AcbaC.17885.a
aspartate-semialdehyde dehydrogenase
| CENTER ID: | AcbaC.17885.a |
| ORGANISM: | Acinetobacter baumannii AB5075-UW |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| AcbaC.17885.a.B1.GE43941 | full length | 1 | 372 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
7SKB
DEPOSITED: 10/20/2021
DETERMINATION: XRay
CLONE: AcbaC.17885.a.B1.GE43941
PROTEIN: AcbaC.17885.a.B1.PW38929
7TCM
DEPOSITED: 12/27/2021
DETERMINATION: XRay
CLONE: AcbaC.17885.a.B1.GE43941
PROTEIN: AcbaC.17885.a.B1.PW38929
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| GenBank: | AKA33147.1 |
| PATRIC ID: | fig|470.1345.peg.3387 |
| PGAT: | acinetobacter:ABUW_3451 |
| RefSeq: | WP_000862762.1 |
| UniProt: | V5VGT0 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKVGLVGWRG MVGSVLMQRM VEENDFAHIE PFYFSTSNAG GEAPSFGGKT APALMEATDI TSLKQMDVII TCQGGDYTSE VFPKLKATGW DGYWIDAAST LRMTDDAIIV LDPVNLNVIK DGLAKGTKTF VGGNCTVSLM LMGVGALFQN NLVEWMTAMT YQAASGAGAQ NMRELLTGMG YLYNNTKTLL DDPKSAILDI DRQVAELQRG EGFPSANFGV PLAGSLIPYI DKQLESGQSK EEWKGQVETN KILGNSQIVP IDGHCVRIGA MRCHSQALTI KLKKDVPLDE IEDMIRNSNQ WAKVVPNTRE ASMTDLTPVA VTGTLTVPVG RLRKLNMGKE YLGAFTVGDQ LLWGAAEPLR RMLRILVEYK SS
NT Sequence
ATGAAAGTAG GTCTGGTCGG TTGGCGCGGG ATGGTCGGTT CCGTCCTTAT GCAACGTATG GTTGAAGAGA ATGATTTTGC TCATATTGAG CCATTCTACT TCTCTACCAG TAATGCAGGT GGTGAAGCTC CTTCATTTGG TGGTAAGACT GCCCCAGCAC TTATGGAAGC TACAGACATT ACCAGTCTGA AGCAAATGGA TGTCATTATT ACCTGTCAAG GTGGTGACTA CACGTCTGAA GTTTTCCCAA AGTTAAAAGC AACTGGTTGG GATGGCTACT GGATTGATGC GGCATCTACT TTACGTATGA CTGATGATGC AATCATCGTT CTTGATCCGG TTAACCTTAA TGTAATTAAA GACGGTTTAG CGAAAGGTAC TAAAACTTTC GTGGGTGGTA ACTGTACAGT ATCACTCATG TTGATGGGTG TTGGCGCACT TTTCCAAAAT AATTTGGTTG AGTGGATGAC TGCAATGACT TATCAAGCGG CATCAGGCGC TGGCGCACAA AACATGCGTG AGTTGTTAAC AGGTATGGGT TATTTATATA ACAATACCAA GACTTTGTTA GATGATCCTA AATCTGCAAT TCTTGATATT GACCGTCAAG TTGCCGAGTT ACAACGCGGT GAAGGTTTCC CTTCTGCGAA CTTTGGTGTG CCACTTGCTG GTTCATTAAT TCCTTACATT GATAAACAGC TTGAAAGCGG TCAGTCAAAA GAAGAGTGGA AAGGACAAGT TGAAACCAAC AAGATCTTAG GTAACTCTCA AATCGTTCCT ATCGATGGTC ACTGTGTTCG TATCGGTGCA ATGCGTTGCC ACTCTCAAGC ATTAACCATC AAATTGAAAA AAGATGTTCC GCTTGATGAG ATCGAAGATA TGATTCGTAA CTCAAACCAG TGGGCTAAAG TTGTACCAAA TACACGTGAA GCATCAATGA CTGATCTTAC ACCTGTTGCT GTAACTGGTA CCCTAACTGT ACCTGTAGGT CGTTTACGTA AACTCAATAT GGGTAAAGAG TACCTCGGCG CATTCACAGT AGGCGATCAG TTACTTTGGG GTGCTGCTGA GCCTCTACGT CGTATGTTAC GTATTTTAGT AGAATACAAA AGCTCATAA
Details for AcbaC.17885.a.B1.GE43941
| HARVESTED ON: | 12/3/2020 |
| SEQUENCED ON: | 12/16/2020 |
| EXPECTED MW: | 42kDa |
| OBSERVED MW: | 42kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MAHHHHHHMK VGLVGWRGMV GSVLMQRMVE ENDFAHIEPF YFSTSNAGGE APSFGGKTAP ALMEATDITS LKQMDVIITC QGGDYTSEVF PKLKATGWDG YWIDAASTLR MTDDAIIVLD PVNLNVIKDG LAKGTKTFVG GNCTVSLMLM GVGALFQNNL VEWMTAMTYQ AASGAGAQNM RELLTGMGYL YNNTKTLLDD PKSAILDIDR QVAELQRGEG FPSANFGVPL AGSLIPYIDK QLESGQSKEE WKGQVETNKI LGNSQIVPID GHCVRIGAMR CHSQALTIKL KKDVPLDEIE DMIRNSNQWA KVVPNTREAS MTDLTPVAVT GTLTVPVGRL RKLNMGKEYL GAFTVGDQLL WGAAEPLRRM LR
Validated NT Sequence
atggctcacc accaccacca ccatatgaaa gtaggtctgg tcggttggcg cgggatggtc ggttccgtcc ttatgcaacg tatggttgaa gagaatgatt ttgctcatat tgagccattc tacttctcta ccagtaatgc aggtggtgaa gctccttcat ttggtggtaa gactgcccca gcacttatgg aagctacaga cattaccagt ctgaagcaaa tggatgtcat tattacctgt caaggtggtg actacacgtc tgaagttttc ccaaagttaa aagcaactgg ttgggatggc tactggattg atgcggcatc tactttacgt atgactgatg atgcaatcat cgttcttgat ccggttaacc ttaatgtaat taaagacggt ttagcgaaag gtactaaaac tttcgtgggt ggtaactgta cagtatcact catgttgatg ggtgttggcg cacttttcca aaataatttg gttgagtgga tgactgcaat gacttatcaa gcggcatcag gcgctggcgc acaaaacatg cgtgagttgt taacaggtat gggttattta tataacaata ccaagacttt gttagatgat cctaaatctg caattcttga tattgaccgt caagttgccg agttacaacg cggtgaaggt ttcccttctg cgaactttgg tgtgccactt gctggttcat taattcctta cattgataaa cagcttgaaa gcggtcagtc aaaagaagag tggaaaggac aagttgaaac caacaagatc ttaggtaact ctcaaatcgt tcctatcgat ggtcactgtg ttcgtatcgg tgcaatgcgt tgccactctc aagcattaac catcaaattg aaaaaagatg ttccgcttga tgagatcgaa gatatgattc gtaactcaaa ccagtgggct aaagttgtac caaatacacg tgaagcatca atgactgatc ttacacctgt tgctgtaact ggtaccctaa ctgtacctgt aggtcgttta cgtaaactca atatgggtaa agagtacctc ggcgcattca cagtaggcga tcagttactt tggggtgctg ctgagcctct acgtcgtatg ttacgtat
Expected Protein Sequence
MAHHHHHHMK VGLVGWRGMV GSVLMQRMVE ENDFAHIEPF YFSTSNAGGE APSFGGKTAP ALMEATDITS LKQMDVIITC QGGDYTSEVF PKLKATGWDG YWIDAASTLR MTDDAIIVLD PVNLNVIKDG LAKGTKTFVG GNCTVSLMLM GVGALFQNNL VEWMTAMTYQ AASGAGAQNM RELLTGMGYL YNNTKTLLDD PKSAILDIDR QVAELQRGEG FPSANFGVPL AGSLIPYIDK QLESGQSKEE WKGQVETNKI LGNSQIVPID GHCVRIGAMR CHSQALTIKL KKDVPLDEIE DMIRNSNQWA KVVPNTREAS MTDLTPVAVT GTLTVPVGRL RKLNMGKEYL GAFTVGDQLL WGAAEPLRRM LRILVEYKSS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaag taggtctggt cggttggcgc gggatggtcg gttccgtcct tatgcaacgt atggttgaag agaatgattt tgctcatatt gagccattct acttctctac cagtaatgca ggtggtgaag ctccttcatt tggtggtaag actgccccag cacttatgga agctacagac attaccagtc tgaagcaaat ggatgtcatt attacctgtc aaggtggtga ctacacgtct gaagttttcc caaagttaaa agcaactggt tgggatggct actggattga tgcggcatct actttacgta tgactgatga tgcaatcatc gttcttgatc cggttaacct taatgtaatt aaagacggtt tagcgaaagg tactaaaact ttcgtgggtg gtaactgtac agtatcactc atgttgatgg gtgttggcgc acttttccaa aataatttgg ttgagtggat gactgcaatg acttatcaag cggcatcagg cgctggcgca caaaacatgc gtgagttgtt aacaggtatg ggttatttat ataacaatac caagactttg ttagatgatc ctaaatctgc aattcttgat attgaccgtc aagttgccga gttacaacgc ggtgaaggtt tcccttctgc gaactttggt gtgccacttg ctggttcatt aattccttac attgataaac agcttgaaag cggtcagtca aaagaagagt ggaaaggaca agttgaaacc aacaagatct taggtaactc tcaaatcgtt cctatcgatg gtcactgtgt tcgtatcggt gcaatgcgtt gccactctca agcattaacc atcaaattga aaaaagatgt tccgcttgat gagatcgaag atatgattcg taactcaaac cagtgggcta aagttgtacc aaatacacgt gaagcatcaa tgactgatct tacacctgtt gctgtaactg gtaccctaac tgtacctgta ggtcgtttac gtaaactcaa tatgggtaaa gagtacctcg gcgcattcac agtaggcgat cagttacttt ggggtgctgc tgagcctcta cgtcgtatgt tacgtatttt agtagaatac aaaagctcat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat