AcbaC.17868.b
polyribonucleotide nucleotidyltransferase
| CENTER ID: | AcbaC.17868.b |
| ORGANISM: | Acinetobacter baumannii AB5075-UW |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| AcbaC.17868.b.B1.GE38764 | full length | 1 | 697 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| AcbaC.17868.b.B1.PS02399 | full length | 1 | 697 | ||
| AcbaC.17868.b.B1.PS38264 | full length | 1 | 697 |
Structures
6D6K
DEPOSITED: 4/21/2018
DETERMINATION: XRay
CLONE: AcbaC.17868.b.B1.GE38764
PROTEIN: AcbaC.17868.b.B1.PS38264
Publications by SSGCID
Towards a structome of Acinetobacter baumannii drug targets
Abendroth J, Barrett LK, Dranow DM, Edwards TE, Lorimer DD, Myler PJ, Phan I, Staker B, Subramanian S, Van Voorhis WC, Craig JC, Chun IKH, Barrett KF, Prentice RA, Tillery L, Shek P
Protein Sci. - 2020
volume 29, issue 3, pages 789-802
PMID: 31930600; PMCID: PMC7020997
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| GenBank: | AKA33212.1 |
| PATRIC ID: | fig|470.1345.peg.3453 |
| PGAT: | acinetobacter:ABUW_3518 |
| RefSeq: | WP_000061670.1 |
| UniProt: | V5VH92 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSMFNIVRKE FQFGQHQVVL ETGRVARQAN TVLITMGGVT VLVAVVAAPT AKAGQDFFPL TVNYQEKQYA AGRIPGGYGK REGRASEAET LISRLIDRPI RPLFPEGYYN EIQVTATVVS SDKTMEADIA AMLGTSAALA IAGTPFRGPI GAARVGLING EYVLNPNFEQ MAQSDLDLVV AGTESAVLMV ESEAKELSED QMLGAVLFGH DEMQIAIQAI NEFAAAAGAK PSDWVAPAHN EELRAKLKEA FEAKISEAYT IAVKQDRYAA LDALHAEAVA QFVPEEDVDG IADEVDYLFE DLKYRTVRDN ILSGKPRIDG RDTKTVRALD VQVGVLERAH GSALFTRGET QALVTTTLGN TRDALMVDTL AGTKTDNFML HYNFPAYSVG ETGRESGPKR REIGHGRLAR RGVQAVLPAA DRFPYVIRIV SDITESNGSS SMASVCGASL SLMDAGVPLK APVAGIAMGL VKEGERFAVL SDILGDEDHL GDMDFKVAGS ANGITALQMD IKIEGITEEI MEVALNQAFA GRMHILNEMN KVISRARPEI SMHAPTFEVI TINPDKIRDV IGKGGATIRQ ITEETKAAID IEDNGTVRVF GETKAAAKAA IAKIQAITAE VEPGKIYDGK VIRIVEFGAF VNIMPGTDGL LHISQISNER IANVTDVLKE GQEVKVQVQD VDNRGRIKLT MKDIEQA
NT Sequence
ATGTCAATGT TTAACATCGT TCGTAAAGAA TTCCAATTCG GTCAGCATCA AGTCGTTTTA GAAACGGGTC GCGTTGCACG TCAAGCAAAT ACAGTTTTAA TCACAATGGG TGGTGTAACT GTATTGGTAG CAGTAGTTGC TGCTCCAACA GCAAAAGCTG GTCAGGATTT TTTCCCTTTA ACAGTTAACT ATCAAGAAAA ACAATATGCA GCAGGTCGTA TCCCTGGTGG ATATGGTAAG CGTGAAGGTC GTGCTTCAGA AGCTGAAACT TTAATTTCAC GTCTTATTGA CCGTCCAATT CGTCCATTGT TCCCAGAAGG TTACTACAAC GAAATTCAGG TAACTGCAAC TGTGGTTTCT TCTGACAAAA CGATGGAAGC TGATATTGCA GCAATGTTAG GTACTTCTGC TGCGTTGGCA ATTGCAGGTA CTCCATTCCG TGGCCCAATC GGTGCAGCAC GTGTTGGTTT AATCAACGGC GAATATGTTT TAAACCCGAA TTTTGAGCAA ATGGCTCAGT CTGACCTTGA CCTTGTGGTT GCTGGTACAG AGTCTGCTGT ATTAATGGTT GAATCTGAAG CGAAAGAACT TTCAGAAGAC CAAATGTTAG GTGCGGTTCT TTTTGGTCAT GACGAAATGC AAATTGCAAT TCAAGCGATC AATGAATTTG CAGCTGCTGC TGGTGCGAAA CCATCTGACT GGGTTGCTCC TGCACACAAC GAAGAATTAC GTGCAAAATT AAAAGAAGCA TTTGAAGCAA AAATTTCAGA AGCTTATACC ATTGCAGTAA AACAAGATCG TTATGCAGCA TTAGATGCAC TTCATGCTGA AGCTGTTGCT CAATTCGTTC CTGAAGAAGA TGTAGACGGT ATTGCTGACG AAGTAGACTA CTTATTTGAA GATCTTAAAT ACCGTACTGT ACGTGACAAC ATTTTATCTG GTAAACCACG TATTGATGGC CGTGACACCA AAACTGTTCG TGCGTTAGAC GTACAAGTGG GCGTACTTGA GCGTGCACAT GGTTCTGCAT TATTTACACG TGGTGAAACT CAGGCGTTGG TAACAACGAC TTTGGGTAAT ACTCGTGATG CATTAATGGT TGATACTCTT GCTGGTACTA AAACTGACAA CTTCATGTTG CACTACAACT TCCCTGCTTA CTCAGTAGGT GAAACAGGCC GTGAATCTGG TCCTAAGCGT CGTGAAATTG GTCATGGTCG TTTAGCGCGT CGTGGTGTTC AAGCAGTTCT TCCTGCAGCT GACCGCTTCC CGTACGTTAT TCGTATTGTA TCTGACATTA CTGAATCTAA CGGTTCATCT TCAATGGCTT CTGTGTGTGG TGCGTCATTA TCACTTATGG ATGCAGGTGT TCCACTTAAA GCACCAGTTG CTGGTATCGC AATGGGCTTA GTGAAGGAAG GCGAGCGTTT CGCAGTACTT TCTGACATTT TAGGTGACGA AGACCACCTT GGTGACATGG ACTTTAAAGT AGCAGGTTCT GCAAACGGTA TTACTGCACT TCAAATGGAC ATCAAGATTG AAGGTATTAC TGAAGAAATT ATGGAAGTTG CATTAAACCA AGCATTTGCT GGCCGTATGC ATATCCTTAA TGAAATGAAC AAAGTAATTT CACGTGCTCG TCCTGAAATT TCAATGCACG CTCCAACATT CGAAGTGATT ACAATTAATC CGGATAAAAT CCGTGATGTT ATTGGTAAAG GTGGTGCAAC AATCCGTCAA ATCACTGAAG AAACTAAAGC TGCTATCGAT ATTGAAGATA ACGGTACAGT TCGCGTATTT GGTGAAACTA AAGCTGCTGC TAAAGCTGCA ATTGCGAAAA TTCAAGCGAT TACTGCTGAA GTTGAACCAG GTAAGATCTA CGACGGTAAA GTAATTCGTA TTGTAGAATT CGGTGCATTT GTAAACATCA TGCCGGGTAC TGATGGTCTT CTTCATATTT CGCAAATCTC TAATGAGCGT ATTGCGAACG TAACTGATGT TCTTAAAGAA GGTCAGGAAG TAAAAGTACA GGTTCAAGAC GTTGATAACC GTGGTCGTAT CAAATTGACT ATGAAAGATA TTGAGCAAGC ATAA
Details for AcbaC.17868.b.B1.GE38764
| HARVESTED ON: | 1/22/2015 |
| SEQUENCED ON: | 1/15/2015 |
| EXPECTED MW: | 76kDa |
| OBSERVED MW: | 76kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMS MFNIVRKEFQ FGQHQVVLET GRVARQANTV LITMGGVTVL VAVVAAPTAK AGQDFFPLTV NYQEKQYAAG RIPGGYGKRE GRASEAETLI SRLIDRPIRP LFPEGYYNEI QVTATVVSSD KTMEADIAAM LGTSAALAIA GTPFRGPIGA ARVGLINGEY VLNPNFEQMA QSDLDLVVAG TESAVLMVES EAKELSEDQM LGAVLFGHDE MQIAIQAINE FAAAAGAKPS DWVAPAHNEE LRAKLKEAFE AKISEAYTIA VKQDRYAALD ALHAEAVAQF VPEEDVDGIA DEVDYLFEDL KYRTVRDNIL SGKPRIDGRD TKTVRALDVQ VGVLERAHGS ALFTRGETQA LVTTTLGNTR DALMVDTLAG TKTDNFMLHY NFPAYSVGET GRESGPKRRE IGHGRLARRG VQAVLPAADR FPYVIRIVSD ITESNGSSSM ASVCGASLSL MDAGVPLKAP VAGIAMGLVK EGERFAVLSD ILGDEDHLGD MDFKVAGSAN GITALQMDIK IEGITEEIME VALNQAFAGR MHILNEMNKV ISRARPEISM HAPTFEVITI NPDKIRDVIG KGGATIRQIT EETKAAIDIE DNGTVRVFGE TKAAAKAAIA KIQAITAEVE PGKIYDGKVI RIVEFGAFVN IMPGTDGLLH ISQISNERIA NVTDVLKEGQ EVKVQVQDVD NRGRIKLTMK DIEQA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcaa tgtttaacat cgttcgtaaa gaattccaat tcggtcagca tcaagtcgtt ttagaaacgg gtcgcgttgc acgtcaagca aatacagttt taatcacaat gggtggtgta actgtattgg tagcagtagt tgctgctcca acagcaaaag ctggtcagga ttttttccct ttaacagtta actatcaaga aaaacaatat gcagcaggtc gtatccctgg tggatatggt aagcgtgaag gtcgtgcttc agaagctgaa actttaattt cacgtcttat tgaccgtcca attcgtccat tgttcccaga aggttactac aacgaaattc aggtaactgc aactgtggtt tcttctgaca aaacgatgga agctgatatt gcagcaatgt taggtacttc tgctgcgttg gcaattgcag gtactccatt ccgtggccca atcggtgcag cacgtgttgg tttaatcaac ggcgaatatg ttttaaaccc gaattttgag caaatggctc agtctgacct tgaccttgtg gttgctggta cagagtctgc tgtattaatg gttgaatctg aagcgaaaga actttcagaa gaccaaatgt taggtgcggt tctttttggt catgacgaaa tgcaaattgc aattcaagcg atcaatgaat ttgcagctgc tgctggtgcg aaaccatctg actgggttgc tcctgcacac aacgaagaat tacgtgcaaa attaaaagaa gcatttgaag caaaaatttc agaagcttat accattgcag taaaacaaga tcgttatgca gcattagatg cacttcatgc tgaagctgtt gctcaattcg ttcctgaaga agatgtagac ggtattgctg acgaagtaga ctacttattt gaagatctta aataccgtac tgtacgtgac aacattttat ctggtaaacc acgtattgat ggccgtgaca ccaaaactgt tcgtgcgtta gacgtacaag tgggcgtact tgagcgtgca catggttctg cattatttac acgtggtgaa actcaggcgt tggtaacaac gactttgggt aatactcgtg atgcattaat ggttgatact cttgctggta ctaaaactga caacttcatg ttgcactaca acttccctgc ttactcagta ggtgaaacag gccgtgaatc tggtcctaag cgtcgtgaaa ttggtcatgg tcgtttagcg cgtcgtggtg ttcaagcagt tcttcctgca gctgaccgct tcccgtacgt tattcgtatt gtatctgaca ttactgaatc taacggttca tcttcaatgg cttctgtgtg tggtgcgtca ttatcactta tggatgcagg tgttccactt aaagcaccag ttgctggtat cgcaatgggc ttagtgaagg aaggcgagcg tttcgcagta ctttctgaca ttttaggtga cgaagaccac cttggtgaca tggactttaa agtagcaggt tctgcaaacg gtattactgc acttcaaatg gacatcaaga ttgaaggtat tactgaagaa attatggaag ttgcattaaa ccaagcattt gctggccgta tgcatatcct taatgaaatg aacaaagtaa tttcacgtgc tcgtcctgaa atttcaatgc acgctccaac attcgaagtg attacaatta atccggataa aatccgtgat gttattggta aaggtggtgc aacaatccgt caaatcactg aagaaactaa agctgctatc gatattgaag ataacggtac agttcgcgta tttggtgaaa ctaaagctgc tgctaaagct gcaattgcga aaattcaagc gattactgct gaagttgaac caggtaagat ctacgacggt aaagtaattc gtattgtaga attcggtgca tttgtaaaca tcatgccggg tactgatggt cttcttcata tttcgcaaat ctctaatgag cgtattgcga acgtaactga tgttcttaaa gaaggtcagg aagtaaaagt acaggttcaa gacgttgata accgtggtcg tatcaaattg actatgaaag atattgagca agcatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for AcbaC.17868.b.B1.PS02399
| PURIFICATION DATe: | 5/13/2015 |
| CONCENTRATION: | 67.57mg/ml |
| OBSERVED MW: | 80kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 4 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMS MFNIVRKEFQ FGQHQVVLET GRVARQANTV LITMGGVTVL VAVVAAPTAK AGQDFFPLTV NYQEKQYAAG RIPGGYGKRE GRASEAETLI SRLIDRPIRP LFPEGYYNEI QVTATVVSSD KTMEADIAAM LGTSAALAIA GTPFRGPIGA ARVGLINGEY VLNPNFEQMA QSDLDLVVAG TESAVLMVES EAKELSEDQM LGAVLFGHDE MQIAIQAINE FAAAAGAKPS DWVAPAHNEE LRAKLKEAFE AKISEAYTIA VKQDRYAALD ALHAEAVAQF VPEEDVDGIA DEVDYLFEDL KYRTVRDNIL SGKPRIDGRD TKTVRALDVQ VGVLERAHGS ALFTRGETQA LVTTTLGNTR DALMVDTLAG TKTDNFMLHY NFPAYSVGET GRESGPKRRE IGHGRLARRG VQAVLPAADR FPYVIRIVSD ITESNGSSSM ASVCGASLSL MDAGVPLKAP VAGIAMGLVK EGERFAVLSD ILGDEDHLGD MDFKVAGSAN GITALQMDIK IEGITEEIME VALNQAFAGR MHILNEMNKV ISRARPEISM HAPTFEVITI NPDKIRDVIG KGGATIRQIT EETKAAIDIE DNGTVRVFGE TKAAAKAAIA KIQAITAEVE PGKIYDGKVI RIVEFGAFVN IMPGTDGLLH ISQISNERIA NVTDVLKEGQ EVKVQVQDVD NRGRIKLTMK DIEQA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCAA TGTTTAACAT CGTTCGTAAA GAATTCCAAT TCGGTCAGCA TCAAGTCGTT TTAGAAACGG GTCGCGTTGC ACGTCAAGCA AATACAGTTT TAATCACAAT GGGTGGTGTA ACTGTATTGG TAGCAGTAGT TGCTGCTCCA ACAGCAAAAG CTGGTCAGGA TTTTTTCCCT TTAACAGTTA ACTATCAAGA AAAACAATAT GCAGCAGGTC GTATCCCTGG TGGATATGGT AAGCGTGAAG GTCGTGCTTC AGAAGCTGAA ACTTTAATTT CACGTCTTAT TGACCGTCCA ATTCGTCCAT TGTTCCCAGA AGGTTACTAC AACGAAATTC AGGTAACTGC AACTGTGGTT TCTTCTGACA AAACGATGGA AGCTGATATT GCAGCAATGT TAGGTACTTC TGCTGCGTTG GCAATTGCAG GTACTCCATT CCGTGGCCCA ATCGGTGCAG CACGTGTTGG TTTAATCAAC GGCGAATATG TTTTAAACCC GAATTTTGAG CAAATGGCTC AGTCTGACCT TGACCTTGTG GTTGCTGGTA CAGAGTCTGC TGTATTAATG GTTGAATCTG AAGCGAAAGA ACTTTCAGAA GACCAAATGT TAGGTGCGGT TCTTTTTGGT CATGACGAAA TGCAAATTGC AATTCAAGCG ATCAATGAAT TTGCAGCTGC TGCTGGTGCG AAACCATCTG ACTGGGTTGC TCCTGCACAC AACGAAGAAT TACGTGCAAA ATTAAAAGAA GCATTTGAAG CAAAAATTTC AGAAGCTTAT ACCATTGCAG TAAAACAAGA TCGTTATGCA GCATTAGATG CACTTCATGC TGAAGCTGTT GCTCAATTCG TTCCTGAAGA AGATGTAGAC GGTATTGCTG ACGAAGTAGA CTACTTATTT GAAGATCTTA AATACCGTAC TGTACGTGAC AACATTTTAT CTGGTAAACC ACGTATTGAT GGCCGTGACA CCAAAACTGT TCGTGCGTTA GACGTACAAG TGGGCGTACT TGAGCGTGCA CATGGTTCTG CATTATTTAC ACGTGGTGAA ACTCAGGCGT TGGTAACAAC GACTTTGGGT AATACTCGTG ATGCATTAAT GGTTGATACT CTTGCTGGTA CTAAAACTGA CAACTTCATG TTGCACTACA ACTTCCCTGC TTACTCAGTA GGTGAAACAG GCCGTGAATC TGGTCCTAAG CGTCGTGAAA TTGGTCATGG TCGTTTAGCG CGTCGTGGTG TTCAAGCAGT TCTTCCTGCA GCTGACCGCT TCCCGTACGT TATTCGTATT GTATCTGACA TTACTGAATC TAACGGTTCA TCTTCAATGG CTTCTGTGTG TGGTGCGTCA TTATCACTTA TGGATGCAGG TGTTCCACTT AAAGCACCAG TTGCTGGTAT CGCAATGGGC TTAGTGAAGG AAGGCGAGCG TTTCGCAGTA CTTTCTGACA TTTTAGGTGA CGAAGACCAC CTTGGTGACA TGGACTTTAA AGTAGCAGGT TCTGCAAACG GTATTACTGC ACTTCAAATG GACATCAAGA TTGAAGGTAT TACTGAAGAA ATTATGGAAG TTGCATTAAA CCAAGCATTT GCTGGCCGTA TGCATATCCT TAATGAAATG AACAAAGTAA TTTCACGTGC TCGTCCTGAA ATTTCAATGC ACGCTCCAAC ATTCGAAGTG ATTACAATTA ATCCGGATAA AATCCGTGAT GTTATTGGTA AAGGTGGTGC AACAATCCGT CAAATCACTG AAGAAACTAA AGCTGCTATC GATATTGAAG ATAACGGTAC AGTTCGCGTA TTTGGTGAAA CTAAAGCTGC TGCTAAAGCT GCAATTGCGA AAATTCAAGC GATTACTGCT GAAGTTGAAC CAGGTAAGAT CTACGACGGT AAAGTAATTC GTATTGTAGA ATTCGGTGCA TTTGTAAACA TCATGCCGGG TACTGATGGT CTTCTTCATA TTTCGCAAAT CTCTAATGAG CGTATTGCGA ACGTAACTGA TGTTCTTAAA GAAGGTCAGG AAGTAAAAGT ACAGGTTCAA GACGTTGATA ACCGTGGTCG TATCAAATTG ACTATGAAAG ATATTGAGCA AGCATGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT
Details for AcbaC.17868.b.B1.PS38264
| PURIFICATION DATe: | 4/26/2017 |
| CONCENTRATION: | 31mg/ml |
| OBSERVED MW: | 80kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | SEC Buffer: 20mM HEPES pH 7.0, 300mM NaCl, 5% glycerol, 1mM TCEP |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 3 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMS MFNIVRKEFQ FGQHQVVLET GRVARQANTV LITMGGVTVL VAVVAAPTAK AGQDFFPLTV NYQEKQYAAG RIPGGYGKRE GRASEAETLI SRLIDRPIRP LFPEGYYNEI QVTATVVSSD KTMEADIAAM LGTSAALAIA GTPFRGPIGA ARVGLINGEY VLNPNFEQMA QSDLDLVVAG TESAVLMVES EAKELSEDQM LGAVLFGHDE MQIAIQAINE FAAAAGAKPS DWVAPAHNEE LRAKLKEAFE AKISEAYTIA VKQDRYAALD ALHAEAVAQF VPEEDVDGIA DEVDYLFEDL KYRTVRDNIL SGKPRIDGRD TKTVRALDVQ VGVLERAHGS ALFTRGETQA LVTTTLGNTR DALMVDTLAG TKTDNFMLHY NFPAYSVGET GRESGPKRRE IGHGRLARRG VQAVLPAADR FPYVIRIVSD ITESNGSSSM ASVCGASLSL MDAGVPLKAP VAGIAMGLVK EGERFAVLSD ILGDEDHLGD MDFKVAGSAN GITALQMDIK IEGITEEIME VALNQAFAGR MHILNEMNKV ISRARPEISM HAPTFEVITI NPDKIRDVIG KGGATIRQIT EETKAAIDIE DNGTVRVFGE TKAAAKAAIA KIQAITAEVE PGKIYDGKVI RIVEFGAFVN IMPGTDGLLH ISQISNERIA NVTDVLKEGQ EVKVQVQDVD NRGRIKLTMK DIEQA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCAA TGTTTAACAT CGTTCGTAAA GAATTCCAAT TCGGTCAGCA TCAAGTCGTT TTAGAAACGG GTCGCGTTGC ACGTCAAGCA AATACAGTTT TAATCACAAT GGGTGGTGTA ACTGTATTGG TAGCAGTAGT TGCTGCTCCA ACAGCAAAAG CTGGTCAGGA TTTTTTCCCT TTAACAGTTA ACTATCAAGA AAAACAATAT GCAGCAGGTC GTATCCCTGG TGGATATGGT AAGCGTGAAG GTCGTGCTTC AGAAGCTGAA ACTTTAATTT CACGTCTTAT TGACCGTCCA ATTCGTCCAT TGTTCCCAGA AGGTTACTAC AACGAAATTC AGGTAACTGC AACTGTGGTT TCTTCTGACA AAACGATGGA AGCTGATATT GCAGCAATGT TAGGTACTTC TGCTGCGTTG GCAATTGCAG GTACTCCATT CCGTGGCCCA ATCGGTGCAG CACGTGTTGG TTTAATCAAC GGCGAATATG TTTTAAACCC GAATTTTGAG CAAATGGCTC AGTCTGACCT TGACCTTGTG GTTGCTGGTA CAGAGTCTGC TGTATTAATG GTTGAATCTG AAGCGAAAGA ACTTTCAGAA GACCAAATGT TAGGTGCGGT TCTTTTTGGT CATGACGAAA TGCAAATTGC AATTCAAGCG ATCAATGAAT TTGCAGCTGC TGCTGGTGCG AAACCATCTG ACTGGGTTGC TCCTGCACAC AACGAAGAAT TACGTGCAAA ATTAAAAGAA GCATTTGAAG CAAAAATTTC AGAAGCTTAT ACCATTGCAG TAAAACAAGA TCGTTATGCA GCATTAGATG CACTTCATGC TGAAGCTGTT GCTCAATTCG TTCCTGAAGA AGATGTAGAC GGTATTGCTG ACGAAGTAGA CTACTTATTT GAAGATCTTA AATACCGTAC TGTACGTGAC AACATTTTAT CTGGTAAACC ACGTATTGAT GGCCGTGACA CCAAAACTGT TCGTGCGTTA GACGTACAAG TGGGCGTACT TGAGCGTGCA CATGGTTCTG CATTATTTAC ACGTGGTGAA ACTCAGGCGT TGGTAACAAC GACTTTGGGT AATACTCGTG ATGCATTAAT GGTTGATACT CTTGCTGGTA CTAAAACTGA CAACTTCATG TTGCACTACA ACTTCCCTGC TTACTCAGTA GGTGAAACAG GCCGTGAATC TGGTCCTAAG CGTCGTGAAA TTGGTCATGG TCGTTTAGCG CGTCGTGGTG TTCAAGCAGT TCTTCCTGCA GCTGACCGCT TCCCGTACGT TATTCGTATT GTATCTGACA TTACTGAATC TAACGGTTCA TCTTCAATGG CTTCTGTGTG TGGTGCGTCA TTATCACTTA TGGATGCAGG TGTTCCACTT AAAGCACCAG TTGCTGGTAT CGCAATGGGC TTAGTGAAGG AAGGCGAGCG TTTCGCAGTA CTTTCTGACA TTTTAGGTGA CGAAGACCAC CTTGGTGACA TGGACTTTAA AGTAGCAGGT TCTGCAAACG GTATTACTGC ACTTCAAATG GACATCAAGA TTGAAGGTAT TACTGAAGAA ATTATGGAAG TTGCATTAAA CCAAGCATTT GCTGGCCGTA TGCATATCCT TAATGAAATG AACAAAGTAA TTTCACGTGC TCGTCCTGAA ATTTCAATGC ACGCTCCAAC ATTCGAAGTG ATTACAATTA ATCCGGATAA AATCCGTGAT GTTATTGGTA AAGGTGGTGC AACAATCCGT CAAATCACTG AAGAAACTAA AGCTGCTATC GATATTGAAG ATAACGGTAC AGTTCGCGTA TTTGGTGAAA CTAAAGCTGC TGCTAAAGCT GCAATTGCGA AAATTCAAGC GATTACTGCT GAAGTTGAAC CAGGTAAGAT CTACGACGGT AAAGTAATTC GTATTGTAGA ATTCGGTGCA TTTGTAAACA TCATGCCGGG TACTGATGGT CTTCTTCATA TTTCGCAAAT CTCTAATGAG CGTATTGCGA ACGTAACTGA TGTTCTTAAA GAAGGTCAGG AAGTAAAAGT ACAGGTTCAA GACGTTGATA ACCGTGGTCG TATCAAATTG ACTATGAAAG ATATTGAGCA AGCATGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT