SSGCID: Target Page

AcbaC.17927.a

UDP-2,3-diacylglucosamine hydrolase

CENTER ID:	AcbaC.17927.a
ORGANISM:	Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AcbaC.17927.a.B1.GE38784	full length		1	239

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|470.1345.peg.1568
GenBank:	AKA31351.1
PGAT:	acinetobacter:ABUW_1613
RefSeq:	WP_000221672.1
UniProt:	A0A0D5YHV7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

VTYLFISDLH LSPEHPRLVR GFLDLLVHYQ NKQTQLYILG DWFNAWIGDD YSAPWLDEIV EALKVFNQKG NRVYFQVGNR DFALNQVFLD KFNGVLLPDI YYLNIAGHQY RLEHGDALCT DDISYQRFKK IIRNPFLVAF LRRTPLSFRT KLVNGFRKRS HSDKQQKSYE IMDVNEQAVL SALTDVDILI HGHTHRPAIH QLQHKQRVVL GDWREDHAQI LEIDPSSHLP QIKLTDWYY

NT Sequence

GTGACTTATC TGTTTATTTC AGATTTACAC TTGTCACCTG AACACCCTCG ACTCGTTCGA GGGTTTTTAG ATTTGTTGGT ACATTATCAA AATAAACAAA CACAGCTTTA TATTTTAGGT GATTGGTTTA ATGCTTGGAT TGGTGATGAC TATTCAGCGC CTTGGCTCGA CGAAATTGTC GAGGCTTTGA AAGTATTTAA CCAAAAAGGG AATCGCGTTT ATTTCCAAGT GGGTAACCGA GACTTTGCTT TAAATCAGGT TTTCCTTGAT AAGTTTAATG GTGTATTACT TCCTGATATT TATTATCTCA ACATTGCAGG TCACCAATAC CGTTTGGAAC ATGGCGATGC GTTATGTACT GATGATATTT CCTATCAAAG ATTTAAAAAA ATTATCCGAA ATCCCTTCCT AGTTGCTTTT TTACGCCGCA CCCCCTTGAG TTTCCGTACA AAACTGGTCA ATGGTTTTCG CAAAAGAAGC CATTCAGACA AACAGCAAAA AAGTTATGAA ATTATGGATG TAAATGAACA AGCTGTTCTC TCTGCCCTCA CAGATGTAGA TATACTTATT CATGGACACA CTCACCGTCC AGCAATTCAT CAATTGCAAC ATAAACAGCG GGTTGTTCTT GGTGATTGGC GTGAAGATCA TGCACAAATT TTAGAAATTG ATCCCTCTTC ACATCTGCCG CAAATCAAAC TAACTGATTG GTATTATTAA

Details for AcbaC.17927.a.B1.GE38784

HARVESTED ON:	1/22/2015
SEQUENCED ON:	1/15/2015
EXPECTED MW:	29kDa
OBSERVED MW:	29kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMV TYLFISDLHL SPEHPRLVRG FLDLLVHYQN KQTQLYILGD WFNAWIGDDY SAPWLDEIVE ALKVFNQKGN RVYFQVGNRD FALNQVFLDK FNGVLLPDIY YLNIAGHQYR LEHGDALCTD DISYQRFKKI IRNPFLVAFL RRTPLSFRTK LVNGFRKRSH SDKQQKSYEI MDVNEQAVLS ALTDVDILIH GHTHRPAIHQ LQHKQRVVLG DWREDHAQIL EIDPSSHLPQ IXXTDWYY

Validated NT Sequence

atcttactca cttaataata ccaatcagtt anntngattt gcggcagatg tgaagaggga tcaatttcta aaatttgtgc atgatcttca cgccaatcac caagaacaac ccgctgttta tgttgcaatt gatgaattgc tggacggtga gtgtgtccat gaataagtat atctacatct gtgagggcag agagaacagc ttgttcattt acatccataa tttcataact tttttgctgt ttgtctgaat ggcttctttt gcgaaaacca ttgaccagtt ttgtacggaa actcaagggg gtgcggcgta aaaaagcaac taggaaggga tttcggataa tttttttaaa tctttgatag gaaatatcat cagtacataa cgcatcgcca tgttccaaac ggtattggtg acctgcaatg ttgagataat aaatatcagg aagtaataca ccattaaact tatcaaggaa aacctgattt aaagcaaagt ctcggttacc cacttggaaa taaacgcgat tccctttttg gttaaatact ttcaaagcct cgacaatttc gtcgagccaa ggcgctgaat agtcatcacc aatccaagca ttaaaccaat cacctaaaat ataaagctgt gtttgtttat tttgataatg taccaacaaa tctaaaaacc ctcgaacgag tcgagggtgt tcaggtgaca agtgtaaatc tgaaataaac agataagtca ccatatggtg gtggtggtgg tgagccat

Expected Protein Sequence

MAHHHHHHVT YLFISDLHLS PEHPRLVRGF LDLLVHYQNK QTQLYILGDW FNAWIGDDYS APWLDEIVEA LKVFNQKGNR VYFQVGNRDF ALNQVFLDKF NGVLLPDIYY LNIAGHQYRL EHGDALCTDD ISYQRFKKII RNPFLVAFLR RTPLSFRTKL VNGFRKRSHS DKQQKSYEIM DVNEQAVLSA LTDVDILIHG HTHRPAIHQL QHKQRVVLGD WREDHAQILE IDPSSHLPQI KLTDWYY

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgactt atctgtttat ttcagattta cacttgtcac ctgaacaccc tcgactcgtt cgagggtttt tagatttgtt ggtacattat caaaataaac aaacacagct ttatatttta ggtgattggt ttaatgcttg gattggtgat gactattcag cgccttggct cgacgaaatt gtcgaggctt tgaaagtatt taaccaaaaa gggaatcgcg tttatttcca agtgggtaac cgagactttg ctttaaatca ggttttcctt gataagttta atggtgtatt acttcctgat atttattatc tcaacattgc aggtcaccaa taccgtttgg aacatggcga tgcgttatgt actgatgata tttcctatca aagatttaaa aaaattatcc gaaatccctt cctagttgct tttttacgcc gcaccccctt gagtttccgt acaaaactgg tcaatggttt tcgcaaaaga agccattcag acaaacagca aaaaagttat gaaattatgg atgtaaatga acaagctgtt ctctctgccc tcacagatgt agatatactt attcatggac acactcaccg tccagcaatt catcaattgc aacataaaca gcgggttgtt cttggtgatt ggcgtgaaga tcatgcacaa attttagaaa ttgatccctc ttcacatctg ccgcaaatca aactaactga ttggtattat tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat