SSGCID: Target Page

AcbaC.17895.a

tRNA nucleotidyl transferase

CENTER ID:	AcbaC.17895.a
ORGANISM:	Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AcbaC.17895.a.B1.GE38773	full length		1	412

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AcbaC.17895.a.B1.PS38106	full length		1	412

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|470.1345.peg.1180
GenBank:	AKA30967.1
PGAT:	acinetobacter:ABUW_1219
RefSeq:	WP_001198539.1
UniProt:	A0A059ZQA3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MQVYLVGGAV RDYLLGHPYQ EKDYVVVGAT PEHMLAQGFQ PVGKDFPVFL HPETKEEYAL ARTERKSGQG YHGFQFFTDT TVSLEDDLIR RDLTINAIAM DQDGKIYDPY GGQNDLENKI LRHVSEAFAE DPLRVLRVAR FAARYFPYGF QIAPETLQLM QTMADSGELD ALTPERVWKE TSRALMENHA DIYFQTLRDC GALKHLFPEI DALFGVPQRP EYHPEVDCGI HTLMSLQQAC KSNYSLDVRF AVLVHDLGKA LTPAEELPRH IMHEERGIKP VTQLCERLRV PTQTKQLALS VCKEHLKCHQ IMSLKPGTLW RLLQRLDVLR RPERVEAFVQ ACECDAKGRL GLEDRPYPQA QYMREAMQIV RSIKVQDLPE NIKGAEIGEM LIQYRIEALA EFKNQHQSLS HS

NT Sequence

ATGCAAGTGT ATTTAGTAGG CGGTGCTGTT CGAGATTATT TACTTGGGCA TCCCTATCAA GAAAAAGACT ATGTTGTGGT CGGTGCAACG CCGGAACACA TGCTCGCCCA AGGTTTTCAA CCTGTAGGCA AAGATTTCCC TGTGTTTCTT CATCCCGAAA CCAAGGAAGA ATACGCACTT GCACGAACCG AACGAAAGTC TGGACAGGGA TATCACGGGT TTCAATTCTT TACTGATACA ACCGTTAGTT TAGAAGATGA CTTAATTCGT CGAGACTTAA CCATTAATGC GATTGCAATG GATCAAGATG GTAAAATATA TGATCCATAT GGTGGCCAAA ACGACTTAGA AAATAAAATT CTGCGCCACG TTTCAGAAGC TTTTGCTGAA GACCCTTTAC GGGTATTGCG AGTCGCGCGT TTTGCCGCCC GATATTTCCC ATATGGTTTT CAAATTGCGC CAGAGACATT GCAACTTATG CAAACCATGG CAGATTCAGG TGAACTTGAT GCACTAACAC CAGAGCGCGT CTGGAAAGAA ACTTCACGCG CTTTAATGGA AAATCATGCC GATATTTATT TCCAAACTTT GCGTGATTGT GGGGCTTTAA AACATCTTTT CCCTGAGATT GATGCTTTAT TCGGTGTGCC TCAACGTCCC GAATATCATC CTGAAGTGGA TTGCGGCATT CATACTTTAA TGTCATTACA GCAAGCTTGT AAGTCGAACT ACTCGCTTGA TGTACGTTTC GCTGTTTTAG TGCATGATCT TGGTAAGGCC CTCACTCCAG CCGAAGAACT TCCTCGCCAT ATCATGCATG AAGAACGCGG GATTAAGCCA GTTACCCAAT TATGTGAACG TTTAAGAGTT CCGACACAGA CCAAACAATT AGCCTTATCT GTTTGTAAAG AACATTTAAA ATGTCATCAG ATTATGTCCT TGAAACCTGG TACTTTATGG CGTCTATTGC AACGCTTAGA TGTTTTACGT CGCCCAGAAA GAGTGGAAGC TTTTGTACAA GCCTGCGAAT GTGATGCAAA AGGCAGATTA GGACTAGAAG ATCGTCCATA TCCTCAAGCT CAATACATGC GTGAAGCCAT GCAAATTGTT CGCTCCATTA AAGTTCAAGA TTTACCAGAG AACATTAAGG GTGCTGAAAT TGGTGAAATG CTTATTCAAT ATCGAATTGA AGCTTTAGCT GAATTTAAAA ATCAGCATCA AAGTCTTAGT CACTCCTAA

Details for AcbaC.17895.a.B1.GE38773

HARVESTED ON:	1/22/2015
SEQUENCED ON:	1/15/2015
EXPECTED MW:	48kDa
OBSERVED MW:	43kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMX XYLVGGAVRD YLLGHPYQEK DYVVVGATPE HMLAQGFQPV GKDFPVFLHP ETKEEYALAR TERKSGQGYH GFQFFTDTTV SLEDDLIRRD LTINAIAMDQ DGKIYDPYGG QNDLENKILR HVSEAFAEDP LRVLRVARFA ARYFPYGFQI APETLQLMQT MADSGELDAL TPERVWKETS RALMENHADI YFQTLRDCGA LKHLFPEIDA LFGVPQRPEY HPEVDCGIHT LMSLQQACKS NYSLDVRFAV LVHDLGKALT PAEELPRHIM HEERGIKPVT QLCERLRVPT QTKQLALSVC KEHLKCHQIM SLKPGTLWRL LQRLDVLRRP ERVEAFVQAC ECDAKGRLGL EDRPYPQAQY MREAMQIVRS IKVQDLPENI KGAEIGEMLI QYRIEALAEF KN

Validated NT Sequence

gatttttaaa ttcagctaaa gcttcaattc gatattgaat aagcatttca ccaatttcag cacccttaat gttctctggt aaatcttgaa ctttaatgga gcgaacaatt tgcatggctt cacgcatgta ttgagcttga ggatatggac gatcttctag tcctaatctg ccttttgcat cacattcgca ggcttgtaca aaagcttcca ctctttctgg gcgacgtaaa acatctaagc gttgcaatag acgccataaa gtaccaggtt tcaaggacat aatctgatga cattttaaat gttctttaca aacagataag gctaattgtt tggtctgtgt cggaactctt aaacgttcac ataattgggt aactggctta atcccgcgtt cttcatgcat gatatggcga ggaagttctt cggctggagt gagggcctta ccaagatcat gcactaaaac agcgaaacgt acatcaagcg agtagttcga cttacaagct tgctgtaatg acattaaagt atgaatgccg caatccactt caggatgata ttcgggacgt tgaggcacac cgaataaagc atcaatctca gggaaaagat gttttaaagc cccacaatca cgcaaagttt ggaaataaat atcggcatga ttttccatta aagcgcgtga agtttctttc cagacgcgct ctggtgttag tgcatcaagt tcacctgaat ctgccatggt ttgcataagt tgcaatgtct ctggcgcaat ttgaaaacca tatgggaaat atcgggcggc aaaacgcgcg actcgcaata cccgtaaagg gtcttcagca aaagcttctg aaacgtggcg cagaatttta ttttctaagt cgttttggcc accatatgga tcatatattt taccatcttg atccattgca atcgcattaa tggttaagtc tcgacgaatt aagtcatctt ctaaactaac ggttgtatca gtaaagaatt gaaacccgtg atatccctgt ccagactttc gttcggttcg tgcaagtgcg tattcttcct tggtttcggg atgaagaaac acagggaaat ctttgcctac aggttgaaaa ccttgggcga gcatgtgttc cggcgttgca ccgaccacaa catagtcttt ttcttgatag ggatgcccaa gtaaataatc tcgaacagca ccgcctacta aatacnnnnn catatggtgg tggtggtggt gagccat

Expected Protein Sequence

MAHHHHHHMQ VYLVGGAVRD YLLGHPYQEK DYVVVGATPE HMLAQGFQPV GKDFPVFLHP ETKEEYALAR TERKSGQGYH GFQFFTDTTV SLEDDLIRRD LTINAIAMDQ DGKIYDPYGG QNDLENKILR HVSEAFAEDP LRVLRVARFA ARYFPYGFQI APETLQLMQT MADSGELDAL TPERVWKETS RALMENHADI YFQTLRDCGA LKHLFPEIDA LFGVPQRPEY HPEVDCGIHT LMSLQQACKS NYSLDVRFAV LVHDLGKALT PAEELPRHIM HEERGIKPVT QLCERLRVPT QTKQLALSVC KEHLKCHQIM SLKPGTLWRL LQRLDVLRRP ERVEAFVQAC ECDAKGRLGL EDRPYPQAQY MREAMQIVRS IKVQDLPENI KGAEIGEMLI QYRIEALAEF KNQHQSLSHS

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcaag tgtatttagt aggcggtgct gttcgagatt atttacttgg gcatccctat caagaaaaag actatgttgt ggtcggtgca acgccggaac acatgctcgc ccaaggtttt caacctgtag gcaaagattt ccctgtgttt cttcatcccg aaaccaagga agaatacgca cttgcacgaa ccgaacgaaa gtctggacag ggatatcacg ggtttcaatt ctttactgat acaaccgtta gtttagaaga tgacttaatt cgtcgagact taaccattaa tgcgattgca atggatcaag atggtaaaat atatgatcca tatggtggcc aaaacgactt agaaaataaa attctgcgcc acgtttcaga agcttttgct gaagaccctt tacgggtatt gcgagtcgcg cgttttgccg cccgatattt cccatatggt tttcaaattg cgccagagac attgcaactt atgcaaacca tggcagattc aggtgaactt gatgcactaa caccagagcg cgtctggaaa gaaacttcac gcgctttaat ggaaaatcat gccgatattt atttccaaac tttgcgtgat tgtggggctt taaaacatct tttccctgag attgatgctt tattcggtgt gcctcaacgt cccgaatatc atcctgaagt ggattgcggc attcatactt taatgtcatt acagcaagct tgtaagtcga actactcgct tgatgtacgt ttcgctgttt tagtgcatga tcttggtaag gccctcactc cagccgaaga acttcctcgc catatcatgc atgaagaacg cgggattaag ccagttaccc aattatgtga acgtttaaga gttccgacac agaccaaaca attagcctta tctgtttgta aagaacattt aaaatgtcat cagattatgt ccttgaaacc tggtacttta tggcgtctat tgcaacgctt agatgtttta cgtcgcccag aaagagtgga agcttttgta caagcctgcg aatgtgatgc aaaaggcaga ttaggactag aagatcgtcc atatcctcaa gctcaataca tgcgtgaagc catgcaaatt gttcgctcca ttaaagttca agatttacca gagaacatta agggtgctga aattggtgaa atgcttattc aatatcgaat tgaagcttta gctgaattta aaaatcagca tcaaagtctt agtcactcct gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat

Details for AcbaC.17895.a.B1.PS38106

PURIFICATION DATe:	11/9/2016
CONCENTRATION:	31.7mg/ml
OBSERVED MW:	45kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	SEC Buffer
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	1
VIAL VOLUME:	110µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	98

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCAAG TGTATTTAGT AGGCGGTGCT GTTCGAGATT ATTTACTTGG GCATCCCTAT CAAGAAAAAG ACTATGTTGT GGTCGGTGCA ACGCCGGAAC ACATGCTCGC CCAAGGTTTT CAACCTGTAG GCAAAGATTT CCCTGTGTTT CTTCATCCCG AAACCAAGGA AGAATACGCA CTTGCACGAA CCGAACGAAA GTCTGGACAG GGATATCACG GGTTTCAATT CTTTACTGAT ACAACCGTTA GTTTAGAAGA TGACTTAATT CGTCGAGACT TAACCATTAA TGCGATTGCA ATGGATCAAG ATGGTAAAAT ATATGATCCA TATGGTGGCC AAAACGACTT AGAAAATAAA ATTCTGCGCC ACGTTTCAGA AGCTTTTGCT GAAGACCCTT TACGGGTATT GCGAGTCGCG CGTTTTGCCG CCCGATATTT CCCATATGGT TTTCAAATTG CGCCAGAGAC ATTGCAACTT ATGCAAACCA TGGCAGATTC AGGTGAACTT GATGCACTAA CACCAGAGCG CGTCTGGAAA GAAACTTCAC GCGCTTTAAT GGAAAATCAT GCCGATATTT ATTTCCAAAC TTTGCGTGAT TGTGGGGCTT TAAAACATCT TTTCCCTGAG ATTGATGCTT TATTCGGTGT GCCTCAACGT CCCGAATATC ATCCTGAAGT GGATTGCGGC ATTCATACTT TAATGTCATT ACAGCAAGCT TGTAAGTCGA ACTACTCGCT TGATGTACGT TTCGCTGTTT TAGTGCATGA TCTTGGTAAG GCCCTCACTC CAGCCGAAGA ACTTCCTCGC CATATCATGC ATGAAGAACG CGGGATTAAG CCAGTTACCC AATTATGTGA ACGTTTAAGA GTTCCGACAC AGACCAAACA ATTAGCCTTA TCTGTTTGTA AAGAACATTT AAAATGTCAT CAGATTATGT CCTTGAAACC TGGTACTTTA TGGCGTCTAT TGCAACGCTT AGATGTTTTA CGTCGCCCAG AAAGAGTGGA AGCTTTTGTA CAAGCCTGCG AATGTGATGC AAAAGGCAGA TTAGGACTAG AAGATCGTCC ATATCCTCAA GCTCAATACA TGCGTGAAGC CATGCAAATT GTTCGCTCCA TTAAAGTTCA AGATTTACCA GAGAACATTA AGGGTGCTGA AATTGGTGAA ATGCTTATTC AATATCGAAT TGAAGCTTTA GCTGAATTTA AAAATCAGCA TCAAAGTCTT AGTCACTCCT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT