AcbaC.17867.a

electron transfer flavoprotein subunit alpha

CENTER ID: AcbaC.17867.a
ORGANISM: Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AcbaC.17867.a.B1.GE38762 full length 1 310
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AcbaC.17867.a.B1.PS02439 full length 1 310
AcbaC.17867.a.B1.PS38105 full length 1 310

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|470.1345.peg.923
GenBank: AKA30713.1
PGAT: acinetobacter:ABUW_0959
RefSeq: WP_000025988.1
UniProt: X2KZW3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSILVIADHN NQVLNGATLN VVAAAQKIGG DITVLVAGSG AQAVADAAAK VAGVSKVLLA DNAAYANQLA ENVAGLVADL AKGYKYVLAA STTTGKNILP RVAALLDVSM ITDIISVESA NTFKRPIYAG NAIATVQSDE AIIVGTVRGT AFDPVAAEGG SAAVETVGEV KDAGVSKFVS EEIVKLDRPE LTAARIVVSG GRGVGSGENY HKVLDPLADK LGAAQGASRA AVDAGFVPND FQVGQTGKIV APELYIAVGI SGAIQHLAGM KDSKVIVAIN KDEEAPINSV ADYWLVGDLN AVVPELVSKL
NT Sequence
ATGAGTATTT TAGTTATCGC TGACCACAAC AACCAAGTAC TTAACGGTGC TACTTTAAAC GTTGTTGCTG CAGCTCAAAA AATCGGTGGT GATATTACTG TATTAGTTGC TGGTTCAGGC GCTCAAGCAG TTGCTGACGC TGCTGCTAAA GTTGCTGGTG TGAGCAAAGT ATTACTTGCT GACAACGCTG CTTATGCAAA CCAATTAGCT GAAAACGTAG CTGGCTTAGT TGCTGACTTG GCTAAAGGTT ACAAATACGT TTTAGCTGCT TCTACAACTA CTGGTAAGAA CATTCTTCCA CGTGTTGCTG CGCTTCTTGA TGTAAGCATG ATCACAGACA TTATTTCTGT TGAATCGGCT AACACATTCA AGCGTCCAAT CTACGCTGGT AACGCAATTG CAACTGTTCA ATCTGACGAA GCAATCATTG TTGGTACTGT TCGTGGTACA GCATTTGACC CAGTTGCTGC GGAAGGTGGT TCAGCTGCTG TTGAAACAGT TGGCGAAGTA AAAGATGCTG GTGTTTCTAA GTTTGTATCT GAAGAAATCG TTAAACTTGA TCGTCCAGAA TTAACAGCTG CTCGTATTGT AGTTTCTGGT GGTCGTGGTG TAGGTTCTGG TGAGAACTAC CACAAAGTAC TTGACCCATT AGCTGACAAG CTTGGTGCAG CACAAGGTGC TTCACGTGCA GCAGTTGATG CAGGCTTCGT ACCTAACGAC TTCCAAGTTG GTCAAACTGG TAAAATCGTT GCACCTGAGC TTTACATTGC TGTAGGTATT TCTGGTGCGA TCCAGCACTT GGCTGGTATG AAAGATTCTA AAGTTATTGT TGCTATCAAC AAAGACGAAG AAGCGCCAAT CAACAGCGTT GCTGACTACT GGTTAGTTGG TGATTTGAAC GCTGTAGTGC CAGAGTTGGT ATCTAAGCTT TAA
Details for AcbaC.17867.a.B1.GE38762
HARVESTED ON: 1/22/2015
SEQUENCED ON: 1/15/2015
EXPECTED MW: 33kDa
OBSERVED MW: 33kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMS ILVIADHNNQ VLNGATLNVV AAAQKIGGDI TVLVAGSGAQ AVADAAAKVA GVSKVLLADN AAYANQLAEN VAGLVADLAK GYKYVLAAST TTGKNILPRV AALLDVSMIT DIISVESANT FKRPIYAGNA IATVQSDEAI IVGTVRGTAF DPVAAEGGSA AVETVGEVKD AGVSKFVSEE IVKLDRPELT AARIVVSGGR GVGSGENYHK VLDPLADKLG AAQGASRAAV DAGFVPNDFQ VGQTGKIVAP ELYIAVGISG AIQHLAGMKD SKVIVAINKD EEAPINSVAD YWLVGDLNAV VPELVSKL
Validated NT Sequence
aaagcttaga taccaactct ggcactacag cgttcaaatc accaactaac cagtagtcag caacgctgtt gattggcgct tcttcgtctt tgttgatagc aacaataact ttagaatctt tcataccagc caagtgctgg atcgcaccag aaatacctac agcaatgtaa agctcaggtg caacgatttt accagtttga ccaacttgga agtcgttagg tacgaagcct gcatcaactg ctgcacgtga agcaccttgt gctgcaccaa gcttgtcagc taatgggtca agtactttgt ggtagttctc accagaacct acaccacgac caccagaaac tacaatacga gcagctgtta attctggacg atcaagttta acgatttctt cagatacaaa cttagaaaca ccagcatctt ttacttcgcc aactgtttca acagcagctg aaccaccttc cgcagcaact gggtcaaatg ctgtaccacg aacagtacca acaatgattg cttcgtcaga ttgaacagtt gcaattgcgt taccagcgta gattggacgc ttgaatgtgt tagccgattc aacagaaata atgtctgtga tcatgcttac atcaagaagc gcagcaacac gtggaagaat gttcttacca gtagttgtag aagcagctaa aacgtatttg taacctttag ccaagtcagc aactaagcca gctacgtttt cagctaattg gtttgcataa gcagcgttgt cagcaagtaa tactttgctc acaccagcaa ctttagcagc agcgtcagca actgcttgag cgcctgaacc agcaactaat acagtaatat caccaccgat tttttgagct gcagcaacaa cgtttaaagt agcaccgtta agtacttggt tgttgtggtc agcgataact aaaatactca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHMS ILVIADHNNQ VLNGATLNVV AAAQKIGGDI TVLVAGSGAQ AVADAAAKVA GVSKVLLADN AAYANQLAEN VAGLVADLAK GYKYVLAAST TTGKNILPRV AALLDVSMIT DIISVESANT FKRPIYAGNA IATVQSDEAI IVGTVRGTAF DPVAAEGGSA AVETVGEVKD AGVSKFVSEE IVKLDRPELT AARIVVSGGR GVGSGENYHK VLDPLADKLG AAQGASRAAV DAGFVPNDFQ VGQTGKIVAP ELYIAVGISG AIQHLAGMKD SKVIVAINKD EEAPINSVAD YWLVGDLNAV VPELVSKL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagta ttttagttat cgctgaccac aacaaccaag tacttaacgg tgctacttta aacgttgttg ctgcagctca aaaaatcggt ggtgatatta ctgtattagt tgctggttca ggcgctcaag cagttgctga cgctgctgct aaagttgctg gtgtgagcaa agtattactt gctgacaacg ctgcttatgc aaaccaatta gctgaaaacg tagctggctt agttgctgac ttggctaaag gttacaaata cgttttagct gcttctacaa ctactggtaa gaacattctt ccacgtgttg ctgcgcttct tgatgtaagc atgatcacag acattatttc tgttgaatcg gctaacacat tcaagcgtcc aatctacgct ggtaacgcaa ttgcaactgt tcaatctgac gaagcaatca ttgttggtac tgttcgtggt acagcatttg acccagttgc tgcggaaggt ggttcagctg ctgttgaaac agttggcgaa gtaaaagatg ctggtgtttc taagtttgta tctgaagaaa tcgttaaact tgatcgtcca gaattaacag ctgctcgtat tgtagtttct ggtggtcgtg gtgtaggttc tggtgagaac taccacaaag tacttgaccc attagctgac aagcttggtg cagcacaagg tgcttcacgt gcagcagttg atgcaggctt cgtacctaac gacttccaag ttggtcaaac tggtaaaatc gttgcacctg agctttacat tgctgtaggt atttctggtg cgatccagca cttggctggt atgaaagatt ctaaagttat tgttgctatc aacaaagacg aagaagcgcc aatcaacagc gttgctgact actggttagt tggtgatttg aacgctgtag tgccagagtt ggtatctaag ctttgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for AcbaC.17867.a.B1.PS02439
PURIFICATION DATe: 6/2/2015
CONCENTRATION: 46.4mg/ml
OBSERVED MW: 34kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 3
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS ILVIADHNNQ VLNGATLNVV AAAQKIGGDI TVLVAGSGAQ AVADAAAKVA GVSKVLLADN AAYANQLAEN VAGLVADLAK GYKYVLAAST TTGKNILPRV AALLDVSMIT DIISVESANT FKRPIYAGNA IATVQSDEAI IVGTVRGTAF DPVAAEGGSA AVETVGEVKD AGVSKFVSEE IVKLDRPELT AARIVVSGGR GVGSGENYHK VLDPLADKLG AAQGASRAAV DAGFVPNDFQ VGQTGKIVAP ELYIAVGISG AIQHLAGMKD SKVIVAINKD EEAPINSVAD YWLVGDLNAV VPELVSKL
Validated NT Sequence
aaagcttaga taccaactct ggcactacag cgttcaaatc accaactaac cagtagtcag caacgctgtt gattggcgct tcttcgtctt tgttgatagc aacaataact ttagaatctt tcataccagc caagtgctgg atcgcaccag aaatacctac agcaatgtaa agctcaggtg caacgatttt accagtttga ccaacttgga agtcgttagg tacgaagcct gcatcaactg ctgcacgtga agcaccttgt gctgcaccaa gcttgtcagc taatgggtca agtactttgt ggtagttctc accagaacct acaccacgac caccagaaac tacaatacga gcagctgtta attctggacg atcaagttta acgatttctt cagatacaaa cttagaaaca ccagcatctt ttacttcgcc aactgtttca acagcagctg aaccaccttc cgcagcaact gggtcaaatg ctgtaccacg aacagtacca acaatgattg cttcgtcaga ttgaacagtt gcaattgcgt taccagcgta gattggacgc ttgaatgtgt tagccgattc aacagaaata atgtctgtga tcatgcttac atcaagaagc gcagcaacac gtggaagaat gttcttacca gtagttgtag aagcagctaa aacgtatttg taacctttag ccaagtcagc aactaagcca gctacgtttt cagctaattg gtttgcataa gcagcgttgt cagcaagtaa tactttgctc acaccagcaa ctttagcagc agcgtcagca actgcttgag cgcctgaacc agcaactaat acagtaatat caccaccgat tttttgagct gcagcaacaa cgtttaaagt agcaccgtta agtacttggt tgttgtggtc agcgataact aaaatactca tatggtggtg gtggtggtga gccat
Expressed Protein Sequence
MAHHHHHHMS ILVIADHNNQ VLNGATLNVV AAAQKIGGDI TVLVAGSGAQ AVADAAAKVA GVSKVLLADN AAYANQLAEN VAGLVADLAK GYKYVLAAST TTGKNILPRV AALLDVSMIT DIISVESANT FKRPIYAGNA IATVQSDEAI IVGTVRGTAF DPVAAEGGSA AVETVGEVKD AGVSKFVSEE IVKLDRPELT AARIVVSGGR GVGSGENYHK VLDPLADKLG AAQGASRAAV DAGFVPNDFQ VGQTGKIVAP ELYIAVGISG AIQHLAGMKD SKVIVAINKD EEAPINSVAD YWLVGDLNAV VPELVSKL
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGTA TTTTAGTTAT CGCTGACCAC AACAACCAAG TACTTAACGG TGCTACTTTA AACGTTGTTG CTGCAGCTCA AAAAATCGGT GGTGATATTA CTGTATTAGT TGCTGGTTCA GGCGCTCAAG CAGTTGCTGA CGCTGCTGCT AAAGTTGCTG GTGTGAGCAA AGTATTACTT GCTGACAACG CTGCTTATGC AAACCAATTA GCTGAAAACG TAGCTGGCTT AGTTGCTGAC TTGGCTAAAG GTTACAAATA CGTTTTAGCT GCTTCTACAA CTACTGGTAA GAACATTCTT CCACGTGTTG CTGCGCTTCT TGATGTAAGC ATGATCACAG ACATTATTTC TGTTGAATCG GCTAACACAT TCAAGCGTCC AATCTACGCT GGTAACGCAA TTGCAACTGT TCAATCTGAC GAAGCAATCA TTGTTGGTAC TGTTCGTGGT ACAGCATTTG ACCCAGTTGC TGCGGAAGGT GGTTCAGCTG CTGTTGAAAC AGTTGGCGAA GTAAAAGATG CTGGTGTTTC TAAGTTTGTA TCTGAAGAAA TCGTTAAACT TGATCGTCCA GAATTAACAG CTGCTCGTAT TGTAGTTTCT GGTGGTCGTG GTGTAGGTTC TGGTGAGAAC TACCACAAAG TACTTGACCC ATTAGCTGAC AAGCTTGGTG CAGCACAAGG TGCTTCACGT GCAGCAGTTG ATGCAGGCTT CGTACCTAAC GACTTCCAAG TTGGTCAAAC TGGTAAAATC GTTGCACCTG AGCTTTACAT TGCTGTAGGT ATTTCTGGTG CGATCCAGCA CTTGGCTGGT ATGAAAGATT CTAAAGTTAT TGTTGCTATC AACAAAGACG AAGAAGCGCC AATCAACAGC GTTGCTGACT ACTGGTTAGT TGGTGATTTG AACGCTGTAG TGCCAGAGTT GGTATCTAAG CTTTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT
Details for AcbaC.17867.a.B1.PS38105
PURIFICATION DATe: 11/3/2016
CONCENTRATION: 13.51mg/ml
OBSERVED MW: 34kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: SEC
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 6
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS ILVIADHNNQ VLNGATLNVV AAAQKIGGDI TVLVAGSGAQ AVADAAAKVA GVSKVLLADN AAYANQLAEN VAGLVADLAK GYKYVLAAST TTGKNILPRV AALLDVSMIT DIISVESANT FKRPIYAGNA IATVQSDEAI IVGTVRGTAF DPVAAEGGSA AVETVGEVKD AGVSKFVSEE IVKLDRPELT AARIVVSGGR GVGSGENYHK VLDPLADKLG AAQGASRAAV DAGFVPNDFQ VGQTGKIVAP ELYIAVGISG AIQHLAGMKD SKVIVAINKD EEAPINSVAD YWLVGDLNAV VPELVSKL
Validated NT Sequence
aaagcttaga taccaactct ggcactacag cgttcaaatc accaactaac cagtagtcag caacgctgtt gattggcgct tcttcgtctt tgttgatagc aacaataact ttagaatctt tcataccagc caagtgctgg atcgcaccag aaatacctac agcaatgtaa agctcaggtg caacgatttt accagtttga ccaacttgga agtcgttagg tacgaagcct gcatcaactg ctgcacgtga agcaccttgt gctgcaccaa gcttgtcagc taatgggtca agtactttgt ggtagttctc accagaacct acaccacgac caccagaaac tacaatacga gcagctgtta attctggacg atcaagttta acgatttctt cagatacaaa cttagaaaca ccagcatctt ttacttcgcc aactgtttca acagcagctg aaccaccttc cgcagcaact gggtcaaatg ctgtaccacg aacagtacca acaatgattg cttcgtcaga ttgaacagtt gcaattgcgt taccagcgta gattggacgc ttgaatgtgt tagccgattc aacagaaata atgtctgtga tcatgcttac atcaagaagc gcagcaacac gtggaagaat gttcttacca gtagttgtag aagcagctaa aacgtatttg taacctttag ccaagtcagc aactaagcca gctacgtttt cagctaattg gtttgcataa gcagcgttgt cagcaagtaa tactttgctc acaccagcaa ctttagcagc agcgtcagca actgcttgag cgcctgaacc agcaactaat acagtaatat caccaccgat tttttgagct gcagcaacaa cgtttaaagt agcaccgtta agtacttggt tgttgtggtc agcgataact aaaatactca tatggtggtg gtggtggtga gccat
Expressed Protein Sequence
MAHHHHHHMS ILVIADHNNQ VLNGATLNVV AAAQKIGGDI TVLVAGSGAQ AVADAAAKVA GVSKVLLADN AAYANQLAEN VAGLVADLAK GYKYVLAAST TTGKNILPRV AALLDVSMIT DIISVESANT FKRPIYAGNA IATVQSDEAI IVGTVRGTAF DPVAAEGGSA AVETVGEVKD AGVSKFVSEE IVKLDRPELT AARIVVSGGR GVGSGENYHK VLDPLADKLG AAQGASRAAV DAGFVPNDFQ VGQTGKIVAP ELYIAVGISG AIQHLAGMKD SKVIVAINKD EEAPINSVAD YWLVGDLNAV VPELVSKL
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGTA TTTTAGTTAT CGCTGACCAC AACAACCAAG TACTTAACGG TGCTACTTTA AACGTTGTTG CTGCAGCTCA AAAAATCGGT GGTGATATTA CTGTATTAGT TGCTGGTTCA GGCGCTCAAG CAGTTGCTGA CGCTGCTGCT AAAGTTGCTG GTGTGAGCAA AGTATTACTT GCTGACAACG CTGCTTATGC AAACCAATTA GCTGAAAACG TAGCTGGCTT AGTTGCTGAC TTGGCTAAAG GTTACAAATA CGTTTTAGCT GCTTCTACAA CTACTGGTAA GAACATTCTT CCACGTGTTG CTGCGCTTCT TGATGTAAGC ATGATCACAG ACATTATTTC TGTTGAATCG GCTAACACAT TCAAGCGTCC AATCTACGCT GGTAACGCAA TTGCAACTGT TCAATCTGAC GAAGCAATCA TTGTTGGTAC TGTTCGTGGT ACAGCATTTG ACCCAGTTGC TGCGGAAGGT GGTTCAGCTG CTGTTGAAAC AGTTGGCGAA GTAAAAGATG CTGGTGTTTC TAAGTTTGTA TCTGAAGAAA TCGTTAAACT TGATCGTCCA GAATTAACAG CTGCTCGTAT TGTAGTTTCT GGTGGTCGTG GTGTAGGTTC TGGTGAGAAC TACCACAAAG TACTTGACCC ATTAGCTGAC AAGCTTGGTG CAGCACAAGG TGCTTCACGT GCAGCAGTTG ATGCAGGCTT CGTACCTAAC GACTTCCAAG TTGGTCAAAC TGGTAAAATC GTTGCACCTG AGCTTTACAT TGCTGTAGGT ATTTCTGGTG CGATCCAGCA CTTGGCTGGT ATGAAAGATT CTAAAGTTAT TGTTGCTATC AACAAAGACG AAGAAGCGCC AATCAACAGC GTTGCTGACT ACTGGTTAGT TGGTGATTTG AACGCTGTAG TGCCAGAGTT GGTATCTAAG CTTTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT