AcbaC.17876.a

GMP synthase

CENTER ID: AcbaC.17876.a
ORGANISM: Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS: in PDB
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AcbaC.17876.a.B1.GE43940 full length 1 522
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
Structures
7SBC
DEPOSITED: 9/24/2021
DETERMINATION: XRay
CLONE: AcbaC.17876.a.B1.GE43940
PROTEIN: AcbaC.17876.a.B1.PW38920
External Resources
RESOURCE REFERENCE ID
GenBank: AKA33430.1
PATRIC ID: fig|470.1345.peg.3676
PGAT: acinetobacter:ABUW_3755
RefSeq: WP_000210750.1
UniProt: A0A0R1BBX7
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTTNTQITED RILILDFGSQ YSQLIARRVR EAGVYSEMYA FDMSEEDIRA FKPNGIILSG GPESVHEEGS PRAPQVVFEL GVPVLGICYG LQTMSEQLGG KVEPGTVHEF GYAEVDIVKR DQLIGNLQDR ENQLHVWMSH GDKVSQIPEG FTITASTPSC PVAAVSDETR RFYGVQFHPE VTHTAKGEEL LSNFVHKICG CGGLWTPEHI IDLRVEQLRE QIGNEKVLLG LSGGVDSSVV AALLHKAIGD QLTCVFVDNG LLRLNEGDQV MQMFAENMGI RVIRADAEAR FLNALAGVTD PEAKRKIIGR EFIEVFAEEA RKLDGVKFLA QGTIYPDVIE SAASKQGKAH VIKSHHNVGG LPDDLAFELV EPLRDLFKDE VRKLGTTLGL PHSMIYRHPF PGPGLGVRIL GEVKKEYADI LRLADDIFMQ ELRDSGWYDK TAQAFAVFQP VKSVGVVGDG RRYAWVIALR AVETVDFMTA RFAHLPYELV DKISTRIMNE IKDVSRVVYD VSSKPPATIE WE
NT Sequence
ATGACTACCA ATACTCAAAT TACTGAAGAT CGTATCCTGA TCCTGGATTT CGGCTCTCAA TATAGTCAGC TCATTGCACG TCGTGTACGT GAAGCTGGTG TTTACTCTGA AATGTATGCC TTTGATATGT CTGAAGAAGA CATTCGTGCT TTTAAGCCAA ACGGTATCAT TTTATCAGGT GGACCTGAGA GTGTGCATGA AGAGGGCAGC CCTCGCGCAC CGCAAGTGGT GTTTGAATTA GGCGTTCCAG TATTGGGTAT TTGCTATGGT TTACAAACCA TGTCTGAACA GTTAGGCGGT AAAGTTGAGC CGGGTACTGT ACATGAGTTT GGTTATGCCG AAGTTGATAT CGTTAAACGT GACCAACTTA TTGGCAACTT ACAAGACCGC GAAAACCAAC TACACGTTTG GATGAGCCAC GGCGACAAAG TGAGTCAGAT TCCAGAAGGT TTTACGATTA CTGCAAGCAC ACCAAGCTGT CCAGTTGCTG CTGTAAGTGA TGAAACACGC CGTTTTTACG GTGTTCAGTT CCACCCAGAA GTAACACACA CGGCAAAAGG TGAAGAGCTA CTTTCTAATT TCGTTCACAA AATTTGTGGA TGTGGTGGCC TTTGGACACC TGAACACATC ATCGATTTGC GTGTAGAGCA GTTACGTGAG CAAATTGGTA ACGAGAAAGT ACTTTTAGGC CTTTCTGGTG GTGTTGACTC ATCTGTTGTT GCTGCACTTT TACACAAAGC GATTGGTGAT CAATTAACAT GTGTATTTGT TGATAATGGT TTGCTTCGTT TAAACGAAGG TGACCAAGTG ATGCAAATGT TTGCTGAAAA CATGGGTATT CGTGTTATTC GTGCAGATGC AGAAGCGCGC TTCTTAAATG CCCTAGCTGG CGTTACCGAT CCTGAAGCGA AGCGTAAAAT TATTGGTCGT GAGTTTATTG AAGTATTTGC TGAAGAAGCA CGTAAACTTG ATGGCGTGAA ATTCTTGGCG CAAGGTACAA TTTACCCAGA CGTAATTGAA TCTGCTGCAA GCAAACAAGG TAAAGCACAC GTTATTAAAT CTCACCACAA TGTGGGTGGT TTACCAGACG ATTTAGCATT TGAATTGGTT GAGCCTTTAC GTGACTTGTT TAAAGATGAA GTACGTAAAT TAGGTACTAC ACTTGGCTTG CCACATAGCA TGATCTACCG TCATCCATTC CCGGGCCCAG GTTTAGGCGT TCGTATTTTG GGTGAAGTGA AAAAAGAATA TGCAGATATT CTTCGTCTTG CTGATGATAT CTTTATGCAA GAGCTTCGTG ACAGCGGTTG GTATGATAAA ACAGCTCAAG CATTTGCTGT CTTCCAACCT GTTAAATCAG TAGGTGTAGT CGGTGATGGC CGCCGTTATG CATGGGTGAT TGCGCTTCGT GCAGTTGAAA CAGTTGACTT TATGACAGCT CGTTTTGCAC ATTTACCATA CGAATTGGTT GATAAAATCT CAACACGCAT TATGAACGAA ATTAAAGATG TATCACGTGT GGTATACGAC GTGTCATCTA AACCACCAGC AACGATTGAG TGGGAATAA
Details for AcbaC.17876.a.B1.GE43940
HARVESTED ON: 12/3/2020
SEQUENCED ON: 12/16/2020
EXPECTED MW: 59kDa
OBSERVED MW: 59kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMT TNTQITEDRI LILDFGSQYS QLIARRVREA GVYSEMYAFD MSEEDIRAFK PNGIILSGGP ESVHEEGSPR APQVVFELGV PVLGICYGLQ TMSEQLGGKV EPGTVHEFGY AEVDIVKRDQ LIGNLQDREN QLHVWMSHGD KVSQIPEGFT ITASTPSCPV AAVSDETRRF YGVQFHPEVT HTAKGEELLS NFVHKICGCG GLWTPEHIID LRVEQLREQI GNEKVLLGLS GGVDSSVVAA LLHKAIGDQL TCVFVDNGLL RLNEGDQVMQ MFAENMGIRV IRADAEARFL NALAGVTDPE AKRKIIGREF IEVFAEEARK LDGVKFLAQG TIYPDVIESA ASKQGKAHVI KSHHNVGGLP DDLAFELVEP LRDLFKDEVR KLGTTLGLPH SMIYRHPFPG PGLGVRILGE VKKEYADILR LADDIFMQEL RDSGWYDKTA QAFAVFQPVK SVGVVGDGRR YAWVIALRAV ETVDFMTARF AHLPYELVDK ISTRIMNEIK DVSRVVYDVS SKP
Validated NT Sequence
atggctcacc accaccacca ccatatgact accaatactc aaattactga agatcgtatc ctgatcctgg atttcggctc tcaatatagt cagctcattg cacgtcgtgt acgtgaagct ggtgtttact ctgaaatgta tgcctttgat atgtctgaag aagacattcg tgcttttaag ccaaacggta tcattttatc aggtggacct gagagtgtgc atgaagaggg cagccctcgc gcaccgcaag tggtgtttga attaggcgtt ccagtattgg gtatttgcta tggtttacaa accatgtctg aacagttagg cggtaaagtt gagccgggta ctgtacatga gtttggttat gccgaagttg atatcgttaa acgtgaccaa cttattggca acttacaaga ccgcgaaaac caactacacg tttggatgag ccacggcgac aaagtgagtc agattccaga aggttttacg attactgcaa gcacaccaag ctgtccagtt gctgctgtaa gtgatgaaac acgccgtttt tacggtgttc agttccaccc agaagtaaca cacacggcaa aaggtgaaga gctactttct aatttcgttc acaaaatttg tggatgtggt ggcctttgga cacctgaaca catcatcgat ttgcgtgtag agcagttacg tgagcaaatt ggtaacgaga aagtactttt aggcctttct ggtggtgttg actcatctgt tgttgctgca cttttacaca aagcgattgg tgatcaatta acatgtgtat ttgttgataa tggtttgctt cgtttaaacg aaggtgacca agtgatgcaa atgtttgctg aaaacatggg tattcgtgtt attcgtgcag atgcagaagc gcgcttctta aatgccctag ctggcgttac cgatcctgaa gcgaagcgta aaattattgg tcgtgagttt attgaagtat ttgctgaaga agcacgtaaa cttgatggcg tgaaattctt ggcgcaaggt acaatttacc cagacgtaat tgaatctgct gcaagcaaac aaggtaaagc acacgttatt aaatctcacc acaatgtggg tggtttacca gacgatttag catttgaatt ggttgagcct ttacgtgact tgtttaaaga tgaagtacgt aaattaggta ctacacttgg cttgccacat agcatgatct accgtcatcc attcccgggc ccaggtttag gcgttcgtat tttgggtgaa gtgaaaaaag aatatgcaga tattcttcgt cttgctgatg atatctttat gcaagagctt cgtgacagcg gttggtatga taaaacagct caagcatttg ctgtcttcca acctgttaaa tcagtaggtg tagtcggtga tggccgccgt tatgcatggg tgattgcgct tcgtgcagtt gaaacagttg actttatgac agctcgtttt gcacatttac catacgaatt ggttgataaa atctcaacac gcattatgaa cgaaattaaa gatgtatcac gtgtggtata cgacgtgtca tctaaaccac c
Expected Protein Sequence
MAHHHHHHMT TNTQITEDRI LILDFGSQYS QLIARRVREA GVYSEMYAFD MSEEDIRAFK PNGIILSGGP ESVHEEGSPR APQVVFELGV PVLGICYGLQ TMSEQLGGKV EPGTVHEFGY AEVDIVKRDQ LIGNLQDREN QLHVWMSHGD KVSQIPEGFT ITASTPSCPV AAVSDETRRF YGVQFHPEVT HTAKGEELLS NFVHKICGCG GLWTPEHIID LRVEQLREQI GNEKVLLGLS GGVDSSVVAA LLHKAIGDQL TCVFVDNGLL RLNEGDQVMQ MFAENMGIRV IRADAEARFL NALAGVTDPE AKRKIIGREF IEVFAEEARK LDGVKFLAQG TIYPDVIESA ASKQGKAHVI KSHHNVGGLP DDLAFELVEP LRDLFKDEVR KLGTTLGLPH SMIYRHPFPG PGLGVRILGE VKKEYADILR LADDIFMQEL RDSGWYDKTA QAFAVFQPVK SVGVVGDGRR YAWVIALRAV ETVDFMTARF AHLPYELVDK ISTRIMNEIK DVSRVVYDVS SKPPATIEWE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacta ccaatactca aattactgaa gatcgtatcc tgatcctgga tttcggctct caatatagtc agctcattgc acgtcgtgta cgtgaagctg gtgtttactc tgaaatgtat gcctttgata tgtctgaaga agacattcgt gcttttaagc caaacggtat cattttatca ggtggacctg agagtgtgca tgaagagggc agccctcgcg caccgcaagt ggtgtttgaa ttaggcgttc cagtattggg tatttgctat ggtttacaaa ccatgtctga acagttaggc ggtaaagttg agccgggtac tgtacatgag tttggttatg ccgaagttga tatcgttaaa cgtgaccaac ttattggcaa cttacaagac cgcgaaaacc aactacacgt ttggatgagc cacggcgaca aagtgagtca gattccagaa ggttttacga ttactgcaag cacaccaagc tgtccagttg ctgctgtaag tgatgaaaca cgccgttttt acggtgttca gttccaccca gaagtaacac acacggcaaa aggtgaagag ctactttcta atttcgttca caaaatttgt ggatgtggtg gcctttggac acctgaacac atcatcgatt tgcgtgtaga gcagttacgt gagcaaattg gtaacgagaa agtactttta ggcctttctg gtggtgttga ctcatctgtt gttgctgcac ttttacacaa agcgattggt gatcaattaa catgtgtatt tgttgataat ggtttgcttc gtttaaacga aggtgaccaa gtgatgcaaa tgtttgctga aaacatgggt attcgtgtta ttcgtgcaga tgcagaagcg cgcttcttaa atgccctagc tggcgttacc gatcctgaag cgaagcgtaa aattattggt cgtgagttta ttgaagtatt tgctgaagaa gcacgtaaac ttgatggcgt gaaattcttg gcgcaaggta caatttaccc agacgtaatt gaatctgctg caagcaaaca aggtaaagca cacgttatta aatctcacca caatgtgggt ggtttaccag acgatttagc atttgaattg gttgagcctt tacgtgactt gtttaaagat gaagtacgta aattaggtac tacacttggc ttgccacata gcatgatcta ccgtcatcca ttcccgggcc caggtttagg cgttcgtatt ttgggtgaag tgaaaaaaga atatgcagat attcttcgtc ttgctgatga tatctttatg caagagcttc gtgacagcgg ttggtatgat aaaacagctc aagcatttgc tgtcttccaa cctgttaaat cagtaggtgt agtcggtgat ggccgccgtt atgcatgggt gattgcgctt cgtgcagttg aaacagttga ctttatgaca gctcgttttg cacatttacc atacgaattg gttgataaaa tctcaacacg cattatgaac gaaattaaag atgtatcacg tgtggtatac gacgtgtcat ctaaaccacc agcaacgatt gagtgggaat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat