AcbaC.17907.a

tRNA N6-adenosine threonylcarbamoyltransferase (EC=2.3.1.234) (N6-L-threonylcarbamoyladenine synthase) (TsaD) (YgjD)

CENTER ID: AcbaC.17907.a
ORGANISM: Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AcbaC.17907.a.B1.PW37776 full length 1 336

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|470.1345.peg.1412
GenBank: AKA31197.1
PGAT: acinetobacter:ABUW_1453
RefSeq: WP_000636263.1
UniProt: V5VBL1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIVLGLETSC DETGLALYDS ELGLRGQVLY SQIKLHAEYG GVVPELASRD HVRKLIPLMN QLLEQSGVKK QEIDAVAYTR GPGLMGALMT GALFGRTLAF SLNKPAIGVH HMEGHMLAPL LSSQPPEFPF VALLVSGGHT QLMAAHGIGQ YELLGESIDD AAGEAFDKVA KMMNLPYPGG PNIAKLALSG DPLAFEFPRP MLHQGLDFSF SGLKTAVSVQ LKKLNGENRD ADIAASFQEA IVDTLVKKSV KALKQTGLKR LVIAGGVSAN LRLREQLETS LAKIKAQVYY AEPALCTDNG AMIAFAGYQR LKAGQHDGLA VTTTPRWPMT ELTIPE
NT Sequence
ATGATTGTTT TAGGCTTGGA AACTTCTTGT GATGAAACAG GGTTGGCACT TTATGATAGC GAACTCGGCT TACGGGGACA GGTTCTTTAT AGCCAGATAA AACTTCATGC CGAATATGGT GGTGTGGTTC CAGAGTTAGC TTCTCGTGAC CATGTTCGTA AGCTTATTCC TTTAATGAAT CAGCTGCTTG AACAAAGTGG TGTTAAGAAA CAGGAAATTG ATGCAGTCGC ATATACGCGT GGCCCGGGTT TAATGGGAGC GTTAATGACC GGAGCTTTGT TTGGAAGAAC TTTAGCATTT TCTTTAAATA AACCGGCAAT TGGTGTTCAC CACATGGAAG GCCATATGTT GGCTCCTTTG CTTTCAAGCC AACCGCCTGA GTTTCCATTT GTTGCATTGC TTGTTTCAGG TGGTCATACA CAATTGATGG CAGCTCATGG TATTGGTCAA TATGAGCTAT TAGGTGAGTC AATTGATGAT GCGGCAGGTG AGGCTTTCGA CAAAGTCGCA AAAATGATGA ATCTGCCGTA TCCAGGTGGA CCAAATATTG CTAAGTTGGC GTTATCTGGT GATCCTTTAG CATTTGAATT TCCACGCCCA ATGCTTCATC AGGGCTTAGA CTTTTCTTTT AGTGGTTTAA AGACGGCAGT TTCTGTTCAG TTGAAAAAAT TGAACGGCGA AAATCGTGAT GCTGATATTG CTGCCTCATT CCAAGAAGCA ATTGTAGACA CCTTAGTAAA AAAATCTGTA AAAGCTTTAA AGCAAACTGG GCTTAAACGT TTAGTAATTG CTGGTGGGGT AAGTGCAAAT TTACGATTAC GTGAACAGTT GGAAACTTCA TTGGCGAAAA TTAAAGCTCA GGTTTACTAT GCAGAACCTG CTTTATGTAC TGATAATGGT GCAATGATTG CATTTGCAGG TTATCAGCGT TTAAAAGCAG GTCAGCATGA TGGTTTAGCT GTAACGACCA CACCAAGATG GCCAATGACT GAACTTACTA TTCCTGAGTA A
Details for AcbaC.17907.a.B1.PW37776
PURIFICATION DATe: 8/31/2015
CONCENTRATION: 11mg/ml
OBSERVED MW: 35kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 3
VIAL VOLUME: 110µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMI VLGLETSCDE TGLALYDSEL GLRGQVLYSQ IKLHAEYGGV VPELASRDHV RKLIPLMNQL LEQSGVKKQE IDAVAYTRGP GLMGALMTGA LFGRTLAFSL NKPAIGVHHM EGHMLAPLLS SQPPEFPFVA LLVSGGHTQL MAAHGIGQYE LLGESIDDAA GEAFDKVAKM MNLPYPGGPN IAKLALSGDP LAFEFPRPML HQGLDFSFSG LKTAVSVQLK KLNGENRDAD IAASFQEAIV DTLVKKSVKA LKQTGLKRLV IAGGVSANLR LREQLETSLA KIKAQVYYAE PALCTDNGAM IAFAGYQRLK AGQHDGLAVT TTPRWPMTEL TIPE
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGATTG TTTTAGGCTT GGAAACTTCT TGTGATGAAA CAGGGTTGGC ACTTTATGAT AGCGAACTCG GCTTACGGGG ACAGGTTCTT TATAGCCAGA TAAAACTTCA TGCCGAATAT GGTGGTGTGG TTCCAGAGTT AGCTTCTCGT GACCATGTTC GTAAGCTTAT TCCTTTAATG AATCAGCTGC TTGAACAAAG TGGTGTTAAG AAACAGGAAA TTGATGCAGT CGCATATACG CGTGGCCCGG GTTTAATGGG AGCGTTAATG ACCGGAGCTT TGTTTGGAAG AACTTTAGCA TTTTCTTTAA ATAAACCGGC AATTGGTGTT CACCACATGG AAGGCCATAT GTTGGCTCCT TTGCTTTCAA GCCAACCGCC TGAGTTTCCA TTTGTTGCAT TGCTTGTTTC AGGTGGTCAT ACACAATTGA TGGCAGCTCA TGGTATTGGT CAATATGAGC TATTAGGTGA GTCAATTGAT GATGCGGCAG GTGAGGCTTT CGACAAAGTC GCAAAAATGA TGAATCTGCC GTATCCAGGT GGACCAAATA TTGCTAAGTT GGCGTTATCT GGTGATCCTT TAGCATTTGA ATTTCCACGC CCAATGCTTC ATCAGGGCTT AGACTTTTCT TTTAGTGGTT TAAAGACGGC AGTTTCTGTT CAGTTGAAAA AATTGAACGG CGAAAATCGT GATGCTGATA TTGCTGCCTC ATTCCAAGAA GCAATTGTAG ACACCTTAGT AAAAAAATCT GTAAAAGCTT TAAAGCAAAC TGGGCTTAAA CGTTTAGTAA TTGCTGGTGG GGTAAGTGCA AATTTACGAT TACGTGAACA GTTGGAAACT TCATTGGCGA AAATTAAAGC TCAGGTTTAC TATGCAGAAC CTGCTTTATG TACTGATAAT GGTGCAATGA TTGCATTTGC AGGTTATCAG CGTTTAAAAG CAGGTCAGCA TGATGGTTTA GCTGTAACGA CCACACCAAG ATGGCCAATG ACTGAACTTA CTATTCCTGA GTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT