CATGTGCAAG TTTTGATATG TTTAAAAGTT ATAATGACCG TGGTCAGCAG TTTGTTGCCT GCGTCAATTC GTTGGTTTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT