AcbaC.17905.a
thiamine-monophosphate kinase
| CENTER ID: | AcbaC.17905.a |
| ORGANISM: | Acinetobacter baumannii AB5075-UW |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
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Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| AcbaC.17905.a.B1.PW37686 | full length | 1 | 305 |
Structures
5CC8
DEPOSITED: 7/1/2015
DETERMINATION: XRay
CLONE: AcbaC.17905.a.B1.GE38781
PROTEIN: AcbaC.17905.a.B1.PW37686
5CM7
DEPOSITED: 7/16/2015
DETERMINATION: XRay
CLONE: AcbaC.17905.a.B1.GE38781
PROTEIN: AcbaC.17905.a.B1.PW37686
6MFM
DEPOSITED: 9/11/2018
DETERMINATION: XRay
CLONE: AcbaC.17905.a.B1.GE38781
PROTEIN: AcbaC.17905.a.B1.PW37686
5DD7
DEPOSITED: 8/24/2015
DETERMINATION: XRay
CLONE: AcbaC.17905.a.B1.GE38781
PROTEIN: AcbaC.17905.a.B1.PW37686
Publications by SSGCID
Crystal structures of thiamine monophosphate kinase from Acinetobacter baumannii in complex with substrates and products
Abendroth J, Dranow DM, Edwards TE, Lorimer DD, Sullivan A, Horanyi PS
Sci Rep - 2019
volume 9, pages 4392
PMID: 30867460; PMCID: PMC6416309
Towards a structome of Acinetobacter baumannii drug targets
Abendroth J, Barrett LK, Dranow DM, Edwards TE, Lorimer DD, Myler PJ, Phan I, Staker B, Subramanian S, Van Voorhis WC, Craig JC, Chun IKH, Barrett KF, Prentice RA, Tillery L, Shek P
Protein Sci. - 2020
volume 29, issue 3, pages 789-802
PMID: 31930600; PMCID: PMC7020997
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| GenBank: | AKA29887.1 |
| PATRIC ID: | fig|470.1345.peg.88 |
| PGAT: | acinetobacter:ABUW_0092 |
| RefSeq: | WP_000807403.1 |
| UniProt: | A0A0D5YC82 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAEFSIIDQY FNRQSHPDVA LGIGDDSALI TPPPNQQLVI CADTLVAGRH FPLETSPHAI GWKSVAVNLS DIAAMGAKPH SILLAISLPQ VDHEWLEGFS QGIYDCCNQF GVALIGGDTT QGPHLTITVT AMGWIETGKA VLRSGAKVGD YVCVSGQIGD AAYGLQHLGH SLQQRLDYPT PRCKLGEELK GLASSMIDVS DGLAQDLGHI LKASKVGARL ILEKLPVDPV LQQIEEQQRW QYALAGGDDY ELCFTITPQN YEKLLQKQLD VKITMIGQIV EQTKLTFEHL GSDYPLQIHG YQHFA
NT Sequence
ATGGCTGAGT TCTCAATTAT TGATCAATAC TTTAATCGTC AGTCACATCC TGACGTTGCG CTTGGTATTG GTGATGACTC AGCCTTAATT ACTCCCCCTC CAAATCAACA ACTGGTGATC TGTGCAGATA CACTTGTTGC TGGACGTCAC TTCCCTCTTG AAACCTCGCC CCATGCGATT GGCTGGAAAA GTGTTGCCGT AAATCTGTCT GATATTGCTG CAATGGGCGC CAAACCACAT AGTATTTTAC TTGCGATCAG CTTGCCTCAA GTTGACCATG AATGGCTTGA AGGATTTAGT CAGGGAATCT ATGACTGTTG TAATCAATTT GGAGTTGCCT TAATTGGCGG AGATACCACT CAAGGTCCAC ACCTCACCAT TACCGTCACG GCCATGGGCT GGATTGAAAC AGGAAAAGCT GTATTACGTT CAGGTGCTAA GGTCGGTGAT TATGTTTGTG TAAGTGGTCA GATTGGTGAT GCTGCTTATG GACTTCAGCA TTTAGGACAT TCTCTACAAC AAAGACTGGA CTATCCGACG CCACGTTGTA AGCTTGGAGA AGAGCTAAAA GGGCTCGCCT CTAGCATGAT CGATGTGTCA GATGGTTTAG CTCAAGATTT AGGACATATT TTAAAAGCGT CCAAGGTTGG AGCACGACTC ATCCTGGAAA AATTACCTGT CGATCCTGTA CTGCAACAAA TAGAAGAACA ACAACGTTGG CAATATGCCT TGGCAGGTGG TGATGACTAC GAATTATGTT TTACAATAAC ACCGCAAAAT TATGAAAAAC TTTTGCAAAA ACAACTAGAC GTTAAGATTA CAATGATCGG ACAGATCGTT GAGCAAACAA AACTAACTTT TGAGCATTTG GGTTCAGATT ACCCATTGCA AATTCATGGA TACCAACACT TTGCATAA
Details for AcbaC.17905.a.B1.PW37686
| PURIFICATION DATe: | 3/30/2015 |
| CONCENTRATION: | 50mg/ml |
| OBSERVED MW: | 34kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 16 |
| VIAL VOLUME: | 275µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMA EFSIIDQYFN RQSHPDVALG IGDDSALITP PPNQQLVICA DTLVAGRHFP LETSPHAIGW KSVAVNLSDI AAMGAKPHSI LLAISLPQVD HEWLEGFSQG IYDCCNQFGV ALIGGDTTQG PHLTITVTAM GWIETGKAVL RSGAKVGDYV CVSGQIGDAA YGLQHLGHSL QQRLDYPTPR CKLGEELKGL ASSMIDVSDG LAQDLGHILK ASKVGARLIL EKLPVDPVLQ QIEEQQRWQY ALAGGDDYEL CFTITPQNYE KLLQKQLDVK ITMIGQIVEQ TKLTFEHLGS DYPLQIHGYQ HFA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCTG AGTTCTCAAT TATTGATCAA TACTTTAATC GTCAGTCACA TCCTGACGTT GCGCTTGGTA TTGGTGATGA CTCAGCCTTA ATTACTCCCC CTCCAAATCA ACAACTGGTG ATCTGTGCAG ATACACTTGT TGCTGGACGT CACTTCCCTC TTGAAACCTC GCCCCATGCG ATTGGCTGGA AAAGTGTTGC CGTAAATCTG TCTGATATTG CTGCAATGGG CGCCAAACCA CATAGTATTT TACTTGCGAT CAGCTTGCCT CAAGTTGACC ATGAATGGCT TGAAGGATTT AGTCAGGGAA TCTATGACTG TTGTAATCAA TTTGGAGTTG CCTTAATTGG CGGAGATACC ACTCAAGGTC CACACCTCAC CATTACCGTC ACGGCCATGG GCTGGATTGA AACAGGAAAA GCTGTATTAC GTTCAGGTGC TAAGGTCGGT GATTATGTTT GTGTAAGTGG TCAGATTGGT GATGCTGCTT ATGGACTTCA GCATTTAGGA CATTCTCTAC AACAAAGACT GGACTATCCG ACGCCACGTT GTAAGCTTGG AGAAGAGCTA AAAGGGCTCG CCTCTAGCAT GATCGATGTG TCAGATGGTT TAGCTCAAGA TTTAGGACAT ATTTTAAAAG CGTCCAAGGT TGGAGCACGA CTCATCCTGG AAAAATTACC TGTCGATCCT GTACTGCAAC AAATAGAAGA ACAACAACGT TGGCAATATG CCTTGGCAGG TGGTGATGAC TACGAATTAT GTTTTACAAT AACACCGCAA AATTATGAAA AACTTTTGCA AAAACAACTA GACGTTAAGA TTACAATGAT CGGACAGATC GTTGAGCAAA CAAAACTAAC TTTTGAGCAT TTGGGTTCAG ATTACCCATT GCAAATTCAT GGATACCAAC ACTTTGCATG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT