SSGCID: Target Page

AcbaC.17932.a

glutamyl-tRNA reductase

CENTER ID:	AcbaC.17932.a
ORGANISM:	Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AcbaC.17932.a.B1.GE43943	full length		1	427

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|470.1345.peg.3038
GenBank:	AKA32800.1
PGAT:	acinetobacter:ABUW_3089
RefSeq:	WP_000007423.1
UniProt:	A0A090B5V5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSFFALGVNH QTASVELREQ IAFNAERLSN LLAEQRHHES LKDLVVVSTC NRTEVYAMAE DAESLLKWLA DANNIDVKQL IHHVYRYENA QAITHLMRVA SGLDSLMLGE PQILGQVKSA LALSKEAQTV SPELNSVFEY AFYAAKRVRS ETAVGSHAVS MGYAVAQLAL QVFSKPEKLT VMVVAAGEMN SLVAKHLAEM GVAKMIICNR SRERADQLAQ EIAHQVEVEI IDFSDLAENL YRADVVSSCT GSLHQVIAYA DVKTALKKRR YQQMLMVDLA VPRDIDPKVE SLDGVYLYGV DDLQSVIDEN LAQRRQAAVE AEVMVNQLAT QLITHQKVKE AGSTIHAYRQ HSEEISQREL THALEALHHG GNPEQVLQQF AHRLTQKLIH PTSMLLREAA KAESPDYFEW LQQHLQDVFD HERKPKR

NT Sequence

ATGTCTTTCT TTGCATTGGG TGTCAACCAT CAAACAGCTT CTGTAGAACT CCGCGAGCAA ATTGCTTTCA ATGCAGAGCG ATTAAGTAAT TTGCTTGCCG AACAACGTCA TCACGAGTCT TTAAAAGACT TAGTGGTTGT CTCAACGTGT AACCGTACAG AAGTCTACGC CATGGCGGAA GATGCCGAAA GCCTTCTGAA ATGGTTAGCT GATGCCAATA ATATTGATGT AAAGCAGTTA ATTCATCATG TTTATCGTTA CGAAAATGCT CAAGCCATCA CACATTTAAT GCGTGTAGCA AGTGGTTTGG ACTCACTCAT GTTGGGCGAA CCGCAAATTT TAGGGCAGGT GAAAAGTGCT CTAGCATTGT CTAAAGAAGC GCAAACAGTT TCTCCAGAAT TAAATAGTGT ATTTGAGTAC GCTTTTTACG CCGCTAAACG TGTGCGCTCT GAAACAGCTG TAGGTAGTCA TGCCGTTTCA ATGGGTTATG CAGTTGCCCA GCTCGCTTTA CAGGTTTTTA GTAAACCTGA AAAACTAACG GTTATGGTGG TTGCTGCTGG CGAAATGAAC AGCCTTGTTG CCAAGCATTT AGCTGAAATG GGCGTCGCCA AGATGATTAT TTGTAATCGT AGCCGTGAAC GTGCCGATCA ATTGGCCCAA GAAATTGCAC ATCAAGTTGA AGTTGAGATT ATTGATTTTT CAGATTTGGC TGAGAATCTA TATCGTGCTG ATGTTGTTTC AAGTTGTACG GGTAGTTTGC ATCAGGTTAT TGCTTATGCA GATGTCAAAA CTGCATTAAA AAAACGCCGT TATCAGCAAA TGTTAATGGT CGATTTAGCT GTACCGCGTG ATATTGACCC TAAAGTAGAG TCGCTTGATG GAGTGTATTT ATACGGCGTA GATGATCTAC AAAGTGTGAT TGATGAAAAC TTGGCTCAAC GTCGTCAAGC GGCTGTTGAA GCTGAGGTGA TGGTTAATCA GCTAGCTACC CAGCTAATTA CGCATCAAAA AGTCAAAGAG GCGGGCAGTA CAATTCATGC TTATCGCCAA CATAGTGAAG AAATTAGCCA GCGAGAGCTT ACCCATGCAT TAGAGGCTCT GCATCATGGT GGAAATCCTG AGCAAGTGTT GCAGCAATTT GCCCATCGTT TGACGCAAAA ACTAATACAT CCGACCTCTA TGTTGTTGCG TGAAGCTGCA AAAGCAGAGA GTCCGGACTA TTTTGAATGG TTACAGCAGC ATTTACAGGA TGTATTTGAT CATGAGCGAA AACCCAAACG TTAA

Details for AcbaC.17932.a.B1.GE43943

HARVESTED ON:	data unavailable
SEQUENCED ON:	12/16/2020
EXPECTED MW:	49kDa
OBSERVED MW:	49kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMS FFALGVNHQT ASVELREQIA FNAERLSNLL AEQRHHESLK DLVVVSTCNR TEVYAMAEDA ESLLKWLADA NNIDVKQLIH HVYRYENAQA ITHLMRVASG LDSLMLGEPQ ILGQVKSALA LSKEAQTVSP ELNSVFEYAF YAAKRVRSET AVGSHAVSMG YAVAQLALQV FSKPEKLTVM VVAAGEMNSL VAKHLAEMGV AKMIICNRSR ERADQLAQEI AHQVEVEIID FSDLAENLYR ADVVSSCTGS LHQVIAYADV KTALKKRRYQ QMLMVDLAVP RDIDPKVESL DGVYLYGVDD LQSVIDENLA QRRQAAVEAE VMVNQLATQL ITHQKVKEAG STIHAYRQHS EEISQRELTH ALEALHHGGN PEQVLQQFAH RLTQKLIHPT SMLLREAAKA ESPDYFEWLQ QHLQDVFD

Validated NT Sequence

atggctcacc accaccacca ccatatgtct ttctttgcat tgggtgtcaa ccatcaaaca gcttctgtag aactccgcga gcaaattgct ttcaatgcag agcgattaag taatttgctt gccgaacaac gtcatcacga gtctttaaaa gacttagtgg ttgtctcaac gtgtaaccgt acagaagtct acgccatggc ggaagatgcc gaaagccttc tgaaatggtt agctgatgcc aataatattg atgtaaagca gttaattcat catgtttatc gttacgaaaa tgctcaagcc atcacacatt taatgcgtgt agcaagtggt ttggactcac tcatgttggg cgaaccgcaa attttagggc aggtgaaaag tgctctagca ttgtctaaag aagcgcaaac agtttctcca gaattaaata gtgtatttga gtacgctttt tacgccgcta aacgtgtgcg ctctgaaaca gctgtaggta gtcatgccgt ttcaatgggt tatgcagttg cccagctcgc tttacaggtt tttagtaaac ctgaaaaact aacggttatg gtggttgctg ctggcgaaat gaacagcctt gttgccaagc atttagctga aatgggcgtc gccaagatga ttatttgtaa tcgtagccgt gaacgtgccg atcaattggc ccaagaaatt gcacatcaag ttgaagttga gattattgat ttttcagatt tggctgagaa tctatatcgt gctgatgttg tttcaagttg tacgggtagt ttgcatcagg ttattgctta tgcagatgtc aaaactgcat taaaaaaacg ccgttatcag caaatgttaa tggtcgattt agctgtaccg cgtgatattg accctaaagt agagtcgctt gatggagtgt atttatacgg cgtagatgat ctacaaagtg tgattgatga aaacttggct caacgtcgtc aagcggctgt tgaagctgag gtgatggtta atcagctagc tacccagcta attacgcatc aaaaagtcaa agaggcgggc agtacaattc atgcttatcg ccaacatagt gaagaaatta gccagcgaga gcttacccat gcattagagg ctctgcatca tggtggaaat cctgagcaag tgttgcagca atttgcccat cgtttgacgc aaaaactaat acatccgacc tctatgttgt tgcgtgaagc tgcaaaagca gagagtccgg actattttga atggttacag cagcatttac aggatgtatt tgatca

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtctt tctttgcatt gggtgtcaac catcaaacag cttctgtaga actccgcgag caaattgctt tcaatgcaga gcgattaagt aatttgcttg ccgaacaacg tcatcacgag tctttaaaag acttagtggt tgtctcaacg tgtaaccgta cagaagtcta cgccatggcg gaagatgccg aaagccttct gaaatggtta gctgatgcca ataatattga tgtaaagcag ttaattcatc atgtttatcg ttacgaaaat gctcaagcca tcacacattt aatgcgtgta gcaagtggtt tggactcact catgttgggc gaaccgcaaa ttttagggca ggtgaaaagt gctctagcat tgtctaaaga agcgcaaaca gtttctccag aattaaatag tgtatttgag tacgcttttt acgccgctaa acgtgtgcgc tctgaaacag ctgtaggtag tcatgccgtt tcaatgggtt atgcagttgc ccagctcgct ttacaggttt ttagtaaacc tgaaaaacta acggttatgg tggttgctgc tggcgaaatg aacagccttg ttgccaagca tttagctgaa atgggcgtcg ccaagatgat tatttgtaat cgtagccgtg aacgtgccga tcaattggcc caagaaattg cacatcaagt tgaagttgag attattgatt tttcagattt ggctgagaat ctatatcgtg ctgatgttgt ttcaagttgt acgggtagtt tgcatcaggt tattgcttat gcagatgtca aaactgcatt aaaaaaacgc cgttatcagc aaatgttaat ggtcgattta gctgtaccgc gtgatattga ccctaaagta gagtcgcttg atggagtgta tttatacggc gtagatgatc tacaaagtgt gattgatgaa aacttggctc aacgtcgtca agcggctgtt gaagctgagg tgatggttaa tcagctagct acccagctaa ttacgcatca aaaagtcaaa gaggcgggca gtacaattca tgcttatcgc caacatagtg aagaaattag ccagcgagag cttacccatg cattagaggc tctgcatcat ggtggaaatc ctgagcaagt gttgcagcaa tttgcccatc gtttgacgca aaaactaata catccgacct ctatgttgtt gcgtgaagct gcaaaagcag agagtccgga ctattttgaa tggttacagc agcatttaca ggatgtattt gatcatgagc gaaaacccaa acgttgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat