AcbaC.17879.a

tRNA(Ile)-lysidine synthase

CENTER ID: AcbaC.17879.a
ORGANISM: Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AcbaC.17879.a.B2.GE38771 mature protein 18 424
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|470.1345.peg.3207
GenBank: AKA32969.1
PGAT: acinetobacter:ABUW_3273
UniProt: A0A0D5YLZ8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLLLHLMAQI FPQKIRAIYI DHQLQDQSAE WAEVVATQAT ILNIPYIIQK VQIANGNLEA QARQARYQAY QQHLQENEIL LLAHHQQDQA ETVILRLLSG AGVDGLAAMQ AIDYRKDMTI WRPFLDLTRE QIALWTAQLE VKYIDDPMNY DNHYDRVWCR EALWPFLTSR FPKMQQALSR TSYLMQDASE ILEEVLKDDW QYSGSADYLD LTKLSELSFA RQRQLLSAWM KGQGQYRPAF EMVERLRAEV IESKSDAQAA LHWNQFYYVR YQNILYRLSK QIYFAETLNP VAAELEHSFK LEEKWQGAAG LFHVEYKKIG LSHSLLNKKL KIIRRQGGEK IHLYGRVGQW PLKKAIQEAH ILPWLRHTIQ ILVLDNVMLG VFTPKGFWLA QSPYCEEGGW QPDLISHSCN LVNGEYSYGN SSEL
NT Sequence
ATGCTACTGC TACATTTAAT GGCTCAAATT TTCCCTCAAA AGATCCGAGC AATTTATATT GATCATCAAC TGCAAGATCA AAGTGCAGAG TGGGCGGAAG TGGTTGCCAC ACAGGCAACA ATATTAAATA TTCCTTATAT TATTCAAAAA GTTCAGATCG CAAATGGCAA TCTGGAAGCA CAAGCACGTC AAGCGCGTTA TCAAGCATAT CAGCAACATT TACAAGAAAA TGAAATTCTG TTGCTTGCTC ATCATCAACA AGATCAGGCA GAAACAGTTA TTTTGCGATT ATTGTCAGGT GCGGGAGTAG ATGGACTTGC TGCGATGCAG GCTATTGATT ACCGTAAAGA TATGACAATT TGGCGCCCCT TTTTGGATTT AACACGTGAG CAAATCGCCT TATGGACTGC TCAGCTTGAA GTTAAATATA TTGACGATCC AATGAACTAT GACAACCATT ACGATAGGGT GTGGTGTCGT GAAGCACTGT GGCCATTTTT AACAAGCCGT TTTCCAAAAA TGCAGCAAGC TTTGAGTCGA ACCAGTTATC TGATGCAAGA TGCTTCTGAA ATTTTAGAAG AAGTTCTTAA AGATGATTGG CAATATTCTG GTTCAGCTGA CTATTTAGAT TTAACGAAAT TATCCGAGTT ATCTTTTGCG CGGCAGCGCC AGTTACTTTC TGCGTGGATG AAAGGCCAAG GGCAATATCG ACCTGCATTT GAAATGGTCG AACGGCTAAG AGCAGAAGTT ATTGAGTCTA AGTCAGATGC TCAAGCCGCT TTACATTGGA ACCAGTTTTA CTATGTCCGT TATCAAAATA TACTCTATAG GTTGAGTAAA CAGATTTATT TCGCTGAAAC TTTAAATCCG GTTGCTGCTG AACTAGAGCA TTCATTTAAA TTAGAAGAAA AATGGCAGGG CGCAGCTGGA CTGTTCCATG TTGAGTATAA AAAAATTGGT CTAAGTCATT CTTTATTGAA TAAAAAATTA AAAATCATTC GACGTCAAGG TGGGGAAAAG ATTCACCTAT ATGGTCGTGT TGGGCAATGG CCTTTAAAAA AAGCAATTCA AGAAGCACAT ATTTTGCCTT GGCTTCGTCA TACAATTCAA ATATTAGTCT TAGATAATGT TATGCTTGGG GTTTTTACAC CGAAAGGATT TTGGTTGGCT CAGTCTCCAT ATTGTGAGGA AGGAGGGTGG CAACCAGATT TAATTTCTCA TTCTTGTAAT CTGGTAAATG GCGAATATAG TTATGGCAAC AGCAGTGAAT TGTAA
Details for AcbaC.17879.a.B2.GE38771
HARVESTED ON: 1/22/2015
SEQUENCED ON: 1/15/2015
EXPECTED MW: 48kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHXX XIDHQLQDQS AEWAEVVATQ ATILNIPYII QKVQIANGNL EAQARQARYQ AYQQHLQENE ILLLAHHQQD QAETVILRLL SGAGVDGLAA MQAIDYRKDM TIWRPFLDLT REQIALWTAQ LEVKYIDDPM NYDNHYDRVW CREALWPFLT SRFPKMQQAL SRTSYLMQDA SEILEEVLKD DWQYSGSADY LDLTKLSELS FARQRQLLSA WMKGQGQYRP AFEMVERLRA EVIESKSDAQ AALHWNQFYY VRYQNILYRL SKQIYFAETL NPVAAELEHS FKLEEKWQGA AGLFHVEYKK IGLSHSLLNK KLKIIRRQGG EKIHLYGRVG QWPLKKAIQE AHILPWLRHT IQILVLDNVM LGVFTPKGFW LAQSPYCEEG GWQPDLISHS CNLVNGE
Validated NT Sequence
tattcgccat ttaccagatt acaagaatga gaaattaaat ctggttgcca ccctccttcc tcacaatatg gagactgagc caaccaaaat cctttcggtg taaaaacccc aagcataaca ttatctaaga ctaatatttg aattgtatga cgaagccaag gcaaaatatg tgcttcttga attgcttttt ttaaaggcca ttgcccaaca cgaccatata ggtgaatctt ttccccacct tgacgtcgaa tgatttttaa ttttttattc aataaagaat gacttagacc aattttttta tactcaacat ggaacagtcc agctgcgccc tgccattttt cttctaattt aaatgaatgc tctagttcag cagcaaccgg atttaaagtt tcagcgaaat aaatctgttt actcaaccta tagagtatat tttgataacg gacatagtaa aactggttcc aatgtaaagc ggcttgagca tctgacttag actcaataac ttctgctctt agccgttcga ccatttcaaa tgcaggtcga tattgccctt ggcctttcat ccacgcagaa agtaactggc gctgccgcgc aaaagataac tcggataatt tcgttaaatc taaatagtca gctgaaccag aatattgcca atcatcttta agaacttctt ctaaaatttc agaagcatct tgcatcagat aactggttcg actcaaagct tgctgcattt ttggaaaacg gcttgttaaa aatggccaca gtgcttcacg acaccacacc ctatcgtaat ggttgtcata gttcattgga tcgtcaatat atttaacttc aagctgagca gtccataagg cgatttgctc acgtgttaaa tccaaaaagg ggcgccaaat tgtcatatct ttacggtaat caatagcctg catcgcagca agtccatcta ctcccgcacc tgacaataat cgcaaaataa ctgtttctgc ctgatcttgt tgatgatgag caagcaacag aatttcattt tcttgtaaat gttgctgata tgcttgataa cgcgcttgac gtgcttgtgc ttccagattg ccatttgcga tctgaacttt ttgaataata taaggaatat ttaatattgt tgcctgtgtg gcaaccactt ccgcccactc tgcactttga tcttgcagtt gatgatcaat atnnnnnata tggtggtggt ggtggtgagc catggtat
Expected Protein Sequence
MAHHHHHHIY IDHQLQDQSA EWAEVVATQA TILNIPYIIQ KVQIANGNLE AQARQARYQA YQQHLQENEI LLLAHHQQDQ AETVILRLLS GAGVDGLAAM QAIDYRKDMT IWRPFLDLTR EQIALWTAQL EVKYIDDPMN YDNHYDRVWC REALWPFLTS RFPKMQQALS RTSYLMQDAS EILEEVLKDD WQYSGSADYL DLTKLSELSF ARQRQLLSAW MKGQGQYRPA FEMVERLRAE VIESKSDAQA ALHWNQFYYV RYQNILYRLS KQIYFAETLN PVAAELEHSF KLEEKWQGAA GLFHVEYKKI GLSHSLLNKK LKIIRRQGGE KIHLYGRVGQ WPLKKAIQEA HILPWLRHTI QILVLDNVML GVFTPKGFWL AQSPYCEEGG WQPDLISHSC NLVNGEYSYG NSSEL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatttata ttgatcatca actgcaagat caaagtgcag agtgggcgga agtggttgcc acacaggcaa caatattaaa tattccttat attattcaaa aagttcagat cgcaaatggc aatctggaag cacaagcacg tcaagcgcgt tatcaagcat atcagcaaca tttacaagaa aatgaaattc tgttgcttgc tcatcatcaa caagatcagg cagaaacagt tattttgcga ttattgtcag gtgcgggagt agatggactt gctgcgatgc aggctattga ttaccgtaaa gatatgacaa tttggcgccc ctttttggat ttaacacgtg agcaaatcgc cttatggact gctcagcttg aagttaaata tattgacgat ccaatgaact atgacaacca ttacgatagg gtgtggtgtc gtgaagcact gtggccattt ttaacaagcc gttttccaaa aatgcagcaa gctttgagtc gaaccagtta tctgatgcaa gatgcttctg aaattttaga agaagttctt aaagatgatt ggcaatattc tggttcagct gactatttag atttaacgaa attatccgag ttatcttttg cgcggcagcg ccagttactt tctgcgtgga tgaaaggcca agggcaatat cgacctgcat ttgaaatggt cgaacggcta agagcagaag ttattgagtc taagtcagat gctcaagccg ctttacattg gaaccagttt tactatgtcc gttatcaaaa tatactctat aggttgagta aacagattta tttcgctgaa actttaaatc cggttgctgc tgaactagag cattcattta aattagaaga aaaatggcag ggcgcagctg gactgttcca tgttgagtat aaaaaaattg gtctaagtca ttctttattg aataaaaaat taaaaatcat tcgacgtcaa ggtggggaaa agattcacct atatggtcgt gttgggcaat ggcctttaaa aaaagcaatt caagaagcac atattttgcc ttggcttcgt catacaattc aaatattagt cttagataat gttatgcttg gggtttttac accgaaagga ttttggttgg ctcagtctcc atattgtgag gaaggagggt ggcaaccaga tttaatttct cattcttgta atctggtaaa tggcgaatat agttatggca acagcagtga attgtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat