AcbaC.17901.a

peptide chain release factor 1

CENTER ID: AcbaC.17901.a
ORGANISM: Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AcbaC.17901.a.B1.GE38776 full length 1 362
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AcbaC.17901.a.B1.PS02382 full length 1 362
AcbaC.17901.a.B1.PS38087 full length 1 362

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|470.1345.peg.1522
GenBank: AKA31305.1
PGAT: acinetobacter:ABUW_1567
RefSeq: WP_000648007.1
UniProt: A0A090B989

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKASLRLRLD QLCDRHEELT ALLADAEVIS DNKRFRKLSR EHSDLTEITE VWGKYRQAEE DIETAEMMKS DPDFKDMAEE EIQANKVLLE ELESQLNILM IPKDPNDSNA AYLEIRAGTG GDEAAIFSGD LFRMYSKYAE SQGWRIEVLS ENEGEHGGFK EVICRVDGDG VYGRLKFESG AHRVQRVPAT ESQGRVHTSA CTVAILPEID VDTNVEINPA DLRIDTYRAS GAGGQHINKT DSAVRITHIP TGTVVECQEE RSQHKNKAKA MALLVSRLEN AKRAAADAAT SEMRRDLVGS GDRSERIRTY NYPQGRMTDH RINLTLYKLD AIMEGDLTEL LDSLHREYQA DQLAMLAQEN GG
NT Sequence
ATGAAAGCGT CACTCCGTTT ACGTCTTGAT CAACTCTGTG ATCGTCATGA AGAACTTACT GCATTGCTTG CAGATGCAGA AGTGATTTCC GATAACAAAC GTTTCCGTAA ACTGTCTCGT GAACATAGTG ATTTAACTGA AATTACCGAA GTTTGGGGCA AATACCGTCA AGCAGAAGAA GATATTGAAA CTGCTGAAAT GATGAAGTCG GACCCTGACT TTAAAGATAT GGCAGAAGAA GAAATTCAAG CAAATAAAGT ATTGCTTGAA GAATTAGAAA GCCAGCTTAA TATTTTGATG ATTCCAAAAG ATCCAAATGA CTCTAATGCT GCATATTTAG AAATTCGTGC CGGGACGGGC GGTGATGAAG CTGCAATTTT CTCAGGCGAT TTATTCCGTA TGTATAGCAA ATATGCCGAA TCGCAAGGTT GGCGTATTGA AGTCCTTTCT GAAAATGAAG GCGAGCATGG TGGTTTTAAA GAAGTCATTT GCCGTGTAGA TGGTGATGGT GTGTATGGCC GCTTAAAGTT CGAAAGTGGC GCGCATCGTG TACAACGTGT TCCAGCAACT GAATCACAAG GTCGTGTGCA TACGTCAGCT TGTACAGTAG CAATCTTGCC TGAAATTGAC GTTGATACCA ATGTTGAAAT TAATCCGGCA GATTTACGTA TCGATACTTA CCGTGCATCA GGTGCGGGTG GTCAGCACAT TAACAAAACC GATTCAGCGG TGCGTATTAC TCATATTCCA ACCGGTACAG TCGTTGAGTG CCAAGAAGAG CGTTCACAGC ATAAAAACAA AGCTAAGGCG ATGGCATTGT TAGTGTCACG TTTAGAAAAT GCAAAACGCG CAGCGGCTGA TGCAGCAACT TCTGAAATGC GCCGTGATTT GGTCGGTTCT GGTGACCGCT CTGAACGTAT CCGTACTTAT AACTACCCGC AAGGACGTAT GACGGATCAC CGTATTAACC TAACTTTATA TAAGTTAGAT GCCATTATGG AAGGTGATCT AACGGAATTG CTCGATAGTT TACATCGTGA ATATCAGGCA GATCAGCTTG CGATGCTTGC ACAGGAAAAT GGCGGCTAA
Details for AcbaC.17901.a.B1.GE38776
HARVESTED ON: 1/22/2015
SEQUENCED ON: 1/15/2015
EXPECTED MW: 42kDa
OBSERVED MW: 46kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMK ASLRLRLDQL CDRHEELTAL LADAEVISDN KRFRKLSREH SDLTEITEVW GKYRQAEEDI ETAEMMKSDP DFKDMAEEEI QANKVLLEEL ESQLNILMIP KDPNDSNAAY LEIRAGTGGD EAAIFSGDLF RMYSKYAESQ GWRIEVLSEN EGEHGGFKEV ICRVDGDGVY GRLKFESGAH RVQRVPATES QGRVHTSACT VAILPEIDVD TNVEINPADL RIDTYRASGA GGQHINKTDS AVRITHIPTG TVVECQEERS QHKNKAKAMA LLVSRLENAK RAAADAATSE MRRDLVGSGD RSERIRTYNY PQGRMTDHRI NLTLYKLDAI MEGDLTELLD SLHREYQADQ LA
Validated NT Sequence
tcgcaagctg atctgcctga tattcacgat gtaaactatc gagcaattcc gttagatcac cttccataat ggcatctaac ttatataaag ttaggttaat acggtgatcc gtcatacgtc cttgcgggta gttataagta cggatacgtt cagagcggtc accagaaccg accaaatcac ggcgcatttc agaagttgct gcatcagccg ctgcgcgttt tgcattttct aaacgtgaca ctaacaatgc catcgcctta gctttgtttt tatgctgtga acgctcttct tggcactcaa cgactgtacc ggttggaata tgagtaatac gcaccgctga atcggttttg ttaatgtgct gaccacccgc acctgatgca cggtaagtat cgatacgtaa atctgccgga ttaatttcaa cattggtatc aacgtcaatt tcaggcaaga ttgctactgt acaagctgac gtatgcacac gaccttgtga ttcagttgct ggaacacgtt gtacacgatg cgcgccactt tcgaacttta agcggccata cacaccatca ccatctacac ggcaaatgac ttctttaaaa ccaccatgct cgccttcatt ttcagaaagg acttcaatac gccaaccttg cgattcggca tatttgctat acatacggaa taaatcgcct gagaaaattg cagcttcatc accgcccgtc ccggcacgaa tttctaaata tgcagcatta gagtcatttg gatcttttgg aatcatcaaa atattaagct ggctttctaa ttcttcaagc aatactttat ttgcttgaat ttcttcttct gccatatctt taaagtcagg gtccgacttc atcatttcag cagtttcaat atcttcttct gcttgacggt atttgcccca aacttcggta atttcagtta aatcactatg ttcacgagac agtttacgga aacgtttgtt atcggaaatc acttctgcat ctgcaagcaa tgcagtaagt tcttcatgac gatcacagag ttgatcaaga cgtaaacgga gtgacgcttt catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHMK ASLRLRLDQL CDRHEELTAL LADAEVISDN KRFRKLSREH SDLTEITEVW GKYRQAEEDI ETAEMMKSDP DFKDMAEEEI QANKVLLEEL ESQLNILMIP KDPNDSNAAY LEIRAGTGGD EAAIFSGDLF RMYSKYAESQ GWRIEVLSEN EGEHGGFKEV ICRVDGDGVY GRLKFESGAH RVQRVPATES QGRVHTSACT VAILPEIDVD TNVEINPADL RIDTYRASGA GGQHINKTDS AVRITHIPTG TVVECQEERS QHKNKAKAMA LLVSRLENAK RAAADAATSE MRRDLVGSGD RSERIRTYNY PQGRMTDHRI NLTLYKLDAI MEGDLTELLD SLHREYQADQ LAMLAQENGG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaag cgtcactccg tttacgtctt gatcaactct gtgatcgtca tgaagaactt actgcattgc ttgcagatgc agaagtgatt tccgataaca aacgtttccg taaactgtct cgtgaacata gtgatttaac tgaaattacc gaagtttggg gcaaataccg tcaagcagaa gaagatattg aaactgctga aatgatgaag tcggaccctg actttaaaga tatggcagaa gaagaaattc aagcaaataa agtattgctt gaagaattag aaagccagct taatattttg atgattccaa aagatccaaa tgactctaat gctgcatatt tagaaattcg tgccgggacg ggcggtgatg aagctgcaat tttctcaggc gatttattcc gtatgtatag caaatatgcc gaatcgcaag gttggcgtat tgaagtcctt tctgaaaatg aaggcgagca tggtggtttt aaagaagtca tttgccgtgt agatggtgat ggtgtgtatg gccgcttaaa gttcgaaagt ggcgcgcatc gtgtacaacg tgttccagca actgaatcac aaggtcgtgt gcatacgtca gcttgtacag tagcaatctt gcctgaaatt gacgttgata ccaatgttga aattaatccg gcagatttac gtatcgatac ttaccgtgca tcaggtgcgg gtggtcagca cattaacaaa accgattcag cggtgcgtat tactcatatt ccaaccggta cagtcgttga gtgccaagaa gagcgttcac agcataaaaa caaagctaag gcgatggcat tgttagtgtc acgtttagaa aatgcaaaac gcgcagcggc tgatgcagca acttctgaaa tgcgccgtga tttggtcggt tctggtgacc gctctgaacg tatccgtact tataactacc cgcaaggacg tatgacggat caccgtatta acctaacttt atataagtta gatgccatta tggaaggtga tctaacggaa ttgctcgata gtttacatcg tgaatatcag gcagatcagc ttgcgatgct tgcacaggaa aatggcggct gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for AcbaC.17901.a.B1.PS02382
PURIFICATION DATe: 4/16/2015
CONCENTRATION: 43mg/ml
OBSERVED MW: 49kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 2
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMK ASLRLRLDQL CDRHEELTAL LADAEVISDN KRFRKLSREH SDLTEITEVW GKYRQAEEDI ETAEMMKSDP DFKDMAEEEI QANKVLLEEL ESQLNILMIP KDPNDSNAAY LEIRAGTGGD EAAIFSGDLF RMYSKYAESQ GWRIEVLSEN EGEHGGFKEV ICRVDGDGVY GRLKFESGAH RVQRVPATES QGRVHTSACT VAILPEIDVD TNVEINPADL RIDTYRASGA GGQHINKTDS AVRITHIPTG TVVECQEERS QHKNKAKAMA LLVSRLENAK RAAADAATSE MRRDLVGSGD RSERIRTYNY PQGRMTDHRI NLTLYKLDAI MEGDLTELLD SLHREYQADQ LA
Validated NT Sequence
tcgcaagctg atctgcctga tattcacgat gtaaactatc gagcaattcc gttagatcac cttccataat ggcatctaac ttatataaag ttaggttaat acggtgatcc gtcatacgtc cttgcgggta gttataagta cggatacgtt cagagcggtc accagaaccg accaaatcac ggcgcatttc agaagttgct gcatcagccg ctgcgcgttt tgcattttct aaacgtgaca ctaacaatgc catcgcctta gctttgtttt tatgctgtga acgctcttct tggcactcaa cgactgtacc ggttggaata tgagtaatac gcaccgctga atcggttttg ttaatgtgct gaccacccgc acctgatgca cggtaagtat cgatacgtaa atctgccgga ttaatttcaa cattggtatc aacgtcaatt tcaggcaaga ttgctactgt acaagctgac gtatgcacac gaccttgtga ttcagttgct ggaacacgtt gtacacgatg cgcgccactt tcgaacttta agcggccata cacaccatca ccatctacac ggcaaatgac ttctttaaaa ccaccatgct cgccttcatt ttcagaaagg acttcaatac gccaaccttg cgattcggca tatttgctat acatacggaa taaatcgcct gagaaaattg cagcttcatc accgcccgtc ccggcacgaa tttctaaata tgcagcatta gagtcatttg gatcttttgg aatcatcaaa atattaagct ggctttctaa ttcttcaagc aatactttat ttgcttgaat ttcttcttct gccatatctt taaagtcagg gtccgacttc atcatttcag cagtttcaat atcttcttct gcttgacggt atttgcccca aacttcggta atttcagtta aatcactatg ttcacgagac agtttacgga aacgtttgtt atcggaaatc acttctgcat ctgcaagcaa tgcagtaagt tcttcatgac gatcacagag ttgatcaaga cgtaaacgga gtgacgcttt catatggtgg tggtggtggt gagccat
Expressed Protein Sequence
MAHHHHHHMK ASLRLRLDQL CDRHEELTAL LADAEVISDN KRFRKLSREH SDLTEITEVW GKYRQAEEDI ETAEMMKSDP DFKDMAEEEI QANKVLLEEL ESQLNILMIP KDPNDSNAAY LEIRAGTGGD EAAIFSGDLF RMYSKYAESQ GWRIEVLSEN EGEHGGFKEV ICRVDGDGVY GRLKFESGAH RVQRVPATES QGRVHTSACT VAILPEIDVD TNVEINPADL RIDTYRASGA GGQHINKTDS AVRITHIPTG TVVECQEERS QHKNKAKAMA LLVSRLENAK RAAADAATSE MRRDLVGSGD RSERIRTYNY PQGRMTDHRI NLTLYKLDAI MEGDLTELLD SLHREYQADQ LAMLAQENGG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAAAG CGTCACTCCG TTTACGTCTT GATCAACTCT GTGATCGTCA TGAAGAACTT ACTGCATTGC TTGCAGATGC AGAAGTGATT TCCGATAACA AACGTTTCCG TAAACTGTCT CGTGAACATA GTGATTTAAC TGAAATTACC GAAGTTTGGG GCAAATACCG TCAAGCAGAA GAAGATATTG AAACTGCTGA AATGATGAAG TCGGACCCTG ACTTTAAAGA TATGGCAGAA GAAGAAATTC AAGCAAATAA AGTATTGCTT GAAGAATTAG AAAGCCAGCT TAATATTTTG ATGATTCCAA AAGATCCAAA TGACTCTAAT GCTGCATATT TAGAAATTCG TGCCGGGACG GGCGGTGATG AAGCTGCAAT TTTCTCAGGC GATTTATTCC GTATGTATAG CAAATATGCC GAATCGCAAG GTTGGCGTAT TGAAGTCCTT TCTGAAAATG AAGGCGAGCA TGGTGGTTTT AAAGAAGTCA TTTGCCGTGT AGATGGTGAT GGTGTGTATG GCCGCTTAAA GTTCGAAAGT GGCGCGCATC GTGTACAACG TGTTCCAGCA ACTGAATCAC AAGGTCGTGT GCATACGTCA GCTTGTACAG TAGCAATCTT GCCTGAAATT GACGTTGATA CCAATGTTGA AATTAATCCG GCAGATTTAC GTATCGATAC TTACCGTGCA TCAGGTGCGG GTGGTCAGCA CATTAACAAA ACCGATTCAG CGGTGCGTAT TACTCATATT CCAACCGGTA CAGTCGTTGA GTGCCAAGAA GAGCGTTCAC AGCATAAAAA CAAAGCTAAG GCGATGGCAT TGTTAGTGTC ACGTTTAGAA AATGCAAAAC GCGCAGCGGC TGATGCAGCA ACTTCTGAAA TGCGCCGTGA TTTGGTCGGT TCTGGTGACC GCTCTGAACG TATCCGTACT TATAACTACC CGCAAGGACG TATGACGGAT CACCGTATTA ACCTAACTTT ATATAAGTTA GATGCCATTA TGGAAGGTGA TCTAACGGAA TTGCTCGATA GTTTACATCG TGAATATCAG GCAGATCAGC TTGCGATGCT TGCACAGGAA AATGGCGGCT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT
Details for AcbaC.17901.a.B1.PS38087
PURIFICATION DATe: 10/20/2016
CONCENTRATION: 11.8mg/ml
OBSERVED MW: 37kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: SEC
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 10
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMK ASLRLRLDQL CDRHEELTAL LADAEVISDN KRFRKLSREH SDLTEITEVW GKYRQAEEDI ETAEMMKSDP DFKDMAEEEI QANKVLLEEL ESQLNILMIP KDPNDSNAAY LEIRAGTGGD EAAIFSGDLF RMYSKYAESQ GWRIEVLSEN EGEHGGFKEV ICRVDGDGVY GRLKFESGAH RVQRVPATES QGRVHTSACT VAILPEIDVD TNVEINPADL RIDTYRASGA GGQHINKTDS AVRITHIPTG TVVECQEERS QHKNKAKAMA LLVSRLENAK RAAADAATSE MRRDLVGSGD RSERIRTYNY PQGRMTDHRI NLTLYKLDAI MEGDLTELLD SLHREYQADQ LA
Validated NT Sequence
tcgcaagctg atctgcctga tattcacgat gtaaactatc gagcaattcc gttagatcac cttccataat ggcatctaac ttatataaag ttaggttaat acggtgatcc gtcatacgtc cttgcgggta gttataagta cggatacgtt cagagcggtc accagaaccg accaaatcac ggcgcatttc agaagttgct gcatcagccg ctgcgcgttt tgcattttct aaacgtgaca ctaacaatgc catcgcctta gctttgtttt tatgctgtga acgctcttct tggcactcaa cgactgtacc ggttggaata tgagtaatac gcaccgctga atcggttttg ttaatgtgct gaccacccgc acctgatgca cggtaagtat cgatacgtaa atctgccgga ttaatttcaa cattggtatc aacgtcaatt tcaggcaaga ttgctactgt acaagctgac gtatgcacac gaccttgtga ttcagttgct ggaacacgtt gtacacgatg cgcgccactt tcgaacttta agcggccata cacaccatca ccatctacac ggcaaatgac ttctttaaaa ccaccatgct cgccttcatt ttcagaaagg acttcaatac gccaaccttg cgattcggca tatttgctat acatacggaa taaatcgcct gagaaaattg cagcttcatc accgcccgtc ccggcacgaa tttctaaata tgcagcatta gagtcatttg gatcttttgg aatcatcaaa atattaagct ggctttctaa ttcttcaagc aatactttat ttgcttgaat ttcttcttct gccatatctt taaagtcagg gtccgacttc atcatttcag cagtttcaat atcttcttct gcttgacggt atttgcccca aacttcggta atttcagtta aatcactatg ttcacgagac agtttacgga aacgtttgtt atcggaaatc acttctgcat ctgcaagcaa tgcagtaagt tcttcatgac gatcacagag ttgatcaaga cgtaaacgga gtgacgcttt catatggtgg tggtggtggt gagccat
Expressed Protein Sequence
MAHHHHHHMK ASLRLRLDQL CDRHEELTAL LADAEVISDN KRFRKLSREH SDLTEITEVW GKYRQAEEDI ETAEMMKSDP DFKDMAEEEI QANKVLLEEL ESQLNILMIP KDPNDSNAAY LEIRAGTGGD EAAIFSGDLF RMYSKYAESQ GWRIEVLSEN EGEHGGFKEV ICRVDGDGVY GRLKFESGAH RVQRVPATES QGRVHTSACT VAILPEIDVD TNVEINPADL RIDTYRASGA GGQHINKTDS AVRITHIPTG TVVECQEERS QHKNKAKAMA LLVSRLENAK RAAADAATSE MRRDLVGSGD RSERIRTYNY PQGRMTDHRI NLTLYKLDAI MEGDLTELLD SLHREYQADQ LAMLAQENGG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAAAG CGTCACTCCG TTTACGTCTT GATCAACTCT GTGATCGTCA TGAAGAACTT ACTGCATTGC TTGCAGATGC AGAAGTGATT TCCGATAACA AACGTTTCCG TAAACTGTCT CGTGAACATA GTGATTTAAC TGAAATTACC GAAGTTTGGG GCAAATACCG TCAAGCAGAA GAAGATATTG AAACTGCTGA AATGATGAAG TCGGACCCTG ACTTTAAAGA TATGGCAGAA GAAGAAATTC AAGCAAATAA AGTATTGCTT GAAGAATTAG AAAGCCAGCT TAATATTTTG ATGATTCCAA AAGATCCAAA TGACTCTAAT GCTGCATATT TAGAAATTCG TGCCGGGACG GGCGGTGATG AAGCTGCAAT TTTCTCAGGC GATTTATTCC GTATGTATAG CAAATATGCC GAATCGCAAG GTTGGCGTAT TGAAGTCCTT TCTGAAAATG AAGGCGAGCA TGGTGGTTTT AAAGAAGTCA TTTGCCGTGT AGATGGTGAT GGTGTGTATG GCCGCTTAAA GTTCGAAAGT GGCGCGCATC GTGTACAACG TGTTCCAGCA ACTGAATCAC AAGGTCGTGT GCATACGTCA GCTTGTACAG TAGCAATCTT GCCTGAAATT GACGTTGATA CCAATGTTGA AATTAATCCG GCAGATTTAC GTATCGATAC TTACCGTGCA TCAGGTGCGG GTGGTCAGCA CATTAACAAA ACCGATTCAG CGGTGCGTAT TACTCATATT CCAACCGGTA CAGTCGTTGA GTGCCAAGAA GAGCGTTCAC AGCATAAAAA CAAAGCTAAG GCGATGGCAT TGTTAGTGTC ACGTTTAGAA AATGCAAAAC GCGCAGCGGC TGATGCAGCA ACTTCTGAAA TGCGCCGTGA TTTGGTCGGT TCTGGTGACC GCTCTGAACG TATCCGTACT TATAACTACC CGCAAGGACG TATGACGGAT CACCGTATTA ACCTAACTTT ATATAAGTTA GATGCCATTA TGGAAGGTGA TCTAACGGAA TTGCTCGATA GTTTACATCG TGAATATCAG GCAGATCAGC TTGCGATGCT TGCACAGGAA AATGGCGGCT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT