AcbaC.17935.a

chaperonin GroL

CENTER ID:	AcbaC.17935.a
ORGANISM:	Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AcbaC.17935.a.B1.GE38838	full length		1	544

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AcbaC.17935.a.B1.PS02461	full length		1	544

External Resources

RESOURCE	REFERENCE ID
GenBank:	AKA30675.1
PATRIC ID:	fig\|470.1345.peg.883
PGAT:	acinetobacter:ABUW_0918
RefSeq:	WP_001274623.1
UniProt:	V5VAH2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSAKDVKFGD SARSKMIAGV NVLADAVKVT LGPKGRNVVI DRSFGAPHIT KDGVTVAKEI SLKDKFENMG AQLVREVSSK TNDIAGDGTT TATVLAQAIL NEGIKSVTAG MNPMDLKRGI DIAVKTVVEN IRSIAKPADD FKAIEQVGSI SANSDTTVGK LIAQAMEKVG KEGVITVEEG SGFEDALDVV EGMQFDRGYI SPYFANKQDT LTAELENPFI LLVDKKISNI RELISVLEAV AKTGKPLLII AEDVEGEALA TLVVNNMRGI IKVCAVKAPG FGDRRKAMLQ DIAILTGATV ISEEVGMSLE QATLQDLGTA HKITVSKENT VIVDGAGDAA AIAERVQQIR AQIEESTSEY DREKLQERVA KLAGGVAVIK IGAATEVEMK EKKDRVDDAL HATRAAVEEG VVAGGGVALV RAVNALEGLK GANEDQTAGI NILRRAIEAP LRQIVANAGD EPSVVINAVK NGEGNFGYNA ATGEYGDMLE MGILDPAKVT RSALEHAASV AGLMLTTECM ITDIPEDKPA APDMGGMGGM GGMM

NT Sequence

ATGTCAGCTA AAGACGTAAA ATTTGGTGAT TCAGCTCGCT CAAAAATGAT TGCAGGGGTA AACGTACTTG CAGATGCGGT TAAAGTGACT TTAGGTCCTA AAGGCCGTAA TGTTGTGATC GACCGCTCTT TCGGCGCGCC GCACATCACT AAAGACGGTG TAACTGTTGC AAAAGAAATT TCATTAAAAG ACAAGTTTGA AAACATGGGT GCTCAACTTG TTCGTGAAGT TTCAAGCAAA ACTAACGACA TCGCAGGTGA CGGTACAACA ACTGCAACTG TACTTGCTCA AGCAATTTTA AATGAAGGTA TCAAATCAGT AACTGCTGGT ATGAACCCAA TGGATTTAAA ACGCGGTATC GACATTGCAG TAAAAACTGT AGTTGAAAAT ATCCGTTCTA TTGCTAAACC AGCTGATGAT TTCAAAGCAA TTGAACAAGT AGGTTCAATC TCTGCTAACT CTGATACTAC TGTTGGTAAA CTTATTGCTC AAGCAATGGA AAAAGTAGGT AAAGAAGGCG TAATCACTGT AGAAGAAGGT TCTGGCTTCG AAGACGCATT AGACGTTGTA GAAGGTATGC AGTTTGACCG TGGTTATATC TCTCCGTACT TTGCAAACAA ACAAGATACT TTAACTGCTG AACTTGAAAA TCCGTTCATT CTTCTTGTTG ATAAAAAAAT CAGCAACATT CGTGAATTGA TTTCTGTTTT AGAAGCAGTT GCTAAAACTG GTAAACCACT TCTTATCATC GCTGAAGATG TTGAAGGTGA AGCGCTTGCA ACTCTTGTTG TAAACAACAT GCGCGGTATC ATCAAAGTAT GTGCTGTTAA AGCTCCTGGT TTCGGTGACC GTCGTAAAGC AATGCTTCAA GATATCGCGA TCTTGACTGG CGCAACAGTT ATTTCTGAAG AAGTTGGTAT GTCTTTAGAA CAAGCAACTC TTCAAGATTT AGGTACAGCG CACAAAATCA CTGTTTCTAA AGAAAACACA GTTATTGTTG ACGGTGCTGG TGATGCTGCT GCTATCGCTG AGCGTGTTCA ACAAATCCGT GCTCAAATCG AAGAGTCTAC TTCAGAATAT GACCGTGAAA AATTACAAGA ACGTGTTGCT AAGTTAGCAG GCGGTGTTGC TGTAATTAAA ATCGGTGCAG CAACTGAAGT TGAAATGAAA GAGAAGAAAG ACCGCGTAGA CGACGCACTT CACGCAACTC GTGCAGCAGT TGAAGAAGGT GTTGTTGCTG GTGGTGGTGT TGCTCTTGTT CGCGCTGTAA ATGCTCTTGA AGGCTTAAAA GGCGCTAACG AAGATCAAAC AGCTGGTATC AACATTTTAC GCCGTGCGAT CGAAGCTCCA CTTCGTCAAA TCGTTGCGAA TGCTGGTGAT GAGCCATCTG TAGTTATCAA CGCTGTTAAG AATGGTGAAG GTAACTTCGG TTACAACGCT GCAACTGGCG AATATGGTGA TATGTTAGAA ATGGGTATCC TTGACCCAGC TAAAGTAACT CGTTCTGCAC TTGAGCACGC TGCTTCTGTT GCTGGCTTAA TGTTAACTAC AGAATGTATG ATTACTGACA TTCCTGAAGA CAAACCAGCT GCTCCAGATA TGGGCGGTAT GGGTGGTATG GGCGGAATGA TGTAA

Details for AcbaC.17935.a.B1.GE38838

HARVESTED ON:	1/29/2015
SEQUENCED ON:	1/15/2015
EXPECTED MW:	58kDa
OBSERVED MW:	58kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMS AKDVKFGDSA RSKMIAGVNV LADAVKVTLG PKGRNVVIDR SFGAPHITKD GVTVAKEISL KDKFENMGAQ LVREVSSKTN DIAGDGTTTA TVLAQAILNE GIKSVTAGMN PMDLKRGIDI AVKTVVENIR SIAKPADDFK AIEQVGSISA NSDTTVGKLI AQAMEKVGKE GVITVEEGSG FEDALDVVEG MQFDRGYISP YFANKQDTLT AELENPFILL VDKKISNIRE LISVLEAVAK TGKPLLIIAE DVEGEALATL VVNNMRGIIK VCAVKAPGFG DRRKAMLQDI AILTGATVIS EEVGMSLEQA TLQDLGTAHK ITVSKENTVI VDGAGDAAAI AERVQQIRAQ IEESTSEYDR EKLQERVAKL AGGVAVIKIG AATEVEMKEK KDRVDDALHA TRAAVEEGVV AGGGVALVRA VNALEGLKGA NEDQTAGINI LRRAIEAPLR QIVANAGDEP SVVINAVKNG EGNFGYNAAT GEYGDMLEMG ILDPAKVTRS ALEHAASVAG LMLTTECMIT DIPEDKPAAP DMGGMGGMGG MM

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcag ctaaagacgt aaaatttggt gattcagctc gctcaaaaat gattgcaggg gtaaacgtac ttgcagatgc ggttaaagtg actttaggtc ctaaaggccg taatgttgtg atcgaccgct ctttcggcgc gccgcacatc actaaagacg gtgtaactgt tgcaaaagaa atttcattaa aagacaagtt tgaaaacatg ggtgctcaac ttgttcgtga agtttcaagc aaaactaacg acatcgcagg tgacggtaca acaactgcaa ctgtacttgc tcaagcaatt ttaaatgaag gtatcaaatc agtaactgct ggtatgaacc caatggattt aaaacgcggt atcgacattg cagtaaaaac tgtagttgaa aatatccgtt ctattgctaa accagctgat gatttcaaag caattgaaca agtaggttca atctctgcta actctgatac tactgttggt aaacttattg ctcaagcaat ggaaaaagta ggtaaagaag gcgtaatcac tgtagaagaa ggttctggct tcgaagacgc attagacgtt gtagaaggta tgcagtttga ccgtggttat atctctccgt actttgcaaa caaacaagat actttaactg ctgaacttga aaatccgttc attcttcttg ttgataaaaa aatcagcaac attcgtgaat tgatttctgt tttagaagca gttgctaaaa ctggtaaacc acttcttatc atcgctgaag atgttgaagg tgaagcgctt gcaactcttg ttgtaaacaa catgcgcggt atcatcaaag tatgtgctgt taaagctcct ggtttcggtg accgtcgtaa agcaatgctt caagatatcg cgatcttgac tggcgcaaca gttatttctg aagaagttgg tatgtcttta gaacaagcaa ctcttcaaga tttaggtaca gcgcacaaaa tcactgtttc taaagaaaac acagttattg ttgacggtgc tggtgatgct gctgctatcg ctgagcgtgt tcaacaaatc cgtgctcaaa tcgaagagtc tacttcagaa tatgaccgtg aaaaattaca agaacgtgtt gctaagttag caggcggtgt tgctgtaatt aaaatcggtg cagcaactga agttgaaatg aaagagaaga aagaccgcgt agacgacgca cttcacgcaa ctcgtgcagc agttgaagaa ggtgttgttg ctggtggtgg tgttgctctt gttcgcgctg taaatgctct tgaaggctta aaaggcgcta acgaagatca aacagctggt atcaacattt tacgccgtgc gatcgaagct ccacttcgtc aaatcgttgc gaatgctggt gatgagccat ctgtagttat caacgctgtt aagaatggtg aaggtaactt cggttacaac gctgcaactg gcgaatatgg tgatatgtta gaaatgggta tccttgaccc agctaaagta actcgttctg cacttgagca cgctgcttct gttgctggct taatgttaac tacagaatgt atgattactg acattcctga agacaaacca gctgctccag atatgggcgg tatgggtggt atgggcggaa tgatgtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat

Details for AcbaC.17935.a.B1.PS02461

PURIFICATION DATe:	7/29/2015
CONCENTRATION:	29.37mg/ml
OBSERVED MW:	64kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	SEC buffer
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	3
VIAL VOLUME:	110µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCAG CTAAAGACGT AAAATTTGGT GATTCAGCTC GCTCAAAAAT GATTGCAGGG GTAAACGTAC TTGCAGATGC GGTTAAAGTG ACTTTAGGTC CTAAAGGCCG TAATGTTGTG ATCGACCGCT CTTTCGGCGC GCCGCACATC ACTAAAGACG GTGTAACTGT TGCAAAAGAA ATTTCATTAA AAGACAAGTT TGAAAACATG GGTGCTCAAC TTGTTCGTGA AGTTTCAAGC AAAACTAACG ACATCGCAGG TGACGGTACA ACAACTGCAA CTGTACTTGC TCAAGCAATT TTAAATGAAG GTATCAAATC AGTAACTGCT GGTATGAACC CAATGGATTT AAAACGCGGT ATCGACATTG CAGTAAAAAC TGTAGTTGAA AATATCCGTT CTATTGCTAA ACCAGCTGAT GATTTCAAAG CAATTGAACA AGTAGGTTCA ATCTCTGCTA ACTCTGATAC TACTGTTGGT AAACTTATTG CTCAAGCAAT GGAAAAAGTA GGTAAAGAAG GCGTAATCAC TGTAGAAGAA GGTTCTGGCT TCGAAGACGC ATTAGACGTT GTAGAAGGTA TGCAGTTTGA CCGTGGTTAT ATCTCTCCGT ACTTTGCAAA CAAACAAGAT ACTTTAACTG CTGAACTTGA AAATCCGTTC ATTCTTCTTG TTGATAAAAA AATCAGCAAC ATTCGTGAAT TGATTTCTGT TTTAGAAGCA GTTGCTAAAA CTGGTAAACC ACTTCTTATC ATCGCTGAAG ATGTTGAAGG TGAAGCGCTT GCAACTCTTG TTGTAAACAA CATGCGCGGT ATCATCAAAG TATGTGCTGT TAAAGCTCCT GGTTTCGGTG ACCGTCGTAA AGCAATGCTT CAAGATATCG CGATCTTGAC TGGCGCAACA GTTATTTCTG AAGAAGTTGG TATGTCTTTA GAACAAGCAA CTCTTCAAGA TTTAGGTACA GCGCACAAAA TCACTGTTTC TAAAGAAAAC ACAGTTATTG TTGACGGTGC TGGTGATGCT GCTGCTATCG CTGAGCGTGT TCAACAAATC CGTGCTCAAA TCGAAGAGTC TACTTCAGAA TATGACCGTG AAAAATTACA AGAACGTGTT GCTAAGTTAG CAGGCGGTGT TGCTGTAATT AAAATCGGTG CAGCAACTGA AGTTGAAATG AAAGAGAAGA AAGACCGCGT AGACGACGCA CTTCACGCAA CTCGTGCAGC AGTTGAAGAA GGTGTTGTTG CTGGTGGTGG TGTTGCTCTT GTTCGCGCTG TAAATGCTCT TGAAGGCTTA AAAGGCGCTA ACGAAGATCA AACAGCTGGT ATCAACATTT TACGCCGTGC GATCGAAGCT CCACTTCGTC AAATCGTTGC GAATGCTGGT GATGAGCCAT CTGTAGTTAT CAACGCTGTT AAGAATGGTG AAGGTAACTT CGGTTACAAC GCTGCAACTG GCGAATATGG TGATATGTTA GAAATGGGTA TCCTTGACCC AGCTAAAGTA ACTCGTTCTG CACTTGAGCA CGCTGCTTCT GTTGCTGGCT TAATGTTAAC TACAGAATGT ATGATTACTG ACATTCCTGA AGACAAACCA GCTGCTCCAG ATATGGGCGG TATGGGTGGT ATGGGCGGAA TGATGTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT